Environmental Stresses in Crop Sciences

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The effects of foliar application of humic acid and garlic extract on nutrients uptake (NPK) and growth of wheat under drought stress conditions

Document Type : Original Article

Authors

1 MSc. Student, Department of Soil Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran

2 Associate Professor, Department of Soil Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran

3 Professor, Department of Soil Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran

Abstract

Introduction
With the approach of attention to the environment, the use of natural materials can be effective in reducing the harmful effects of chemical fertilizers and artificial growth regulators. Drought stress is one of the main limitations of food production around the world, and due to increasing population and water deficiency, there is a global attention for using some materials to increase resistant of plant to drought stress. Chemical stimulants are currently used for decreasing negative effects of water stress in plant. Now a days, use of natural materials, such as plant extract is recommended by researchers. Garlic extract is one of the natural extracts that contains many enzymes and more than 200 biochemical compounds that, due to its richness in minerals and plant hormones, causes cell enlargement, cell division and, plant growth. Humic acid is created from the decomposition of organic matter and is beneficial to plant growth and development. Therefore, the aims of this research were to study the effects of using natural substances of humic acid and garlic extract on nutrients uptake (NPK) and growth of wheat plant under drought stress.
Materials and methods
This study was performed during 2018 in a completely randomized design with factorial arrangement in three replications under greenhouse conditions. The studied soil with texture class of loam had pH = 8.11 and EC = 1.28 dS m-1. Experimental factors include drought stress at three levels of 95%, 65% and 45% of field capacity, garlic extract at three levels of foliar application of 0, 2% and 4% and humic acid at two levels of foliar application of 0 and 300 mg l-1. Drought stress were treated by the weight method, 17 days after sowing. Foliar application of humic acid (twice, 13 and 20 days after threating the drought stress) and garlic extract (three times, 18, 24 and 27 days after threating the drought stress) were performed by spraying 150 ml pot-1 of the solutions in each time. The plants were harvested 54 days after sowing and the parameters of shoot dry weight and uptake of nitrogen, phosphorus and potassium in plant were measured. Statistical analysis was done using SAS 9.4 software and the comparison of means was done using Tukey's test at the 5% probability level.
Results and discussion
The results showed that foliar application of humic acid and garlic extract alone had a positive effect on dry weight and uptake of nitrogen and phosphorus of shoot. In addition, garlic extract also increased the potassium uptake. Drought stress reduced all investigated parameters; however, foliar application of garlic extract under drought stress conditions increased the absorption of nitrogen, phosphorus, and potassium. In 65% of field capacity, nitrogen uptake by using 2% garlic extract and phosphorus and potassium uptake by using 4% garlic extract had a maximum amount compared to others. In general, garlic extract, due to its antioxidant properties and the presence of growth-promoting compounds such as minerals, vitamins, phytohormones, sulfur compounds, enzymes and amino acids, can improve growth parameters and absorption of nutrients in wheat plant. Humic acid improved growth parameters due to its role in increasing cell membrane permeability, respiration and photosynthesis, phosphate absorption, root and cell expansion, and acting as hormone-like substances.
Conclusion
The foliar application of wheat plant with garlic extract and humic acid as natural stimulants had a favorable effect on the plant growth and nutrients uptake. Garlic extract was more effective than humic acid in reducing the negative effects of drought stress. Finally, the use of natural materials such as garlic extract and some high consumption compounds such as humic acid can be an environment-friendly and safe approach to improve plant growth and to reduce the destructive effects of chemical fertilizers and synthetic growth regulators.
 

