Document Type : Original Article

Authors

1 Assistant Professor of Gonbad Kavous University

2 Associate Professor of Shahrood Industrial University

Abstract

Introduction
Raey et al. (2008) reported that irrigation was affected on traits such as number of pods per plant, 1000-seed weight, seed yield and harvest index. Nayer et al. (2006) believed that flowering and pod setting are sensitive stages to water stress in chickpea (Cicer arietinum L.). water stress in pollination stage reduced severity yield and phosphorous concentration of chickpea seed relative to vegetative stage (Gunes et al., 2006). Yazdani et al. (2009) studied effect of phosphate solubilisation microorganisms and plant growth promoting rhizobacteria on corn plant and reported that application of PSM and PGPR were increased yield of corn and phosphorous needs to 50%. The aim of this study was to determine the effect of inoculation with various mycorrhiza fungi and irrigation management on quantity and quality characteristics of chickpea (Cicer arietinum L.).
  Matherials and Methods
In order to study the response of chickpea inoculated with different mycorrhizal fungi under different irrigation management, a split plot experiment based on Randomized Complete Block Design carried out with four replications on farm research of Industrial University of Shahroud in Bastam (36° 30′ N, 55°00′ E, and 1380 m above sea level) during 2011. Four levels of irrigation management (as main plots) was complete irrigation, non irrigation in flowering stage, non irrigation in pod setting stage and non irrigation in seed filling stage. Three levels of inoculation (as sub plots) was inoculation with Glumos mosseae, inoculation with G. intraradices and non inoculation. Planting of the seeds was done during the 2nd week of April 2011. Sowing was performed manually. Row spacing was 30 and within rows was 10 cm (333000 plants/ha). The experimental plots were 6 rows. Weed control was performed manually. Seed yield was determined by harvesting of each crop from 4 middle rows after removing of two border rows and 0.5 meter from two sides of remains rows in 2nd week of July 2011. Data were analyzed using the SAS Ver. 9.1 computer software program and treatment mean differences were separated by the least significant difference (LSD) test at the 0.05 probability level.
Results and Discussion
The results showed that the effect of irrigation management on number of pods per plant, number of seeds per plant, 100-seed weight, Grain yield, harvest index, protein percentage, protein yield, phosphorus concentration and phosphorus yield were significant so that the most of the traits except protein was obtained from complete irrigation treatment. Differences between complete irrigation and non irrigation at seed filling stage in terms of number of pods per plant and number of seeds per plant was not significant. The effects of inoculated with mycorrhiza on all traits except number of pods per plant, number of seeds per plant and protein percentage of seed was significant and the most amounts of the traitss was obtained from inoculation with G. mosseae and the lowest belonged to non-inoculation treatment. Differences between two inoculated treatment of G. mosseae and G. intraradices in terms of 1000-seed weight was not significant. The interaction of water stress × inoculation with mycorrhiza was not significant. Seed and protein yield in complete irrigation was 2651 and 557.3 kg/ha and in non irrigation in flowering stage was 1472 and 337.3 kg/ha, respectively. Seed and protein yield in inoculation with Glumos mosseae was 2124 and 495.2 kg/ha and in non inoculation treatment was 1677 and 391.9 kg/ha, respectively.
Conclusion
Vegetative, Reproductive and quality traits was affected by irrigation management. water stress decreased all traits and earlier water stress had more effec on yield. Effect of inoculation was less than water stress and inoculation with G. mosseae had more effect than G. intraradices on traits.

