نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد بیوتکنولوژی کشاورزی، گروه تولید و ژنتیک گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه ارومیه، ارومیه

2 استاد، گروه تولید و ژنتیک گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه ارومیه، ارومیه

3 دانشیار، گروه تولید و ژنتیک گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه ارومیه، ارومیه

4 دانشیار، گروه مهندسی آب، دانشکده کشاورزی و منابع طبیعی، دانشگاه ارومیه، ارومیه

چکیده

به‌منظور بررسی تأثیر تنش خشکی بر بیان عوامل رونویسی AP2-Domain، HD-ZIP، WRKY و MYB در آفتابگردان روغنی، دو لاین با واکنش متفاوت انتخاب و در قالب طرح کاملاً تصادفی با 3 تکرار در گلخانه در سال زراعی 1395-1396 کشت شدند. گیاهان تا مرحله 8 برگی از لحاظ وضعیت آبی در 100 درصد ظرفیت زراعی نگهداری شدند. بعد از این مرحله تعدادی از گلدان‌ها در همان ظرفیت زراعی نگهداری شدند، اما به بقیه تنش خشکی 80، 60 و 40 درصد ظرفیت زراعی اعمال شد. نمونه برداری از گیاهان یک و سه هفته بعد از اعمال تنش انجام گرفت. مطالعه بیان ژن‌های مذکور با استفاده از تکنیک واکنش زنجیره ای پلی مراز در زمان واقعی انجام شد. مقایسات میانگین بیان عوامل رونویسی AP2-Domain، WRKY و MYB در دو لاین ENSAT254 (متحمل) و LC1064C(حساس) نشان داد که بیان ژن‌ها یک هفته بعد از اعمال تنش در شدتْ تنش‌های مختلف محسوس نبوده اما در هفته سوم بعد از اعمال تنش در شدت تنش 40 درصد میزان بیان ژن‌ها خصوصا در لاین ENSAT254 افزایش یافته است. در رابطه با عامل رونویسی HD-ZIP میزان بیان یک هفته بعد از اعمال تنش در شدت تنش 40 درصد در لاین ENSAT254خیلی بیشتر از لاین LC1064C بود. میزان بیان در هفته سوم بعد از اعمال تنش در هر دو لاین در شدت تنش 40 درصد افزایش یافت، هر چند افزایش بیان در لاین LC1064C اندکی بیشتر بود. بنابراین، به نظر می‌رسد بیان زود هنگام عامل رونویسی HD-ZIP در افزایش مقاومت ژنوتیپ به تنش خشکی دخیل باشد. یافته های این تحقیق می‌تواند در برنامه‌های اصلاحی آفتابگردان برای تولید و توسعه ارقام متحمل به تنش خشکی مفید و فایده واقع شود.

