نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی دکتری رشته فیزیولوژی گیاهان زراعی، گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل
2 استاد گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل
10.22077/escs.2024.6592.2231
چکیده
به منظور بررسی تاثیر شوری، ورمی کمپوست و متانول بر فعالیت آنزیم های آنتی اکسیدان، اسمولیت های سازگار و برخی صفات تریتیکاله، آزمایشی به صورت فاکتوریل در قالب طرح پایه بلوک های کامل تصادفی در سه تکرار در گلخانه تحقیقاتی دانشگاه محقق اردبیلی در سال 1401 اجرا شد. فاکتورهای مورد بررسی شامل سه سطح شوری (عدم اعمال شوری به عنوان شاهد، شوری 50 و 100 میلی مولار از نمک سدیم کلرید)، دو سطح ورمی کمپوست (بدون کاربرد به عنوان شاهد و کاربرد ورمی کمپوست) و سه سطح متانول (محلول‌پاشی با آب به‌عنوان شاهد، محلول پاشی 15 و 30 درصد حجمی متانول) بود. نتایج نشان داد کاربرد همزمان ورمی کمپوست و متانول 30 درصد حجمی در سطح شوری 100 میلی مولار فعالیت آنزیم پراکسیداز (73.4 درصد)، پلی فنل اکسیداز (89.6 درصد)، قند محلول (20.3 درصد)، پروتئین (38 درصد)، پرولین (101.9 درصد) و محتوای نسبی آب (20.6 درصد) را نسبت به تیمار عدم کاربرد ورمی کمپوست و متانول درهمین سطح شوری افزایش داد. کاربرد ورمی کمپوست محتوای مالون دی آلدهید و پراکسید هیدروژن را (به ترتیب 9 و 20 درصد) در مقایسه با عدم کاربرد ورمی کمپوست کاهش داد. کاربرد متانول 30 درصد حجمی محتوای مالون دی آلدهید و پراکسیدهیدروژن را (به ترتیب 40 و 55 درصد) در مقایسه با عدم کاربرد متانول کاهش داد. بیش ترین عملکرد دانه از کاربرد همزمان ورمی کمپوست و متانول 30 درصد حجمی در شرایط عدم شوری به دست آمد. براساس نتایج این تحقیق، به نظر می رسد کاربرد ورمی کمپوست و متانول به دلیل بهبود فعالیت آنزیم های آنتی اکسیدانی و اسمولیت های سازگار می تواند موجب افزایش عملکرد دانه تریتیکاله تحت تنش شوری شود.
کلیدواژه‌ها
موضوعات
Abbasian, A., Mirshekari, B., Safarzade Vishekaei, M.N., Rashidi, V., Aminpanah, H., 2016. Effects of the foliar application of methanol on the yield and growth of rice (Oryza sativa cv. Shiroudi). Ciencia e investigación agrarian. 43, 17-24. https://doi.org/10.4067/S0718-16202016000100002
Ahmad, A., Aslam, Z., Hussain, S., Bibi, A., Khaliq, A., Javed, T., Hussain, S., Alotaibi, S.S., Kalaji, H.M., Telesinski, A., Boonthai Iwai, C., Kumar, U., 2022. Rice straw vermicompost enriched with cellulolytic microbes ameliorate the negative effect of drought in wheat through modulating the morpho-physiological attributes. Frontiers in Environmental Science. 10, 902999. https://doi.org/10.3389/fenvs.2022.902999
Alamer, K.H., Perveen, S., Khaliq, A., Zia Ul Haq, M., Ibrahim, M.U., Ijaz, B., 2022. Mitigation of salinity stress in maize seedlings by the application of vermicompost and sorghum water extracts. Plants. 11, 2548. https://doi.org/10.3390/plants11192548
Alexieva, V., Sergiev, I., Mapelli, S., Karanov, E., 2001. The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. Plant, Cell and Environment. 24, 1337-1344. https://doi.org/10.1046/j.1365-3040.2001.00778.x
Alharbi, K., Hafez, E.M., Omara, A.E. D., Osman, H.S., 2023. Mitigating osmotic stress and enhancing developmental productivity processes in cotton through integrative use of vermicompost and cyanobacteria. Plants. 12, 1872. https://doi.org/10.3390/plants12091872
Aliyar, S., Aliasgharzad, N., Dabbagh Mohammadi Nasab, A., Ostan, S., 2021. The effect of vermicompost application on growth and water relationships of Quinoa plant under salinity stress conditions. Journal of Agricultural Science and Sustainable Production. 31, 131-147. [In Persian with English summary].
