نوع مقاله : مقاله پژوهشی
نویسندگان
گروه علوم و زیست‌فناوری گیاهی، دانشکده علوم زیستی و بیوتکنولوژی، دانشگاه شهید بهشتی، تهران
چکیده
سیب‌زمینی یکی از گیاهان مهم زراعی در جهان است. اما سیب‌زمینی تحت تنش‌های غیرزیستی مخصوصا خشکی و سرما قرار می‌گیرد. تنش‌های سرما و خشکی از جمله مهم‌ترین تنش‌های غیرزیستی در سراسر جهان هستند که منجر به کاهش عملکرد گیاهان زراعی می‌شوند. فاکتورهای اتصال C-تکرار (CBF) همچنین به عنوان اعضای خانواده پروتئین متصل به عناصر پاسخگو به کم‌آبی (DREB1) درنظر گرفته‌می-شوند. اما در سیب‌زمینی، سازوکار پایه‌ای از تحمل به تنش مبهم باقی مانده است. آزمایشی در قالب طرح بلوک کامل تصادفی با سه تکرار تحت تنش خشکی و سرما در گلخانه کرج انجام شد. در این مطالعه، بررسی بیان ژنCBF1 از برگ و ریشه تحت تنش سرما و خشکی در ژنوتیپ 8707.29 سیب‌زمینی انجام شد. متوسط میزان بیان ژن CBF1 ممکن همبستگی بالایی با کسب تحمل به استرس سرما و خشکی در ریشه داشت. درحالی‌که بیان این ژن در برگ کاهش داشته است. تحت تنش خشکی بیان ژن CBF1 در ریشه افزایش داشته است اما در برگ میزان بیان CBF1 تحت تنش کاهش بیان را نشان داد. پاسخ‌هایی که بین بخش‌های مختلف متفاوت هستند ممکن است بازتاب‌کننده مکانیسم‌های تحمل در تنش مخصوص بافت باشند که نشان‌دهنده اثر بافت اکولوژیکی در گسترش تحمل به استرس در سیب‌زمینی است. در این مطالعه بررسی جایگاه اتصالی فاکتورهای رونویسی، ژن‌های هم بیان و انتولوژی ژن‌ها در CBF1 در سیب-زمینی انجام شد. با توجه به آنالیز شبکه هم‌بیان، این ژن بیشتر در تحمل به سرما و خشکی دخالت داشت که سایر ژن‌های هم‌بیان احتمالا در بالادست مسیر بیوسنتز CBF1 عمل می‌کنند. نتایج مطالعه حاضر نشان داد که بیان ژن CBF1 در ریشه تحت تنش سرما و خشکی بیشتر از برگ بود. در مجموع ژن CBF1 می تواند در القای تحمل به تنش سرما و خشکی نقش داشته باشد و برای دست‌ورزی ژنتیکی و اصلاح سیب زمینی تحت تنش‌های فوق‌الذکر مورد استفاده قرار گیرد.