کلیدواژه‌ها

Abe, H., Urao, T., Ito, T., Seki, M., Shinozaki, K., Yamaguchi- Shinozaki, K., 2003. Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. Plant Cell. 15, 63-78.
Agarwal, M., Hao, Y., Kapoor, A., Dong, C. H., Fuji, H., Zheng, X., Zhu, J. K., 2006. A R2R3 type MYB transcription factor is involved in the cold regulation of CBF genes and in acquired freezing tolerance. Journal of Biological Chemistry. 281, 37636-37645.
Aharoni, A., Dixit, S., Jetter, R., Thoenes, E., Van Arkel, G., Pereira, A., 2004. The SHINE clade of AP2 domain transcription factors activates wax biosynthesis, alters cuticle properties, and confers drought tolerance when overexpressed in Arabidopsis. The Plant Cell. 16, 2463-2480.
Athar, H.R. Ashraf, M., 2005. Photosynthesis under drought stress. In: Pessarakli, M. (ed.), Handbook of Photosynthesis. CRC Press, Taylor and Francis Group, New York, pp. 793-804.
Brunner, E., Domhof, S., Langer, F., 2002. Nonparametric Analysis of Longitudinal Data in Factorial Experiment. New York: Wiley.
Casaretto, J. Ho, T.H., 2003. The transcription factors HvABI5 and HvVP1 are required for the abscisic acid induction of gene expression in barley aleurone cells. Plant Cell. 15, 271-284.
Chew, W., Hrmova, M., Lopato, S., 2013. Role of homeodomain leucine zipper (HD-ZIP) IV transcription factors in plant development and plant protection from deleterious environmental factors. International Journal of Molecular Sciences. 14, 8122-8147.
Choi, H., Hong, J., Ha, J., Kang, J. and Kim, S.Y., 2000. ABFs, a family of ABA-responsive element binding factors. Journal of Biological Chemistry.21, 1723–1730.
Darvishzadeh, R., Pirzad, A., Hatami Maleki, H., Poormohammad Kiani S., Sarrafi A. 2010. Evaluation of the reaction of sunflower inbred lines and their F1 hybrids to drought conditions using various stress tolerance indices. Spanish Journal of Agricultural Research. 8, 1037-1046.
Darvishzadeh, R., Pirzad, A., Bernousi, I., Abdollahi Mandoulakani, B., Azizi, H., Akhondi, N., Poormohammad Kiani S., Sarrafi A. 2011. Genetic properties of drought tolerance indices in sunflower, Acta Agriculturae Scandinavica, Section B - Soil & Plant Science. 61, 593-601.
Darvishzadeh, R., Hewezi, T., Gentzbittel, L., Sarrafi, A., 2008. Differential expression of defence-related genes between compatible and partially compatible sunflower-Phoma macdonaldii interactions. Crop Protection. 27, 740-746.
Dynowski, M., Schaaf, G., Loque, D., 2008. Plant plasma membrane water channels conduct the signaling molecule H2O2. Journal of Biochemical and Pharmacological Research, 414, 53-61.
Esmailzadeh Mianlengeh, Z., Soltani Najafabadi, M., Saidi, A., Askari H., 2018. Monitoring response of a few bZip transcription factors in response to osmotic stress in sunflower. Iranian Journal of Biotechnology. 16(2), e1422. [In Persian with English summary].
FAO. 2018. Food and Agriculture Organization of the United Nations (FAO), Rome, Italy. Available from: http://www.faostat.fao.org/.
Gao, M. J., Allard, G., Byass, L., Flanagan, A. M., Singh, J., 2002. Regulation and characterization of four CBF transcription factors from Brassica napus. Plant Molecular Biology. 49, 459-471.
Giacomelli, J. I., Ribichich, K. F., Dezar, C. A., Chan, R. L., 2010. Expression analyses indicate the involvement of sunflower WRKY transcription factors in stress responses, and phylogenetic reconstructions reveal the existence of a novel clade in the Asteraceae. Plant Science. 178(4), 398-410.
Hasanuzzaman, M., Mahabub Alam, M., Nahar, K., Al-Mahmud, J, K., Fujita, M., 2014. Exogenous Salicylic acid alleviates salt stress-induced oxidative damage in Brassica napus by enhancing the antioxidant defense and glyoxalase systems. Australian Journal of Crop Science. 8, 631-639.
Hasanuzzaman, M. Fujita, M., 2013. Exogenous sodium nitroprusside alleviates arsenic-induced oxidative stress in wheat (Triticum aestivum L.) seedling by enhancing antioxidant defense and glyoxalase system. Ecotoxicology. 22, 584-596.
Heidarvand, L., Amiri Maali, R., 2013. Physiobiochemical and proteome analysis of chickpea in early phases of cold stress. Journal of Plant Physiology. 170, 459-469.
Hirayama, T. Shinozaki, K., 2010. Research on plant abiotic stress responses in the post-genome era: past, present and future. The Plant Journal. 61, 1041–1052.
Hossain, M.A., Cho, J.-I., Han, M., Ahn, C.-H., Jeon, J.-S., An, G., Park, P.B., 2010. The ABRE-binding bZIP transcription factor OsABF2 is a positive regulator of abiotic stress and ABA signaling in rice. Journal of Plant Physiology. 167, 1512–1520.
Hsieh, T.H., Li, C.W., Su, R.C., Cheng, C.P., Sanjaya Tsai, Y.C., Chan, M.T., 2010. A tomato bZIP transcription factor, SlAREB, is involved in water deficit and salt stress response. Planta. 231, 1459-1473.
Janmohammadi, M. Mahfoozi, S., 2013. Regulatory network of gene expression during the development of frost tolerance in plants. Current Opinion in Agriculture. 2, 11-19.
Javelle, M., Klein-Cosson, C., Vernoud, V., Boltz, V., Maher, C., Timmermans, M., Rogowsky, P., 2011. Genome-wide characterisation of the HD-ZIP IV transcription factor family in maize: preferential expression in the epidermis. Plant Physiology. 157, 790-80
Kobayashi, F., Takumi, S., Nakamura, C., 2008. Increased freezing tolerance in an ABA-hypersensitive mutant of common wheat. Journal of Plant Physiology. 165, 224-32
