نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش آموخته دکتری اصلاح نباتات، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل

2 استاد تمام دانشگاه محقق اردبیلی، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل

3 دانشیار دانشگاه محقق اردبیلی، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل

4 استادیار پژوهشکده کشاورزی گچساران مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی کهگیلویه و بویراحمد

چکیده

با توجه به وجود تنش سرما در مناطق سردسیر کشور لزوم گزینش ژنوتیپ‌هایی با پتانسیل عملکرد بالا و سایر ویژگی‌های مناسب زراعی بیش از پیش آشکار می‌باشد. هدف این مطالعه بررسی تنوع لاین‌های گندم دوروم در شرایط تنش یخ‌زدگی و شناسایی لاین‌های مقاوم به سرما در گندم دوروم بر اساس برخی صفات مورفوفیزیولوژیکی و تعیین رابطه بین صفات گندم دوروم و تحمل به سرما بود. آزمایشات یخ‌زدگی و آزمایشگاهی در گلخانه و آزمایشگاه اصلاح نباتات دانشگاه محقق اردبیلی در سال‌ 98 بر روی 45 لاین امیدبخش گندم دوروم به‌صورت آزمایش فاکتوریل در قالب طرح بلوک کامل تصادفی با 3 تکرار به اجرا درآمد. تمامی لاین‌های مورد بررسی قادر به تحمل تنش بودند. در مجموع لاین‌های 34، 36 و 37 بیشترین درصد زنده‌مانی و لاین‌های 1، 23 و 25 کمترین میزان زنده‌مانی را نشان دادند. صفات LT50، درصد زنده‌مانی، ارتفاع، وزن تر اندام هوایی، وزن خشک اندام هوایی، وزن اشباع اندام هوایی، تعداد برگ، محتوای آب نسبی و نشت الکترولیت مورد بررسی قرار گرفتند. لاین‌هایی که در گروه لاین‌های پابلند خوشه‌بندی شدند از نظر صفت LT50 نیز در گروه لاین‌های مقاوم قرار گرفتند. در تجزیه خوشه‌ای لاین‌ها در شرایط شاهد، تنش 8- و 12- درجه سانتی‌گراد لاین‌ها در چهار گروه و در شرایط تنش 10- درجه سانتی‌گراد لاین‌ها در 5 گروه قرار گرفتند. صفات وزن تر اندام هوایی، تعداد برگ، محتوای نسبی آب برگ و هدایت الکتریکی نقش مهمی در میانگین گروه‌ها داشتند.در تجزیه به عامل‌ها در سطوح شاهد و تنش 8- درجه سانتی‌گراد چهار عامل و برای سطوح تنش 10- و 12- درجه سانتی‌گراد سه عامل برای تفسیر داده‌ها انتخاب شد. با توجه به نتایج نهایی مطالعه، لاین‌های 17، 24، 27، 35، 38 و 40 لاین‌های مقاوم شناخته شدند. لاین‌های 3، 12، 18، 20 و 44 حساس شناخته شدند.

