Document Type : Original Article

Authors

1 M.Sc. Student of Seed Science and Technology, Shahrekord University, Iran

2 Associate Professor of Agronomy Department, Shahrekord University, Iran

Abstract

Introduction
Water scarcity is one of the main problems in the agricultural land around the world. The climate changes indicate a rise in temperature and a decrease in average rainfall, which that its augments the drought stress effects in future (Farre and Faci, 2006). Tolerance to dehydration plants to grow in arid and salty is very important. Germination and establishment of plants due to low viability is difficult and environmental stresses, especially drought than other factors are reduction of green. Priming one of the ways to increase the germination under stress conditions. The aim of this study is to investigate the effect of seed priming treatments on emergence parameters improve of ajowan (Carum copticum L.) medicinal plant.
 
Material and methods
A split plot experiment was conducted in randomized complete block design with three replications at farm of agricultural college, Shahrekord University, 2014. Different levels of irrigation (irrigation after 11, 22, and 33 mm evaporation from pan evaporation class A) were evaluated as the main plot and seed priming treatments consisted of no priming (control), hydropriming (seed soaking in distilled water for 36 hours), KNO3 (seeds were placed on potassium nitrate 4%), ZnSO4 (seeds were placed on potassium nitrate 0.1%), PEG600 (seeds were placed on -12 bar of polyethylene glycol) and GA3 (seeds were placed on 100 ppm of gibberellic acid) as subplot. Treatments were chosen according to a preliminary priming experiment. The amount of potassium nitrate required is calculated using equation Want hoof (Siebert and Richardson, 2002). In this experiment, parameters such as rate of emergence, percentage of emergence, root dry weight, shoot dry weight, root length, shoot length, shoot dry weight, allometric coefficient, and vigor index were evaluated. data were analyzed by using both one- or two-way analysis of variance (ANOVA) and mean comparisons were performed by LSD test if F-test was significant at (p < 0.05) to determine whether differences among means were significant between treatments drought stress and seed priming.
 
Results and discussion
The results showed that emergence percentage, root dry weight, soot dry weight, vigor index were influenced by drought stress (p < 0.05, p < 0.01, and p < 0.01, respectively). The seed priming effect for emergence rate, emergence percentage, root dry weight, shoot dry weight, root length, shoot length, shoot dry weight, allometric coefficient, and vigor index was significant (p < 0.01, p < 0.01, p < 0.01, p < 0.05, p < 0.01, p < 0.01, p < 0.01, p < 0.01, and p < 0.01, respectively). The drought stress × seed priming interactions for emergence rate, emergence percentage, root dry weight, shoot dry weight, root length, shoot length, shoot dry weight, allometric coefficient, and vigor index was significant were statistically significant. Means comparison results indicated that in mild stress (irrigation after 22 mm evaporation), the highest rate and percentage of emergence were obtained in PEG treatment. The greatest of root dry weight was observed in GA3, KNO3, and ZnSO4, and PEG treatments. In addition, root length, shoot length, shoot dry weight, allometric coefficient of primed seed not only not improved but also values of these characters declined in compare to non-primed seed. In severe stress (irrigation after 33 mm evaporation), rate and percentage of emergence and vigor index increased in PEG, GA3, ZnSO4. Also, The maximum root length (6.7 cm) and shoot length (4.4 cm), were observed GA3 and ZnSO4, respectively.Primed seed with ZnSO4 increased shoot dry weight (16.12mg) and the allometric coefficient (0.82), as well as.
 
Conclusions
In overall it can be concluded that in drought stress conditions, seed priming of ajowan medicinal plant with gibberellic acid and zinc sulfate improves germination and seedling growth in farming systems. Thus, these pretreatments increases root growth and strengthen and better establishment seedling under drought stress conditions that it would be appropriate for the development of production in such semi-arid environments. In addition, by reducing the frequency of irrigation for optimum seedling establishment can make good use of water resources in these areas.