کلیدواژه‌ها
موضوعات
Abdullah, S.N.A., Azzeme, A.M., Yousefi, K., 2022. Fine-tuning cold stress response through regulated cellular abundance and mechanistic actions of transcription factors. Frontiers in Plant Science. 13, 850216-850216. https://doi.org/10.3389/fpls.2022.850216
An, D., Yang, J., Zhang, P., 2012. Transcriptome profiling of low temperature-treated cassava apical shoots showed dynamic responses of tropical plant to cold stress. BMC Genomics. 13, 1-25. https://doi.org/10.1186/1471-2164-13-64
Artlip, T.S., Wisniewski, M.E., Bassett, C.L. and Norelli, J.L., 2013. CBF gene expression in peach leaf and bark tissues is gated by a circadian clock. Tree Physiology. 33, 866-877. https://doi.org/10.1186/1471-2164-13-64
Benedict, C., Skinner, J.S., Meng, R., Chang, Y., Bhalerao, R., Huner, N.P., Finn, C.E., Chen, T.H., Hurry, V., 2006. The CBF1‐dependent low temperature signalling pathway, regulon and increase in freeze tolerance are conserved in Populus spp. Plant, Cell and Environment. 29, 1259-1272. https://doi.org/10.1111/j.1365-3040.2006.01505.x
Chen, J.Q., Meng, X.P., Zhang, Y., Xia, M., Wang, X.P., 2008. Over-expression of OsDREB genes lead to enhanced drought tolerance in rice. Biotechnology Letters. 30, 2191-2198. https://doi.org/10.1007/s10529-008-9811-5
Dong, C., Zhang, Z., Ren, J., Qin, Y., Huang, J., Wang, Y., Cai, B., Wang, B., Tao, J., 2013. Stress-responsive gene ICE1 from Vitis amurensis increases cold tolerance in tobacco. Plant Physiology and Biochemistry. 71, 212-217. https://doi.org/10.1016/j.plaphy.2013.07.012
Feng, H.L., Ma, N.N., Meng, X., Zhang, S., Wang, J.R., Chai, S., Meng, Q.W., 2013. A novel tomato MYC-type ICE1-like transcription factor, SlICE1a, confers cold, osmotic and salt tolerance in transgenic tobacco. Plant Physiology and Biochemistry. 73, 309-320. https://doi.org/10.1016/j.plaphy.2013.09.014
González-Martínez, S.C., Wheeler, N.C., Ersoz, E., Nelson, C.D., Neale, D.B., 2007. Association genetics in Pinus taeda L.I. wood property traits. Genetics. 175, 399-409. https://doi.org/10.1534/genetics.106.061127
Guo, J., Ren, Y., Tang, Z., Shi, W., Zhou, M., 2019. Characterization and expression profiling of the ICE-CBF-COR genes in wheat. The Journal of Life and Environment. 7, 2-19. https://doi.org/10.7717/peerj.8190
Hajibarat, Z., Saidi, A., Mosuapour, G.A., Ghaffari, M.R., Zienalabedini, M., 2020. Evaluation of drought tolerance of potato (Solanum Tuberosum L.) under water deficit. 12, 102-112. https://doi.org/10.52547/jcb.12.35.102
Hajibarat, Z., Saidi, A., Zeinalabedini, M., Gorji, A.M., Ghaffari, M.R., Shariati, V., Ahmadvand, R., 2022a. Genome-wide identification of StU-box gene family and assessment of their expression in developmental stages of Solanum tuberosum. Journal of Genetic Engineering and Biotechnology. 20, 1-21. https://doi.org/10.1186/s43141-022-00306-7
Hajibarat, Z., Saidi, A., Hajibarat, Z. 2022b. Genome wide identification of 14–3-3 gene family and characterization of their expression in developmental stages of Solanum tuberosum under multiple biotic and abiotic stress conditions. Functional and Integrative Genomics. 22, 1377-1390. https://doi.org/10.1007/s10142-022-00895-z
Heidari, P., 2019. Comparative analysis of C-repeat binding factors (CBFs) in tomato and Arabidopsis. Brazilian Archives of Biology and Technology. 62, 1-9. https://doi.org/10.1590/1678-4324-2019180715
