نوع مقاله : مقاله پژوهشی
نویسندگان
گروه زیست‌شناسی گیاهی و جانوری، دانشکده علوم و فناوری‌های زیستی، دانشگاه اصفهان، اصفهان
چکیده
در شرایط تنش، گونه های فعال اکسیژن (ROS) در برگ ها تجمع پیدا می کند و به اکسیداسیون ترکیبات مهم سلولی مثل پروتئین، کلروفیل، لیپید و اسیدهای نوکلئیک منجر می شود. در این پژوهش گیاهان باززایی شده از ریشه تنباکو و گیاهان غیرباززایی شده به مدت 4هفته در محیطMS حاوی غلظت های صفر، 100، 200 میلی مولارNaCl رشد داده شدند و سپس شاخص های رشد از جمله وزن تر و خشک، میزان رنگیزه های فتوسنتزی شامل کلروفیل کل و کاروتنوئید، غلظت های سدیم و پتاسیم، میزان فنل کل، میزان پرولین، میزان آنتی اکسیدانی کل، میزان ROS کل، میزان پراکسیداسیون لیپیدها، میزان اکسین و همچنین آنالیز RAPID-PCR مورد بررسی قرارگرفت. مشاهده شد وزن تر و خشک گیاهان باززایی شده افزایش معنی داری را نسبت به گیاهان غیر باززایی نشان داد. همچنین افزایش میزان رنگیزه ها و نیز کاهش سدیم و افزایش پتاسیم و کاهش ROS کل و MDA و افزایش آنتی اکسیدانی کل و اکسین نیز نسبت به گیاهان غیرباززایی در شرایط شوری مشاهده شد. نتایج به دست آمده از آنالیز RAPID-PCR وجود تنوع سوماتیکی را در گیاهان باززایی شده نسبت به گیاهان غیرباززایی نشان داد. تحقیق حاضر نشان می دهد گیاهان باززایی شده می توانند باعث افزایش مقاومت به شوری و بهبود شاخص های رشد در شرایط شوری شوند.
کلیدواژه‌ها
موضوعات
Afshari Pour, S., 1993. Basics of plant tissue culture. Vice Chancellor for Research, Isfahan University of Medical Sciences, 247 pages. [In Persian].
Aeturi, M.J. Aulicino, M.B. Collado, M.B., Molina, M.C., 2009. Evalunation salinity tolerance at seedling stage in maize (Zea mays L.). Maize Genetics Cooperation Newsletter. 83, 1-4.
Azooz, M.M., Shaddad, M.A., Abdel-Latef, A.A., 2004. Leaf growth and K+/Na+ ratio as an indication of the salt tolerance of three sorghum cultivars grown under salinity stress and IAA treatment. Acta Agronomica Hungarica. 52, 287-296.
Aloni, R., 2001. Foliar and axial aspects of vascular differentiation: hypotheases and evidence. Journal of Plant Growth Regulation. 20, 22-34.
Alscher, R.G., Erturk, N. Health, L.S., 2002. Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. Journal of Experimental Botany. 53, 1331-1341.
Alcazar, R., Marco, F., Cuevas, J. C., Patron, M., Ferrando, A., Carrasco, P. Altabella, T., 2006. Involvement of polyamines in plant response to abiotic stress. Biotechnology Letters. 28, 1867-1876.
Bates, L.S, Waldren, R.P, Teare, I.D., 1973. Rapid determination of free proline for water-stress studies. Plant and Soil. 39, 205-207.
Bybordi, A., 2010. The influence of salt stress on seed germination, growth and yied of canola cultivars. Notulae Botanicae Horti Agrobotanici Chj-Napoca. 38, 128-133.
Chaves, M.M., Oliveira, M.M., 2004. Mechanisms underlying plant resilience to water deficits: prospects for water-saving agriculture. Journal of Experimental Botany. 55, 2365-2384.
Dogan, M., 2011. Antioxidative and proline potentials as a protective mechanism in soybean plants under salimity stress. African Journal of Biotechnology. 10, 5972-5978.
Ehsanpour, A.A., Amini, F., 2002. Plant Tissue Culture. Edition 2, Isfahan University Jihad Publications. 181 pages. [In Persian].
Eklof, S., Astot, C., Sitbon, F., Moritz, T., Olsson, O., Sandberg, G., 2000. Transgenic tobacco plants co-expressing Agrobacterium iaa and ipt genes have wild-type hormone levels but display both auxin-and cytokinin-overproducing phenotypes. The Plant Journal. 23, 279-284.
Farooq, M., Wahid, A., Kobayashi, N, Fujita, D.B.S.M.A., Basra, S.M.A. 2009. Plant drought stress: effects, mechanisms and management. Sustainable Agriculture. 153-188.