Keywords

Ansari, O., Sharifzadeh F., 2012. Osmo and hydro priming mediated germination improvment under cold stress conditions in mountain rye (Secale montanum). Cercetări Agronomice în Moldova. 3(151), 53-6
Basra, S.M.A., Ashraf, M., Iqbal, N., Khaliq, A., Ahmad, R., 2004. Physiological and biochemical aspect of pre-sowing heat stress on cotton seed. Seed Science and Technology. 32, 765-774.
Bybordi, A., Tabatabaei, J., 2009. Effect of salinity stress on germination and seedling properties in canola cultivar (Brassica napus L.). Notulae Botanicae, Horti Agrobotanici, Cluj-Napoca. 37(2), 71-76. [In Persian with English Summary].
Cakmak, I., 2008. Enrichment of cereal grains with zinc: agronomic or genetic biofortification. Plant and Soil. 302, 1-17.
Chana, S.G., Schillinger, W.F., 2003. Seed priming winter wheat for germination emergence and yield. Crop Science. 43, 2135-2141.
Chaves, M M J., S., Pereira, J., Maroco, M L., Rodrigues, C.P P., Ricardo, M.L., Osorio, I., Carvalho, T., Faria, Pinheiro, C., 2002. How plants cope with water stress in the field photosynthesis and growth. Annals of Botany. 89, 907-916.
Chen, K., Arora, R., and Arora, U., 2010. Osmopriming of Spinach (Spinacia oleracea L. CV. Bloomsdale) seeds and germination performance under temperature and water stress. Seed Science and Technology. 38, 36-48.
Christine, M.F., Tai-ping, S., 2005. A DELLAcate balance: the role of gibberellin in plant Morphogene. Current Opinion in Plant Biology. 8, 77-85.
Demir,r K.M., Gamze, O., Atak, M., Cikili, Y., Kolsarici, O., 2006. Seed treatment to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). European Journal of Agronomy. 24, 291-295.
Ellis, R.A., Roberts, E.H., 1981. The quantification of ageing and survival in orthodox seeds. Seed Science and Technology. 9, 373- 409.
 Farre, I., Faci, J.M., 2006. Comparative response of maize and sorghum to deficit irrigation in a Mediterranean environment. Agricultural Water Management. 83, 135-143.
Farre, I., Faci, J.M., 2009. Deficit irrigation in maize for reducing agricultural water use in a Mediterranean environment. Agricultural Water Management, 96, 383-394.
Fathi Amirkhizi, k., Omidi, H., Heshmati, S., Jafarzadeh, L., 2012. Catalytic effect of the medicinal plant vigor and germination characteristics of black cumin (Nigella sativa L.). Iranian Field Crop Research. 10, 310-299. [In Persian with English Summary].
Hanan, A.E.S., Mona, M.M., Hany, M.H., 2010. Biochemical and molecular profiles of gibberllic acid exposed Albino Rats. Journal of American Science. 6(8), 18-23
Harris, D., R.S., Tripathi Shi, A.J., 2000. On- farm seed priming to improve crop establishment and yield in direct- seeded rice in IRRI: International Workshop on Dry seeded Rice Technology, held in Bangkok 25-28 January International Rice Research Institute, Manila, Philippines, 164 pp.
Hasanzadeh Khankahdani, H., Mohammadi Jahromi, S.A., Zakerifard Mollahasani, E., Mohammadi Jahromi, M.S., 2014. The effect of irrigation on yield and yield components of four varieties of onion in hot and humid climates. Journal of Water Research in Agriculture. 27(2), 137-147. [In Persian with English Summary].
Ikic, I., Maric evic, M., Tomasovic, S., Gunjaca, J., Atovic, Z.S., Arcevic, H.S., 2012. The effect of germination temperature on seed dormancy in Croatian-grown winter wheats. Euphytica. 188, 25-34.
ISTA (International Seed Testing Association), 2009. International Rules for Seed Testing. International Seed Testing Association, Bassersdorf, Switzerland.
Jalil Shesh Bahre, M., Movahedi Dehnavi, M., 2011. Effect of zinc and ironfoliar application on soybesn seed vigor grown under drough stress. Iranian Socity of Agronomy and Plant Breading Sciences.. 5 (1), 19-35. [In Persian with English Summary].
Kalsa, K.K., Abebie, B., 2012. Influence of seed priming on seed germination and vigor traits of Vicia villosa ssp. dasycarpa (Ten.). African Journal of Agricultural Research. 7(21), 3202-3208.
 Majnon Husseini, M., Davazda Emami, S., 2007. Agriculture and some manufacturing plants and Advyhay - Tehran University Press. [In Persian].
Michel, B.E., Kaufmann, M.R., 1973. The osmotic potential of polyethylene glycol 6000. Plant Physiology. 51, 914-916.
Preece, J.E., Read, P.E., 1993. Mineral Nutrition. In the Biology of Horticulture Crop. 2ed. Jhon Wiley and Sons Publisher. 257-259.
Rion, B., Alloway, J., 2004. Fundamental aspects of zinc in soils plants. International Zinc Association. 23, 1-128.
Sasani, Sh, Tavakol Afshar, R., 2007. Effects of moist hormonal treatments and storage period on breaking dormancy and induce germination of caraway (Curum carvi L.). Iranian Journal of Agricultural Sciences 38 (2), 294-287. [In Persian with English Summary].
Scott, S.J., Jones, R.A., Williams, W.A., 1984. Review of data analysis method for seed germination. Crop Sciences. 24, 1192-1199.
Sharma, S., Sharma, P., Datta, S.P., and Gupta, V., 2009. Morphological and biochemical response of Cicer arietinum var. Pusa-256 towards an excess zinc concentration. African Journal of Basic and Applied Sciences, 1(5-6), 105-109.
Soltani, A., M., Coholipoor, Zeinali, E., 2006. Seed reserve utilization and seedling growth of wheat as affected by drought and salinity. Environmental and Experimental Botany. 55, 195-200. [In Persian with English Summary].
Varier, A., Vari, A.K., Dadlani, M., 2010. The subcellular basis of seed priming. Current Sciences. 99, 450-456.
Voigt, E.L., T.D., Almeida, R.M., Chagas, L.F.A., Ponte, R.A., viegas Silveira, J.G.A., 2009. Source–sink regulation of cotyledonary reserve mobilization during cashew (Anacardium occidentale) seedling establishment under NaCl salinity. Journal of Plant Physiology. 166, 80-89.
Windauer, l., Altuna, A., Benech- Arnold, F., 2007. Hydrotime analysis of Lesquerella fendleri seed germination responses to priming treatments. Industrial Crops and Products. 25, 70-74.
Ya-jing, G., Jin, H., Xian-ju, W., Chen-xia, S., 2009. Seed priming with chitosan improves maize germination and seedling growth in relation to physiological changes under low temperature stress. Journal of Zhejiang University Science B. 10(6), 427-433.
Yousefi tanha, A., 2014. Effect of seed priming treatments to improve germination of winter annual green manure seeds under cold stress. Senior Thesis seed science and technology. University of ShahreKord Iran. [In Persian with English Summary].