Environmental Stresses in Crop Sciences

Current Issue

By Issue

By Author

By Subject

Author Index

Keyword Index

About Journal

Aims and Scope

Editorial Board

Publication Ethics

Indexing and Abstracting

FAQ

Peer Review Process

Journal Forms

Paper Preparation Guide

Effect of drought stress on NAC gene expression in some bread wheat cultivars of Sistan region

Document Type : Original Article

Authors

1 M.Sc. Student, Department of Plant Breeding and Biotechnology, University of Zabol, Zabol, Iran.

2 Associate Professor of Department of plant Breeding and Biotechnology, University of Zabol, Zabol, Iran.

3 Assistant of Agriculture and Biotechnology Research Institute, university of Zabol, Zabol, Iran.

Abstract

Introduction
Plant growth is severely influenced by environmental stresses such as drought, high salinity and extreme temperatures. Environmental stresses trigger a wide range of plant responses, like change in the gene expression to variability in cellular metabolism and growth. In the past years investigation on abiotic stresses, especially drought stress, has focused on plant resistance genes and their mechanism of function. This research revealed that a majority of the genes that have important roles in the biotic and abiotic stresses resistance encode transcription factors. Hence, the identification of genes especially regulatory ones whose expression enables plants to adapt to or to tolerate these abiotic stresses, is very essential. The NAC gene family is one of the types of genes related to transcription factors that is expressed in different tissues and growth stages. In a number of transcription factors effective in aging of the leaf has been identified. The transcription factors of this family are more associated with aging related genes and growth and development processes in response to stresses in many crops. The objective of this study was to investigate the tolerate of five wheat cultivars (Hamoon, Hirmand, Kavir, Boulani and Boulani Cross) under drought stress conditions (5, 10, 15, 20 and 25% Field Capacity) based on ratio of NAC gene relative expression and osmotic regulators modifications.
Materials and methods
A factorial experiment was carried out using a randomized complete block design (RCBD) with three replications during the 2017–2018 at Biotechnology Research Institute of Zabol University, Zabol, Sistan and Baluchestan Province, Iran. The experimental treatments included bread wheat cultivars (Hamoon, Hirmand, Kavir, Boulani and Boulani Cross) and drought stress levels (5, 10, 15, 20 and 25% Field capacity). The bread wheat cultivars were planted in pot. Drought stress was applied at seedling stage (four to five leaves). Physiological traits (such as Polyphenol oxidase and Proline) and the level of expression of the TaNAC67 gene were estimated in this experiment. Data analysis was performed using Ratio =2 -ΔΔCT and SAS software version 9.1.

Results
The results of analysis of variance (ANOVA) showed that, the effect of cultivar, drought stress and interaction of drought stress × cultivar on the relative expression of NAC gene and osmotic regulators at drought stress levels (20, 15, and 5% Field capacity) compared to normal levels (25% Field capacity) was significant. Based on the obtained results, increasing of drought stress severity increased studied traits. The results of simple effects of drought stress levels showed that increasing the levels of drought stress, the traits were increased, so that The highest TaNAC gene expression, Polyphenol oxidase and Proline of leaf was obtained in plants treated with drought stress 5% crop capacity.
The results of this experiment showed that increasing the levels of drought stress with 20, 15, 10, 5% of the field capacity compared to the normal level of 25% of field capacity, among the five cultivars of bread wheat, the relative expression of NAC gene increased. In addition to increasing the relative expression of NAC gene in the seedling stage, Polyphenol Oxidase and proline accumulation increased in Hirmand cultivar and then Boulani Cross cultivar.
Conclusion
According to the findings of this experiment through the five Sistan region bread wheat cultivars, the Hirmand and then Boulani Cross cultivars indicated better response than other genotypes to drought stress. As a result, due to the coherence of the molecular results and studied traits, Hirmand cultivar had the best response under drought stress conditions. Performing more complementary experiments is mandatory to confirm the obtained results from Hirmand cultivar in this work.

