Environmental Stresses in Crop Sciences

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The growth and physiological response of stevia (Stevia rebaudiana Bertoni) medicinal plant to inoculated with endophytic fungi and spraying of spermidine polyamine under salt stress conditions

Document Type : Original Article

Authors

1 Ph.D. Graduated, Shahrood University of Technology. Shahrood, Iran

2 Associate Professor, Agronomy Department, Shahrood University of Technology. Shahrood, Iran

3 Professor, Agronomy Department, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

Abstract

Introduction
Salinity is one of the environmental abiotic stresses and most important factors limiting the growth and production of plants around the world, which dramatically affect various aspects of plant growth and development through anatomical, morphological and physiological changes (Siringam et al., 2011). Saline environments decrease the growth and yield characteristics of plants and increase some physiological properties such as proline content and electrolyte leakage (Noreen et al, 2010). On the other hand, in the recent years, the role of symbiotic fungi and polyamines in plants tolerance to environmental stress such as saline conditions are pronounced. Morever, due to the importance of medicinal plants, many researches of plant sciences have focused on the various aspects of the application of these plants (Vafadar et al., 2018). Therefore, the present study was conducted to investigate the growth and physiological response of stevia (Stevia rebaudiana Bertoni) medicinal plant to inoculation with endophytic fungi and foliar application of spermidine polyamine under salt stress conditions.

Materials and methods
This experiment was conducted at research greenhouse of Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT) at Sari Agricultural Sciences and Natural Resources University using factorial arrangement based completely randomized design with three replicates in spring and summer of 2016. The treatment consisted of salinity in three levels (0, 6 and 12 dS/m), fungal symbiosis treatments including four levels [non-inoculated (control), inoculation with Piriformospora indica (Pi), inoculated with Trichoderma virens (Trich) and co-inoculation of two fungi (Pi+Trich)] and foliar application of spermidine in three levels (0, 0.75 and 1.5 mM). Seedlings of stevia after inoculation with fungi transfered to adaptation chamber for 40 days and then moved to the greenhouse. Plants were irrigated with tap water until the end of vegetative stage and then irrigated with saline water treatments containing mixture of distilled and Caspian sea water. The Spermidine was foliar applied one week before salinity stress. Two weeks after salinity stress, leaf samples were prepared to measure leaf relative water content (RWC), electrolyte leakage (EL), proline content and soluble sugars. Finally, the plants were removed from the pots and the growth and yield traits were measured.

Results and disscusion
The result showed that in saline conditions, endophytic symbiosis, especially Pi+Trich, increased the stem dry weight (40-64%) and leaf dry weight (44-50%), relative water content (5-30%) and proline content (40-64%), and reduced EL (11-20%). Also spraying 0.75 mM spermidine signiticantly increased both leaf dry weight and plant height. Fungal symbiosis, especially Pi+Trich, and spermidine 0.75 mM resulted in an increase in the RWC. Also, spraying with polyamine spermidine at both concentrations of 0.75 and 1.5 mM increased soluble sugars and inoculated with endophytic fungi, particularly co-inoculation of two fungi, led to a reduction in the content of sugar (17%) in the stevia leaf. At the most levels of salinity and fungal treatments, spermidine, especially at the rate of 0.75 mM, led to increase in stem diameter (10-35%) and leaf area (35-46%).

Conclusion
In general, the results of the present study indicated a negative effect of salinity on the growth and physiological characteristics of the stevia plants. However, inoculation of endophytic fungi, particularly co-inoculation of Pi and Trich, improved the growth and physiological parameters and ameliorated adverse effects of salinity in stevia plants. Morever, the spermidine (especially 0.75 mM) induced salt stress tolerance in stevia plants and showed a synergetic effects with endophytic fungi in terms of the mentioned parameters.

Acknowledgements
The authors wish to acknowledge Shahrood University of Technology (Shahrood, Iran), Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), and Sari Agricultural Sciences and Natural Resources University (Sari, Iran) for financial support of this study.

