نوع مقاله : مقاله پژوهشی

نویسندگان

گروه آگروتکنولوژی دانشکده کشاورزی دانشگاه تربیت مدرس

چکیده

به‌منظور ارزیابی تأثیر تنش کم ­آبی بر صفات فیزیولوژیکی و کیفیت علوفه شبدر ایرانی (Trifolium resupinatum L.) آزمایشی طی سال‌های زراعی 1396 و 1397 به‌صورت طرح بلوک‏های کامل تصادفی در مزرعه تحقیقاتی دانشگاه تربیت مدرس به اجرا درآمد. تیمارهای مورد آزمایش شامل تنش کم­آبی در چهار سطح (آبیاری پس از رسیدن به سطوح 80، 60، 40 و 20 درصد رطوبت قابل‌دسترس) بود. آبیاری تمامی تیمارها تا زمان شروع تنش در مراحل پایانی رشد رویشی چین دوم در سطح 70 درصد آب در دسترس انجام شد. نتایج نشان داد تنش کم­آبی بر تمامی صفات­ موردبررسی اثر معنی­داری داشت. تیمارهای آبیاری در 80 و 60 درصد آب در دسترس گیاه، با عملکرد ماده خشک 5.10 و 4.98 تن در هکتار بالاترین مقدار را داشتند و تعویق آبیاری تا حدود 40 و 20 درصد آب در دسترس گیاه موجب کاهش معنی­ دار 19 و 36 درصدی این صفت شد. همچنین آبیاری در سطح 20 درصد موجب کاهش 27 درصدی محتوی پروتئین علوفه، افزایش حدود 12 درصدی الیاف نامحلول در شوینده­ های اسیدی و خنثی و درنهایت کاهش 16 درصدی ارزش غذایی نسبی شد. در مقایسه روند تغییرات صفات فیزیولوژیک در 10 روز ابتدایی شروع تنش ­آبی، افزایش پیوسته دمای برگ از 30.5 تا 34 درجه سانتی­گراد، افت 49، 50 و 15 درصدی سرعت فتوسنتز، هدایت روزنه­ ای و شاخص سبزینگی و کاهش غلظت کلروفیل a و b به میزان 25 و 40 درصد مشاهده ­شد. هرچند کاهش 80 درصدی رطوبت در دسترس، سبب کاهش هدایت روزنه‌ای و افزایش دمای برگ شده بود، اما با برگشت به شرایط نرمال رطوبتی، دمای برگ و هدایت روزنه‌ای با سرعت و سایر صفات به‌آرامی به وضعیت پیش از تنش بازگشتند. با توجه به همبستگی بالا بین سرعت فتوسنتز و عملکرد علوفه توجه بیشتر به این صفت در پژوهش­های آینده لازم به نظر می­رسد. درمجموع نتایج نشان ­داد آبیاری در سطح 60 درصد ظرفیت زراعی هم می­تواند عملکرد کیفی و کمی بالا و قابل قبولی داشته باشد و هم در مصرف آب بهینه عمل کرد.