کلیدواژه‌ها

موضوعات

 
 Abbaszadeh, F., 2012. Investigating osmotic stress tolerance in lentils and its relationship with molecular markers, master's thesis in plant breeding, Faculty of Agriculture, Mohaghegh Ardabili University. [In Persian].
Ahmed, H.G.M.D., Zeng, Y., Yang, X., Anwaar, H. A., Mansha, M. Z., Hanif, C.M.S., Ikram, K., Ullah, A., Alghanem, S.M.S., 2020. Conferring drought-tolerant wheat genotypes through morpho physiological and chlorophyll indices at seedling stage. Saudi Journal of Biological Sciences. 27,  116–2123. https://doi.org/10.1016/j.sjbs.2020.06.019
Ali, M., Jensen, C., Mogensen, V., Andersen, M., Henson, I., 1999. Root signalling and osmotic adjustment during intermittent soil drying sustain grain yield of field grown wheat. Field Crops Research. 62, 35–52. https://doi.org/10.1023/A:1004535819197
Aroca, R., Porcel, R., Ruiz-Lozano, J.M., 2012. Regulation of root water uptake under abiotic stress conditions. Journal of Experimental Botany. 63, 43–57. https://doi.org/10.1093/jxb/err266
Augspurger, C.K., 2013. Reconstructing patterns of temperature, phenology, and frost damage over 124 years: Spring damage risk is increasing. Ecology. 94, 41–50. https://doi.org/10.1890/12-0200.1
Auld, D.L., Ditterline, R.L., Murrayand, G.A., Swensen, J.B., 1983. Screening peas for winter hardiness under field and laboratory conditions. Crop Science. 23, 85-88.  https://doi.org/10.2135/cropsci1983.0011183X002300010024x
Azizi, H., Nezami, A., Nassiri, M., Khazaee, H.R., 2007. Evaluation of cold tolerance in wheat (Triticum aestivum L.) cultivars under controlled conditions. Iranian Journal of Field Crops Research, 6, 109-120. [In Persian]. https://doi.org/10.22067/GSC.V6I2.2441
Bagheri, A., Nezami, A., Soltani, M., 2000. Improvement of cold-loving legumes to tolerate droughts. Translation of the organization of research, Education and Promotion of Agriculture. 445p. [In Persian].
Bohn, M., Lüthje, S., Sperling, P., Heinz, E., Dörffling, K., 2007. Plasma membrane lipid alterations induced by cold acclimation and abscisic acid treatment of winter wheat seedlings differing in frost resistance. Journal of Plant Physiology. 164, 146–156. https://doi.org/10.1016/j.jplph.2005.12.008
Bonjean, A.P., Angus, W.J., van Ginkel, M., 2016. The world wheat book: A history of wheat breeding Vol. 3. Lavoisier, Paris.
Bridger, G.M., Falk, D.E., Mckersie, B.D., Smith, D.L., 1996. Crown freezing tolerance and field winter survival of winter cereals in eastern Canada. Crop Science. 36, 150-157.  https://doi.org/10.2135/cropsci1996.0011183X003600010027x
Campos, P.S., Quartin, V., Ramalho, J.C., Nunes, M.A., 2003. Electrolyte leakage and lipid degradation account for cold sensitivity in leaves of Coffea sp. Plants. Journal of Plant Physiology, 160, 283–292. https://doi.org/10.1078/0176-1617-00833
Crimp, S.J., Zheng, B., Khimashia, N., Gobbett, D.L. Chapman, S., Howden, M., 2016. Recent changes in southern Australian frost occurrence: implications for wheat production risk. Crop Pasture Science. 67, 801–811. https://doi.org/10.1071/CP16056
Dolferus, R., Ji, X., Richards, R.A., 2011. Abiotic stress and control of grain number in cereals. Plant Science. 181, 331–341.  https://doi.org/10.1016/j.plantsci.2011.05.015
Dubcovsky, J., Dvorak, J., 2007. Genome plasticity a key factor in the success of polyploid wheat under domestication. Science. 316, 1862–1866. https://doi.org/10.1126/science.1143986
Duvnjak J., Lonˇcari´c, A., Brkljaˇci´c, L., Šamec, D., Šarˇcevi´c, H., Salopek-Sondi B., Špani´c, V., 2023. Morpho-Physiological and Hormonal Response of Winter Wheat Varieties to Drought Stress at Stem Elongation and Anthesis Stages. Plants. 12, 418. https://doi.org/10.3390/plants12030418
Eugenia, M., Nunes, S., Ray Smith, G., 2003. Electrolyte leakage assay capable of quantifying freezing resistance in rose clover. Crop Science. 43, 1349-1357. https://doi.org/10.2135/cropsci2003.1349
Farahani, H.A., Moaveni, P., Maroufi, K., 2011. Effect of seed size on seedling production in wheat (Triticum aestivum L.). Advances in Environmental Biology. 5, 1711–1716. Corpus ID: 33567938
Food and Agriculture Organization of the United Nations (FAOSTAT). 2023. Data of Crop Production. Available online: https://www.statista.com/statistics
Fuller, M.P., Fuller, A., Kaniouras, M., Christophers, S.J., Fredericks, T., 2007. The freezing characteristics of wheat at ear emergence. Europen Journal of Agronomy. 26, 435–441. https://doi.org/10.1016/j.eja.2007.01.001