Keywords

Abdoli, M., Saeidi, M., Jalali-Honarmand, S., Mansourifar, S., Ghobadi, M.E., 2016. Effects of photosynthetic source limitation and post-anthesis water deficiency on grain filling rate, photosynthesis and gas exchange in bread wheat cultivars. Environmental Stresses in Crop Sciences. 8(2), 131-147. [In Persian with English Summary].
Ahmad, F., Gaur, P., Croser, J. 2005. Chickpea (Cicer arietinum L.). In: Singh, R.J., Jauhar, P.P. (eds.), Genetic Resources, Chromosome Engineering and Crop Improvement, grain Legumes. Tailor and Francis Group. P. 229-267.
Ahmadi, K., Gholizadeh, H., Ebadzadeh, H., Hosseinpour, R., Hatami, F., Fazli, B., Kazemian, A.,Rafei, M. 2015. Agricultural statistics, Year 2003. Ministry of Agriculture. Vol. 1: Crops. 169 p.
Bolandnazar, S., Aliasgarzad, N., Neishabury, M.R., Chaparzadeh, N., 2007. Mycorrhizal colonization improves onion (Allium cepa L.) yield and water use efficiency under water deficiet condition. Scientia Horticulturae. 114(1), 11-15.
 Erman, M., Demir, S., Ocak, E., Tufenkci, S., Oguz Ahmet Akkopru, F., 2011. Effects of rhizobium, arbuscular mycorrhiza and whey applications on some properties in chickpea (Cicer arietinum L.) under irrigated and rainfed conditions. 1- Yield, yield components, nodulation and AMF colonization. Field Crops Research. 122(1), 14–24.
Grant, C., Bittman, S., Montreal, M., Plenehette, C., Morel, C., 2005. Soil and fertilizer phosphorus: Effects on plant P supply and mycorrhiza development. Canadian Journal of Plant Science. 85, 3-14.
Gunes, A., Cicek, N., Inal, A., Alpaslan, M., Erasalan, F., Guneri, F., Guzelordu, E., 2006. Genotypic response of chickpea cultivars to drought stress implemented at pre- and post- anthesis stages and its relations with nutrient uptake and efficiency. Plant, Soil and Environment. 52(8), 368-376.
Hu, Y., Burucs, Z., Schmdihalter, U., 2008. Effect of foliar fertilization application on the growth and mineral nutrient content of maize seedlings under drought and salinity. Soil Science and Plant Nutrition. 54, 133-141.
Jalilian, J., Modarres Sanavy, S.A.M., Sabaghpour, S.H. 2005. Effect of plant density and supplemental irrigation on yield, yield components and protein content of four chickpea (Cicer arietinum L.) cultivars under dry land condition. Journal of Agricultural Science and Natural Resources. 12(5), 55-64.
Kaschuk, G., Leffelaar, P.A., Giller, K.E., Alberton, O., Hungria, M., Kuyper, T.W., 2010. Responses of legumes to rhizobia and arbuscular mycorrhizal fungi: A meta-analysis of potential photosynthate limitation of symbioses. Soil Biology and Biochemistry. 42, 125-127.
Khan, I.A., Ayub, N., Mirza, S.N., Nizami, S.M., Azam, M., 2008. Yield and water use efficiency of Cenchrus ciliaris as influenced by vesicular arbuscular mycorrhizae. Pakistan Journal of Botany. 40(2), 931-937.
Mohammadi, Gh., Ghasemi Golezani, K., Javanshir, A., Moghaddam, M., 2006. The influence of water limitation on the yield of three chickpea cultivars. Journal of Water and Soil Science. 10(2), 109- 120. [In Persian with English Summary].
Nayyar, H., Sigh, S., Kaur, S., Kumar, S., upadhyaya, H.D., 2006. Differential sensitivity of macrocarpa and microcarpa types of chickpea to water stress: Association of contracting stress response with oxidative injury. Integrative Plant Biology. 48, 1318- 1329.
Raey, Y., Demaghsi, N., Seied Sharifi, R., 2008. Effect of different levels of irrigation and plantdensity on grain yield and its components in chickpea (Cicer arietinum L.) Deci type cv. Kaka. Iranian Journal of Crop Sciences. 9 (4), 371-381. [In Persian with English Summary].
Rezvani Moghaddam, P., Sadeghi Samarjan, R., 2008. Effect of sowing dates and different irrigation regimes onmorphological characteristics and grain yield of chickpea (Cicer arietinum L.) (cultivar 3279 ILC). Iranian Journal of Field Crops Research. 6(2), 315-325. [In Persian with English Summary].
Soil and Water Research Institute. 1996. Methods of Plant Analysis. Vol. 1, No. 982. [In Persian].
Turner, N., Xiangwen, F., Fengmin, L., Kadambot, H.M., 2009. Flower numbers, pod production, pollen viability, and pistil function are reduced and flower and pod abortion increased in chickpea (Cicer arietinum) under terminal drought. Journal of Experimental Botany. 61(2), 335- 345.
Yazdani, M., Bahmanyar, M.A., Pirdashti, H., Esmaili, M.A., 2009. Effect of phosphate solubilisation microorganisms (PSM) and plant growth promoting rhizobacteria (PGPR) on yield and yield components of corn (Zea mays L.). International Journal of Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering. 3(1), 50-52.