کلیدواژه‌ها

موضوعات

 Aaltonen, H., Lindén, A., Heinonsalo, J., Biasi, C., Pumpanen, J., 2017. Effects of prolonged drought stress on Scots pine seedling carbon allocation. Tree Physiology. 37, 418-427. https://doi.org/10.1093/treephys/tpw119   
Abbasi, M., Hassanzadeh, A., Mahdipour, A., Anahid, S. and Safari, S., 2019. Forage yield in some Iranian wild Trifolium genetic resources under different climatic and irrigation conditions. Journal of Agricultural Science and Technology. 21, 993- 1004. https://jast.modares.ac.ir/article-23-16639-en.html
Abbasi, M., Mokhtarpour, H., Zamaniyan, M., Mahdipour, A. 2022. Investigation of forage production in some cultivated and wild annual clovers using green water in semi-humid temperate regions of Iran', Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research. 30, 133-144. [In Persian]. https://doi.org/10.22092/ijrfpbgr.2022.358581.1417  
Addington, R.N., Mitchell, R.J., Oren, R., Donovan, L.A., 2004. Stomatal sensitivity to vapor pressure deficit and its relationship to hydraulic conductance in Pinus palustris. Tree Physiology. 24, 561–569. https://doi.org/10.1093/treephys/24.5.561
Arnon, A.N., 1967. Method of extraction of chlorophyll in the plants. Agronomy Journal. 23, 112-121.
Balazadeh, M., Zamanian, M., Golzardi., F., Torkashvand, A. M., 2021. Effects of Limited Irrigation on Forage Yield, Nutritive Value and Water Use Efficiency of Persian Clover (Trifolium Resupinatum) Compared to Berseem Clover (Trifolium alexandrinum). Communications in Soil Science and Plant Analysis. 52, 1927-1942. https://doi.org/10.1080/00103624.2021.1900228 
Berrada, A., 2005. Alfalfa response to water deficit using subsurface drip irrigation. Colorado State University. Agricultural experiment station. Technical Bulletin TB 05 – 01 Colorado State University., Ft. Collins, Co
Bingol, N.T., Karsli, M.A., Yilmaz I.H., Bolat D., 2007. The effects of planting time and combination on the nutrient composition and digestible dry matter yield of four mixtures of vetch varieties intercropped with barely. The Turkish Journal of Veterinary and Animal Sciences. 31, 297-302.
Carmi, A., Aharoni, Y., Edelstein, M., Umiel, N., Hagiladi, A., Yosef, E., Nikbachat, M., Zenou, A., Miron, J., 2006. Effects of irrigation and plant density on yield, composition and in vitro digestibility of a new forage sorghum variety, Tal, at two maturity stages. Animal Feed Science and Technology. 131, 120-132.
Damour, G., Vandame, M., Urban, L., 2008. Long-term drought modifies the fundamental relationships between light exposure, leaf nitrogen content and photosynthetic capacity in leaves of the lychee tree (Litchi chinensis). Journal of Plant Physiology. 165, 1370-8. https://doi.org/10.1016/j.jplph.2007.10.014
Demirkol, G., 2021. PopW enhances drought stress tolerance of alfalfa via activating antioxidative enzymes, endogenous hormones, drought related genes and inhibiting senescence genes. Plant Physiology and Biochemistry. 166, 540-548. https://doi.org/10.1016/j.plaphy.2021.06.036
Ebrahimi-Sborezi, H., Modarres-Sanavy, S.A.M., Baghbani-Arani, A., 2021. Assessment of morpho-physiological and quantitative and qualitative yield of Peppermint (Mentha piperita L.) under different irrigation regimes and application of different nitrogen fertilizer. Environmental Stress in Crop Science. 14, 425-437. [In Persian]. https://doi.org/10.22077/escs.2019.2893.1744
Egan, L., Hofmann, R., Nichols, S., Hadipurnomo, J., Hoyos-Villegas, V., 2021. Transpiration Rate of White Clover (Trifolium repens L.) Cultivars in Drying Soil. Frontiers in Plant Science. 12, 1-14. https://doi.org/10.3389/fpls.2021.595030
Ghaderi, S., Ghorbani, J., Gholami, P., Karimzadeh, A., Salarianf, F., 2011. Effect of drought and salinity stresses on germination indices of vetch (Vida illosa L). Journal of Agroecology, 3, 120-129. [In Persian]. https://doi.org/10.22067/JAG.V3I1.9977
Ghaffari, A., Akram, N.A., Ashraf, M., Ashrif, Y., Aadiq, M., 2019. Thiamin-induced variations in oxidative defense processes in white clover (Trifolium repens L.) under water deficit stress,. Turkish Journal of Botany. 43, 5. https://doi.org/10.3906/bot-1710-34
Grassi, G., Magnani, F., 2005. Stomatal, mesophyll conductance and biochemical limitations to photosynthesis as affected by drought and leaf ontogeny in ash and oak trees. Plant, Cell & Environment. 28, 834–49. https://doi.org/10.1111/j.1365-3040.2005.01333.x
Grev, A.M., Wells, M.S., Samac, D.A., Martinson, K.L., Sheaffer, C.C., 2017. Forage Accumulation and Nutritive Value of Reduced Lignin and Reference Alfalfa Cultivars. Agronomy Journal. 109, 2749-2761. https://doi.org/10.2134/agronj2017.04.0237
Heidarzadeh, A., Modarres-Sanavy, S.A.M., Mokhtassi-Bidgoli, A. 2021. Investigate different nutritional regimens on some forage quantitative and qualitative traits of Dracocephalum kotschyi Boiss under water deficit stress. Iranian Journal of Field Crop Science. 52, 15-27. [In Persian]. https://doi.org/10.22059/IJFCS. 2020.292093.654654
Heidarzadeh, A., Modarres-Sanavy, S.A.M., Mokhtassi-Bidgoli, A., 2022. Changes in Yield and Essential Oil Compositions of Dracocephalum kotschyi Boiss in Response to Azocompost, Vermicompost, Nitroxin, and Urea Under Water Deficit Stress. Journal of Soil Science and Plant Nutrition. 22, 896–913. https://doi.org/10.1007/s42729-021-00700-z
Hu, Y., Kang, S., Ding, R., Zhao, Q., 2021. A crude protein and fiber model of alfalfa incorporating growth age under water and salt stress. Agricultural Water Management. 255, 107037. https://doi.org/10.1016/j.agwat.2021.107037
Jafari, A.A., Connolly, V., Frolich, A., Walsh, E.K., 2003. A note on estimation of quality in perennial Ryegrass by Near Infrared Spectroscopy. Irish Journal of Agricultural and Food Research. 42, 293-299.
Jahanzad, E., Jorat, M., Moghadam, H., Sadeghpour, A., Chaichi, M.R., Dashtaki, M., 2013. Response of a new and a commonly grown forage sorghum cultivar to limited irrigation and planting density. Agricultural Water Management. 117, 62-69. https://doi.org/10.1016/j.agwat.2012.11.001
Jungers, J. Cherney, J., Martinson, K. Jaqueth, A. Sheaffer, C., 2020. Forage nutritive value of modern alfalfa cultivars. Crop Forage Turfgrass Manag. 6, 20076. https://doi.org/10.1002/cft2.20076  
Karimi, H., 1996. Plant Physiology. University of Tehran Press. pp. 105-114. [In persian].
Lange, O.L., Lösch, R., Schulze, E.D., Kappen, L., 1971. Responses of stomata to changes in humidity. Planta. 100, 76–86. https://doi.org/10.1007/BF00386887
Lithourgidis, A.S., Vaslakoglou, I.B., Dhima, K.V., Dordas, C.A., Yiakoulaki, M. D., 2006. Forage yield and quality of common vetch mixtures with oat and triticale in two seeding ratios. Field Crops Reserch. 99, 106-113. https://doi.org/10.1016/j.fcr.2006.03.008
Lösch, R., 1979. Stomatal responses to changes in air humidity. In: Sen, D.N. (Ed.), Structure, Function and Ecology of Stomata. Bishen Singh Mahendra Pal Singh, Dehra Dunn, India. 189–216.
Ma, Q., Behboudian, M.H., Turner, N.C., Palta, J.A., 2001. Gas exchange by pods and subtending leaves and internal recycling of CO2 by pods of chickpea (Cicer arietinum L.) subjected to water deficits. Journal of Experimental Botany. 52, 123–131. https://doi.org/10.1093/jexbot/52.354.123
MacFarlane, C., White, D.A., Adams, M.A., 2004. The apparent feedforward response to vapour pressure deficit of stomata in droughted, field-grown Eucalyptus globulus Labill. Plant, Cell & Environment, 27, 1268–80. https://doi.org/10.1111/j.1365-3040.2004.01234.x
Markovi´c, J., Petrovi´c, M., Terzi´c, D., Vasi´c, T., Kosti´c, I., Štrbanovi´c, R., Grubi´c, G., 2019. Protein fractions as influenced by cultivars, stage of maturity and cutting dates in alfalfa (Medicago sativa L). Legume Research. 1, 46-51. https://doi.org/10.18805/LR-479
McDonald, P., Edwards, R. A., Greenhalgh, J. F. D., Morgan, C. A., Sinclair, L. A., Wilkinson, R. G., 2011. Animal Nutrition (7thed). Person Education limited, 685 Pp.
Minguo, L., Zikui, W., Le, M., Rui, X., Huimin,.,2021. Effect of regulated deficit irrigation on alfalfa performance under two irrigation systems in the inland arid area of midwestern China. Agricultural Water Management. (248) 106764. https://doi.org/10.1016/j.agwat.2021.106764
Naveed, M., Ditta, A., Ahmad, M., Mustafa, A., Ahmad, Z., Conde-Cid, M., Tahir, S., Shah, S.A.A., Abrar, M.M., Fahad, S., 2021. Processed animal manure improves morphophysiological and biochemical characteristic of Brassica napus L. under nickel and salinity stress. Environmental Science and Pollution Research. 385, 114816. https://doi.org/10.1007/s11356-021-14004-3
Nematollahi, D., Eisvand, H. R., Modaerrs Sanavy, S. A. M., Akbari, N., Ismaili, A., 2020. Effects of low irrigation on yield quantity and quality of clover species under high input management conditions', Iranian Journal of Field Crop Science, 51, pp. 47-57. [In Persian]. https://doi.org/10. 22059/ijfcs.2019.251696.654444  
Nikou, Sh., Pouryousef Miandoab., M., Hassanzadeh Gorttape, A., 2014. Evaluation of annual clover ecotypes by using drought tolerance indices. Journal of Crop Ecophysiology. 8, 375-393. [In Persian].
O’Brien, M.J., Philipson, C.D., Tay, J., Hector, A. 2013. The influence of variable rainfall frequency on germination and early growth of shade-tolerant dipterocarp seedlings in Borneo. PLoS ONE. 8, e70287. https://doi.org/10.1371/journal.pone.0070287
Oue, H., Mon, M.M., Irsyad, F., Utami, A.S., Zaw, Y., 2021. Stomatal and photosynthetic responses of soybean with two types of mulch under different soil water conditions. IOP Conference Series: Earth and Environmental Science, Volume 1059, The 4th International Conference on Sustainability Agriculture and Biosystem. Online. https://doi.org/10.1088/1755-1315/1059/1/012038
Pariyar, S., Eichert, T., Goldbach, E.H., Hunsche, M., Burkhardt, J., 2013. The exclusion of ambient aerosols changes the water relations of sunflower (Helianthus annuus) and bean (Vicia faba) plants, Environmental and Experimental Botany. 88, 43-52. https://doi.org/10.1016/j.envexpbot.2011.12.031
Rohweder, D.A., Barnes, R.F., Jorgensen, N., 1978. Proposed hay grading standards based on laboratory analyses for evaluating quality. Journal of Animal Sciences. 47, 747–759.
Rouphael, Y, Cardarelli, M., Schwarz, D., Franken, P., Colla, G., 2012. Effects of drought on nutrient uptake and assimilation in vegetable crops. In: Aroca R (ed) Plant responses to drought stress. Springer, Berlin, 171-195. https://doi.org/10.1007/978-3-642-32653-0_7
Sadeghi, H., Khani, K., 2012. Effects of different drought and salinity stress levels on some morphological characteristics and proline content of annual burr medics (Medicago polymorpha L.). Iranian Dryland Agronomy Journal. 1, 1-13. [In Persian]. https://doi.org/10.22059/IJHS.2018.264753.1507
Spinedi, N., Storb, R., Aranda, E., Romani, F., Svriz, M., Varela, S.A., Moreno, J.E., Fracchia, S., Cabrera, J., Batista-García, R.A., Ponce de León, I., Scervino, J.M., 2021. ROS-Scavenging Enzymes as an Antioxidant Response to High Concentration of Anthracene in the Liverwort Marchantia polymorpha L. Plants. 10, 1478. https://doi.org/10.3390/plants10071478
Staniak, M., 2019. Changes in yield and nutritive value of red clover (Trifolium pratense L.) and Festulolium (Festulolium braunii (K. Richt) A. Camus) under drought stress. Agricultural and Food Science. 28, 27–34. https://doi.org/10.23986/afsci.73282
Wróbel, B., Zielewicz, W., 2019. Nutritional value of red clover (Trifolium pratense L.) and birdsfoot trefoil (Lotus corniculatus L.) harvested in different maturity stages. Journal of Research and Applications in Agricultural Engineering. 64, 14-19.
Zamanian, M., 2005. The effect of planting season on forage production of clover species. Seed and Plant. 21, 159-173. [In Persian].
Zeiditoolabi, N., Direkvandy, S., Rahmati, T., Vlizadeh, S., Dolatshah, A., 2015. Comparison of quantitative and qualitative traits of different forage vetch species under rain-fed and supplemental irrigation conditions. Journal of Agronomy Sciences. 5, 13-26.