نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه زراعت، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران

2 موسسه تحقیقات اصلاح و تهیه بذر چغندرقند، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

چکیده

به منظور بررسی تأثیر مصرف پاکلوبوترازول و باکتری‌های محرک رشد در سطوح مختلف آبیاری بر تغییرات صفات زراعی گندم آزمایشی به صورت فاکتوریل اسپلیت پلات در قالب طرح پایه بلوک‌های کامل تصادفی با سه تکرار در دو منطقه قم و همدان در سال زراعی 1398-1397 انجام شد. فاکتور‌های آزمایش شامل میزان آبیاری با سه سطح (آبیاری در 40 درصد تخلیه رطوبتی قابل دسترس در تمام دوره رشد-ی(نرمال)، آبیاری نرمال تا گرده‌افشانی و آبیاری در 60 درصد تخلیه رطوبت قابل دسترس تا پایان دوره رشد‌ی، آبیاری در 40 درصد تخلیه رطوبت قابل دسترس تا گرده‌افشانی و قطع آبیاری تا پایان دوره رشدی) ، محلول پاشی پاکلوبوترازول با سه سطح (محلول‌پاشی با آب خالص، 50 و 100 پی پی ام در دو مرحله ساقه‌‌دهی و خوشه‌‌دهی اعمال شد) و باکتری‌های محرک رشد با پنج سطح(عدم مصرف، مایکوباکتریوم، ازتوباکتر، آزوسپیریلوم و تلفیق سه باکتری) بودند. نتایج نشان دادند اثر متقابل رژیم آبیاری در پاکلوبوترازول برای عملکرد بیولوژیک و عملکرد دانه معنی دار شد به صورتی که درسطح آبیاری سوم، استفاده از پاکلوبوترازول با غلظت 100 پی‌پی‌ام باعث افزایش عملکرد دانه و عملکرد بیولوژیک شد. همچنین بر اساس جدول تجزیه واریانس داده‌ها اثر متقابل رژیم آبیاری در باکتری برای عملکرد بیولوژیک و عملکرد دانه معنی دار شد به طوری که در سطح سوم رژیم آبیاری دو سطح تلفیق سه باکتری و ازتوباکتر به ترتیب با 19.05 و 17.95 تن در هکتار بیشترین عملکرد بیولوژیک را داشتند.

