Document Type : Original Article

Authors

1 Ph.D. Student, Department of Agronomy and Plant Breeding, Karaj Branch, Islamic Azad University, Karaj, Iran

2 Professor, Department of Agronomy and Plant Breeding, Karaj Branch, Islamic Azad University, Karaj, Iran

3 Assistant Professor, Maize and Forage Crops Research Department, Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

4 Associate Professor, Department of Agronomy and Plant Breeding, Karaj Branch, Islamic Azad University, Karaj, Iran

5 Assistant Professor, Department of Agronomy and Plant Breeding, Karaj Branch, Islamic Azad University, Karaj, Iran

Abstract

Introduction

Lack of irrigation water resources has been identified as the most important problem in forage production. Therefore, to increase the productivity of crop production using limited water resources, it is necessary to pay attention to the cultivation of drought-tolerant crops. Sorghum has a high resistance to abiotic stresses and can perform well in comparison with other summer crops. Irrigation and fertilization are not only costly but also are of the most important factors affecting the quantity and quality of forage crops. Therefore, the present study was conducted to evaluate the effects of different levels of drought stress and nitrogen fertilizer on the sorghum forage yield and quality, and water use efficiency.



Materials and methods

This study was conducted as split-plots based on a randomized complete block design with three replications in Karaj, Iran, during the 2018 growing season. Drought stress at three levels (no-stress, moderate and severe stress; including the supply of 100, 75, and 50% soil moisture deficit, respectively) as the main factor and nitrogen fertilizer application from urea source at four levels (0, 150, 300, and 450 kg ha-1) as the sub-factor were evaluated. In all experimental treatments, nitrogen fertilizer was applied in two equal parts, at planting and 5-6 leaf stage. In the present study, drip tape irrigation approach was applied (with a diameter of 16 mm and drip distance of 10 cm). Irrigation cycle was considered constant for all plots and different levels of irrigation water were applied. In order to properly establish the sorghum crops, deficit irrigation regimes were started after 2-4 leaf stage. Sorghum forage was harvested at the milky-dough stage. Data were subjected to two-way analysis of variance (ANOVA) and the difference between treatment means was separated using LSD test. A significance level of 95% was applied by GLM procedure of SAS 9.1.



Results and discussion

The results of ANOVA showed that the main effect of drought stress and nitrogen fertilizer on the forage yield, irrigation water use efficiency (IWUE), plant height, and quality characteristics of sorghum forage (except hemicellulose) was significant (p≤0.01). Also, the interaction effect of drought stress × nitrogen fertilizer on the forage yield, IWUE, plant height, and crude protein content at the probability level of 1%, and on other quality characteristics of sorghum forage (except hemicellulose) at the probability level of 5% was significant. The highest dry-matter and protein yield (40.03 and 3.48 t ha-1, respectively) and the maximum plant height (224 cm) were obtained with full irrigation and application of 450 kg nitrogen ha-1, whereas the maximum IWUE for dry matter and protein production (6.793 and 0.672 kg m-3, respectively) was obtained under moderate stress and with the application of 450 kg nitrogen ha-1. By increasing the nitrogen fertilizer application from 0 to 450 kg ha-1 under full irrigation, moderate stress, and severe stress conditions, the dry matter yield increased by 167, 181 and 101%, respectively, protein yield increased by 238, 284 and 174%, respectively, forage protein content increased by 27, 36 and 39%, respectively, and relative feed value increased by 8, 6 and -2%, respectively. Overall, in order to achieve the maximum quantity and quality of forage and the highest water use efficiency in full irrigation and moderate drought stress conditions, application of 450 kg nitrogen ha-1, and in severe stress conditions, application of 300 kg nitrogen ha-1 can be recommended.



Conclusion

Generally, the results illustrated that the treatment of moderate drought stress (supply of 75% soil moisture deficit) with the application of 450 kg nitrogen ha-1, along with saving water and producing high forage yield, among the studied treatments resulted in the highest water use efficiency, DMD, NEL, and RFV, and the minimum ADF and NDF, can be recommended as the superior treatment for sorghum forage. In case of severe limitation of irrigation water resources, supply of 50% soil moisture deficit (severe drought stress) along with application of 300 kg nitrogen ha-1 can be recommended.

