Environmental Stresses in Crop Sciences

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The study of responses of garden thyme (Thymus vulgaris L.) to the elicitors of coronatine, methyl jasmonate and cyclodextrin in different levels of supplying moisture under farm conditions

Document Type : Original Article

Authors

1 PhD student in Agronomy Department, Faculty of Agriculture, University of Birjand, Iran

2 Faculty member, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Birjand, Birjand, Iran

3 Plant and Environmental Stresses Research Group, University of Birjand, Birjand, Iran

Abstract

Introduction
Medicinal plants contain rich storages of secondary metabolites or active compounds which are strongly affected by environmental factors, especially drought stress, and also by hormones and their derivatives. Thyme genus has more than 215 species in the world, of which 18 species have been identified in Iran. Thymus vulgaris is one of the main medicinal plants that is cultivated in the most countries. Its main components include Thymol and Carvacrol which have antibacterial, antifungal and antioxidant activity and these compounds can be effective in reducing the symptoms of COVID-19. The levels of moisture supply and elicitors like Coronatine (COR), Methyl Jasmonate (MJ) and Cyclodextrin (CYC) can be effective on the quantitative and qualitative yield.
Materials and methods
This investigation was conducted as split plot based on a randomized complete block design (RCBD) with three replications in the farm of Research Station of Khorasan Razavi Agricultural and Natural Resources Education and Research Center which is placed at 5th km Southeast of Mashhad in Iran. The main plots included three levels of moisture supply (at 40%, 65% and 90% field capacity (FC)) and the sub plots were elicitor levels included (1) control without elicitor, (2) 150 µM cyclodextrin (CYC) (3) 75 nM coronatine (COR) + 150 µM CYC (4) 150 nM COR + 150 µM CYC (5) 150 µM methyl jasmonate (MJ) + 150 µM CYC (6) 300 µM MJ + 150 µM CYC. The seeds were sowed in pro-trays which were filled from peat moss on 24 March 2019. After the germination, two seedlings were held in each cell of portray for transplanting to the field. The seedlings were transplanted to each point of cultivation on 24 May 2019. The treatments of the moisture supply were applied 45 days after transplanting the seedlings. In order to apply moisture treatments, at the first, FC was determined by farm and pressure plate method. With sampling the soil of plots (mean of two sample 0-15 cm and 15-30 cm) for several times, was determined the irrigation date. Elicitor treatments were applied two times; the first was 45 days after transplanting and the second was two months later. The plants were harvested two weeks after the last elicitor spraying.
Results and discussion
The largest crown diameter was observed in interaction of elicitor treatments and the levels of moisture supply at 90% FC.The highest leaf proline content (from 5.5 to 5.8 µmol g-1 fresh leaves) was observed in interaction of 4 levels with elicitor treatment (levels 3, 4, 5 and 6) at the level of moisture supply 40% FC that placed in one group. The highest and lowest electrolyte leakage (47.6% and 22.1%, respectively) were related to interaction of the control treatment at the level of moisture supply of 40% FC and the treatment of 150 μM MJ + 150 μM CYC at the level of moisture supply 90% FC. The highest yield of dry herb and leaves and essential oil (5.64 and 3.2 t ha-1 and 65.9 kg ha-1 respectively) was obtained under moisture supply at 90% FC. Except the percentage of essential oil, there was a significant correlation between all measured traits including crown diameter, height, electrolyte leakage, proline content, relative water content of leaf, fresh and dry yield of herb, dry leaf and stem yield and essential oil yield. Although the interaction of the treatments affected some physiological traits, but the quantitative and qualitative yield of the plants were affected by moisture levels.
Conclusion
As a result, drought stress and elicitors can effect on plant performance; morphological, physiological and chemical dynamics. In this study, suppling moisture levels had an impotent effect on biological yield and this indicator caused to increase essential oil yield. Elicitors and interaction of treatments had a significant effect on some physiological characteristics.