Jeknić, Z., Pillman, K.A., Dhillon, T., Skinner, J.S., Veisz, O., Cuesta-Marcos, A., Hayes, P.M., Jacobs, A.K., Chen, T.H., Stockinger, E.J., 2014. Hv-CBF2A overexpression in barley accelerates COR gene transcript accumulation and acquisition of freezing tolerance during cold acclimation. Plant Molecular Biology. 84, 67-82. https://doi.org/10.1007/s11103-013-0119-z
Jin, Y.N., Zhai, S., Wang, W., Ding, X., Guo, Z., Bai, L., Wang, S., 2018. Identification of genes from the ICE–CBF–COR pathway under cold stress in Aegilops–Triticum composite group and the evolution analysis with those from Triticeae. Physiology and Molecular Biology of Plants. 24, 211-229. https://doi.org/10.1007/s12298-017-0495-y
Livak, K.J., Schmittgen, T.D., 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods. 25, 402-408. https://doi.org/10.1006/meth.2001.1262
Maestrini, P., Cavallini, A., Rizzo, M., Giordani, T., Bernardi, R., Durante, M., Natali, L., 2009. Isolation and expression analysis of low temperature-induced genes in white poplar (Populus alba). Journal of Plant Physiology. 166, 1544-1556. https://doi.org/10.1016/j.jplph.2009.03.014
Mehrotra, S., Verma, S., Kumar, S., Kumari, S., Mishra, B.N., 2020. Transcriptional regulation and signalling of cold stress response in plants: an overview of current understanding. Environmental and Experimental Botany. 180, 104243. https://doi.org/10.1016/j.envexpbot.2020.104243
Mickelbart, M.V., Hasegawa, P.M., Bailey-Serres, J., 2015. Genetic mechanisms of abiotic stress tolerance that translate to crop yield stability. Nature Reviews Genetics. 16, 237-251. https://doi.org/10.1038/nrg3901
Morran, S., Eini, O., Pyvovarenko, T., Parent, B., Singh, R., Ismagul, A., Eliby, S., Shirley, N., Langridge, P., Lopato, S., 2011. Improvement of stress tolerance of wheat and barley by modulation of expression of DREB/CBF factors. Plant Biotechnology Journal. 9, 230-249. https://doi.org/10.1111/j.1467-7652.2010.00547.x
Nakashima, K., Ito, Y., Yamaguchi-Shinozaki, K., 2009. Transcriptional regulatory networks in response to abiotic stresses in Arabidopsis and grasses. Plant Physiology. 149, 88-95. https://doi.org/10.1104/pp.108.129791
Park, S., Lee, C.M., Doherty, C.J., Gilmour, S.J., Kim, Y., Thomashow, M.F., 2015. Regulation of the Arabidopsis CBF regulon by a complex low‐temperature regulatory network. The Plant Journal. 82, 193-207. https://doi.org/ 10.1111/tpj.12796
Pino, M.T., Skinner, J.S., Jeknić, Z., Hayes, P.M., Soeldner, A.H., Thomashow, M.F., Chen, T.H., 2008. Ectopic AtCBF1 over‐expression enhances freezing tolerance and induces cold acclimation‐associated physiological modifications in potato. Plant, Cell and Environment. 31, 393-406. https://doi.org/10.1111/j.1365-3040.2008.01776.x
Saidi, A., Hajibarat, Z., 2020. In-silico analysis of eukaryotic translation initiation factors (eIFs) in response to environmental stresses in rice (Oryza sativa). Biologia. 75, 1731-1738. https://doi.org/1024.2478/s11756-20-00467-1
Saidi, A., Hajibarat, Z., Ahmadikhah, A., 2021. Computational analysis of responsive transcription factors Involved in drought and salt stress in rice. Journal of Applied Biotechnology Reports. 8, 406-413. https://doi.org/10.30491/JABR.2020.243913.1272
Sakuma, Y., Liu, Q., Dubouzet, J.G., Abe, H., Shinozaki, K., Yamaguchi-Shinozaki, K., 2002. DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration-and cold-inducible gene expression. Biochemical and Biophysical Research Communications. 290, 998-1009. https://doi.org/10.1006/bbrc.2001.6299