کلیدواژه‌ها

موضوعات

Abbasi, A.R., Shafi Rahmani, M., Vafaei, Y., 2000. Plant Biochemistry. Translation. First Edition. University of Tehran Press. 232-233. [In Persian].
Ahmadian, K., Jalilian, J., Pirzad, A., 2021. Nano-fertilizers improved drought tolerance in wheat under deficit irrigation. Journal of Agricultural Water Management. 224, 106544.
Amoaghaee, R., Mostajeran, A. Emtiazee, G., 2003. The effect of Azospirillum on some growth and yield indices of three wheat cultivars. Journal of Agricultural Science and Technology and Natural Resources. 7, 127-139. [In Persian with English summary].
Atrashi, M., Vafadar Estehan, F., Amoaghaee, R., 2015. The effect of mycorrhizal fungi and rhizobacteria that stimulate plant growth on growth rate, flowering time and stevioside accumulation pattern in stevia. Iranian Journal of Medicinal and Aromatic Plants Research. 31, 220-234. [In Persian with English summary].
Bashan, Y., Holguin, G., 1997. Azospirillum-plant relationships: environmental and physiological advances (1990-1996). Canadian Journal of Microbiology. 43, 103-121.
Bayat, S., Sepehri, A., Zareaabyane, H., Abdolahi, M.R., 2010. Effects of salicylic acid and paclobutrazol on maize yield under salt stress. 11th Congress of Agronomy and Plant Breeding, Shahid Beheshti University, Tehran. 3715-3718. [In Persian].
Black, C., Erick, B., Ong, C., 2000. Utilization of light and water in tropical agriculture. Agricultural and Forest Meteorology. 104, 25-47.
Çakmakçi, R., Erat, M., Oral, B., Erdogan, U., Şahin, F., 2009. Enzyme activities and growth promotion of spinach by indole-3-acetic acid-producing rhizobacteria. Journal of Horticultural Science and Biotechnology. 84, 375–380.
Davis, T. D., Steffens, G. L., Sankhala, N., 1988. Triazole plant growth regulators. pp. 63 – 96, In: J. Janick (Ed.), Horticultural Reviews. Timber Press, USA.
Dewi, K., Agustina, R. Z., Nurmalika, F., 2016. Effects of blue light and paclobutrazol on seed germination, vegetative growth and yield of black rice (Oryza sativa L. ‘CempoIreng’). Biotropia, 23, 85–96. https://doi.org/10.11598/btb.2016.23.2.478
Dobbelaere, S., Croonenborghs, A., Thys, A., Ptacek, D., Vanderleyden, J., Dutto, P., 2001. Response of agronomically important crops to inoculation with Azospirillum. Australian Journal of Plant Physiology. 28, 871-879.
Fletcher, R., Hofstra, G., 1988. Triazoles as potential plant protectants. In: Berg, D., Plempel, M. (eds.), Sterol synthesis inhibitors in plant protection, Ellis Horwood Ltd., Cambridge, pp. 321-331.
Fletcher, R., Sankhla, N., Davis, T., 2000. Triazoles as plant growth regulators and stress protectants. Horticultural Reviews. 24, 55-122.
Grover, M., Ali, S. Z., Sandhya, V., Rasul, A., Venkateswarlu, B., 2010. Role of microorganisms in adaptation of agriculture crops to abiotic stress. World Journal of Microbiology and Biotechnology. 27, 1231-1240.
Hamidi, A., Asgharzadeh, A., Chaokan, R., Dehghan shoar, M., Qalavand, A., Malakouti, J., 2010. Effects of PGPR application on dry matter partitioning and some growth characteristics of maize (Zea mays L.) hybrids under greenhouse conditions. Iranian Journal of Soil Research. 24, 55-67. [In Persian with English summary]
Jalali, M., Zare, A.A., 2019. Effect of plant growth promoting rhizobacteria on quantitative and qualitative yield of Lemon verbena. 16th Iranian Soil Science Congress. University of Zanjan, August 27-29. [In Persian].
Jungklang, J., Saengnil, K.,, Uthaibutra, J., 2016. Effects of water-deficit stressand paclobutrazol on growth, relative water content, electrolyte leakage, prolinecontent and some antioxidant changes in Curcuma alismatifolia Gagnep. cv. Chiang Mai Pink. Saudi Journal of Biological Sciences, 24, 1505–1512.
Kamran, M., Wennan, S., Ahmad, I., Xiangping, M., Wenwen, C., Xudong, Z., Tiening, L., 2018. Application of paclobutrazol affect maize grain yield by regulating root morphological and physiological characteristics under a semi-arid region. Scientific. Reports. https://doi.org/10.1038/s41598-018-23166-z
Kasim, W.A., Osman, M.E., Omar, M.N., Abd El-Daim, I.A., Abd El-Daim, I.A., Bejai, S., Meijer, J., 2013. Control of drought stress in wheat using plant-growth- promoting bacteria. Journal of Plant Growth Regulation. 32, 122–130.
Khan, M.S.H., Wagatsuma, T., Akhter, A., Taukraya, K., 2009. Sterol biosynthesis inhibition by paclobutrazol induced greater Aluminum sensivity in Al-tolerant rice. American Journal of Plant Physiology. 4, 89-99.
Kim, Y. C., Glick, B., Bashan, Y., Ryu, C.M., 2013. Enhancement of plant drought tolerance by microbes. In: Aroca R, (eds). Plant responses to drought stress. Berlin: Springer Verlag, 383-413.
Kong L., Wang, F., Si, J., Feng, B., Zhang, B., Li, S., Wang, Z., 2014. Increasing in ROS levels and callose deposition in peduncle vascular bundles of wheat (Triticum aestivum L.) grown under nitrogen deficiency. Journal of Plant Interactions. 8, 109–116.
Kuan-Hung, L., Fu-Hsiang, P., Shih-Ying, H., Hsiao-Feng, L., 2006. Pre-treating paclobutrazol enhanced chilling tolerance of sweet potato. Journal of Plant Growth Regulation. 49, 249-262.
Kumar, R., Bhatia, R., Kukreja, K., Behl, R.K., Dudeja, S.S., Narula, N., 2007. Establishment of Azotobacter on plant roots: chemotactic response, development and analysis of root exudates of cotton (Gossypium hirsutum L.) and wheat (Triticum aestivum L.). Journal of Basic Microbiology. 47, 436-439. https://doi.org/10.1002/jobm.200610285
Liu, X-M., Zhang, H., 2015. The effects of bacterial volatile emissions on plant abiotic stress tolerance. Frontiers in Plant Science. 6, 1-6, 774.