Keywords

Main Subjects

References
Alfermann, A.W., 2018. Production of natural products by plant cell and organ cultures. In: Roberts, J.A. (ed.), Annual Plant Reviews book series, Functions and Biotechnology of Plant Secondary Metabolites. 39, 381-399. https://doi.org/10.1002/9781119312994.apr0422
Alikhani, O., Abbaspour, H., Safipour afshar, A., Motevalizadeh Kakhaki, A.R., 2019. Effects of methyl jasmonate on cadmium accumulation, antioxidant capacity and some physiological traits of wheat seedlings (Triticum aestivum L.). Journal of Plant Research (Iranian Journal of Biology), 32(4), 886-897. [In Persian with English Summary].
Asada, K., 1999. The water-water cycle in chloroplasts: scavenging of active oxygen and dissipation of excess photons. Annual Review of Plant Physiology and Plant Molecular Biology. 50, 601–639. https://doi.org/10.1146/annurev.arplant.50.1.601
Askary, M., 2017. Evaluation of some morpho-physiological and phytochemical traits of Thymus vulgaris L. and Thymus daenensis L. species in drought conditions and the use of manure. Ph.D. Thesis, Department of Agronomy and Plant Breeding, University of Birjand, Birjand, Iran [In Persian].
Askary, M., Behdani, M.A., Parsa, S., Mahmoodi, S., Jami Al-Ahmadi, M., 2018. Water stress and manure application affect the quantity and quality of essential oil of Thymus daenensis and Thymus vulgaris. Industrial Crops and Products, 111, 336-344. https://doi.org/10.1016/j.indcrop.2017.09.056
Baenas, N., Garcia-Viguera, C., Moreno, D.A., 2014. Elicitation: A tool for enriching the bioactive composition of foods. Molecules, 19, 13541–13563. https://doi.org/10.3390/molecules190913541 
Bandurska, H., Stroin-ski, A., Kubis´, J., 2003. The effect of jasmonic acid on the accumulation of ABA, proline and spermidine and its influence on membrane injury under water deficit in two barley genotypes. Acta Physiologiae Plantarum., 25, 279–285. https://doi.org/10.1007/s11738-003-0009-0
Barker, D.G., Sullivan, C.Y., Moser, L.E, 1993. Water deficit effects on osmotic potential, cellwall elasticity, and proline in five forage grasses. Agronomy Journal. 85, 270-275. https://doi.org/10.2134/agronj1993.00021962008500020020x 
Bates, L.S., Waldern, R.P., Tear, I.D., 1973. Rapid determination of free proline for water stress studies. Plant and Soil. 39, 207-207.
Blackman, S.A., Obendorf, R.L., Lepold, A.C., 1995. Desiccation tolerance in developing soybean seeds: The role of stress proteins. Plant Physiology, 93, 630-638. https://doi.org/10.1111/j.1399-3054.1995.tb05110.x
Blum, A. 1988. Plant Breeding for stress Environments. CRC Press. Inc. 231p. https://doi.org/10.1201/9781351075718
Blum, A., 1996. Crop response to drought and the interpretation adaptation. Plant Growth Regulation. 20, 135-148. (1996). https://doi.org/10.1007/BF00024010
Cabuslay, G.S., Ito, O., Alejar, A.A., 2002. Physiological evaluation of responses of rice (Oryza sativa L.) to water deficit. Plant Science. 163, 815-827. https://doi.org/10.1016/S0168-9452(02)00217-0
Castrillo, M., Turujillo, I., 1994. Ribulose-1, 5 bisphosphate carboxylase activity and chlorophyll and protein contents in two cultivares of French bean plants under water stress and rewatering. Photosynthetica. 30, 175-181.
Chaves, M.M., Pereira, J.S., Maroco, J., Rodriques, M.L., Ricardo, C.P.P., Osorio, M.L., Carvatho, I., Faria, T., Pinheiro, C., 2002. How plants cope with water stress in the field photosynthesis and growth? Annuals of Botany. 89, 907-916. https://doi.org/10.1093/aob/mcf105
