نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، دانشکده‌ی کشاورزی، دانشگاه فردوسی مشهد، مشهد

2 استادیار، گروه باغبانی، دانشکده‌ی کشاورزی، دانشگاه فردوسی مشهد، مشهد

چکیده

به منظور ارزیابی اثر کاربرد قارچ تریکودرما در شرایط سطوح مختلف آبیاری بر خصوصیات رشدی و جذب برخی عناصر در گیاه ریحان آزمایشی به صورت فاکتوریل در قالب طرح کاملا تصادفی با سه تکرار در سال 1397 در گلخانه اجرا شد. تیمارهای مورد بررسی شامل 3 سطح آبیاری (50، 75 و 100 درصد ظرفیت زراعی) به عنوان فاکتور اول و مایه‌زنی با قارچ تریکودرما (غلظت 108 ) و عدم مایه‌زنی با قارچ تریکودرما به عنوان فاکتور دوم بودند. نتایج نشان داد کاربرد قارچ T. harzianum در سطوح مختلف آبیاری اثر معنی‌داری بر صفات رشدی و جذب عناصر در گیاه ریحان داشت. بیشترین میزان وزن تر (39.27 گرم در بوته) و خشک اندام هوایی (5.916 گرم در بوته) و وزن تر (5.365 گرم در بوته) و خشک ریشه (2.129 گرم در بوته) و سطح برگ (11.828 سانتیمتر مربع) در تیمار سطح آبیاری 100 درصد ظرفیت زراعی همراه با کاربرد قارچ تریکودرما مشاهده شد. همچنین بیشترین میزان جذب عنصرهای فسفر(0.0038 میلی‌گرم بر گرم وزن خشک)، پتاسیم (0199/0 میلی‌گرم بر گرم وزن خشک) و نیتروژن (0.01637 میلی‌گرم بر گرم وزن خشک) در تیمار سطح آبیاری 100 درصد ظرفیت زراعی همراه با کاربرد قارچ تریکودرما مشاهده شد. بر اساس نتایج حاصل از این پژوهش به طور کلی می‌توان بیان کرد کاربرد قارچ T. harzianum همراه با گیاه ریحان در شرایط سطوح مختلف آبیاری قابلیت بهبود رشد گیاه را داشته و منجر به افزایش کارایی گیاه و جذب مواد غذایی می‌شود.