Alshiekheid, M.A., Dwiningsih, Y., Alkahtani, J., 2023. Analysis of morphological, physiological, and biochemical traits of salt stress tolerance in Asian rice cultivars at different stages. Preprints.org, 1-19. https://doi.org/10.20944/preprints202303.0251.v1
Ashour, H.A., Heider, S.M., Soliman, M.M., 2023. Morphological and physiological responses of Calliandra haematocephala to water salinity stress and vermicompost. Ornamental Horticulture. 29, 150-162. https://doi.org/10.1590/2447-536X.v29i2.2555
Atarodi Asl, E., Khalilvand Behrouzyar, E., 2020. Effect of methanol and salicylic acid foliar application on some of physiological traits of winter wheat under limited irrigation. Environmental Stresses in Crop Sciences. 13, 815-827. [In Persian with English summary]. https://doi.org/10.22077/escs.2020.2199.1549
Bates, L.S., Waldren, R.A., Teare, I.D., 1973. Rapid determination of free proline for water-stress studies. Plant and Soil. 39, 205-207. https://doi.org/10.1007/BF00018060
Behrouzyar, E.K., Yarnia, M., 2016. Effects of methanol foliar application on nutrient content and RWC of sugar beet under water deficit stress. Bangladesh Journal of Botany. 45, 1069-1074.
Beyk-Khormizi, A., Hosseini Sarghein, S., Sarafraz-Ardakani, M.R., Moshtaghioun, S.M., Mousavi-Kouhi, S.M., Ganjeali, A., 2022. Ameliorating effect of vermicompost on Foeniculum vulgare under saline condition. Journal of Plant Nutrition.46, 1601-1615. https://doi.org/10.1080/01904167.2022.2092513
Bradford, M.M., 1976. A rapid and sensitive for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry. 72, 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
Bziouech, S.A., Dhen, N., Helaoui, S., Ammar, I.B., Dridi, B.A. M., 2022. Effect of vermicompost soil additive on growth performance, physiological and biochemical responses of tomato plants (Solanum lycopersicum L. var. Firenze) to salt stress. Emirates Journal of Food and Agriculture. 34, 316-328. https://doi.org/10.9755/ejfa.2022.v34.i4.2844
Darakeh, S.A.S.S., Weisany, W., Tahir, N.A.R., Schenk, P.M., 2022. Physiological and biochemical responses of black cumin to vermicompost and plant biostimulants: Arbuscular mycorrhizal and plant growth-promoting rhizobacteria. Industrial Crops and Products. 188, 115557. https://doi.org/10.1016/j.indcrop.2022.115557
Dorokhov, Y.L., Sheshukova, E.V., Komarova, T.V., 2018. Methanol in plant life. Frontiers in Plant Science. 9, 1-16. https://doi.org/10.15835/nsb13210949
Dubios, M., Gilles, K.A., Hamilton, J.K., Roberts, P.A., Smith, F., 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry. 28, 350-356. https://doi.org/10.1021/ac60111a017
Ezzat, A.S., Badway, A.S., Abdelkader, A.E., 2019. Sequenced vermicompost, glycine betaine, proline treatments elevate salinity tolerance in potatoes. Middle East Journal of Agriculture Research. 8, 126-138.
