Document Type : Original Article

Authors

• Hamed Derogar ¹
• Baratali Fakheri ²
• Nafiseh Mehdinezhad ³
• Rahmat Mohammadi ⁴

¹ Graduated M.Sc. Student, Department of Plant Breeding and Biotechnology, Collage of Agriculture, University of Zabol, Zabol, Iran.

² Professor, Department of Plant Breeding and biotechnology, Collage of Agriculture, University of Zabol. Zabol, Iran

³ Assistant Professor, Department of Plant Breeding and biotechnology, Collage of Agriculture, University of Zabol, Zabol, Iran

⁴ Assistant Professor, Dryland Agricultural Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Maragheh, Iran

Abstract

Introduction
Wheat is the most widely grown crop in the world, which has the highest crop area.. Ageilops tauschii, Aegilops speltoides, Triticum urartu, are diploids In terms of ploidy level, diploid species (2n=2x=14), from wild relatives of wheat and, respectively, has a DD genome and a genome D agent in modern bread wheat, Includes BB genome and AA genome. Drought stress is an abiotic stress that is considered as one of the important factors in decreasing plant growth and crop production in most parts of the world, especially in Iran, and it leads to oxidative stress, Plants have different potent in drought stress in all aspects of development, including physiological, biochemical, morphological and anatomical, so plants use different strategies and protection systems against stress. The most important strategies are the change in antioxidant enzymes activity and increase or decrease in protein-induced proteolysis and changes in the chlorophyll and carotenoid levels. The aim of this study is to investigate the effect of different drought levels stress on some physiological traits in diploid (wild cultivars), tetraploid (durum wheat) and hexaploid (bread wheat) species.

Materials and methods
In order to investigate the effect of different drought stress levels on some biochemiccal characteristics, a research project was conducted in 2016-2017 in the greenhouse of Zabol Agricultural Faculty as factorial experiment in a completely randomized design with three replices. The first factor was wheat species and cultivars (Tauschii, Speltoides, Urartu, Shabrang, Behrang, Sistan, Argh) and the second factor was drought stress (90, 70, 50 and 30 percent of field capacity). The traits studied in this study were antioxidant activity (including superoxide dismutase, catalase and ascorbate peroxidase), protein, chlorophyll a, chlorophyll b and malondialdehyde activity. Data analysis was performed using SAS software version 9/9, the mean of data was analyzed by Tukey’s test (p≤0.01) and graph charts were drawn by Excel software.

Results and discussion
The results showed that drought stress had significant effect on the concentrations of protein, chlorophyll a and b, carotenoid, catalase, superoxide dismutase, ascorbate peroxidase and malondialdehyde. That's while with increasing the stress, the amount of ascorbate peroxidase varied, so that initially increased and then decreased. This represents the activation of antioxidant system in different wheat species to increase drought tolerance. With increasing drought stress up to 50 percent of field capacity, protein, chlorophyll and carotenoid amounts increased, but with the higher stress these amounts decreased. So it can be stated that to reduce the damage caused by water shortages in the higher stress, the plant has resisted to drought by increasing proteolysis and decreasing protein amount.

Conclusion
Due to the fact that plants tolerate some degrees of stress, the studied wheat cultivars were able to tolerate 50 percent of field capacity, and with increasing the stress subsequently to higher levels, they showed a decrease in activity and some biochemical traits. Among all the cultivars, Shabrang showed the highest values. Therefore, in this experiment as it was shown by other researches, the superiority of this cultivar has been determined. Also, the lowest amount was related to Sistan cultivar, which received the lowest values, However, during the process of applying stress, the savage wheat cultivars were superior for these traits. Therefore, further studies are necessary on the wild wheat varieties.

Acknowledgements
We are thankful to Dr. Kamal ghasemibezdi and members of the Faculty of Agriculture Research Laboratory.

Keywords

• Antioxidant enzymes
• chlorophyll
• Malondialdehyde
• Protein
• Wheat species