Keywords

Main Subjects

Abid, M., Mansour, E., Ben Yahia, L., Bachar, K.H., Ben Khaled, A., Ferchichi, A., 2016. Alfalfa nutritive quality as influenced by drought in South-Eastern Oasis of Tunisia. Italian Journal of Animal Science. 15, 334-342.
Afshar, R.K., Jovini, M.A., Chaichi, M.R., Hashemi, M., 2014. Grain sorghum response to arbuscular mycorrhiza and phosphorus fertilizer under deficit irrigation. Agronomy Journal. 4, 1212-1218.
Anjum, S.A., Ashraf, U., Tanveer, M., Khan, I., Hussain, S., Shahzad, B., Wang, L.C., 2017. Drought induced changes in growth, osmolyte accumulation and antioxidant metabolism of three maize hybrids. Frontiers in Plant Science. 8, 69.
Ashoori, N., Abdi, M., Golzardi, F., Ajalli, J., Ilkaee. M.N., 2021. Forage potential of sorghum-clover intercropping systems in semi-arid conditions. Bragantia, 80, e1421.
Baghdadi, A., Balazadeh, M., Kashani, A., Golzardi, F., 2017a. Effects of pre-Sowing treatments and nitrogen rates on quantitive and qualitative characteristics of silage maize SC 704. Journal of Crop Production, 9, 103-120. [In Persian with English Summary].
Baghdadi, A., Balazadeh, M., Kashani, A., Golzardi, F., Gholamhoseini, M., Mehrnia, M., 2017b. Effect of pre-sowing and nitrogen application on forage quality of silage corn. Agronomy Research. 15, 11-23.
Bahrololomi, S., Raeini Sarjaz, M., Pirdashti, H., 2019. The effect of drought stress on the activity of antioxidant enzymes, malondialdehyde, soluble protein and leaf total nitrogen contents of soybean (Glycine max L.). Environmental Stresses in Crop Sciences. 12, 17-28.
Bakhtiyari, F., Zamanian, M., Golzardi, F., 2020. Effect of mixed intercropping of clover on forage yield and quality. South-Western Journal of Horticulture, Biology and Environment. 11, 49-65.
Balazadeh, M., Zamanian, M., Golzardi, F., Mohammadi Torkashvand, A., 2021. Effects of limited irrigation on forage yield, nutritive value and water use efficiency of Persian clover (Trifolium resupinatum) compared to Berseem clover (Trifolium alexandrinum). Communications in Soil Science and Plant Analysis. 52, e1900228.
Bhattarai, B., Singh, S., West, C.P., Ritchie, G.L., Trostle, C.L., 2020. Effect of deficit irrigation on physiology and forage yield of forage sorghum, pearl millet, and corn. Crop Science. 60, 2167-2179.
Fried, H.G., Narayanan, S., Fallen, B., 2019. Evaluation of soybean ((Glycine max L.) Merr.) genotypes for yield, water use efficiency, and root traits. Plos One. 14, 1-18.
Ghobadi, R., Shirkhani, A., Jalilian, A., 2015. Effects of Water stress and nitrogen fertilizer on yield, its components, water and nitrogen use efficiency of corn (Zea mays L.) cv. SC. 704. Applied Field Crops Research. 28, 79-87. [In Persian with English Summary].
Golzardi, F., Vazan, S., Moosavinia, H., Tohidloo, G., 2012. Effects of salt and drought stresses on germination and seedling growth of swallow wort (Cynanchum acutum L.). Research Journal of Applied Sciences, Engineering and Technology. 4, 4524-4529.
Golzardi, F., Baghdadi, A., Keshavarz Afshar, R., 2017. Alternate furrow irrigation affects yield and water-use efficiency of maize under deficit irrigation. Crop and Pasture Science. 68, 726-734.
Golzardi, F., Nazari Sh., Rahjoo, V., 2019. Sorghum Cultivation. ETKA Publication. 183p. [In Persian].
Heidari, N., Alizadeh, Y., Alizadeh, H., 2019. Investigating the interaction of salinity, drought and nitrogen fertilizer stresses on some physiological traits, yield and yield components of maize (Zea mays L.). Environmental Stresses in Crop Sciences. 12(3), 889-905. [In Persian with English Summary].
Hussaini, M.A., Ogunlela, V.B. Ramalan, A.A., Falaki, A.M., 2008. Mineral composition of dry season maize (Zea mays L.) in response to varying levels of nitrogen, phosphorus and irrigation at Kadawa, Nigeria. World Journal of Agricultural Sciences. 4, 775-780.
Islam, M.R., Garcia, S.C., Horadagoda, A., 2012. Effects of irrigation and rates and timing of nitrogen fertilizer on silage dry matter yield, proportions of plant fractions of maize and nutritive value and in vitro gas production characteristics of whole crop maize silage. Animal Feed Science and Technology. 172, 125-135.