Keywords

Main Subjects

References
 
 Abd-Elhamied, A.S., 2017. The influence of the foliar application of urea, licorice and garlic extract on nutrients uptake and yield of wheat plants grown on sandy soil. A comparative study. Journal of Soil Sciences and Agricultural Engineering. 8, 157-162. https://doi.org/10.21608/jssae.2017.37236
Abd El-Hamied, S.A., El-Amary, E.I., 2015. Improving growth and productivity of “Pear” trees using some natural plants extracts under north sinai conditions. Journal of Agriculture and veterinary Science. 8, 1-9. https://doi.org/10.9790/2380-08110109
Abdou, M.A., El-Sayed, A.A., Taha, R.A.I., Abd-El Sayed, M.A., Botros, W.S.E., 2017. Effect of compost and some biostimulant treatments on guar plants. A-Vegetative growth and seed yield. Scientific Journal of Flowers and Ornamental Plants. 4, 143-157. https://doi.org/10.21608/sjfop.2017.5401
Abdul Jaleel, C., Gopi, R., Panneerselvam, R., 2009. Alterations in non-enzymatic antioxidant components of Catharanthus roseus exposed to paclobutrazol, gibberellic acid and Pseudomonas fluorescens. Plant Omics Journal. 2, 30-40.
Baque, M.A., Karim, M.A., Hamid, A., Tetsushi, H., 2006. Effects of fertilizer potassium on growth, yield and nutrient uptake of wheat (Triticum aestivum) under water stress conditions. South Pacific Studies. 27, 25-35.
Barnabás, B., Jäger, K., Fehér, A., 2008. The effect of drought and heat stress on reproductive processes in cereals. Plant, Cell and Environment. 31, 11-38. https://doi.org/10.1111/j.1365-3040.2007.01727.x
Bhandari, P.R., 2012. Garlic (Allium sativum L.): A review of potential therapeutic applications. International Journal of Green Pharmacy (IJGP). 6, 118-129.
Bremner, J.M., Mulvaney, C.S., 1982. Nitrogen—Total. In: Page, A.L. (2nd eds.), Methods of Soil Analysis: Part 2, Chemical and Microbiological Properties. American Society of Agronomy and Soil Science Society of America, Madison, WI 53711, USA, pp. 595-624. https://doi.org/10.2134/agronmonogr9.2.2ed.c31
Cardelle-Cobas, A., Soria, A.C., Corzo-Martínez, M., Villamiel, M., 2010. A comprehensive survey of garlic functionality. In: Pacurar, M., Krejci, G. (eds.), Garlic Consumption and Health. Garlic Consumption and Health, Nova Science Publishers, pp. 1-60. https://doi.org/10.13140/2.1.4992.6728
El-Azizy, F.A., 2021. Effect of different foliar concentration of some bio-stimulators on egyptian clover in calcareous soil. Egyptian Journal of Applied Sciences. 36, 94-112. https://doi.org/10.21608/ejas.2021.163553
El-Bably, S.M.Z., 2017. Effect of some natural material additives on growth and flowering of tuberose (Polianthes tuberosa, L.) bulbs. Journal of Plant Production. 8, 895-906. https://doi.org/10.21608/jpp.2017.40897
El-Bassiouny, H.S.M., Bakry, B.A., Attia, A.A.E.M., Abd Allah, M.M., 2014. Physiological role of humic acid and nicotinamide on improving plant growth, yield, and mineral nutrient of wheat (Triticum durum) grown under newly reclaimed sandy soil. Agricultural Sciences. 5, 687-700. https://doi.org/10.4236/as.2014.58072
El-Desouky, S.A., Wanas, A.L.A., Khedr, Z.M.A., 1998. Utilization of some natural plant extracts (garlic and yeast) as seed-soaked materials to squash (Cucurbita pepo, L.). 1-Effect on growth, sex expression and fruit yield and quality. Annals of Agricultural Science Moshtohor. 36, 839-854.
El-Saadony, F.M., Mohsen, A.A.M., Inas, A.B., 2019. Effect of foliar spray with some plant natural extracts on growth and yield of (Phaseolus vulgaris L.) plants. Fayoum Journal of Agricultural Research and Development. 33, 66-77. https://doi.org/10.21608/fjard.2019.190154