کلیدواژه‌ها

موضوعات

 
Altomare, C., Norvell, W. A., Björkman, T., Harman, G.E., 1999. Solubilization of phosphates and micronutrients by the plant-growth-promoting and biocontrol fungus Trichoderma harzianum Rifai 1295-22. Applied and Environmental Microbiology. 65, 2926-2933.
Alwhibi, M.S., Hashem, A., Abd-Allah, E.F., Alqarawi, A.A, Soliman, D.W.K., Wirth, S., Egamberdieva, D., 2017. Increased resistance of drought by Trichoderma harzianum fungal treatment correlates with increased secondary metabolites and proline content. Journal of Integrative Agriculture. 16, 1751–1757.
Amiri Deh Ahmadi, S.R., Rezvani Moghaddam, P., Ehyaee, H.R., 2012. The effects of drought stress on morphological traits and yield of three medicinal plants (Coriandrum sativum, Foeniculum vulgare and Anethum graveolens) in greenhouse conditions. Iranian Journal of Field Crops Research. 10, 116-124. [In Persian with English Summary].
Araghi, M.M., Rahnama, K., Latifi, N., 2012. Evaluation of growth increasing effect of Trichoderma harzianum on tomato. Journal of Plant Production Research. 18, 107-118. [In Persian with English Summary].
Ashraf, M., Shahbaz, M., Ali, Q., 2013. Drought-induced modulation in growth and mineral nutrients in canola (Brassica napus L.). Pakistan Journal of Botany. 45, 93-98.
Aslani, Z., Hassani, A., Rasooli Sadaghiyani, M., Sefidkon, F., Barin, M., 2011. Effect of two fungi species of arbuscular mycorrhizal (Glomus mosseae and Glomus intraradices) on growth, chlorophyll contents and P concentration in Basil (Ocimum basilicum L.) under drought stress conditions. Iranian Journal of Medicinal and Aromatic Plants Research. 27, 471-486. [In Persian with English Summary].
Azarmi, R., Hajieghrari, B., Giglou, A., 2011. Effect of Trichoderma isolates on tomato seedling growth response and nutrient uptake. African Journal of Biotechnology. 10, 5850– 5855.
Benitez, T., Rincon, A.M., Limon, M.C., Codon, A.C., 2004. Biocontrol mechanisms of Trichoderma ssp. strains. International Microbiology. 7, 249-260.
Chacon, M.R., Rodríguez-Galán, O., Benítez, T., Sousa, S., Rey, M., Llobell, A., Delgado-Jarana1, J., 2007. Microscopic and transcriptome analyses of early colonization of tomato roots by Trichoderma harzianum. International Microbiology. 10, 19-27.
Chang, C., Chang, Y., Baker, R., Kleifield, O., Chet, I., 1986. Increased growth of plants in the presence of the biological control agent Trichoderma harzianum. Plant Disease. 70, 145-148.
Chapman H.D, Pratt P.F., 1982. Method of Analysis for Soils, Plants and Waters. University of California. Agriculture and Natural Resources Publication, Los Angeles.
Cuevas, C., 2006. Soil inoculation with Trichoderma pseudokoningii rifai enhances yield of rice. Philippine Journal of Science. 135, 31-37.
Emami, A., 1996. Methods of plant analysis. Soil and Water Research Institute, Technical Publication. 982, 11-28. [In Persian].
Farias, L.N., Bonfim-Silva, E.M., Pietro-Souza, W., Vilarinho, M.K.C., Silva, T.J.A., Guimarães, S.L., 2013. Características morfológicas e produtivas de feijão guandu anão cultivado em solo compactado. Revista Brasileira de Engenharia Agrícola e Ambiental. 17, 497-503.
Hamada, A.M, and EL-enany, A.E., 1994. Effect of NaCl salinity on growth, pigment and mineral element contents, and gas exchange of broad bean and pea plants. Biologia Plantarum. 36, 75- 81.
Harman, G.E., Howell, C.R., Viterbo, A., Chet, I., Lorito, M., 2004. Trichoderma species-opportunistic, avirulent plant symbionts. Nature Reviews. 2, 43-56.
Harman, G.E., Petzoldt, R., Comis, A., Chen, J., 2003. Interactions between Trichoderma harzianum strain T22 and maize inbred line Mo17 and effects of these interactions on diseases caused by Pythium ultimum and colletotrichum graminicola. The American Phytopathological Society. 94, 147 -153.
Hashem, A., Abd-Allah, E.F., Alqarawi, A.A., Al-Huqail, A.A., Egamberdieva, D., 2014. Alleviation of abiotic salt stress in Ochradenus baccatus by Trichoderma hamatum. Journal of Plant Interactions. 9, 857-868.
Hoyos-Carvajal, L., Orduz, S., Bissett, J., 2009. Growth stimulation in bean (Phaseolus vulgaris L.) by Trichoderma. Biological Control. 51, 409-416.
Irannezhad, A., Vatanpour Azghandi, A., Rahnama, H., Jaliani, N., Bozorgipour, R., 2010. Improvement of rooting and aclimatization of tissue cultured plantlets of olive (Olea europaea L. cv. Zard) by Agrobacterium rhizogenes. Seed and Plant Production Journal, 26, 85-93. [In Persian with English Summary].
Jabbarzadeh, J., Kaviani, M.H., Ghasemi, N., Mohandessi, A.R. Safarian, S., 2010. Effect of Trichoderma harzianum T22 (TRIANUM-P®) on decreasing infection of soil-born diseases and improvement of tomato (Lycopersicon esculentum) quality factors in greenhouses of Tehran region. P. 823. In: K. Benanaj (ed.), Proceedings of the 19th Iranian Plant Protection Con-gress, 31 Jul. 2010. Iranian Plant Protection Research Institute, Tehran, Iran. [In Persian].
Jalali, Z., Shoor, M., Rouhani, H., 2014. Investigation of the effect of Trichoderma and different levels of iron on morphological and biochemical traits of willow leaves. P. 1-4. In: P. Azadi (ed), The First National Congress of Ornamental Flowers and Plants, 21-23 Oct. 2014. National Research Institute of Flowers and Ornamental Plants. Karaj, Iran. [In Persian].
