تجزیه‌وتحلیل بیان برخی از ژن‌های درگیر در شبکه های پیام رسانی در گیاه آلوروپوس ‌لیتورالیس (. Aeluropus littoralis (Gouan) Parl) تحت تنش شوری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری بیوتکنولوژی گیاهی، پژوهشکده ژنتیک و زیست فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری

2 استاد گروه زراعت و اصلاح نباتات، دکترای ژنتیک، دانشگاه علوم کشاورزی و منابع طبیعی ساری

3 پژوهشکده ژنتیک و زیست فناوری کشاورزی طبرستان، دکترای بیوتکنولوژی کشاورزی، دانشگاه علوم کشاورزی و منابع طبیعی ساری

چکیده

در این مطالعه، فعالیت آنتی­اکسیدانی و تغییرات بیان ژن­های مرتبط با شبکه پیام­رسان و تنظیمی پاسخ‌دهنده به تنش شوری شامل G-types-LecRLK، CIPK20، HSFA1a و C3H-ZF در برگ­های گیاه هالوفیت Aeluropus littoralis تحت تنش شوری مورد بررسی قرار گرفت. قلمه­ های گیاهی در شرایط هیدروپونیک کشت و پس از یک ماه تحت تیمارهای کنترل (محلول هوگلند بدون افزودن کلریدسدیم)، 200 و 400 میلی­ مولار نمک کلریدسدیم قرار گرفتند. پس از 72 ساعت تیمار، نمونه­ های برگی از گلدان­ ها جمع­ آوری شد و محتوای پراکسیدهیدروژن و فعالیت آنزیم آسکوربات­ پراکسیداز اندازه­ گیری و تغییرات بیان ژن­ های ذکرشده با استفاده از روش qRT-PCR ارزیابی شد. نتایج نشان داد که فعالیت آنزیم آسکوربات­ پراکسیداز در هر دو غلظت 200 و 400 میلی ­مولار به‌طور معنی­ داری افزایش یافت (0.05>P). بیان ژن LecRLK تغییر چندانی را نسبت به شاهد نشان نداد و بیان ژن CIPK20 به ترتیب 7 و 16 برابر نسبت به شاهد در هر دو تیمار 200 و 400 میلی ­مولار کلریدسدیم کاهش یافت. بیان ژن HSFA1a با مقدار نمک رابطه مثبت داشت (0.05>P)  و با افزایش غلظت نمک افزایش چشمگیری نشان داد. بیان ژن C3H-ZF به‌طور معنی­ داری در هر دو تیمار 200 و 400 میلی­ مولار نسبت به شاهد کاهش یافت (0.05>P). نتایج نشان داد که الگوی بیان ژن­های موردمطالعه تحت شرایط تنش شوری می­تواند به نقش هر ژن، غلظت نمک و مدت‌زمان تنش مربوط باشد.

کلیدواژه‌ها


 
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