El-Saadony, F.M., Nawar, D.A.S., Zyada, H.G., 2017. Effect of foliar application with salicylic acid, garlic extract and proline on growth, yield and leaf anatomy of pea (Pisum sativum L.) grown under drought stress. Middle East Journal of Applied Sciences. 7, 633-650.
El-Said, N.A.M., Ali, H.M.H., 2013. Physiological effects of garlic aqueous extract on cumin. Egyptian Journal of Horticulture. 40, 247-260. https://doi.org/10.21608/ejoh.2013.1347
El Tayeb, M.A., Ahmed, N.L., 2010. Response of wheat cultivars to drought and salicylic acid. American-Eurasian Journal of Agronomy. 3, 1-7.
Elshaboury, H.A., Sakara, H.M., 2021. The role of garlic and onion extracts in growth and productivity of onion under soil application of potassium humate and fulvate. Egyptian Journal of Soil Science. 61, 161-170. https://doi.org/10.21608/ejss.2021.64114.1434
Elzaawely, A.A., Ahmed, M.E., Maswada, H.F., Al-Araby, A.A., Xuan, T.D., 2018. Growth traits, physiological parameters and hormonal status of snap bean (Phaseolus vulgaris L.) sprayed with garlic cloves extract. Archives of Agronomy and Soil Science. 64, 1068-1082. https://doi.org/10.1080/03650340.2017.1410543
Estefan, G., Sommer, R., Ryan, J., 2013. Methods of Soil, Plant, and Water Analysis: A manual for the West Asia and North Africa region. (3rd eds.). International Center for Agricultural Research in the Dry Areas (ICARDA), Beirut, Lebanon.
Ferrara, G., Brunetti, G., 2010. Effects of the times of application of a soil humic acid on berry quality of table grape (Vitis vinifera L.) cv Italia. Spanish Journal of Agricultural Research. 8, 817-822. https://doi.org/10.5424/1283
Fouda, K.F., 2021. Effect of foliar application of humic acid, Em and mineral fertilization on yield and quality of carrot under organic fertilization. Journal of Soil Sciences and Agricultural Engineering. 12, 1-7. https://doi.org/10.21608/jssae.2021.152002
Gee, G.W., Bauder, J.W., 1986. Particle-size analysis In: Klute, A. (2nd eds.), Methods of Soil Analysis: Part 1-Physical and Mineralogical Methods. American Society of Agronomy, Soil Science Society of America, Madison, Winsconsin, USA, pp. 383-411. https://doi.org/10.2136/sssabookser5.1.2ed.c15
Hammad, S.A., 2008. Physiological and anatomical studies on drought tolerance of pea plants by application of some natural extracts. Annals of Agricultural Science (Cairo). 53, 285-305.
Hasanuzzaman, M., Fujita, M., 2011. Selenium pretreatment upregulates the antioxidant defense and methylglyoxal detoxification system and confers enhanced tolerance to drought stress in rapeseed seedlings. Biological Trace Element Research. 143, 1758-1776. https://doi.org/10.1007/s12011-011-8998-9
Hawkesford, M., Horst, W., Kichey, T., Lambers, H., Schjoerring, J., Møller, I.S., White, P., 2012. Functions of macronutrients. In: Marschner, P. (3rd eds.), Marschner's Mineral Nutrition of Higher Plants. Academic press, pp. 135-189. https://doi.org/10.1016/B978-0-12-384905-2.00006-6
Hayat, S., Ahmad, H., Ren, K., Ali, M., Cheng, Z., 2018. Response of tomato growth to foliar spray and root drenching of aqueous garlic extract: A cocktail of antioxidative defenses, chlorophyll, carotenoid and soluble sugar contents. International Journal of Agriculture and Biology. 20, 1251-1259.
Helmke, P.A., Sparks, D.L., 1996. Lithium, Sodium, Potassium, Rubidium, and Cesium. In: Sparks, D.L., Page, A.L., Helmke, P.A., Loeppert, R.H., Soltanpour, P.N., Tabatabai, M.A., Johnston, C.T., Sumner, M.E. (eds.), Methods of Soil Analysis: Part 3, Chemical Methods. Soil Science Society of America and American Society of Agronomy, Madison, WI 53711, USA, pp. 551-574. https://doi.org/10.2136/sssabookser5.3.c19
Hu, Y., Schmidhalter, U., 2005. Drought and salinity: A comparison of their effects on mineral nutrition of plants. Journal of Plant Nutrition and Soil Science. 168, 541-549. https://doi.org/10.1002/jpln.200420516