Keywords

References
Abogadallah, G.M., 2010. Antioxdative defense under salt stress. Plant Signaling and Behavior 5(4), 369-374. ]In Persian with English summary].
Ackerson, R.C., 1980. Stomatal response of cotton to water stress and abscisic acid as affected by water stress history. Plant Physiology. 65, 455-459.
Agarwal, S., Pandey, V., 2004. Antioxidant enzyme responses to NaCl stress in Cassia angustifolia. Biologia Plantarum. 48, 555-560
Ajith, T.A., Janardhanan, K.K., 2007. Indian medicinal mushrooms as a source of antioxidant and antitumor agents. Journal of Clinical Biochemistry and Nutrition. 40(3), 157-162. ‏
Allagulova, C.R., Gimalov, F.R., Shakirova, F.M., Vakhitov, V.A., 2003. The plant dehydrins: structure and putative functions. Biochemistry. 68(9), 945-951.
Amiri Deh Ahmadi, S.R., Ganjeali Mohamadi, E., 2010. Effects of drought stress on morphological characteristics and yield components in different phenological stages of chickpea (Cicer arietinum L) greenhouse conditions. Journal of Agricultural Research. 8(1), 157-166. ]In Persian with English summary].
Ashraf, M., 2009. Biotechnological approach of improving plant salt tolerance using antioxidants as markers. Biotechnology advances. 27(1), 84-93.
Ashraf, M., 2010. Inducing drought tolerance in plants: recent advances. Biotechnology Advances. 28(1), 169-183.
Bajji, M., Lutts, S., Kinet, J.M., 2001. Water deficit effects on solute contribution to osmotic adjustment as a function of leaf ageing in three durum wheat (Triticum durum Desf) cultivars performing differently in arid conditions. Plant Science. 160, 669-681.
Bates, S., Waldern, R.P., Teare, E.D., 1973. Rapide determination of free proline for water stress studies. Plant and Soli. 39, 205-207.
Cai, H., Tian, S., Dong, H., Guo, C., 2015. Pleiotropic effects of TaMYB3R1 on plant developmentand response to osmotic stress in transgenic Arabidopsis. Journal of Gene. 558, 227-234.
Ceccarelli, S., 2010. Drought and drought resistance. Encyclopedia of Biotechnology in Agriculture and Food. 1, 205-207. ‏
Cook, R.J., Veseth, R.J., 1991. Wheat health management (p. 152). St. Paul, MN: APS Press.
Dai, H.P., Shan, C.J., Wei, A.Z., Yang, T., Sa, W.Q., Feng, B.L., 2012. Leaf senescence and photosynthesis in foxtail millet (Setaria Italica L. P. Beauv) varieties exposed to drought conditions. Australian Journal of Crop Science. 6(2), 232-237.
Dat, J., Vandenabeele, S., Vranová, E., Van Montagu, M., Inzé, D., Van Breusegem, F., 2000. Dual action of the active oxygen species during plant stress responses. Cellular and Molecular Life Sciences. 57(5), 779-795.
de Azevedo Neto, A.D., Prisco, J.T., Enéas-Filho, J., de Abreu, C.E.B., Gomes-Filho, E., 2006. Effect of salt stress on antioxidative enzymes and lipid peroxidation in leaves and roots of salt-tolerant and salt-sensitive maize genotypes. Environmental and Experimental Botany. 56, 87-94.
Delauney, A.J., Verma, D.P.S., 1993. Proline biosynthesis and osmoregulation in plants. Plant Journal. 4, 215-223.
Du, J. B., Yuan, S., Chen, Y. E., Sun, X., Zhang, Z. W., Xu, F., Lin, H. H., 2011. Comparative expression analysis of dehydrins between two barley varieties, wild barley and Tibetan hulless barley associated with different stress resistance. Acta Physiologiae Plantarum. 33(2), 567-574. ‏
Dubos, C., Stracke, R., Grotewold, E., Weisshaar, B., Martin, C., Lepiniec, L., 2010. MYB transcription factors in Arabidopsis. Trends in plant science. 15(10), 573-581.
Esfandiari, E, Javadi, A, Shokrpour, M., Shekari, F., 2011. The effect of salt stress on the antioxidant defense mechanisms on wheat seedling. Fresenius Environmental Bulletin. 20(8), 2021-2036.
FAO, F., 2015. Agriculture Organzation of the United Nations, FAO, Food and Agriculture Organization of the United Nations. Greenhouse gas emissions from the dairy sector. A life cycle assessment. FAO, Rome, Italy.
Farshadfar, E., Farshadfar, M., Sutka, L., 2000. Combining ability analysis of drought tolerance in wheat over differrnt water regimes. Acta Agronomica Hungarica. 48(4), 353-361.
Ghani, F., Ghasemi PirBaloti, A., Hamedi, B., Malekpour, F., 2011. Effect of different levels of water and nitrogen on morphological, physiological and physiological traits of Matricaria aurea L. herbal remedies. 2(2), 101-111. ]In Persian with English summary].