Ghassemi-Golezani, K., Abdoli, S., 2022. Physiological and biochemical responses of medicinal plants to salt stress. In Environmental Challenges and Medicinal Plants: Sustainable Production Solutions under Adverse Conditions. Springer International Publishing. 153-181p. https://doi.org/10.1007/978-3-030-92050-0_6
Golebiowska-Paluch, G., Dyda, M., 2023. The genome regions associated with abiotic and biotic stress tolerance, as well as other important breeding traits in triticale. Plants. 12, 619. https://doi.org/10.3390/plants12030619
Hassanpouraghdam, M.B., Mehrabani, L.V., Rahvar, M.R., Khoshmaram, L., Soltanbeigi, A., 2022. Mollifying salt depression on Anethum graveolens L. by the foliar prescription of Nano-Zn, KNO3, Methanol, and Graphene Oxide. Journal of Soil Science and Plant Nutrition. 22, 2000-2012. [In Persian with English summary].
Hossinzadeh, S.R., Salimi, A., Ganjeali, A., Ahmadpour, R., 2015. Effects of foliar application of methanol on biochemical characteristics and antioxidant enzyme activity of chickpea (Cicer arietinum L.) under drought stress. Iranian Journal of Plant Physiology and Biochemistry. 1, 17-30. [In Persian with English summary].
Ivanova, E.G., Dornina, N.V and Trotsenko, Y.A., 2001. Aerobic methylobacteria are capable of synthesizing auxins. Journal of Microbiology. 70, 392-397. https://doi.org/10.1023/A:1010469708107
Joshi, R., Singh, J., Vig, A.P., 2015. Vermicompost as an effective organic fertilizer and biocontrol agent: effect on growth, yield and quality of plants. Reviews in Environmental Science and Bio/Technology. 14, 137-159. https://doi.org/10.1007/s11157-014-9347-1
Keles, Y., Öncel, I., 2004. Growth and solute composition in two wheat species experiencing combined influence of stress conditions. Russian Journal of Plant Physiology. 51, 203-209. https://doi.org/10.1023/B:RUPP.0000019215.20500.6e
Khalilzadeh, R., Seyd Sharifi, R., Pirzad, A., 2020. Mitigation of drought stress in pot marigold (Calendula officinalis) plant by foliar application of methanol. Journal of Plant Physiology and Breeding. 10, 71-84. [In Persian with English summary].
Kiani, R., Arzani, A., Mirmohammady Maibody, S.A.M., 2021. Polyphenols, flavonoids, and antioxidant activity involved in salt tolerance in wheat, Aegilops cylindrica and their amphidiploids. Frontiers in plant science. 12, 646221. https://doi.org/10.3389/fpls.2021.646221
Kostopoulou, P., Barbayiannis, N., Noitsakis, B., 2010. Water relations of yellow sweetclover under the synergy of drought and selenium addition. Plant and Soil. 330, 65-71. https://doi.org/10.1007/s11104-009-0176-x
Lahbouki, S., Anli, M., El Gabardi, S., Ait-El-Mokhtar, M., Ben-Laouane, R., Boutasknit, A., Ati-Rahou, Y., Outzaourhit, A., Wahbi, S., Douira, A., Meddich, A., 2022. Evaluation of arbuscular mycorrhizal fungi and vermicompost supplementation on growth, phenolic content and antioxidant activity of prickly pear cactus (Opuntia ficus-indica). Plant Biosystems-An International Journal Dealing with All Aspects of Plant Biology. 156, 882-892. https://doi.org/10.1080/11263504.2021.1947408
Liu, Y., Cao, X., Yue, L., Wang, C., Tao, M., Wang, Z., Xing, B., 2022. Foliar-applied cerium oxide nanomaterials improve maize yield under salinity stress: Reactive oxygen species homeostasis and rhizobacteria regulation. Environmental Pollution. 299P, 118900. https://doi.org/10.1016/j.envpol.2022.118900
Makkar, C., Singh, J., Parkash, C., Singh, S., Vig, A. P., Dhaliwal, S.S., 2023. Vermicompost acts as bio-modulator for plants under stress and non-stress conditions. Environment, Development and Sustainability. 25, 2006-2057. https://doi.org/10.1007/s10668-022-02132-w
Mirakhori, M., Paknejad, F., Moradi, M., Ardakani, M.R., Zahedi, H., Nazeri, P., 2009. Effect of drought stress and methanol on yield and yield components of Soybean Max L. American Journal of Biochemistry and Biotechnology. 5, 162-169. [In Persian with English summary].
