Document Type : Original Article

Authors

1 MSc of Soil Science, Sari Agricultural Sciences and Natural Resources University

2 Associate Professor of Soil Science Department, Sari Agricultural Sciences and Natural Resources University

3 Associate Research of Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran

Abstract

To evaluate the effect of drought stress on some morphological and physiological properties of Echium amoenum Fisch & Mey medicinal plant, an experiment was carried out in completely randomized design with four replications at the research greenhouse of Institute of Medicinal Plants of Iranian Academic Center for Education, Culture and Research (ACECR). In this experiment, the effect of different levels of drought stress including irrigation in field capacity as control treatment and irrigation after discharging 25, 50 and 75 percent of water on plant quantity characteristics (Leaf length and width, Root length, Root and Shoot fresh and dry weight) and quality characteristics (Proline and Total soluble sugar content) in vegetative growth level were investigated under greenhouse conditions. The results showed that drought stress caused a reduction in all morphological characteristics (except for the root length) while the concentrations of proline and leaves total soluble sugar with increasing levels of drought stress were significantly increased compared to control. The results showed that the accumulation of proline and soluble sugars were good indicators of drought tolerance. With increasing levels of drought stress, amount of leaves proline increased more than 5 times compared to control. Also, by increasing the drought stress severity from control (no stress) to 75% depletion of available water treatment, the content of leaves soluble sugars increased 2 times compared to control. Generally, results showed that this plant is tolerant to the drought stress by osmotic adjustment and this mechanism resulted in plant survival under drought stress conditions.

Keywords

Allen, S.E., 1989. Analysis of vegetation and other organic materials. In: S.E. Allen (ed.), Chemical Analysis of Ecological Materials, third edition. p. 46–60. Blackwell Scientific Publications, Oxford, England.
Bates, L.S., Waldren, R.P., Treare, I.D., 1973. Rapid determination of free proline for water stress studies. Plant and Soil. 39, 205-207.
Chaves, M.M., Maroco, J.P., Pereira J.S., 2003. Understanding plant response to drought: from genes to the whole plant. Functinal  Plant Biology. 30, 239-264.
Hameed, M., Ashraf. M., 2008. Physiological and biochemical adaptations of Cynodon dactylon (L.) Pers. from the salt range (Pakistan) to salinity stress. Flora. 203, 683-694.
Hanson, A.D., Hitz, W.D., 1982. Metabolic responses of plant water deficit. Annual Review of Plant Physiology. 33, 163-203
Hashemi dezfuli, A., Kochaki, E., Banaian, M., 1995. Increasing Crop Yield. Iranian Academic Center for Education, Culture and Research (ACECR), Mashhad Publication. 360p. [In Persian].    
Jampeetong, A. Brix. H., 2009. Effect of NaCl salinity on growth, morphology, photosynthesis and proline accumulation of Salvia natans. Aquatic Botany. 91, 181-186.
Manivannan, P., Abdul Jaleel, C., Sankar, B., Kishorekumar, A., Somasundaram, R., Lakshmanan, G.M.A., Panneerselvam, R., 2007. Growth, biochemical modifications and proline metabolism in Helianthus annuus L. as induced by drought stress. Colloids and Surfaces, B: Biointerfaces. 59, 141-149.
Sadizadeh, M., Abbassi, F., Baghizadeh, A., Yazdanpanah, A., 2009. The effects of salicylic acid and ascorbic acid on some of resistance mechanisms to drought stress in Echium amoenum. American-Eurasian Journal of Agricultural and Environmental Science. 6(3), 262-267.  
Zhao, K.F., Harris, P.J., 1992. The effects of iso-osmotic salt and water stresses on the growth of halophyte and non-halophyte. Journal  of . Plant Physiology. 139, 761-763.