Gharbi, A., Rashid I.N., Tarynzhad. A.S, Chlbyyany. Q., 2013. Salinity and drought tolerance of durum wheat lines under greenhouse conditions. Journal of Crop Ecophysiology. 4(28), 393-410. ]In Persian with English summary].
Ghasemi Pirbalouti, A., Samani, M. R., Hashemi, M., Zeinali, H., 2013. Salicylic acid affects growth, essential oil and chemical compositions of thyme (Thymus daenensis Celak) under reduced irrigation. Plant Growth Regulation. 72(3), 289-301.
Gill, S.S., Tuteja, N., 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry. 48(12), 909-930.
Guo, X.Y., Zhang, X.S., Huang, Z.Y., 2010. Drought tolerance in three hybrid poplar clones submitted to different watering regimes. Journal of Plant Ecology. 3(2), 79-87. ‏
Hartung, W., 1983: The site of action of abscisic acid at the guard cell plasmalemma of Valerianella locusta. Plant, Cell and Environment. 6, 427-428
Hernández, J.A., Ferrer, M.A., Jiménez, A., Barceló, A.R., Sevilla, F., 2001. Antioxidant Systems and O2/H2O2 production in the apoplast of pea leaves. Its relation with salt-induced necrotic lesions in minor veins.  Journal of Plant Physiology. 127(3), 817-831.
Hissao, T., 1973. Plant responses to water stress. Annual Review of Plant Biology. 24, 519-570.
Janovitz-Klapp, A.H., Richard, F.C., Goupyand, P.M.J., Nicolas, J., 1990. Inhibition studies on apple polyphenol oxadase. Journal of Agricultural Food Chemistry. 38, 926-931.
Javadi, S. M., Shabr, Z. S., Pour Abed, A., Ghadiri, Sh., 2016. Reconstruction and Analysis of Drought Tolerance Gene Networks in Hordeum Vulgare Leaf. The First International Conference and the 9th National Biotechnology Conference of the Islamic Republic of Iran. 3-5 May. International Center of Shahid Beheshti University. ]In Persian with English summary].
Lata, C., Jha, S., Dixit, V., Sreenivasulu, N., Prasad, M., 2011. Differential antioxidative responses to dehydration-induced oxidative stress in core set of foxtail millet cultivars. Protoplasma. 248(4), 817-828.
Livak, K.J., Schmittgen T.D., 2001. Analysis of relative gene expression data using real time quantitative PCR and the 2-∆∆CT Method. Methods. 25(4), 402–408.
Mardeh, A. S. S., Ahmadi, A., Poustini, K., Mohammadi, V., 2006. Evaluation of drought resistance indices under various environmental conditions. Field Crops Research. 98(2): 222-229.
Metwali, M.R., Ehab-Manal, H., Tarek, E., Bayoumi, Y., 2011. Agronomical traits and biochemical genetic markers associated with salt tolerance in wheat cultivars (TriticumaestivumL). Australian Journal of Basic and Applied Sciences. 5(5), 174-183.
Mieeler, R., 2002. Oxidative stress, antioxidant and stress tolerance. Trends in Plant Science. 7(9), 405-415.
Mittler, R., 2002. Oxidative stress, antioxidants and stress tolerance. Trends Plant Science, 7, 405–410.
Nickavar, B., Kamalinejad, M., Hajyahya, M., Shafagh, B., 2006. Comparison of the free radical scavenging activity of six Iranian Achillea species. Pharmaceutical Bioligy. 44, 208-212.
Oberts, J., Tucker, G.T., 1998. Plant Hormone. Humana press.
Ornish, K., Zeevaart, J.A.D., 1985b: Abscisic acid accumulation by roots of Xanthium strumarium L. and (Lycopersicon esculentum Mill.) In relation to water. Plant Physiology. 79: 653-658.
Ozturk, Z.N., Talame, V., Deyholos, M., Michalowski, C.B., Galbraith, D.W., Gozukirmizi, N., Tuberosa, R., Bohnert, H. N., 2002. Monitoring large-scale changes in transcript abundance in drought-and salt-stressed barley. Plant Molecular Biology. 48(5), 551-573.
Peng, H., Cheng, H.Y., Chen, C., Yu, W., Yang, N., Gao, W., Ma, H., 2009. A NAC transcription factor gene of Chickpea (Cicer arietinum L), CarNAC3, is involved in drought stress response and various developmental processes. Journal of plant Physiology. 166(17), 1934-1945. ‏
Powell, L. M., Pfdifer, P., 1956. The effect of controlled limitroled moisture on seedinas of cheyenne winter wheat selections. Agronomy Journal. 48, 555-557.
Rahaei, M., Naghavi, M., Alizadeh, H., Malboobi, M.A., Abdemishani, S., Cyrus, Shank, P., Jupp, G., 2009. Investigation of MYB gene expression pattern in wheat (Triticum aestivum L.) under two short-term salinity and cold stresses using qRT-PCR. Iranian Journal of Crop Sciences. 41(3), 436-433. ]In Persian with English summary].