Lohar, D.P., Haridas, S., Gantt, J.S., 2007. A transient decrease in reactive oxygen species in roots leads to root hair deformation in the legume-rhizobia symbiosis. New Phytologist. 173, 39-49.
Madhava Rao, K.V, Raghavendra, A.S., Janardhan Reddy, K. 2006. Physiology and Molecular Biology of Stress Tolerance in Plants. Springer Netherlands.
Mare, C., Mazzucotelli, E., Crosatti, C., Francia, E., Cattivelli, L., 2004. Hv-WRKY38: a new transcription factor involved in cold-and drought-response in barley. Plant Molecular Biology. 55, 399-416.
Mohamadian Farsani, A., Hatami Maleki. H., Darvishzadeh, R., Hoshyardel, F., 2014. Study of changes in the phenylalanine ammonia-lyase 2 (PAL2) and thaumatin-like protein (TLP) genes in sunflower genotypes in response to fungal isolates associated with the black stem disease. Crop Biotechnology. 7, 15-23. [In Persian].
Muñiz García, M. N., País, S. M., Téllez-Iñón, M. T., Capiati, D. A., 2011. Characterization of StPPI1, a proton pump interactor from Solanum tuberosum L. that is up-regulated during tuber development and by abiotic stress. Planta. 233, 661-674.
Novillo, F., Alonso, J. M., Ecker, J. R., Salinas, J., 2004. CBF2/DREB1C is a negative regulator of CBF1/DREB1B and CBF3/DREB1A expression and plays a central role in stress tolerance in Arabidopsis. Proceedings of the National Academy of Sciences of USA. 101, 3985-3990.
Orellana, S., Yanez, M., Espinoza, A., Verdugo, I., Gonzalez, E., Ruiz-Lara, S., Casaretto J. A., 2010. The transcription factor SlAREB1 confers drought, salt stress tolerance, and regulates biotic and abiotic stress-related genes in tomato. Plant Cell and Environment. 33, 2191-2208.
Pffafi, M. W., 2001. A new mathematical model for relative quantification in real-time RT–PCR. Nucleic Acids Research. 29, e45-e45.
Poormohammad Kiani, S., Grieu, P., Maury, P., Hewezi, T., Gentzbittel, L., and Sarrafi, A. 2007. Genetic variability for physiological traits under drought conditions and differential expression of water stress-associated genes in sunflower (Helianthus annuus L.). Theoretical and Applied Genetics. 114, 193-207.
Qiu, Y. Yu, D., 2009. Over-expression of the stress-inducedOsWRKY45 enhances disease resistance and drought tolerance in Arabidopsis. Environmental and Experimental Botany. 65: 35-47.
Rabbani, M. A., Maruyama, K., Abe, H., Khan, M. A., Katsura, K., Ito, Y., Yamaguchi-Shinozaki, K., 2003. Monitoring expression profiles of rice genes under cold, drought, and high-salinity stresses and abscisic acid application using cDNA microarray and RNA gel-blot analyses. Plant Physiology. 133, 1755-1767.
Rahaii, M., Gomarian, M., Alizadeh, H., Melbobi, M. A., Naghavi, M. R., 2012. The expression analysis of transcription factors under long term salt stress in tolerant and susceptible wheat genotypes using reverse northern blot technique. Iranian Journal of Crop Sciences. 13, 580-595. [In Persian with English summary].
Riechmann, J. L., Heard, J., Martin, G., Reuber, L., Jiang, C. Z., Keddie, J., Creelman, R., 2000. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science. 290, 2105-2110.
Seki, M., Ishida, J., Narusaka, M., Fujita, M., Nanjo, T., Umezawa, T., Kamiya, A., Nakajima, M., Enju, A., Sakurai, T., Satou, M., Akiyama, K., Yamaguchi-Shinozaki, K., Carninci, P., Kawai, J., Hayashizaki, Y., Shinozaki, K., 2002. Monitoring the expression pattern of around 7,000 Arabidopsis genes under ABA treatments using a full-length cDNA microarray. Funct. Integr. Genomics. 2, 282–291.
Xiong, X., 2010. Constitutive expression of the barley HvWRKY38 transcription factor enhances drought tolerance in turf and forage grass (Paspalum notatum Flugge). Molecular Breeding. 25, 419-432.
Shah, D.A. Madden, L.V., 2004. Nonparametric analysis of ordinal data in designed factorial experiment. Phytopathology. 94, 33-43.
Shinozaki, K. Yamaguchi-Shinozaki, K., 2007. Gene networks involved in drought stress response and tolerance. Journal of Experimental Botany. 58, 221-7.
Soleimani, G.A., Darvishzadeh, R., Ebrahimi, A., Bihamta, M.R., 2018. Identification of SSR and retrotransposon-based molecular markers linked to morphological characters in oily sunflower under natural and water-limited states. Journal of Genetics. 97, 189-203.
Uno, Y., Furihata, T., Abe, H., Yoshida, R., Shinozaki, K., Yamaguchi-Shinozaki, K., 2000. Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions. Proceedings of the National Academy of Sciences of the United States of America. 97, 11632-11637.
Wang, Z., Zhu, Y., Wang, L., Liu, X., Liu, Y., Phillips, J., Deng, X., 2009. A WRKY transcription factor participates in dehydration tolerance in Boea hygrometrica by binding to the W-box elements of the galactinol synthase (BhGolS1) promoter. Planta. 230, 1155–1166.
Wang, H. H., Hao, J. J., Chen, X. J., Hao, Z. N., Wang, X., Lou, Y. G., Peng, Y. L., Guo, Z. J., 2007. Overexpression of rice WRKY89 enhances ultraviolet B tolerance and disease resistance in rice plants. Plant Molecular Biology. 65, 799–815
Yamaguchi-Shinozaki, K., Shinozaki, K., 2006. Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. Annual Review Plant Biology. 57, 781-803.
Zhang, G., Chen, M., Li, L., Xu, Z., Chen, X., Guo, J., Ma, Y., 2009. Overexpression of the soybean GmERF3 gene, an AP2/ERF type transcription factor for increased tolerances to salt, drought, and diseases in transgenic tobacco. Journal of Experimental Botany. 60, 3781-3796.