Shi, Y., Huang, J., Sun, T., Wang, X., Zhu, C., Ai, Y., Gu, H., 2017. The precise regulation of different COR genes by individual CBF transcription factors in Arabidopsis thaliana. Journal of Integrative Plant Biology. 59, 118-133. https://doi.org/10.1111/jipb.12515
Shinozaki, K., Yamaguchi-Shinozaki, K., Seki, M., 2003. Regulatory network of gene expression in the drought and cold stress responses. Current Opinion in Plant Biology. 6, 410-417. https://doi.org/10.1016/s1369-5266(03)00092-x
Soltész, A., Smedley, M., Vashegyi, I., Galiba, G., Harwood, W., Vágújfalvi, A., 2013. Transgenic barley lines prove the involvement of TaCBF14 and TaCBF15 in the cold acclimation process and in frost tolerance. Journal of Experimental Botany. 64,1849-1862. https://doi.org/10.1093/jxb/ert050
Stockinger, E.J., Gilmour, S.J., Thomashow, M.F., 1997. Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit. Proceedings of the National Academy of Sciences. 94, 1035-1040. https://doi.org/10.1073/pnas.94.3.1035
Wang, G., Xu, X., Wang, H., Liu, Q., Yang, X., Liao, L., Cai, G., 2019. A tomato transcription factor, SlDREB3 enhances the tolerance to chilling in transgenic tomato. Plant Physiology and Biochemistry. 142, 254-262. https://doi.org/10.1016/j.plaphy.2019.07.017
Wang, L., Cao, H., Qian, W., Yao, L., Hao, X., Li, N., Yang, Y., Wang, X., 2017. Identification of a novel bZIP transcription factor in Camellia sinensis as a negative regulator of freezing tolerance in transgenic Arabidopsis. Annals of Botany. 119, 1195-1209. https://doi.org/10.1093/aob/mcx011
Welling, A., Palva, E.T., 2008. Involvement of CBF transcription factors in winter hardiness in birch. Plant Physiology. 147, 1199-1211. https://doi.org/10.1104/pp.108.117812
Wisniewski, M., Norelli, J., Bassett, C., Artlip, T., Macarisin, D., 2011. Ectopic expression of a novel peach (Prunus persica) CBF transcription factor in apple (Malus× domestica) results in short-day induced dormancy and increased cold hardiness. Planta. 233, 971-983. https://doi.org/10.1007/s00425-011-1358-3
Xiao, H., Siddiqua, M., Braybrook, S., Nassuth, A., 2006. Three grape CBF/DREB1 genes respond to low temperature, drought and abscisic acid. Plant, Cell and Environment. 29, 1410-1421. https://doi.org/10.1111/j.1365-3040.2006.01524.x
Xiao, H., Tattersall, E.A., Siddiqua, M.K., Cramer, G.R., Nassuth, A., 2008. CBF4 is a unique member of the CBF transcription factor family of Vitis vinifera and Vitis riparia. Plant, Cell and Environment. 31, 1-10. https://doi.org/10.1111/j.1365-3040.2007.01741.x
Yao, W., Wang, L., Wang, J., Ma, F., Yang, Y., Wang, C., Tong, W., Zhang, J., Xu, Y., Wang, X., Zhang, C., 2017. VpPUB24, a novel gene from Chinese grapevine, Vitis pseudoreticulata, targets VpICE1 to enhance cold tolerance. Journal of Experimental Botany. 68, 2933-2949. https://doi.org/10.1093/jxb/erx136
Zhao, C., Zhang, Z., Xie, S., Si, T., Li, Y., Zhu, J.K., 2016. Mutational evidence for the critical role of CBF transcription factors in cold acclimation in Arabidopsis. Plant Physiology. 171, 2744-2759. https://doi.org/10.1104/pp.16.00533
Zhao, J., Shi, M., Yu, J., Guo, C., 2022. SPL9 mediates freezing tolerance by directly regulating the expression of CBF2 in Arabidopsis thaliana. BMC Plant Biology. 22, 1-13. https://doi.org/10.1186/s12870-022-03445-8
Zhu, J., Dong, C.H., Zhu, J.K., 2007. Interplay between cold-responsive gene regulation, metabolism and RNA processing during plant cold acclimation. Current Opinion in Plant Biology. 10, 290-295. https://doi.org/10.1016/j.pbi.2007.04.010
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