Mahmut Sinan, T., Rahmi, D., Guleray, A., 2009. Determination of effects of some plant growth regulators (PGRs) on changes of some isoenzymes in bean (Phaseolus vulgaris L. cv Terzibaba) at chilling temperatures: in gel enzyme assays. Romanian Biotechnological Letters. 14, 4858-4869.
Marasco, R., Rolli, E., Ettoumi, B., Vigani, G., Mapelli, F., Borin, S., Abou-Hadid, A.F., El-Behairy, U.A., Sorlini, C., Cherif, A., 2012. A drought resistance promoting microbiome is selected by root system under desert farming. Plos One. 7, 1-14.
Mostajeran, A., Amoaghaee, R., Emtiazee, G., 2006. Effect of azospirillum and salinity of irrigation water on grain yield and protein content of wheat cultivars. Research Journal of University of Isfahan "Science". 24, 51-64. [In Persian with English Summary].
Mozaffari, A., Habibi, D., Asgharzadeh, A., Mashhadi Akbarbojar, M., 2016. Drought tolerance study of two wheat cultivars inoculated with Rhizobacteria (PGPR). Journal of Agriculture and Plant Breeding. 7-4, 29-43. [In Persian with English summary].
Munns, R., James, R.A., Lauchli, A., 2006. Approaches increasing the salt tolerance of wheat and other cereals. Journal of Experimental Botany. 57, 1025-1043.
Narula, N., Kumar, V., Singh, B., Bhatia, R., Lakshminarayana, K., 2005. Impact of biofertilizers on grain yield in spring wheat under varying fertility conditions and wheat-cotton rotation. Archives of Agronomy and Soil Science. 51, 79-89.
Paknejad, F., Majidi, A., Nourmohammadi, Gh., Sayyidat, A., Vazan, S., 2007. Evaluation of the effect of drought stress on the traits affecting the accumulation of grain material in different wheat cultivars. Journal of Agricultural Sciences. 13, 137-148. [In Persian with English summary].
Rady, M.M., Gaballah, M.S., 2012. Improving barley yield grown under water stress conditions. Research Journal of Recent Sciences. 1, 1–6.
Robin, A., Vansuyt, G., Hinsinger, P., Meyer, J.M., Briat, J.F., Lemanceau, P., 2008. Iron dynamics in the rhizosphere: consequences for plant health and nutrition. Advances in Agronomy. 99, 183-225.
Sadeghi-Shoae, M., Habibi, D., Taleghani, D., Paknejad, F., Kashani, A., 2014a. Evaluation the effect of paclobutrazol on bolting, qualitative and quantitative performance in autumn sown-sugar beet genotypes in Moghan region. International Journal of Biosciences. 5, 345-354.
Sadeghi-Shoae, M., Paknejad, F., Shahbazpanahi, B., Tookalloo M.R., 2014b. Florescence parameters, chlorophyll content and relative water content (RWC) of wheat varieties as affected by different regimes of irrigation. International Journal of Biosciences. 4, 135-140.
Sankar, B., Karthishwaran, K., Somasundaram, R., 2013. Leaf anatomychanges in peanut plants in relation to drought stress with or withoutpaclobutrazol and abscisic acid. Journal of Phytology. 5, 25–29.
Si, Zh., Zain, M., Mehmood, F., Wang, G., Gao, Y., Duan, A., 2020. Effect of nitrogen application rate and irrigation regime on growth, yield, and water-nitrogen use efficiency of drip-irrigated winter wheat in the North China Plain. Journal of Agricultural Water Management. 231, 1-8.
Swedrzynska, D., Sawicka, A., 2000. Effect of inoculation with Azospirillum brasilense on development and yielding of maize under different cultivation conditions. Polish Journal of Enviromental Studies. 9, 505-509.
Syahputra, B.S.A., Sinniah, U.R., Ismail, M.R., Swamy, M.K., 2016. Optimization of paclobutrazol concentration and application time for increased lodging resistance and yield in field-grown rice. Philippine Agricultural Sciences. 99, 221–228
Tafazoli, E., Beyl, C., 1993. Changes in endogenous abscisic acid and cold hardiness in actinidia treated with triazole growth retardants. Journal of Plant Growth Regulation. 12, 79-83.
Van Loon, L.C., 2007. Plant responses to plant growth promoting rhizobacteria. European Journal of Plant Pathology. 119, 243-254.
Verma, P., Saxena, R., Tomar, R.S., 2016. Rhizobacteria: a promising tool for drought tolerance in crop plants. International Journal of Pharma and Bio Sciences Spl Ed. (Int-BIONANO-2016). 116-125.
Vessey, J.K., 2003. Plant growth promoting rhizobacteria as biofertilizers. Plant and Soil. 255, 571-586.
Werbrouck, S.P.O., Debergh, P.C., 1996. Imidazole fungicides and paclobutrazol enhance cytokinin-induced adventitious shoot proliferation in Araceae. Journal of Plant Growth Regulation. 15, 81-85.
Wilhelm, R.A., Hansjoerg, F.R., Jan, E.G., Hubert, S.A., Johannes, J.U., 1987. Tetcyclacis and triazole-type plant growth retardants: their influence on the biosynthesis of gibberellins and other metabolic processes. Post Mangement Science. 21, 241-252.
Yan, W., Yanhong, Y., Wenyu, Y., Taiwen, Y., Weiguo, L., Xiaochun, W., 2013. Responses of root growth and nitrogen transfer metabolism to uniconazole, a growth retardant, during the seedling stage of soybean under relay strip intercropping system. Communications in Soil Science and Plant Analysis. 44, 3267–3280. https://doi.org/10.1080/00103624.2013.840838
Yang, J., Kloepper, J.W., Ryu, C.M., 2009. Rhizosphere bacteria help plants tolerate abiotic stress. Trends in Plant Science. 14, 1–4.
Zaied, K. A., Abd, A.H., El-Hady, A., Sharief. A. H., Ashour, E.H., Nassef, M.A., 2007. Effect of Horizontal DNA transfer in Azospirillum and Azotobacter strains on biological and biochemical traits of non legume plants. Journal of Applied Sciences Research. 3, 73-86.
Zhao, J., Zhang, W., Qiu, Q., Li, Z.P., Zhang, M.H., Yan, X.Y., Du, D.H., 2012. Effects of PP333 spraying at different stages on soybean agronomic and physiological characters. Soybean Sciences. 30, 211–214