Mohammadi Kalesar Lou, S., Seyed Sharifi, R., Narimani, H., 2022. Effects of flavobacterim, vermicompost and humic acid on antioxidant enzymes activity and some biochemical traits of triticale under soil salinity conditions. Journal of Crop Production. 15, 183-202. [In Persian with English summary].
Mushtaq, Z., Faizan, S., Gulzar, B., 2020. Salt stress, its impacts on plants and the strategies plants are employing against it: A review. Journal of Applied Biology and Biotechnology. 8, 81-91. https://doi.org/10.7324/JABB.2020.80315
Naboulsi, I., Ben Mrid, R., Ennoury, A., Zouaoui, Z., Nhiri, M., Ben Bakrim, W., Yasri, A., Aboulmouhajir, A., 2022. Crataegus oxyacantha extract as a biostimulant to enhance tolerance to salinity in tomato plants. Plants. 11, 1283. https://doi.org/10.3390/plants11101283
Naghdi Badi, H.A., Tolyat Abulhassani, S.M., Nazari, M., Mehrafarin, A., 2017. Phytochemical Response of Sweet Basil (Ocimum basilicum) to Application of Methanol Biostimulant and Iron Nano-chelate. Journal of Medicinal Plants. 16, 91-106. [In Persian with English summary].
Narimani, H., Seyed Sharifi, R., 2023. Effect of mycorrhiza and methanol on grain filling components, dry matter remobilization and yield of barley under soil salinity conditions. Environmental Stresses in Crop Sciences. 16, 383-401. [In Persian with English summary].
Nonomura, A.M., Benson, A.A., 1992. The path of carbon in photosynthesis: improved crop yields with methanol. Proceedings of the National Academy of Sciences. 89, 9794-9798. https://doi.org/10.1073/pnas.89.20.9794
Phooi, C.L., Azman, E.A., Ismail, R., 2022. Do it yourself: Humic acid. Pertanika Journal of Tropicle Agricultural Science. 45, 547-564. https://doi.org/10.47836/pjtas.45.3.01
Ramirez, I.F., Dorta, V., Espinoza, E., Jimenez, A., Mercado, H., 2006. Effects of foliar and root applications of methanol on the growth of Arabidopsis, tobacco and tomato plants. Journal of Plant Growth Regulation. 25, 30- 44. https://doi.org/10.1007/s00344-005-0027-9
Redondo-Gómez, S., Romano-Rodríguez, E., Mesa-Marín, J., Sola-Elías, C., Mateos-Naranjo, E., 2022. Consortia of plant-growth-promoting rhizobacteria isolated from halophytes improve the response of Swiss chard to soil salinization. Agronomy. 12, 468. https://doi.org/10.3390/agronomy11081609
Rehman, S.U., De Castro, F., Aprile, A., Benedetti, M., Fanizzi, F.P., 2023. Vermicompost: Enhancing plant growth and combating abiotic and biotic stress. Agronomy. 13, 1134.
Sahab, S., Suhani, I., Srivastava, V., Chauhan, P.S., Singh, R.P., Prasad, V., 2021. Potential risk assessment of soil salinity to agroecosystem sustainability: Current status and management strategies. Science of the Total Environment. 764, 144164. https://doi.org/10.1016/j.scitotenv.2020.144164
Saneinejad, A.A., Tohidi, M., Habibi Khaniani, B., Sadeghi, M., Khoramian, M., 2019. The effect of methanol foliar application on some physiological traits of cowpea bean (Vigna unguiculata L.) under drought stress conditions. Journal of Agronomy and Plant Breeding. 15, 45-61 [In Persian with English summary].