Gallego, A., Imseng N., Bonfill M., Cusido R.M., Palazon J., Eibl R., Moyano E., 2015. Development of a hazel cell culture-based paclitaxel and baccatin III production process on a benchtop scale. Journal of Biotechnology. 195, 93–102. https://doi.org/10.1016/j.jbiotec.2014.12.023
Ghaderi, A.A., 2015. Effects of salicylic acid and jasmonic acid on morphological and physiological traits of medicinal plant thymus vulgaris under drought stress (Thymus vulgaris L.). MSc dissertation. Department of Horticulture and Landscape. University of Zabol. Zabol, Iran. [In Persian].
Ghassemian, M., Lutes, J., Chang, H., Lange, I., Chen, W., Zhu, T., Wang, X., Lange, B.M., 2008. Abscisic acid-induced modulation of metabolic and redox control pathways in Arabidopsis thaliana. Phytochemistry. 69, 2899–2911. https://doi.org/10.1016/j.phytochem.2008.09.020
Hashemi, S., 2016. Evaluation the effect of coronatine pretreatment on stress zinc oxide synthesized by olive and aleo vera on soybean plant. Ph.D. Thesis, Department of Biology, Shahid Bahonar University of Kerman. Kerman, Iran. [In Persian].
Hornok, L., 1988. Effect of environmental factors on the production of some essential oil plants. In: Lawrence, B.M., Mookherjee, D.B., Willis, B.J. (eds.), Flavors and Fragrances: A World Perspective. Elsevier, Amsterdam. pp. 129-140
Jamzad, Z., 2009. Thymus and Satureja species of Iran. Research Institute of Forests and Rangelands. 171p. [In Persian].
Kafi, M., Borzoee, A., Salehi, M., Kamandi, A., Masoumi, A., Nabati, J., 2014. Physiology of Environmental Sstresses in Plants. Jahad Daneshgahi Press, Mashhad, Iran 502P. [In Persian].
Klarzynski, O., Friting, B., 2001. Stimulation of plant natural defenses. Comptes Rendus Biologies Academic Science. 324, 953–963. https://doi.org/10.1016/s0764-4469(01)01371-3
Lebaschy, M.H., Sharifi Ashoorabadi, E., 2004. Growth indices of some medicinal plants under different water stresses. Iranian Journal of Medicinal and Aromatic Plants. 20, 249-261. [In Persian with English summary].
Letchamo, W., Gosselin, A., 1996. Transpiration, essential oil glands, epicuticular wax and morphology of Thymus vulgaris are influenced by light intensity and water supply. Journal of Horticultural Science, 71, 123-134. https://doi.org/10.1080/14620316.1996.11515388
Levitt, J., 1980. Responses of Plants to Environmental Stresses. Vol. II: Water, radiation, salt, and other stresses. Academic Press, United Kingdom
Littleson, M.M., Russell, C.J., Frye, E.C., Ling, K.B., Jamieson, C., Watson, A.J.B., 2016. Synthetic approaches to coronafacic acid coronamic acid and coronatine. Synthesis. Vol. 48. https://doi.org/10.1055/s-0035-1562552
Dell-Valle, E.M., 2003. Cyclodextrins and their uses: a review. Process Biochemistry. 39, 1033–1046. https://doi.org/10.1016/S0032-9592(03)00258-9
Marin, M., Budimir, S., Janosevic, D., Marin, P., Duletic, S., Grbic, M., 2008. Morphology, distribution, and histochemistry of trichomes of Thymus lykae Degen and Jav. (Lamiaceae). Archives of Biological Sciences. 60, 667–672. https://doi.org/10.2298/ABS0804667M
Miranshahi, B., Sayyari, M., 2016. Methyl jasmonate mitigates drought stress injuries and affects essential oil of summer savory. Journal of Agricultureal Science and Technology (JAST). 18, 1635-1645.
Mittler, R., 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science. 7, 405–410. https://doi.org/10.1016/S1360-1385(02)02312-9.
Moein Alishah H., Heidari R., Hassani A., Asadi Dizaji A., 2006. Effect of water stress on some morphological and biochemical characteristics of purple Basil (Ocimum basilicum L.). Journal of Biological Science. 6, 763-767. https://doi.org/10.3923/jbs.2006.763.767
Mohamed, H.I., Latif, H.H., 2017. Improvement of drought tolerance of soybean plants by using methyl jasmonate. Physiology and Molecular Biology of Plants. 23, 545–556. https://doi.org/10.1007/s12298-017-0451-x
Mohammadi, H., Amirikiaa, F., Ghorbanpour, M., Fatehic, F., Hashempou, H., 2019. Salicylic acid induced changes in physiological traits and essential oil constituents in different ecotypes of Thymus kotschyanus and Thymus vulgaris under well-watered and water stress conditions. Industrial Crops and Products. 129, 561-574. https://doi.org/10.1016/j.indcrop.2018.12.046
Moradi, P., 2018.The impact of drought stress on growth and hormone alterations in Thyme plant. journal of plant process and function, 6,311-322.
Moradi, P., 2018. The impact of drought stress on growth and hormone alterations in Thyme plant . Journal of Plant Process and Function. 6(19), 311-322. [In Persian with English summary].
Munns, R., 1993. Physiological process limiting plant growth in saline soil: some dogmass and hypotheses. Plant, Cell and Environment.16, 15-24. https://doi.org/10.1111/j.1365-3040.1993.tb00840.x
Nikolić, M., Glamočlija, J., Ferreira, I.C.F.R., Calhelha, R.C., Fernandes, Â., Marković, T., Marković, D., Giweli, A., Soković, M., 2014. Chemical composition antimicrobial antioxidant and antitumor activity of Thymus serpyllum L., Thymus algeriensis Boiss. and Reut and Thymus vulgaris L. essential oils. Industrial Crops and Products, 52. 183–190. https://doi.org/10.1016/j.indcrop.2013.10.006.
Oksman-Caldentey, K.M., Inzé, D., 2004. Plant cell factories in the post-genomic era: new ways to produce designer secondary metabolites. Trends in Plant Science. 9, 433-440. https://doi.org/10.1016/j.tplants.2004.07.006.
Onrubia, M., A., 2012. Molecular Approach to Taxol Biosynthesis. Ph.D. Thesis, Universitat Pompeu Fabra, Barcelona, Spain.
Petropoulos, S.A., Dimitra, D., Polissiou, M.G., Passam, H.C., 2008. The effect of water deficit stress on the growth, yield and composition of essential oils of parsley. Scientia Horticulturae, 115, 393-397. https://doi.org/10.1016/j.scienta.2007.10.008.
Prasanth Reddy, V., Ravi Vital, K., Varsha, P.V., Satyam, S., 2014. Review on Thymus vulgaris traditional uses and pharmacological properties. Medicinal and Aromatic Plants, 3: 164. https://doi.org/10.4172/2167-0412.1000164
Rassam, G., Dadkhah, A., Khoshnood-Yazdi, A., 2014. Evaluation of water deficit on morphological and physiological traits of hyssop (Hyssopus officinalis L.). Iranian Jouran of Agronomy Sciences, 5(10), 1-12. [In Persian with English summary].
Ritchie, S.W., Nguyen, H.T., Holaday, A.S., 1990. Leaf water content and gas-exchange parameters of two wheat genotypes differing in drought resistance. Crop Science. 30, 105-111. https://doi.org/10.2135/cropsci1990.0011183X003000010025x
Rubio, V., Bustos, R., Luisa, M.L., Irigoyen, X., Cardona-Lopez, Rojas-Triana, M., Paz-Ares, J., 2009. Plant hormones and nutrient signaling. Plant Molecular Biology. 69, 361–373. https://doi.org/10.1007/s11103-008-9380-y
Sabater-Jara, A.B., Onrubia, M., Moyano, E., Bonfill, M., Palazón, J., Pedreño, M.A., Cusidó, R.M., 2014. Synergistic effect of cyclodextrins and methyl jasmonate on taxane production in Taxus x media cell cultures. Plant Biotechnology Journal. 12, 1075–1084. https://doi.org/10.1111/pbi.12214