Karakurt, Y., Unlu, H., Unlu, H., Padem, H., 2009. The influence of foliar and soil fertilization of humic acid on yield and quality of pepper. Acta Agriculturae Scandinavica Section B–Soil and Plant Science. 59, 233-237. https://doi.org/10.1080/09064710802022952
Kasim, W.A., Nessem, A.A., Gaber, A. 2017. Alleviation of drought stress in Vicia faba by seed priming with ascorbic acid or extracts of garlic and carrot. p. 45-59. In: Proceedings of the 7th International Conference “Plant and Microbial Biotechnology and their Role in the Development of the Society”, October 2017. Egyptian Journal of Botany. Egypt. https://doi.org/10.21608/ejbo.2017.831.1057
Khaled, H., Fawy, H.A., 2011. Effect of different levels of humic acids on the nutrient content, plant growth, and soil properties under conditions of salinity. Soil and Water Research. 6, 21-29. https://doi.org/10.17221/4/2010-SWR
Loeppert, R.H., Suarez, D.L., 1996. Carbonate and gypsum. In: Sparks, D.L., Page, A.L., Helmke, P.A., Loeppert, R.H., Soltanpour, P.N., Tabatabai, M.A., Johnston, C.T., Sumner, M.E. (eds.), Methods of Soil Analysis: Part 3, Chemical Methods. Soil Science Society of America and American Society of Agronomy, Madison, WI 53711, USA, pp. 437-474. https://doi.org/10.2136/sssabookser5.3.c15
Mahmood, Y.A., Ahmed, F.W., Juma, S.S., Al-Arazah, A.A., 2019. Effect of solid and liquid organic fertilizer and spray with humic acid and nutrient uptake of nitrogen, phosphorus and potassium on growth, yield of cauliflower. Plant Archives. 19, 1504-1509.
Mardomi, R., 2017. Determining the chemical compositions of garlic plant and its existing active element. IOSR Journal of Applied Chemistry. 10, 63-66. https://doi.org/10.9790/5736-1001016366
Massoud, H.Y., Kasem, M.M., El-Banna, M.F., Farag, N.B.B., 2020. Effect of garlic, moringa and licorice extracts on vegetative growth and chemical constituents of Zanthoxylum beecheyanum K. koch plant. Journal of Plant Production. 11, 465-472. https://doi.org/10.21608/jpp.2020.102770
Mohamed, H.I., Akladious, S.A., 2014. Influence of garlic extract on enzymatic and non enzymatic antioxidants in soybean plants (Glycine max) grown under drought stress. Life Science Journal. 11(3s), 46-58.
Mohamed, M.H., Badr, E.A., Sadak, M.S., Khedr, H.H., 2020. Effect of garlic extract, ascorbic acid and nicotinamide on growth, some biochemical aspects, yield and its components of three faba bean (Vicia faba L.) cultivars under sandy soil conditions. Bulletin of the National Research Centre. 44, 1-8. https://doi.org/10.1186/s42269-020-00359-z
Olsen, S.R., Sommers, L.E., 1982. Phosphorus. In: Page, A.L. (2nd eds.), Methods of Soil Analysis: Part 2, Chemical and Microbiological Properties. American Society of Agronomy and Soil Science Society of America, Madison, WI 53711, USA, pp. 403-430. https://doi.org/10.2134/agronmonogr9.2.2ed.c24
Osakabe, Y., Osakabe, K., Shinozaki, K., Tran, L.S.P., 2014. Response of plants to water stress. Frontiers in plant science. 5, 1-8. https://doi.org/10.3389/fpls.2014.00086
Polyakov, A., Alekseeva, T., Muravieva, I. 2020. The elemental composition of garlic (Allium sativum L.) and its variability. p. 1-9. In: Proceedings of E3S Web of Conferences XIII International Scientific and Practical Conference “State and Prospects for the Development of Agribusiness, June 29. 2020. EDP Sciences. Russia. https://doi.org/10.1051/e3sconf/202017501016
Rhoades, J.D., 1996. Salinity: Electrical conductivity and total dissolved solids. In: Sparks, D.L., Page, A.L., Helmke, P.A., Loeppert, R.H., Soltanpour, P.N., Tabatabai, M.A., Johnston, C.T., Sumner, M.E. (eds.), Methods of Soil Analysis: Part 3, Chemical Methods. Soil Science Society of America and American Society of Agronomy, Madison, WI 53711, USA, pp. 417-435. https://doi.org/10.2136/sssabookser5.3.c14