Kleifield, O, Chet, I., 1992. Trichoderma – plant interaction and its effect on increased growth response. Plant and Soil. 144, 267–272.
Kramer, P.J, Boyer, J.S., 1995. Water Relations of Pants and Soils. Academic Press, New York.
Kumar, A., Mangla, C., Aggarwal, A., Srivastava, V., 2014. Rhizospheric effect of Endophytic mycorrhiza and Trichoderma Viride on physiological parameters of Mentha Spicata linn. Asian Journal of Advanced Basic Sciences. 2, 99-104.
Li, Y.T., Hwang, S.G., Huang, Y.M., Huang, C.H., 2018. Effects of Trichoderma asperellum on nutrient uptake and Fusarium wilt of tomato. Crop Protection. 110, 275–282.
Lopez-Mondejar, R., Bernal-Vicente, A., Ros, M., Tittarelli, F., Canali, S., Intrigiolo, F., Pascual, J.A., 2010. Utilisation of citrus compost-based growing media amended with Trichoderma harzianum T-78 in Cucumis melo L. seedling production. Bioresource Technology. 101, 3718-3723.
Mastouri, F., Bjorkman, T., Harman, G.E., 2010. Seed treatment with Trichoderma harzianum alleviates biotic, abiotic, and physiological stresses in germinating seeds and seedlings. Phytopathology. 100, 1213–1221.
Masunaka, A., Hyakumachi, M. Takenaka, S., 2011. Isoflavonoid phytoalexin vestitol production for colonization on/in the roots of Lotus japonicas. Microbes and Environment. 26, 128-134.
Mauad, M., Crusciol, C.A.C., Grassi Filho, H., 2011. Produçãode massa seca e nutrição de cultivares de arroz de terras altas sob condições de déficit hídrico e adubação silicatada. Semina. Ciências Agrárias, 32, 939-948.
Mazhabi, M., Nemati, H., Rouhani, H., Tehranifar, A., Moghadam, E.M., Kaveh, H., Rezaee, A., 2011. The effect of Trichoderma on polianthes qualitative and quantitative properties. Journal of Animal and Plant Sciences. 21, 617-621.
Mehrabi-Koushki, M., Rouhani, H., Mahdikhani-Moghaddam, E., 2012. Differential display of abundantly expressed genes of Trichoderma harzianum during colonization of tomato-germinating seeds and roots. Current Microbiology. 65, 524–533.
Moghaddam, M., Omidbeygi, R., Salimi, A., Naghavi, M.R., 2013. An assessment of genetic diversity among Iranian populations of basil (Ocimum spp.) using morphological traits'. Iranian Journal of Horticultural Science. 44, 227-243. [In Persian with English Summary].
Mohammadi-kashka, F., Pirdashti, H., Yaghoubian, Y., Baharisaravi, H., 2015. Effect of coexistence of Trichoderma, quasi-mycorrhizal fungi and phosphate-soluble bacteria on the photosynthetic pigments of the pepper plant (Capsicum annuum L). 4th National Congress of Organic and Conventional Agriculture. 19-20 Aug. 2015. Ardabil. Iran. [In Persian with English Summary].
Omidbeygi, R., 2006. Approaches to the Production of Medicinal Plants. Astan Quds Razavi Publisher. [In Persian].
Osiewacz H.D., 2002. Molecular Biology of Fungal Development. Marcel Dekker. New York.
Ozbay, N., Newman, S.E., Brown, W.M., 2004. The effect of the Trichoderma harzianum strains on the growth of tomato seedlings. Acta Horticulturae. 635, 131- 135.
Paton, A., 1992. Asynopsis of Ocimum L. (Labiatae) in Africa. Kew Bulletin, 47, 403-435.
Pushpangadan, P, Bradu, B.L., 1995. Advances in Horticulture, Medicinal and Aromatic Plants, Volume 11. Malhotra Publishing House, New Delhi
Salardini, A.A., 1993. Soil Fertility. Tehran University Press, Tehran, Iran. [In Persian].
Salari, E., Rouhani, H., Mahdikhani-Moghaddam, E., Saberi Riseh, R., Mehrabi-Koushki, M., 2014. Efficacy of two methods ’’seed coating’’ and ’’soil application’’ of Trichoderma on growth parameters of tomato plant. Journal of Plant Protection. 28, 500-507. [In Persian with English Summary].
Shukla, N., Awasthi, R.P., Rawat, L., Kumar, J., 2012. Biochemical and physiological responses of rice (Oryza sativa L.) as influenced by Trichoderma harzianum under drought stress. Plant Physiology and Biochemistry. 54, 78-88.
Silva, T.R., Cazetta, J.O., Carlin, S.D., Telles, B.R., 2017. Drought-induced alterations in the uptake of nitrogen, phosphorus and potassium, and the relation with drought tolerance in sugar cane. Ciência e Agrotecnologia. 41, 117-127.
Singh, S.P., Singh, H.B., Singh, D.K., 2013. Effect of Trichoderma harzianum on mineral component and antioxidant activity of tomato fruits. Vegetos. 26, 237-244.
Singh, V., Singh, P.N., Yadav, R.L., Awasthi, S.K., Joshi, B.B., Singh, R.K., Lal, R.J., Duttamajumder, S.K., 2010. Increasing the efficacy of Trichoderma harzianum for nutrient uptake and control of red rot in sugarcane. Journal of Horticulture and Forestry. 2, 66-71.
Taiz, L, Zeiger, E., 2006. Plant physiology. 4th ED. Sinauer Associates, Inc, Publishers Sunderland, Massachusetts.
Tallapragada, P., 2013. Study on growth and biomass of Ocimum sanctum plants and protein profiling of Trichoderma harzianum. International Journal of Pharma and Bio Sciences. 0975-6299.
Vinale, F., Sivasithamparam, K., Ghisalberti, E.L., Marra, R., Wooa, S.L., Lorito, M., 2008. Trichoderma-plant-pathogen interactions. Soil Biology and Biochemistry. 40, 1-10.
Yedidia, I., Srivastava, A.K., Kapulnik, Y., Chet, I., 2001. Effect of Trichoderma harzianum on microelement concentrations and increased growth of cucumber plants. Plant and Soil. 235, 235-242.