Sanchez, F.J., Manzanares, M., Andres,E.F., Tenorio,J.L.,and Ayerbe,. L., 1998. Turgor maintenance, osmotic adjustment and soluble sugar and proline accumulation in 49 pea cultivars in response to water stress. Field Crops Research. 59, 225-235. https://doi.org/10.1016/S0378-4290(98)00125-7
Seleiman, M.F., Al-Suhaibani, N., Ali, N., Akmal, M., Alotaibi, M., Refay, Y., Dindaroglu, T., Ab-dul-Wajid, H.H., Battaglia, M.L., 2021. Drought stress impacts on plants and different approaches to alleviate its adverse effects. Plants. 10, 259. https://doi.org/10.3390/plants10020259
Sharma, A., Shahzad, B., Kumar, V., Kohli, S.K., Sidhu-Gagan, P.S., Bali-Aditi S., Handa, N., Kapoor, D., Bhardwaj, R., Zheng, B., 2019. Phytohormones regulate accumulation of osmolytes under abiotic stress biomolecules. 9, 285. https://doi.org/10.3390/biom9070285
Shi, D., Sheng, Y., 2005. Effects of various salt-alkaline mixed stress conditions on sunflower seedling and analysis of their stress factors. Environmental and Experimental Botany. 54, 8-21. https://doi.org/10.1016/j.envexpbot.2004.05.003
Soltani, A., Faraji, A., 2011. Soil, Water and Plant Relationship. Jahad Daneshgahi Press, Mashhad, Iran. 246. [In Persian].
Sreevalli, Y., Baskaran, K., Chandrashekara, R., kuikkarni, R., SuShil Hasan, S., Samresh, D., Kukre, J., Ashok., A., Sharmr Singh, K., Srikant, S., and Rakesh, T., 2001. Preliminary observations on the effect of irrigation frequency and on yield and alkaloid concentration in petriwinkle. Journal of Medicine and Aromatic Plant Sciences. 22, 356-358.
Stahl-Biskup, E., Sáez, F., 2002. Thyme: the Genus Thymus. London: Taylor & Francis.
Sueldo, R.J., Invernati, A., Plaza, S.G., Barassi, C.A., 1996. Osmotic stress in wheat seedlings: effects on fatty acid composition and phospholipid turnover in coleoptiles. Cereal Research Communications. 24(1), 77–84. http://www.jstor.org/stable/23783920
Taiz, L. Zeiger, E., Moller, I.M., Murphy, A., 2015. Plant Physiology and Development. 6th Edition, Sinauer Associates, Sunderland, CT
Uppalapati, S.R., Ayoubi, P., Weng, H., Palmer, D.A., Mitchell, R.E., Jones, W., Bender, C.L., 2005. The phytotoxin coronatine and methyl jasmonate impact multiple phytohormone pathways in tomato. The Plant Journal. 42, 201–217. https://doi.org/10.1111/j.1365-313X.2005.02366.x
Wang, B., Li, Z., Eneji, E.A., Tian, X., Zhai, Z., Li, J., Duan, L., 2008. Effects of coronatine on growth gas exchange traits chlorophyll content antioxidant enzymes lipid peroxidation in maize (Zea mays L.) seedling under simulated drought stress. Plant Production Science. 11, 283-290. https://doi.org/10.1626/pps.11.283
Wani, A R., Yadav, K., Khursheed, A., Rather, M.A., 2020. An updated and comprehensive review of the antiviral potential of essential oils and their chemical constituents with special focus on their mechanism of action against various influenza and coronaviruses. Microbial Pathogenesis. 152. https://doi.org/10.1016/j.micpath.2020.104620
Zare Dehabadi, S., 2013. Evaluation the effects of coronatine and sSodium nitroprusside pretreatment in reduction of oxidative stress induced by arsenic as heavy metal and change in some secondary metabolites in Ocimum basilicum L. Ph. D. Thesis, Department of Biology, Shahid Bahonar University of Kerman, Kerman, Iran [In Persian].
Environmental Stresses in Crop Sciences
Volume 16, Issue 2
Open Access Policy
May 2023
Pages 467-485
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  • Receive Date: 09 October 2021
  • Revise Date: 20 November 2021
  • Accept Date: 04 December 2021
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