Russo, R.O., Berlyn, G.P., 1991. The use of organic biostimulants to help low input sustainable agriculture. Journal of Sustainable Agriculture. 1, 19-42. https://doi.org/10.1300/J064v01n02_04
Sabzevari, S., Khazaei, H.R., 2009. The effect of foliar application with humic acid on growth, yield and yield components of wheat (Triticum aestivum L.). Journal of Agroecology. 1, 53-63. [In Persian].  https://doi.org/10.22067/jag.v1i2.2686
Shakir, M.A., Al-Rawi, W.A.A., 2017. Effect of garlic and licorice root extract on leaves mineral and hormonal content of pear transplants. The Iraqi Journal of Agricultural Science. 48, 138-143. https://doi.org/10.36103/ijas.v48iSpecial.254
Sparks, D.L., 2003. Environmental soil chemistry. In: (2nd eds.), Chemistry of Soil Organic Matter. Elsevier Science, USA, pp. 75-113. https://doi.org/10.1016/B978-0-12-656446-4.X5000-2
Thomas, G.W., 1996. Soil pH and soil acidity. In: Sparks, D.L., Page, A.L., Helmke, P.A., Loeppert, R.H., Soltanpour, P.N., Tabatabai, M.A., Johnston, C.T., Sumner, M.E. (eds.), Methods of Soil Analysis: Part 3, Chemical Methods. Soil Science Society of America and American Society of Agronomy, Madison, WI 53711, USA, pp. 475-490. https://doi.org/10.2136/sssabookser5.3.c16
Tohidi Moghadam, H.R., Khamene, M.K., Zahedi, H., 2014. Effect of humic acid foliar application on growth and quantity of corn in irrigation withholding at different growth stages. Maydica. 59, 124-128. https://doi.org/10.13140/RG.2.1.4147.1208
Tourfi, F., Shokuhfar, A., 2019. Effect of humic acid on yield, yield components and physiological parameters of wheat in deficit irrigation conditions. Quarterly Journal of Plant Production. 9, 121-132. [In Persian].
Türkan, I., Demiral, T., 2009. Recent developments in understanding salinity tolerance. Environmental and Experimental Botany. 67, 2-9. https://doi.org/10.1016/j.envexpbot.2009.05.008
Wafaa, H.A.A., Rania, M.R.K., El-Shafay, R.M.M., 2021. Effect of spraying with extracts of plants and amino acids on growth and productivity on Coriandrum sativum L. plants under shalateen condition. Plant Archives. 21, 300-307. https://doi.org/10.51470/PLANTARCHIVES.2021.v21.S1.048
Walkley, A., Black, I.A., 1934. An examination of the degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration method. Journal of Soil science. 37, 29-38. https://doi.org/10.1097/00010694-193401000-00003
Wanas, A.L., Khamis, M.A.I., 2021. Effect of garlic and licorice extracts on vegetative growth and leaf anatomy of strawberry plants cultivated in different growing media. Scientific Journal for Damietta Faculty of Science. 11, 89-102. https://doi.org/10.21608/sjdfs.2021.195598
Waraich, E.A., Ahmad, R., Saifullah, Ashraf, M.Y., Ehsanullah, 2011. Role of mineral nutrition in alleviation of drought stress in plants. Australian Journal of Crop Science. 5, 764-777.
Yadav, M., Choudhary, B., Gadwal, R., Singh, P., 2020. Humic substances: the black gold for agriculture. Krishi Science. 1, 27-29.
Ziedan, E.H., Eisa, E.A., 2016. The use of some micronutrients and plant extracts of resistance to powdery mildew and nutrition dill plants in the gharbiyah governorate. Journal of Plant Protection and Pathology. 7, 579-586. https://doi.org/10.21608/jppp.2016.52019  
 
Environmental Stresses in Crop Sciences
Volume 17, Issue 2
Open Access Policy
July 2024
Pages 371-385
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  • Receive Date: 22 November 2022
  • Revise Date: 02 February 2023
  • Accept Date: 05 February 2023
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