Keywords

References
Abd_Allah, E.F., Abeer Hashem, A.A., Alqarawi, A., Bahkali H., Alwhibi, M.S., 2015. Enhancing growth performance and systemic acquired resistance of medicinal plant (Sesbania sesban L.) Merr using arbuscular mycorrhizal fungi under salt stress. Saudi Journal of Biology Sciences. 22, 274-283.
Abdel Aziz Nahed, G., Taha Lobna, S., Ibrahim Soad, M.M., 2009. Some studies on the effect of putrescine, ascorbic acid and thiamine on growth, flowering and some chemical constituents of gladiolus plants at Nubaria. Ozean Journal of Applied Sciences. 2, 169-179.
Abeer, H., Alterami Salwa, A., Alqarawi, A.A., Elsayed Fathi., A.A., 2016. Arbuscular mycorrhizal fungi enhance basil tolerance to salt stress through improved physiological and nutritional status. Pakistan Journal of Botany. 48, 37-45.
Allen, D.J., Ort, D.R., 2001. Impacts of chilling temperatures on photosynthesis in warm-climate plants. Trends in Plant Science. 6, 36-41.
Amraee Tabar, S., Ershadi, A., Robati, T., 2016. The Effect of Putrescine and Spermidine on Drought Tolerance of Almond and Peach. Agricultural Crop Management. 18(1), 203- 218. [In Persian with English Summary].
Auge, R.M., Stodola, A.J.W., Times, J.E., Saxton, A.M., 2001. Moisture retention properties of a mycorrhizal soil. Journal of Plant and Soil. 230, 87-97.
Bachrach, U., 2005. Naturally occurring polyamines: interaction with macro-molecules. Current Protein Peptide Science. 6, 559-566.
Bahari Saravi, H., Gholami, A., Pirdashti, H., Baradaran Firouzabadi, M., Asghari, H.R., 2019. The growth and physiological response of stevia (Stevia rebaudiana Bertoni) medicinal plant to inoculated with endophytic fungi and spraying of spermidine polyamine under salt stress conditions. Journal of Plant Process and Function. 8(33), 47-64. [In Persian with English Summary].
Bahari Saravi, H., Pirdashti, H., Yaghoubian, Y., 2017. Response of chlorophyll fluorescence and physiological parameters of basil (Ocimum basilicum L.) to plant growth promoting rhizobacteria (PGPR) under salinity stress. Journal of Plant Process and Function. 6 (19), 89-104. [In Persian with English Summary].
Baniasadi, F., Saffari, V.R., Maghsoudi Moud, A.A., 2015. Effect of putrescine and salinity on morphological and biochemical traits and pigment content of marigold plant (Calendula officinalis L.). Journal of Science and Technology of Greenhouse Culture. 6 (1), 125- 134. [In Persian with English Summary].
Bates L, S., Waldren, R.P., Teare, I.D., 1973. Rapid determination of free proline for water stress studies. Plant and Soil. 39, 205-207.
Ben-Asher, J., Tsuyuki, I., Bravdo, B.A., Sagih, M., 2006. Irrigation of grape vines with saline water: Leafarea index, stomatal conductance, transpiration and photosynthesis. Agricultural Water Management. 83, 13-21.
Bewley, J.D., 1979. Physiological aspects of desiccation tolerance. Annual Review of Plant Physiology. 30, 195-238.
Bhosale, K.S., Shinde, B.P., 2011. Influence of arbascular mycorhizal fungi on proline and chlorophyll content in Zingeber officinale grown under water stress. Indian Journal of Fundemental and Applied Life Sciences. 1, 172-176.
Bierman, B., Linderman, R.G., 1980. Quantifying vesicular–arbuscular mycorrhizae: a proposed method towards standardization. New Phytologist. 87, 63 – 67.
Delauney, A.J., Verna, D.P.S., 1993. Proline biosynthesis and osmoregulation in plants. Plant Journal. 4, 215-223.
Farooq, M., Wahid, A., Lee, D.J., 2009. Exogenously applied polyamines increase drought tolerance of rice by improving leaf water status, photosynthesis and membrane properties. Acta Physiologiae Plantarum. 31, 937-945.
Farooq, S., Azam, F., 2006. The use of cell membrane stability (CMS) technique to screen for salt tolerant wheat varieties. Journal of Plant Physiology. 163, 629-637.
Feng, G., Zhang, F.S., Li, X.L., Tian, C.Y., Tang, C., Rengel, Z., 2002. Improved tolerance of maize plants to salt stress by arbuscular mycorrhiza is related to higher accumulation of soluble sugars in roots. Mycorrhiza. 12, 185–190.