Hassan, M.A., Xiang, C., Farooq, M., Muhammad, N., Yan, Z., Hui, X., Jincai, L., 2021. Cold stress in wheat: Plant acclimation responses and management strategies. Frontiers in Plant Science. 12, 676884. https://doi.org/10.3389/fpls.2021.676884
Hanna, B., Bischofa, J.C., 2004. Direct cell injury associated with eutectic crystallization during freezing. Cryobio. 48: 8-21. https://doi.org/10.1016/j.cryobiol.2003.11.002
Henry, R.J., Rangan, P., Furtado, A., 2016. Functional cereals for production in new and variable climates. Current Opinion in Plant Biology. 30, 11–18. https://doi.org/10.1016/j.pbi.2015.12.008
Izadi Darbandi, E., Nezami, A., Hasan Beigi R., Janalizadeh Qazvini, M., 2016. The effect of freezing stress on the percentage of electrolyte leakage and the survival of Descurainia sophia L. seedlings. Iranian Agricultural Research Journal. 14, 369-379. [In persian].
Janowiak, F., Maas, B., Dörffling K., 2002. Importance of abscisic acid for chilling tolerance of maize seedlings. Journal of Plant Physiology. 159, 635–643. https://doi.org/10.1078/0176-1617-0638
Karabina, K., Leonardi, E., 2016. Turkey grain and feed annual report. TR6015. USDA Foreign Agric. Serv., Washington, DC.
Kodra, E., Steinhaeuser, K., Ganguly, A.R., 2011. Persisting cold extremes under 21st-century warming scenarios. Geophysical Research Letters. 38, 47–103. https://doi.org/10.1029/2011GL047103
Kratsch, H.A., Wise, R.R., 2000. The ultrastructure of chilling stress. Plant, Cell Environ. 23, 337–350. https://doi.org/10.1046/j.1365-3040.2000.00560.x
Lin, M., Huybers, P., 2012. Reckoning wheat yield trends. Environtal Research Letters. 7, 024016. https://doi.org/10.1088/1748-9326/7/2/024016
Liu, B., Wang, X.Y., Cao, Y., Arora, R., Zhou, H., Xia, Y.P., 2020. Factors affecting freezing tolerance: A comparative transcriptomics study between field and artificial cold acclimations in overwintering evergreens. Plant Journal. 103, 2279–2300. https://doi.org/10.1111/tpj.14899
Los, D.A., Mironov, K.S., Allakhverdiev, S.I., 2013. Regulatory role of membrane fluidity in gene expression and physiological functions. Photosynth. Resarch. 116, 489–509. https://doi.org/10.1007/s11120-013-9823-4
Lugojan, C., Ciulca, S., 2011. Evaluation of relative water content in winter wheat. Journal of Horticulture and Postharvest Research. 15, 173–177. Corpus ID: 83378835
Mahfoozi, S., Limin, A.E., Fowler, D.B., 2001. Influence of vernalization and photoperiod responses on cold hardiness in winter cereals. Crop Science. 41, 1006-1011. https://doi.org/10.2135/cropsci2001.4141006x
Martínez-Moreno, F., Solís, I., Noguero, D., Blanco, A., Ozberk, I., Nsarellah, N., Elias, E., Mylonas, I., Soriano, J.M., 2020. Durum wheat in the Mediterranean Rim: historical evolution and genetic resources. Genetic Resources and Crop Evolution, 67, 1415–1436. https://doi.org/10.1007/s10722-020-00913-8
McKersie, B.D., Leshem, Y.Y., 1994. Stress and stress cropping in cultivated plants. Kluwer Academic Publishers. The Netherlands.
Mireshghi, A., Khalilzadeh, G.R., 2002. Evaluation of some physiological traits related to cold resistance in 22 bread wheat genotypes. Summary of the articles of the third conference on reducing losses caused by cold and freezing of agricultural and garden plants in the country. P. 61. Vice President of Agriculture, Plant Protection Organization [In persian].
Mirmohammadi Meibodi, A.M., 2000. Physiological and genetic aspects of cold stress and frost of agricultural plants. Golban Publications, Isfahan. [In persian].
Mohammadi, R., Haghparast, R., 2022. Durum wheat: production, nutritional value and economic importance. Cereal Biotechnology and Biochemistry. 1, 414-45. [In persian]. https://doi.org/10.22126/cbb.2022.8385.1021
Muhammad, A.H., Chen, X., Muhammad, F., Noor, M., Zhang, Y., Xu, H., Ke, Y., Attiogbe, K. B., Zhang, L., Li, J., 2021. Cold stress in wheat: Plant acclimation responses and management strategies. Frontiers in Plant Science. https://doi.org/10.3389/fpls.2021.676884
Murray, L.E., Rowley, N.,  Dawes, I.W.,  Johnston, G.C., Singer, R.A., 1998. A yeast glutamine tRNA signals nitrogen status for regulation of dimorphic growth and sporulation. Proceedings of the National Academy of Sciences of the United States of America. 95, 8619-24. https://doi.org/10.1073/pnas.95.15.8619
Nemati, M., 2011. Investigation of drought resistance in rapeseed and its relationship with molecular markers, master's thesis in plant breeding, Faculty of Agriculture, Mohaghegh Ardabili University [In persian].
