Document Type : Original Article
Authors
1 Department of Agrotechnology, Varamin Branch Islamic Azad University, Tehran, Iran
2 Department of Agronomy and Plant Breeding, Isfahan(Khorasgan) Branch Islamic Azad University, Isfahan, Iran
Abstract
Introduction
Corn (Zea mays L) is an annual cereal crop adapted to various ecological conditions. Corn with wheat and rice are three strategic agricultural products in the world. Drought stress is the crucial factor to limit the production of corn among abiotic stresses. Drought stress in corn reduces photosynthesis, and consequently grain yield by reducing the leaf chlorophyll level. Many external and internal factors are effective in plant growth. The most important internal factors are hormones and the most important external factors are light and temperature. Hormones regulate Plant Growth Regulators (PGRs) and coordinate the processes occurring in different parts of the plant body. Plant Growth Regulators (PGRs) increase plant tolerance to various environmental stresses, such as drought, salinity, cold, and heat, and this increase in tolerance to environmental stresses depends on the production of transcripts of anti-stress genes, such as heat shock genes and LEA genes. It is possible to divide the products of genes induced under drought stress conditions into two categories: (1) Those playing a direct protective role against stresses; (2) Those controlling gene expression and message transmission. The Rab-17 gene is one of the genes whose expression increases during drought stress following an increase in ABA. This gene belongs to second group LEA proteins. Several gene products identical to Rab17-encoded proteins have been identified in different plants. Studies indicate that LEA proteins act as water-binding molecules in grains and protect other proteins against the negative effects of drought, including ion separation from macro molecules and membrane protection against freezing damage. This study was conducted to examine the effect of the foliar application of proline amino acid and growth regulators, including benzyl adenine, gibberellic acid, as well as the combination of benzyl adenine and gibberellic acid and proline amino acid on the growth, yield and expression of the Rab-17 gene in corn (Zea mays L) (Single Cross 704) under drought stress.
Material and methods
This study was conducted as a split plot on the base of randomized complete block design with three replications in 2015 and 2016. Three irrigation treatments were considered, including after 70 (control), 90, and 100 mm evaporation from the surface of standard Class A evaporation pan as the main agent. Phytohormones BA6, GA (3+ 7), proline amino acid, BA6+ GA (3+ 7) +AA and pure water as control were considered five levels of the sub-factor. The plants were irrigated every 10 days before water stress was applied. Mild and severe stress treatments were applied 45 days after planting and in the 12-leaf stage. Afterward, foliar Plant Growth Regulators (PGRs) and proline were applied in the stem elongation stage of corn.
Results and discussion
The results and analysis of the data indicate that all the measured traits decreased with drought stress; however, the proline content increased by increasing the drought stress. For example, drought stress reduced forage yield by 46%. Corn yield was 1.3 times of the control treatment under severe drought stress with foliar application of proline amino acid. In the genetic experiments, increase of drought stress did not increase the PCR response to the Rab-17 gene due to the increased secretion of abscisic acid (ABA) and susceptibility of single cross 704 to drought stress.
Conclusions
According to the results, the foliar application of proline amino acid and Plant Growth Regulators (PGRs) can improve the growth and yield of corn under drought stress by improving plant physiological and genetic responses. Furthermore, the change in the expression of a number of genes appears to be occurred in response to drought stress. The results indicate that gene expression plays a vital role in the drought tolerance of corn; hence, it is suggested to be highly examined in further studies. It is possible that the current results draw our attention to the effects of tested Plant Growth Regulators (PGRs) on the physiology and genetic contents of corn in drought conditions to use the regulators at the right time to deal with environmental stresses in addition to perform proper management in the field, and to increase the efficiency of roots to uptake nutrients.
Acknowledgments
The authors thank Islamic Azad University, Varamin and the Khorasgan branches, as well as Isfahan University of Technology for providing the university with necessary facilities and performing protein and genetic tests.
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