نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی سابق و فارغالتحصیل کارشناسی ارشد رشته اصلاح نباتات دانشگاه تهران
2 دانشیار گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران
3 استادیار گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران
چکیده
تنش خشکی یکی از مخرب‌ترین تنش‌های غیر زنده محسوب می‌شود. اکسپنسین‌ها پروتئین های بسط دهنده دیواره سلولی هستند که قادرند دیواره سلولی را تحت روشی که وابسته به pH می‌باشد، گسترش دهند. در این مطالعه نسل دوم (T2) گیاهان توتون تراریخت شده با ژن AtEXPA1 در سه لاین تراریخته 2، 4 و 7 و رقم تجاری سامسون به عنوان شاهد از نظر برخی صفات فیزیولوژیکی و بیوشیمیایی تحت تنش خشکی بررسی شدند. تنش خشکی باعث کاهش صفات محتوای نسبی آب برگ (RWCL) و میزان کلروفیل برگ و همچنین افزایش صفات شاخص هدایت الکتریکی برگ (ELIL)، پراکسیداسیون لیپیدهای غشایی برگ (MDAL) و میزان پرولین برگ شد. با اعمال تنش و افزایش سطوح تنشی، میزان فعالیت آنزیم‌های کاتالاز (CAT)، گایاکول پراکسیداز (GPOX)، آسکوربات پراکسیداز (APOX) و پلی فنل اکسیداز (PPO) در برگ افزایش یافت. در این مطالعه بر اساس صفات بیوشیمیایی، لاین 2 و 7 و بر اساس صفات فیزیولوژیکی، لاین 4 در مقایسه با رقم شاهد توانسته‌اند با تنش مقابله کنند و نسبت به تنش خشکی رقم متحمل محسوب شوند.
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