Nezami, A., Borzoi, A., Jahani kondari, M., Azizi, M., Sharif, A., 2016. Electrolyte leakage as an indicator  of freezing injury in colza      (Brassica napus L.). Iranian Journal of Agricultural Research. 5(1), 175-167 [In persian]. https://doi.org/10.22067/gsc.v5i1.907
Nezami, A., Nabati, J., Borzooei, A., Kamandi, A., Masomi, A., Salehi, M., 2010. Evaluation of freezing tolerance in barley (Hordeum vulgar L.) cultivars at seedling stage under controlled conditions. Environmental Stresses in Crop Sciences. 3, 9-22. [In Persian]. https://doi.org/10.22077/escs.2010.79
Prasil, I.T., Prasilov, P., Marik, P., 2007. Comparative study of direct and indirect evaluations of frost tolerance in barley. Field Crops Research, 102, 1-8. https://doi.org/10.1016/j.fcr.2006.12.012 
Rashed Mohassel, M.H., Nezami, A., Bagheri, A., Hajmohammadnia Ghalibaf, K., Bannayan Aval, M., 2009. Evaluation of freezing tolerance of two fennel (Foeniculum vulgar L.) ecotypes under controlled conditions. Journal of Herbs, Spices and Medicinal Plants. 15, 131–140. https://doi.org/10.1080/10496470903139249
Ritchie, S.W.,  Nguyen, H.T., 1990. Leaf water content and gas exchange parameters of two wheat genotypes differing in drought resistance. Crop Science, 30, 105-111. https://doi.org/10.2135/cropsci1990.0011183X003000010025x
Ruelland, E., Zachowski, A., 2010. How plants sense temperature. Environtal Experience of Botany. 69, 225–232. https://doi.org/10.1016/j.envexpbot.2010.05.011
Saeedi, M., Abdoli, M., Eliyasi, P., 2019. Investigation of cold tolerance in some bread wheat genotypes at the seedling stage under laboratory conditions. Second Term Wheat Research Journal. 1, 36-52. [In persian].
Seiler., G.J., Stafford, R.E., 1985. Factor analysis of component of yield in guar. Crop Science. 25, 905-908.  https://doi.org/10.2135/cropsci1985.0011183X002500060003x
Seo, P.J., Kim, M.J., Park, J.Y., Kim, S.Y., Jeon, J., Lee, Y.H., 2010. Cold activation of a plasma membrane-tethered NAC transcription factor induces a pathogen resistance response in Arabidopsis. Plant Journal. 61, 661–671. https://doi.org/10.1111/j.1365-313X.2009.04091.x
Sio-Se Mardeh, A., Mohammadi, K., Rouhi, E., Agha Alikhani, M., Mokhtasi Bidgoli, A., 2010. Physiological response of different wheat genotypes to cold stress. Electronic Journal of Crop Production. 2, 93-112. [In persian]. https://dorl.net/dor/20.1001.1.2008739.1388.2.4.6.0
Skendži´c, S., Zovko, M., Leši´c, V., Pajaˇc Živkovi´c, I., Lemi´c, D., 2023. Detection and Evaluation of Environmental Stress in Winter Wheat Using Remote and Proximal Sensing Methods and Vegetation Indices—A Review. Diversity. 15, 481. https://doi.org/10.3390/d15040481
Tahmasab-Ali, M., 2013. The study of osmotic stress tolerance in wheat cultivars and its relationship with protein markers, master's thesis in plant breeding, Faculty of Agriculture, Mohaghegh Ardabili University. [In persian].
Teutonica, R.A., Palta, J.P., Osborn, T.C., 1993. In vitro freezing tolerance in relation to winter survival of rapeseed cultivars. Crop Sci. 33, 103-107. https://doi.org/10.2135/cropsci1993.0011183X003300010019x
Thakur, P., Kumar, S., Malik, J.A., Berger, J.D., Nayyar, H., 2010. Cold stress effects on reproductive development in grain crops: An overview. Environmental Experience Botany. 67, 429–443. https://doi.org/10.1016/j.envexpbot.2009.09.004
Theocharis, A., Clément, C., Barka, E.A., 2012. Physiological and molecular changes in plants grown at low temperatures. Planta. 235, 1091–1105. https://doi.org/10.1007/s00425-012-1641-y
Uemura, M., Tominaga, Y., Nakagawara, C., Shigematsu, S., Minami, A., Kawamura, Y., 2006. Responses of plasma membrane to low temperature. Physiologia Planta. 126, 81-89. https://doi.org/10.1111/j.1399-3054.2005.00594.x
Whaley, J.M., Kirby, E.J.M. Spink, J.H., Foulkes, M.J., Sparkes, D.L., 2004. Frost damage to winter wheat in the UK: the effect of plant population density. European Journal of Agronomy. 21, 105–115. https://doi.org/10.1016/S1161-0301(03)00090-X
Wheeler, T., 2013. Von Braun, J. Climate change impacts on global food security. Science. 341, 508–513. https://doi.org/10.1126/science.1239402
Zheng, B., Chapman, S.C., Christopher, J.T., Frederiks, T.M., Chenu, K., 2015. Frost trends and their estimated impact on yield in the Australian wheatbelt. Journal of Expperimental Botany. https://doi.org/10.1093/jxb/erv163