Singh, P., Kumar, V., Sharma, J., Saini, S., Sharma, P., Kumar, S., Sinhmar, Y., Kumar, D., Sharma, A., 2022. Silicon supplementation alleviates the salinity stress in wheat plants by enhancing the plant water status, photosynthetic pigments, proline content and antioxidant enzyme activities. Plants. 11, 2525. https://doi.org/10.3390/plants11192525
Soltanieh, M., Talei, D., Nejatkhah, P., 2023. Performance reaction and biochemical properties of black cumin under the influence of different regimes of nitrogen, methanol and deought stress. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 71, 15-33.
Sorkhi, F., 2021. Effect of vermicompost fertilizer on antioxidant enzymes and chlorophyll contents in Borago officinalis under salinity stress. Iranian Journal of Plant Physiology. 11, 3589-3598. [In Persian with English summary].
Stewart, R.R., Bewley, J.D., 1980. Lipid peroxidation associated with accelerated aging of soybean axes. Plant Physiology. 65, 245-248. https://doi.org/10.1104/pp.65.2.245
Sudhakar, C., Lakshmi, A., Giridarakumar, S., 2001. Changes in the antioxidant enzyme efficacy in two high yielding genotypes of mulberry (Morus alba L.) under NaCl salinity. Plant Science. 161, 613-619. https://doi.org/10.1016/S0168-9452(01)00450-2
Tammam, A.A., Shehata, M.R.A.M., Pessarakli, M., El-Aggan, W.H., 2022. Vermicompost and its role in alleviation of salt tress in plants–II. Impact of vermicompost on the physiological responses of salt-stressed plants. Journal of Plant Nutrition. 45, 1-21. https://doi.org/10.1007/s11738-022-03481-9
Vahabzadeh, M., Ghodsi, M., Vahabzadeh, M., Nazeri, M., Khodarahmi, M., Ghasemi, M., Kohkan, S.A. Tajalli, H., 2013. Sanabad, A new triticale cultivar suitable for temperate and cold-temperate regions of Iran. Research Achievements for Field and Horticulture Crops. 2, 143-153 [In Persian with English summary].
Valizadeh-Kamran, R., Vojodi Mehrabani, L., Pessarakli, M., 2019. Effects of foliar application of methanol on some physiological characteristics of Lavandula stoechas L. under NaCl salinity conditions. Journal of Plant Nutrition. 42, 261-268. https://doi.org/10.1080/01904167.2018.1554677
Vojodi Mehrabani, L., 2019. The effects of methanol and ethanol foliar application under salinity stress on some physiological characteristics of Pelargonium graveolens L. Journal of Plant Physiology and Breeding. 9, 63-73.
Yasin, N.A., Akram, W., Khan, W.U., Ahmad, S.R., Ahmad, A., Ali, A., 2018. Halotolerant plant-growth promoting rhizobacteria modulate gene expression and osmolyte production to improve salinity tolerance and growth in Capsicum annum L. Environmental Science and Pollution Research, 25, 23236-23250. https://doi.org/10.1007/s11356-018-2381-8
Yousefi, K., Jamei, R., Darvishzadeh, R., 2022. Study of some physiological and biochemical parameters of Okra plant (Abelmoschus esculentus L.) under salt stress in the presence of chitosan nanoparticles. Iranian Journal of Plant Biology. 14, 17-38. [In Persian with English summary].
Zhao, Y., Zeng, Z.D., Qi, C.J., Yu, X.S., Guo, C.L., Chen, Q., Chen, L.M., 2014. Deciphering the molecular responses to methanol-enhanced photosynthesis and stomatal conductance in broad bean. Acta Physiologiae Plantarum. 36, 2883-2896. https://doi.org/10.1007/s11738-014-1658-x
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