Fricke, W., Peter, W.S., 2002. The biophysics of leaf growth in salt-stressed barley. A Study at the cell level. Plant Physiology.1. 374–388.
Gad, N., 2005. Interactive effect of salinity and cobalt on tomato plants II-Some physiological parameters as affected by Cobalt and Salinity. Research Journal of Agriculture and Biological Sciences. 1, 270-276.
Garg, N., Manchanda, G., 2009. Role of Arbuscular Mycorrhizae in the Alleviation of Ionic, Osmotic and Oxidative Stresses Induced by Salinity in (Cajanus cajan L.) Mill sp. (pigeon pea). Journal Agronomy and Crop Science. 195, 110–123.
Gharineh, M.H., Nadian, H., Fathi, G., Siadat, A., Maadi, B., 2009. Role of arbuscular mycorrhizae in development of salt-tolerance of Trifolium alexandrinum plants under salinity stress. Journal of Food, Agriculture and Environment. 7, 432-437.
Ghorbani, A, Razavi, S. M, Ghasemi, V., and Pirdeshti, H. 2019. Effects of Endophyte Fungi Symbiosis on Some Physiological Parameters of Tomato Plants Under 10 Day Long Salinity Stress. Journal of Plant Process and Function. 7 (27), 193-208. [In Persian with English Summary].
Goyal, S.K., Samsher, R., Goyal, R.K., 2010. Stevia (Stevia rebaudiana) a bio-sweetener: A review. International Journal of Food Sciences and Nutrition. 61, 1-10.
Hussein, M., Nadia, M., EL-Gereadly, H.M., EL-Desuki, M., 2006. Role of putrescine in resistance to salinity of pea plants (Pisum sativum L.). Applied Science Research. 2, 598-604.
Kadian, N., Yadav, K., Badda, N., Aggarwal, A., 2013. AM fungi ameliorates growth, yield and nutrient uptake in Cicer arietinum L. Under salt stress. Russian Agricultural Sciences. 39, 321-329.
Kaur-awhney, R., Tiburcio, A., Altabella, T., Galton, W., 2003. Polyamines in plants: An overview. Journal of Cell and Molecular Biology. 2, 1-12.
Kerepesi, I., Galiba, G., 2000. Osmotic and salt stress-induced alteration in soluble carbohydrate content in wheat seedlings. Crop Science. 40, 482-487.
Khalvandi, M., Amerian, M. R., Baradaran, M., and Gholami, A. 2017. Piriformospora indica symbiotic effect on the quantity and quality of essential oils and some physiological parameters of peppermint (Mentha piperita) under salt stress. Journal of Plant Process and Function. 6 (21), 169-184. [In Persian with English Summary].
Khan, A.S., Zora, S., Abbasi, N.A., 2007. Pre-storage putrescine application suppresses ethylene biosynthesis and retards fruit softening during low temperature storage in Angelino plum. Postharvest Biology and Technology. 46, 36-46.
Levitt, J., 1980. Responses of Plants to Environmental Stresses. Academic Press, New York, 607p.
Liu, J.H., Kitashiba, H., Wang, J., Ban, Y., Moriguchi, T., 2007. Polyamines and their ability to provide environmental stress tolerance to plants. Plant Biotechnology. 24, 117-126.
Lutts, S., Kinet, J.M., Bouharmont, J., 1995. Changes in plant response to NaCl during development of rice (Oryza sativa L.) varieties differing in salinity resistance. Journal of Experimental Botany. 46, 1843–1852.
Mahgoub, M.H., Abd El Aziz, N.G., Mazhar, M. A., 2011. Response of Dahilia pinnata L. plant to foliar spray with putrescine and thiamine on growth, flowering and photosynthetic pigments. American Journal of Agricultural Environmental Science. 10, 769-775.
Mahros, K. M., El-Saady, M.B., Mahgoub, M. H., Afaf, M.H., El-Sayed, M.I., 2011. Effect of putrescine and uniconazole treatments on flower characters and photosynthetic pigments of Chrysanthemum indicum L. Plant. Journal of American Science. 7, 399-408.
Manchanda, G., Garg, N., 2008. Salinity and its effects on the functional biology of legumes. Acta Physiologia Plantarum. 30, 595-618.
Oelmuller, R., Sherameti, I., Tripathi, S., Varma, A., 2009. Piriformospora, a cultivable root endophyte with multiple biotechnological applications. Symbiosis. 49, 1-17.
Omokolo, N.D., Tsala, N.G., Djocgoue, P.F., 1996. Changes in carbohydrate, amino acid and phenol content in cocoa pods form three clones after infection with Phytophthora megakarya Bra. And Grif. Annales Botanici Fennici. 77, 153-158.
Orcutt, D.M., Nilsen, E.T., 2000. Physiology of Plants under stress soil and biotic factors. John Wiley and Sons Inc. KA/PP, p,177-235.
Parida, A.K., Das, A.B., 2005. Salt tolerance and salinity effects on plants: A review. Ecotoxicology and Environmental Safety. 60, 324-349.
Pirdashti, H., Yaghoubian, Y., Mohammadi Goltapeh, E., Hosseini, S.J., 2012. Effect of mycorrhiza-like endophyte (Sebacina vermifera) on growth, yield and nutrition of rice (Oryza sativa L.) under salt stress. Journal of Agricultural Technology. 8, 1651-1661.
Ritchie, S.W., Nguyen, H.T., 1990. Leaf water content and gas exchange parameters of two wheat genotypes differing in drought resistance. Crop Science. 30, 105-111.
Sanchez-Rodrıguez, E., Rubio-Wilhelmi, M., Cervilla, L.M., Blasco, B., Rios, J.J., Rosales, M.A., Romero, L., Ruiz, J.M., 2010. Genotypic differences in some physiological parameters symptomatic for oxidative stress undermoderate drought in tomato plants. Plant Science. 178, 30–40.
Seraj, F., Pirdashti, H.; Yaghoubian, Y., Ghasemi Omran, V., 2016. The effect of Piriformospora indica inoculation on salt and drought stress tolerance in Stevia rebaudiana under in vitro conditions. Iranian Journal of plant Biology. 8 (29), 1-20. [In Persian with English Summary].
Singh, L.P., Gill, S.S., Tuteja, N., 2011. Unraveling the role of fungal symbionts in plant abiotic stress tolerance. Plant Signaling and Behavior.6, 175-191.
Siringam, K., Juntawong, N., Cha-um, S., Kirdmanee, C., 2011. Salt stress induced ion accumulation, ion homeostasis, membrane injury and sugar contents in salt-sensitive rice (Oryza sativa L. spp. indica) roots under is oosmotic conditions. African Journal of Biotechnology. 10, 1340-1346.
Sun, Y.M., Horng, C.Y., Chang, F.L., Cheng, L. C., Tian, W.X., 2010. Biosorption of lead, mercury and cadmium ions by Aspergillus tereuss immobilized in a natural matrix. Polish Journal of Microbiology. 59, 37-44.
Syed Sarfraz, H., Muhammad, A., Maqbool, A., Kadambot, H.M., 2011. Polyamines: Natural and engineered abiotic and biotic stress tolerance in plants”. Biotechnology Advances. 29, 300-311.
Talaat, I.M., Bekheta, M.A., Mahgoub, M.H., 2005. Physiological response of periwinkle plants (Catharanthus roseus L.) to tryptophan and putrescine. International Journal of Agriculture and Biology. 7, 210-213.
Tavarini, S., Angelini, L.G., 2013. Stevia rebaudiana Bertoni as a source of bioactive compounds: the effect of harvest time, experimental site and crop age on steviol glycoside content and antioxidant properties. Journal of the Science of Food and Agriculture. 93, 2121-2129
Unal, D., Tuney, I., Sukatar, A., 2007. The role of external polyamines on photosynthetic responses, lipid peroxidation, protein and chlorophyll a content under the UV-A (352 nm) stress in Physica semipinnata. Journal of Photochemistry and Photobiology. 90, 64-68.
Varma, A., Bakshi, M., Lou, B., Hartmann, A., Oelmuller, R., 2012. Piriformospora indica: a novel plant growth-promoting mycorrhizal fungus. Review. NAAS (National Academy of Agricultural Sciences). Agricultural Research. 1, 117–131.
Varma, A., Verma, S., Sudha Sahay, N.S., Bütehorn, B., Franken, P., 1999. P. indica, a cultivable plant growth promoting root endophyte. Applied and Environmental Microbiology. 65, 2741-4.
Velazquez-Robledo, R., Contreras-Cornejo, H. A., Macías-Rodríguez, L., Hernandez-Morales, A., Aguirre, J., Casas-Flores, S., López-Bucio, J., Herrera-Estrella, A., 2011. Role of the 4-phosphopantetheinyl transferase of Trichoderma virens in secondary metabolism, and induction of plant defense responses. Molecular Plant-Microbe Interactions Journal. 24, 1459-1471.
Vierheilig, H., Coughlan, A.P., Wyss, U., Piche, Y., 1998. