نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ارشد زراعت، گروه تولید و ژنتیک گیاهی، دانشکده علوم و مهندسی کشاورزی، پردیس کشاورزی و منابع طبیعی، دانشگاه رازی، کرمانشاه

2 دانشیار، گروه تولید و ژنتیک گیاهی، دانشکده علوم و مهندسی کشاورزی، پردیس کشاورزی و منابع طبیعی، دانشگاه رازی، کرمانشاه

3 استاد، گروه مهندسی آب، دانشکده کشاورزی، پردیس کشاورزی و منابع طبیعی، دانشگاه رازی، کرمانشاه

چکیده

استویا گیاهی چند­ساله با برگ­ های شیرین که حدود 300 بار شیرین­تر از ساکارز (شکر) بوده و امروزه در نقاط زیادی در دنیا کشت می­گردد. تنش خشکی و کمبود نیتروژن از مهم­ترین تنش­های محیطی محدودکننده تولید این گیاه بوده و اثرات نامطلوبی بر رشد و نمو گیاه و سایر فرآیند­های متابولیکی دارد. ازاین‌رو، به‌منظور بررسی اثر تنش خشکی و کود نیتروژن بر رشد و عملکرد استویا، آزمایشی به‌صورت فاکتوریل و در قالب طرح کاملاً تصادفی در سه تکرار در پردیس کشاورزی و منابع طبیعی، دانشگاه رازی، در سال 1395 اجرا گردید. فاکتورها شامل تنش خشکی (بدون تنش، تنش ملایم و تنش شدید) و کود نیتروژن (0، 50، 100، 150 و 200 کیلوگرم در هکتار) بودند. زمان آبیاری برای بدون تنش، تنش ملایم و شدید به ترتیب 50، 65 و 80 درصد تخلیه رطوبت قابل‌استفاده در خاک بودند. نتایج نشان داد که تنش خشکی و میزان نیتروژن بر صفات زیست‌توده، ماده خشک برگ، ماده خشک ساقه، کلروفیل­های a، b و کارتنوئید­ها، محتوای نسبی آب برگ، کارایی مصرف آب، قند­های محلول، پرولین و آنزیم­ های سوپراکسید دیسموتاز، پراکسیداز و کاتالاز اثر معنی­ داری داشتند. بیشترین ماده خشک برگ در تیمار بدون تنش خشکی و مصرف 200 کیلوگرم نیتروژن در هکتار در چین اول و چین دوم به ترتیب 20.4 و 17.1 گرم در بوته و کمترین مقدار ماده خشک برگ در تیمار تنش شدید و مصرف 200 کیلوگرم نیتروژن در چین اول و دوم به ترتیب 6.81 و 5.86 گرم در بوته به دست آمد. به‌طورکلی نتایج نشان داد که تولید برگ استویا در شرایط آب و هوایی کرمانشاه با مصرف آب زیاد امکان‌پذیر بوده و در شرایط تنش خشکی در چین اول و چین دوم به ترتیب 66.6 و 66.3 درصد نسبت به شاهد کاهش نشان دادند.

کلیدواژه‌ها

موضوعات

 Abdullateef, R.A., Osman, M., 2012. Studies on effects of pruning on vegetative traits in Stevia rebaudiana Bertoni (Compositae). International Journal of Biology. 4, 146-153. https://dx.doi.org/10.5539/ijb.v4n1p146
Abou-Arab, A.E., Abou-Arab, A.A., Abu-Salem, M.F., 2010. Physico-chemical assessment of natural sweeteners steviosides produced from Stevia rebaudiana Bertoni plant. African Journal of Food Science. 4, 269-281. https://dx.doi.org/10.5897/AJFS.9000226
Akbar Khalil, S., Zamir, R., Ahmad, N., 2014. Selection of suitable propagation method for consistent plantlets production in Stevia rebaudiana (Bertoni). Saudi Journal of Biological Sciences. 21, 566-573. https://dx.doi.org/10.1016/j.sjbs.2014.02.005
Amini, S., Ghobadi, C., Yamchi, A., 2015. Proline accumulation and osmotic stress: an overview of P5CS gene in plants. Journal of Plant Molecular Breeding. 3, 44-55. https://dx.doi.org/10.22058/jpmb.2015.17022
Anbazhagan, M., Kalpana, M., Rajendran, R., Natarajan, V., Dhanavel, D., 2010. In vitro production of Stevia rebaudiana Bertoni. Emirates Journal of Food and Agriculture. 216-222. https://dx.doi.org/10.9755/ejfa.v22i3.4891
Anjum, S.A., Farooq, M., Xie, X.Y., Liu, X.J., Ijaz, M.F., 2012. Antioxidant defense system and proline accumulation enables hot pepper to perform better under drought. Scientia Horticulturae. 140, 66-73. https://dx.doi.org/10.1016/j.scienta.2012.03.028
Aranjuelo, I., Molero, G., Erice, G., Christophe Avice, J., Nogues, S., 2011. Plant physiology and proteomics reveals the leaf response to drought in alfalfa (Medicago sativa L.). The Journal of Experimental Botany. 62, 111-123. https://dx.doi.org/10.1093/jxb/erq249
Armand, N., Amiri, H., Ismaili, A., 2016. Interaction of methanol spray and water‐deficit stress on photosynthesis and biochemical characteristics of Phaseolus vulgaris L. cv. Sadry. Photochemistry and Photobiology. 92, 102-110. https://dx.doi.org/10.1111/php.12548
Arnon, A.N., 1967. Method of extraction of chlorophyll in the plants. Agronomy Journal. 23, 112-121.
Atteya, A.M., 2003. Alteration of water relations and yield of corn genotypes in response to drought stress. Bulgarian Journal of Plant Physiology. 29, 63-76.
Barr, H.D., Weatherley, P.E., 1962. A re-examination of the relative turgidity technique for estimating water deficit in leaves. Australian Journal of Biological Sciences. 15, 413-428. https://dx.doi.org/10.1071/BI9620413
Bates, L.S., Waldern, R.P., Tear, I.D., 1973. Rapid determination of free proline for water- stress studies. Plant and Soil. 39, 205-207. https://dx.doi.org/10.1007/BF00018060
Beauchamp, C., Fridovich, I., 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry. 44, 276-287. https://dx.doi.org/10.1016/0003-2697(71)90370-8
Bindi, M., Hacour, A., Vandermeiren, K., Craigon, J., Ojanperä, K., Selldén, G., Hogy, P., Fibbi, L., 2002. Chlorophyll concentration of potatoes grown under elevated carbon dioxide and/or ozone concentrations. European Journal of Agronomy. 17, 319-335. https://dx.doi.org/10.1016/S1161-0301(02)00069-2
Ceunen, S., Geuns, J.M., 2013. Steviol glycosides: Chemical diversity, metabolism, and function. Journal of Natural Products. 76, 1201-1228. https://dx.doi.org/10.1021/np400203b
Chaturvedula, V.S., Rhea, J., Milanowski, D., Mocek, U., Prakash, I., 2011. Two minor diterpene glycosides from the leaves of Stevia rebaudiana. Natural Product Communications. 6, 175-178. https://dx.doi.org/10.1177/1934578X1100600205  
Chrysargyris, A., Laoutari, S., Litskas, V.D., Stavrinides, M.C., Tzortzakis, N., 2016. Effects of water stress on lavender and sage biomass production, essential oil composition and biocidal properties against Tetranychus urticae (Koch). Scietia Horticulture. 213, 96-103. https://dx.doi.org/10.1016/j.scienta.2016.10.024
Daniels, L., Hanson, R., Philips, J., Gerhardt, P., Murray, R.G.E., Wood, W., Krieg, N.R., 1994. Chemical Analysis Methods for General and Molecular Bacteriology. Washington, DC: American Society of Microbiology. Chap. 22.
Durán, A.S., Rodríguez, N.M.P., Cordón, A.K., Record, C.J., 2013. Stevia (Stevia rebaudiana), non-caloric natural sweetener. Revista Chilena de Nutrición. 39, 203–206. https://dx.doi.org/10.4067/S0717-75182012000400015
El-Wahab, A., Mohamed, A., 2007. Effect of nitrogen and magnesium fertilization on the production of Trachyspermum ammi L. (Ajowan) plants under Sinai conditions. Journal of Applied Sciences Research. 3, 781-786.
Farooq, M., Wahid, A., Kobayashi, N., Fujita, D., Basra, S.M.A., 2009. Plant drought stress: effects, mechanisms and management. Agronomy for Sustainable Development. 29, 185–212. https://dx.doi.org/10.1007/978-90-481-2666-8_12
Gardner, F.P., Pearce R.B., Mitchell, R.L., 1984. Physiology of Crop Plants. 1st Edition. ‎Iowa State Press. 327p.
Ghaffari, G., Toorchi, M., Aharizad, S., Shakiba, M. R., 2011. Evaluation of traits related to water deficit stress in winter Rapeseed cultivars. Universal Journal of Environmental Research and Technology. 1, 338-350.
Ghorbanli, M., Gafarabad, M., Amirkian, T., Allahverdi Mamaghani, B., 2013. Investigation of proline, total protein, chlorophyll, ascorbate and dehydroascorbate changes under drought stress in Akria and Mobil tomato cultivars. Iranian Journal of Plant Physiology. 3, 651-658. [In Persian]
Gonzalez-Dugo, V., Durand, J. L., Gastal, F., 2010. Water deficit and nitrogen nutrition of crops. A review. Agronomy for Sustainable Development. 30, 529-544. https://dx.doi.org/10.1051/agro/2009059
Hajihashemi, S., Ehsanpour, A., 2013. Influence of exogenously applied paclobutrazol on some physiological traits and growth of Stevia rebaudiana under in vitro drought stress. Biologia. 68, 414-420. https://dx.doi.org/10.2478/s11756-013-0165-7
Hasheminasab, H., Assad, M.T., Aliakbari, A., Sahhafi, S.R., 2012. Influence of drought stress on oxidative damage and antioxidant defense systems in tolerant and susceptible wheat genotypes. Journal of Agricultural Science. 4, 20-30. [In Persian]
Hassegawa, R.H., Fonseca, H., Fancelli, A.L., da Silva, V.N., Schammass, E.A., Reis, T.A., Corrêa, B., 2008. Influence of macro-and micronutrient fertilization on fungal contamination and fumonisin production in corn grains. Food Control. 19, 36-43. https://dx.doi.org/10.1016/j.foodcont.2007.01.006
Huseynova, I.M., 2012. Photosynthetic characteristics and enzymatic antioxidant capacity of leaves from wheat cultivars exposed to drought. Biochimica et Biophysica Acta (BBA)-Bioenergetics. 1817, 1516-1523. https://dx.doi.org/10.1016/j.bbabio.2012.02.037
Kumar, R., Sharma, S., Ramesh, K., Singh, B., 2013. Effects of shade regimes and planting geometry on growth, yield and quality of the natural sweetener plant stevia (Stevia rebaudiana Bertoni) in north-western Himalaya. Archives of Agronomy and Soil Science. 59, 963-979. https://dx.doi.org/10.1080/03650340.2012.699676
Lawlor, D.W., Cornic, G., 2002. Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants. Plant, Cell and Environment. 25, 275-294. https://dx.doi.org/10.1046/j.0016-8025.2001.00814.x
Muanda, F.N., Soulimani, R., Diop, B., Dicko, A., 2011. Study on chemical composition and biological activities of essential oil and extracts from Stevia rebaudiana Bertoni leaves. LWT - Food Science and Technology. 44, 1865– 1872. https://dx.doi.org/10.1016/j.lwt.2010.12.002
Naureen, G., Naqvi, F.N., 2010. Salt tolerance classification in wheat genotypes using reducing sugar accumulation and growth characteristics. Emirates Journal of Food and Agriculture. 22, 308-317. https://dx.doi.org/10.9755/ejfa.v22i4.4878
Odlare, M., Pell, M., Svensson, K., 2008. Changes in soil chemical and microbiological properties during 4 years of application of various organic residues. Waste Management. 28, 1246-1253. https://dx.doi.org/10.1016/j.wasman.2007.06.005 
Pal, P.K., Kumar, R., Guleria, V., Mahajan, M., Prasad, R., Pathania, V., Singh, R.D., 2015. Crop-ecology and nutritional variability influence growth and secondary metabolites of Stevia rebaudiana Bertoni. BMC Plant Biology. 15, 1-16. https://dx.doi.org/10.1186/s12870-015-0457-x