Hansen, H., Grossmann, K. 2000. Auxin-induced ethylene triggers abscisic acid biosynthesis and growth inhibition. Plant physiology. 124, 1437-1448.
Lichtenthaler‚ H.K. 1987. Chlorophylls and carotenoids: pigments of photosynthetic biomembrances. Methods in Enzymology. 148‚ 350-382.
Ljung, K., Bhalerao, R.P., Sandberg, G., 2001. Sites and homeostatic control of auxin biosynthesis in Arabidopsis during vegetative growth. The Plant Journal. 28, 465-474.
Ludlow, M.M., Muchow, R.C., 1990. A critical evaluation of traits for improving crop yieds in water-limited environments. Advances in Agronomy. 43, 107-153.
Mahalingam, R., Jambunathan, N., Gunjan, S. K., Faustin, E., Weng, H.U.A., Ayoubi, P., 2006. Analysis of oxidative signalling induced by ozone in Arabidopsis thaliana. Plant, Cell and Environment. 29, 1357-1371.
Miguel, C., Marum, L., 2011. An epigenetic view of plant cells cultured in vitro: somaclonal variation and beyond. Journal of Experinental Botany. 62, 3713-3725.
Muhlenbock, P., Szechynska-Hebda, M., Plaszczyca, M., Baudo, M., Mateo, A., Mullineaux, P.M., Karpinski, S., 2008. Chloroplast signaling and LESION SIMULATING DISEASEI regulate crosstalk between light acclimation and immunity in Arabiddopsis. The Plant Cell. 20, 2339-2356.
Palni, L.M.S., Burch, L., Horgan, R., 1988. The effect of auxin concentration on cytokinin stability and metabolism. Planta. 174, 231-234.
Pencik, A., Simonovik, B., Petersson, S.V., Henykova, E., Simon, S,. Greenham, K., Ljung, K., 2013. Regulation of auxin homeostasis and gradients in Arabidopsis roots through the formation of the indole-3-acetic acid catabolite 2-oxindole-3-acetic acid. The Plant Cell, 25, 3858-3870.
Rani, V., Raina, S.N., 2000. Genetic fidelity of organized meristem-derived micropropagated plants: critical reappraisal. In Vitro Cellular and Developmental Biology-Plant. 36, 319-330.
Sharp, R.E., LeNoble, M.E., 2002. ABA, ethylene and the control of shoot and root growth under water stress. Journal of Experimental Botany. 53, 33-37.
Singh, R.J., Kollipara, K.P., Hymowitz, T. 1987. Intersubgeneric hybridization of soybeans with a wild perennial species, Glycine clandestina Wendl. Theoretical and Applied Genetics. 74, 391-396.
Santos, C.V., 2004. Regulation of chlorophyll biosynthesis and degradation by salt stress in sunflower leaves. Scientia Horticulturae. 103, 93-99.
Saradhi, P.P., AliaArora, S., Prasad, K.V.S.K., 1995. Prolin accumulates in plants exposed to UV radiation and protects them against UV-induced peroxidation. Biochemical and Biophysical Research Communication. 209, 1-5.
Santoro, M.M., Liu, Y., Khan, S.M., Hou, L.X., Bolen, D.W., 1992. Increased thermal stability of proteins in the presence of naturally occurring osmolytes. Biochemistry. 31, 5278-5283.
Scandalios, J.G., 1993. Oxygen stress and superoxide dismutases. Plant physiology. 101, 7-12.
Sponchiado, B.N., White, J.W., Castillo, J.A., Jones, P.G., 1989. Root growth of four common bean cultivars in relation to drought tolerance in environments with contrasting soil types. Experimental Agriculture. 25, 249-257.
Strader, L.C., Monroe-Augustus, M., Bartel, B., 2008. The IBR5 phosphatase promotes Arabidopsis auxin responses through a novel mechanism distinct from TIR1-mediated repressor degradation. BMC Plant Biology. 8, 1-15.
Tognetti, V.B., Muhlenbock, P.E.R., Van Breusegem, F., 2012. Stress homeostasis-the redox and auxin perspective. Plant, Cell & Environment. 35, 321-333.
Woodward, A.W., Bartel, B., 2005. Auxin: regulation, action, and intraction. Annals of Botany. 95, 707-735.
Zahir, Z.A., Arshad, M., Azam, M., Hussain, A., 1997. Effect Of an auxin precursor tryptophan and Azotobacter inoculation on yied And chemical composition of potato under fertilized conditions. Journal of Plant Nutrition. 20, 745-752.
Zolman, B.K., Bartel, B., 2004. An Arabidopsis indole-3-butyric acid-response mutant defective in PEROXIN6, an apparent ATPase implicated in peroxisomal function. Proceedinges of the National Academy of Sciences. 101, 1786-1791.
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