تنش‌های محیطی در علوم زراعی

تنش‌های محیطی در علوم زراعی

تأثیر پیش‌تیمار بذر با ملاتونین بر جوانه‌زنی، رشد، شاخص‌های بیوشیمیایی و متابولیت‌های ثانویه گیاهچه‌های گل همیشه بهار (.LCalendula officinalis )تحت تنش کادمیوم

مقالات آماده انتشار

نوع مقاله : مقاله پژوهشی

نویسندگان
بهروز اسماعیل پور 1
فاطمه محمودی 2
زهرا مهدوی 3
پریسا شیخ زاده 4
1 استاد، گروه علوم باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل
2 دکتری علوم و تکنولوژی بذر، گروه تولید و ژنتیک گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل
3 گروه علوم باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل
4 دانشیار، گروه تولید و ژنتیک گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل
10.22077/escs.2026.10570.2426
چکیده
این پژوهش با هدف بررسی تأثیر پیش‌تیمار بذر با ملاتونین بر جوانه‌زنی، رشد، شاخص‌های بیوشیمیایی و برخی متابولیت‌های ثانویه گیاهچه‌ی همیشه‌بهار (Calendula officinalis L.) تحت تنش کادمیوم، به‌صورت فاکتوریل در قالب طرح پایه‌ کاملاً تصادفی در سه تکرار در دانشگاه محقق اردبیلی، دانشکده کشاورزی و منابع طبیعی، بصورت مشترک بین گروه مهندسی علوم باغبانی و گروه تولید و ژنتیک گیاهی در آزمایشگاه علوم و تکنولوژی بذر در اسفند سال 1403 انجام شد. عامل‌های آزمایش شامل تنش کادمیوم در سه سطح (صفر (عدم اعمال کادمیوم)، 100 و 300 میلی‌گرم در لیتر کادمیوم)، غلظت‌های ملاتونین در چهار سطح (شاهد، 50، 100 و 150 میکرومولار) بودند. نتایج نشان داد تنش کادمیوم موجب کاهش درصد و سرعت جوانه‌زنی، رشد گیاهچه و فعالیت آنتی‌اکسیدانی شد. پیش‌تیمار بذر با ملاتونین در غلظت ۱۵۰ میکرومولار، موجب بهبود جوانه‌زنی و رشد گیاهچه، افزایش فعالیت آنزیم‌های آنتی‌اکسیدانی (کاتالاز، پراکسیداز و پلی‌فنل‌اکسیداز) و افزایش محتوای ترکیبات فنولی و فلاونوئیدی گردید. پیش‌تیمار بذر با ملاتونین توانست با تقویت سیستم آنتی‌اکسیدانی و افزایش ترکیبات متابولیت‌های ثانویه، اثرات منفی تنش کادمیوم را کاهش داده و موجب بهبود عملکرد فیزیولوژیک گیاهچه‌های همیشه‌بهار شد. بنابراین، استفاده از ملاتونین می‌تواند راهکاری مؤثر برای افزایش تحمل گیاهان دارویی به تنش فلزات سنگین باشد. در مجموع، پیش‌تیمار بذر با 150 میکرومولار ملاتونین، در سطوح مختلف تنش کادمیوم از راه افزایش توان بذر و گیاهچه و افزایش فعالیت آنزیم‌های آنتی اکسیدانی بیشترین اثر مثبت را بر جوانه‌زنی و رشد گیاهچه‌های همیشه بهار داشت.
کلیدواژه‌ها
آنزیم‌های آنتی­ اکسیدان
پیش‌تیمار بذر
مالون دی آلدهید
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