Parida, A.K., Dagaonkar, V.S., Phalak, M.S., Aurangabadkar, L.P., 2008. Differential responses of the enzymes involved in proline biosynthesis and degradation in drought tolerant and sensitive cotton genotypes during drought stress and recovery. Acta Physiologiae Plantarum. 30, 619-627. https://dx.doi.org/10.1007/s11738-008-0157-3
Pierret, A., Moran, C.J., Doussan, C., 2005. Conventional detection methodology is limiting our ability to understand the roles and functions of fine roots. New Phytologist. 166, 967-980. https://dx.doi.org/10.1111/j.1469-8137.2005.01389.x
Porporato, A., D’odorico, P., Laio, F., Rodriguez-Iturbe, I., 2003. Hydrologic controls on soil carbon and nitrogen cycles. I. Modeling scheme. Advances in Water Resources. 26, 45-58. https://dx.doi.org/10.1016/S0309-1708(02)00094-5
Ramesh, K., Singh, V., Megeji, N.W., 2006. Cultivation of Stevia [Stevia rebaudiana (Bert.) Bertoni]: A comprehensive review. Advances in Agronomy. 89, 137-177. https://dx.doi.org/10.1016/S0065-2113(05)89003-0
Reis, M., Coelho, L., Santos, G., Kienle, U., Beltrão, J., 2015. Yield response of stevia (Stevia rebaudiana Bertoni) to the salinity of irrigation water. Agricultural Water Management. 152, 217-221. https://dx.doi.org/10.1016/j.agwat.2015.01.017
Rivelli, A.R., De Maria, S., Pizza, S., Gherbin, P., 2010. Growth and physiological response of hydroponically-grown sunflower as affected by salinity and magnesium levels. Journal of Plant Nutrition. 33, 1307-1323. https://dx.doi.org/10.1080/01904167.2010.484092
Sairkar, P., Shukla, N.P., Mehrotra, N.N., 2009. Mass production of an economically important medicinal plant Stevia rebaudiana using in vitro propagation techniques. Journal of Medicinal Plants Research. 3, 266-270. https://dx.doi.org/10.5897/JMPR.9000862
Saxton, K.E., Rawls, W.J., 2006. Soil water characteristic estimates by texture and organic matter for hydrologic solutions. Soil Science Society of America Journal. 70, 1569–1578. https://dx.doi.org/10.2136/sssaj2005.0117
Setter, T.L., Flannigan, B.A., Melkonian, J., 2001. Loss of kernel set due to water deficit and shade in maize. Crop Science. 41, 1530-1540. https://dx.doi.org/10.2135/cropsci2001.4151530x 
Sinha, A.K., 1972. Colorimetric assay of catalase. Analytical biochemistry. 47, 389-394. https://dx.doi.org/10.1016/0003-2697(72)90132-7
Taiz, L. Zeiger, E., Moller, I.M., Murphy, A. 2015. Plant Physiology and Development. 6th Edition, Sinauer Associates, Sunderland, CT. p: 761.
Turtola, S., Manninen, A.M., Rikala, R., Kainulainen, P., 2003. Drought stress alters the concentration of wood terpenoids in Scots pine and Norway spruce seedlings. Journal of Chemical Ecology. 29, 1981-1995. https://dx.doi.org/10.1023/A:1025674116183
Vasilakoglou, I., Kalfountzos, D., Gougoulias, N., Reppas, C., 2016. Productivity of two stevia varieties under reduced irrigation and fertilization inputs. Archives of Agronomy and Soil Science. 62, 457-472. https://dx.doi.org/10.1080/03650340.2015.1060554
Woelwer-Rieck, U., Lankes, C., Wawrzun, A., Wüst, M., 2010. Improved HPLC method for the evaluation of the major steviol glycosides in leaves of Stevia rebaudiana. European Food Research and Technology. 231, 581-588. https://dx.doi.org/10.1007/s00217-010-1309-4
Wu, F., Bao, W., Li, F., Wu, N., 2008. Effects of drought stress and N supply on the growth, biomass partitioning and water-use efficiency of Sophora davidii seedlings. Environmental and Experimental Botany. 63, 248-255. https://dx.doi.org/10.1016/j.envexpbot.2007.11.002
Yadav, A.K., Singh, S., Dhyani, D., Ahuja, P.S., 2011. A review on the improvement of stevia [Stevia rebaudiana (Bertoni)]. Canadian Journal of Plant Science. 91, 1-27. https://dx.doi.org/10.4141/cjps10086
Yadav, R.S., Bhushan, C., 2001. Effect of moisture stress on growth and yield in rice genotypes. Indian Journal of Agricultural Research. 35, 104-107.
Yousfi, S., Márquez, A.J., Betti, M., Araus, J.L., Serret, M.D., 2016. Gene expression and physiological responses to salinity and water stress of contrasting durum wheat genotypes. Journal of Integrative Plant Biology. 58, 48-66. https://dx.doi.org/10.1111/jipb.12359