Jahanzad, E., Jorat, M., Moghadam, H., Sadeghpour, A., Chaichi, M. R., Dashtaki, M., 2013. Response of a new and a commonly grown forage sorghum cultivar to limited irrigation and planting density. Agricultural Water Management. 117, 62-69.
Jahanzad, E., Sadeghpour, A., Hosseini, M.B., Barker, A.V., Hashemi, M., Zandvakili, O. R., 2014. Silage yield and nutritive value of millet-soybean intercrops as influenced by nitrogen application. Agronomy Journal. 106, 1993-2000.
Jahansouz, M.R., Afshar, R.K., Heidari, H., Hashemi, M., 2014. Evaluation of yield and quality of sorghum and millet as alternative forage crops to corn under normal and deficit irrigation regimes. Jordan Journal of Agricultural Sciences. 10, 699-715.
Javadi, H., Saberi, M., Azari Nasrabad, A., Khosravi, S., 2010. The study of amounts and methods of nitrogen application on quantitative and qualitative characteristics of forage sorghum, variety Speedfeed. Iranian Journal of Field Crops Research. 8, 384-392.
Kamaei, R., Faramarzi, F., Parsa, M., Jahan, M., 2019. The effects of biological, chemical, and organic fertilizers application on root growth features and grain yield of sorghum. Journal of Plant Nutrition. 42, 2221-2233.
Kaplan, M., Kara, K., Unlukara, A., Kale, H., Buyukkilic Beyzi, S., Varol, I.S., Kizilsimsek, M., Kamalak, A., 2019. Water deficit and nitrogen affects yield and feed value of sorghum sudangrass silage. Agricultural Water Management. 218, 30-36.
Khelil, M.N., Rejeb, S., Henchi, B., Destain, J.P., 2013. Effects of irrigation water quality and nitrogen rate on the recovery of N fertilizer by sorghum in field study. Communications in Soil Science and Plant Analysis. 44, 2647-2655.
Kjeldahl, J.G.C.T., 1883. Neue methode zur bestimmung des stickstoffs in organischen körpern. Fresenius, Zeitschrift für analytische Chemie. 22, 366-382.
Li, C.X., Zhou, X.G., Sun, J.S., Wang, H.Z., Gao, Y., 2013. Dynamics of root water uptake and water use efficiency under alternate partial root zone irrigation. Desalination and Water Treatment. 52, 2805-2810.
Machicek, J., 2018. Evaluating forage sorghum and pearl millet for forage production and quality in the Texas high plains. M.S. Thesis. West Texas A&M University, Department of Agricultural Sciences, Canyon, TX.
Machicek, J.A., Blaser, B.C., Darapuneni, M., Rhoades, M.B., 2019. Harvesting regimes affect brown midrib sorghum-sudangrass and brown midrib pearl millet forage production and quality. Agronomy. 9, 1-13.
Maranville, J.W., Madhavan, S., 2002. Physiological adaptation for nitrogen use efficiency in sorghum. Plant and Soil. 245, 25-34.
McCuistion, K., Bean, B., McCollum, F.T., 2010. Nutritional composition response to yield differences in brown midrib, non-brown midrib, and photoperiod-sensitive forage sorghum cultivars. Forage and Grazinglands. 8, 1-7.
Meisser, M., Vitra, A., Mosimann, E., Deléglise, C., Buttler, A., 2017. Linking functional plant traits and forage quality under drought conditions. Grassland resources for extensive farming systems in marginal lands, major drivers and future scenarios. Proceedings of the 19th Symposium of the European Grassland Federation, Alghero, 7-10 May, Italy.
Nematpour, A., Eshghizadeh, H.R., Zahedi, M., Ghorbani, G.R., 2020. Millet forage yield and silage quality as affected by water and nitrogen application at different sowing dates. Grass and Forage Science. 75, 169-180.
Ul-Allah, S., Khan, A.A., Fricke, T., Buerkert, A., Wachendorf, M., 2014. Fertilizer and irrigation effects on forage protein and energy production under semi-arid conditions of Pakistan. Field Crops Research. 159, 62-69.
Van Soest, P.J., Robertson, J.B., Lewis, B.A., 1991. Methods for dietary fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science. 74, 3583–3597.
Zhao, D., Reddy, K.R., Kakani, V.G., Reddy, V.R., 2005. Nitrogen deficiency effects on plant growth, leaf photosynthesis, and hyperspectral reflectance properties of sorghum. European Journal of Agronomy. 22, 391-403.
Zou, Y., Saddique, Q., Ali, A., Xu, J., Khan, M. I., Qing, M., Azmat, M., Cai, H., Siddique, K.H.M., 2021. Deficit irrigation improves maize yield and water use efficiency in a semi-arid environment. Agricultural Water Management. 243, 106483.