Ranney, T.G., Bassuk, N.L., Whitlow, T.H., 1991. Osmotic adjustment and solute constituents in leaves and roots of water-stressed cherry (Prunus) trees. Journal of the American Society for Horticultural Science. 116(4), 684-688.
Rascio, A., Russo, M., Platani, C., Difonzo, N. 1998. Drought intensity effects on genotypic differences in tissue affinity for strongly bound water. Plant Science. 132, 121-126.
Reddy, A.R., Chaitanya, K. V., Vivekanandan, M., 2004. Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants. Journal of Plant Physiology. 161(11), 1189-1202. ‏
Riechmann, L., Ratcliffe, O.J., 2000. Agenomic perspective on plant transcription factors. Journal of Current Opinion in Plant Biology. 3, 423-434.
Saad, A.S.I., Li, X., Li, H.P., Huang, T., Gao, C.S., Guo, M.W., Liao, Y.C., 2013. Arice stress-responsive NAC gene enhances tolerance of transgenic wheat to drought and salt stresses. Plant Science. 203, 33-40. ‏
Sahrawat, A.K., Becker, D., Lutticke, S., Lorz, H., 2003. Genetic improvement of wheat via alien gene transfer, an assessment. Plant Science. 165(5), 1147-1168.
Sardmadnia, H., Kochaki, A., 1994. The Importance of Environmental Stresses in Agriculture. First Iranian Plant Breeding and Congress. Agricultural University of Karaj, Tehran Agricultural University, pp. 169-157. ]In Persian with English summary].
Shabestari, M., 1990. Crop Physiology. Academic Publication Center, Tehran, 214P. ]In Persian with English summary].
Solomon, A., Beer, S., 1994. Effect of NaCl on the carboxylating activity of rubisco and absence of proline related compatible solutes Plant. Physiology. 108, 1387-1394.
Tarahomi, P., Lahooti, D., Abbasi, P., 2010. Effects of drought stress on soluble sugars, chlorophyll and potassium of Salvia leriifolia Benth. Journal of Biological Sciences. 3(2), 1-7. ]In Persian with English summary].
Tardieu, F., 2012. Any trait or trait-related allele can confer drought tolerance: just design the right drought Scenario. Journal of Experimental Botany. 63(1), 25-31.
Turkan, I., Bor, M., Ozdemir, F., Koca, H., 2005. Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Science. 168(1), 223-231. ‏
Turner, N.C., 1986. Adaptation to water deficits: A changing perspective. Austral Journal Plant Physiology. 13, 175-190.
Uauy, C., Distelfeld, A., Fahima, T., Blechl, A., Dubcovsky, J., 2006. A NAC gene regulating senescence improves grain protein, zinc, and iron content in wheat. Science. 314, 1298-1301.
Uno, Y., Furihata, T., Abe, H., Yoshida, R., Shinozaki, K., Yamaguchi-Shinozaki, K., 2000. Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions. Proceedings of the National Academy of Sciences. 97(21), 11632-11637. ‏
Xoconostle-Cázares, B., Ramirez-Ortega, F. A., Flores-Elenes, L., Ruiz-Medrano, R., 2010. Drought tolerance in crop plants. American Journal of Plant Physiology. 5(5), 1-16.
Yamaguchi-Shinozaki, K., Koizumi, M., Urao, S., Shinozaki, K., 1992. Molecular cloning and characterization of 9 cDNAs for genes that are responsive to desiccation in Arabidopsis thaliana: sequence analysis of one cDNA clone that encodes a putative transmembrane channel protein. Plant and Cell Physiology. 33(3), 217-224.
Yar Hussain, M., Heydariyan, Z., Neazaei, A., Ramezani, I., 2011. Examined the expression levels of two genes encoding the transcription factors related -MYB family in two varieties of wheat under salt stress. National Biotechnology Congress of Islamic Republic of Iran. ]In Persian].
Zhang, J., Kirkham, M.B., 1995. Water relations of water-stressed, split-root C4 (Sorghum bicolor) and C3 (Helianthus annuus) plants. American Journal of Botany. 1220-1229. ‏
Zhao, X., Kang, M., Ren, M., Chen, S. 1995. Analysis on combining ability of yield characters in common wheat. Acta Agriculturae Boreali-Sinica. 10: 38-41.
Zheng, X., Chen, B., Lu, G., Han, B., 2009. Overexpression of a NAC transcription factor enhances rice drought and salt tolerance. Biochemical and Biophysical Research Communications. 379(4), 985-989. ‏
Zhu, J.K., 2003. Regulation of ion homeostasis under salt stress. Current Opinion in Plant Biology. 6(5), 441-445.
Environmental Stresses in Crop Sciences
Volume 12, Issue 3
Open Access Policy
October 2019
Pages 649-662
Files
History
  • Receive Date: 21 May 2018
  • Revise Date: 20 August 2018
  • Accept Date: 11 September 2018
Share
How to cite
Statistics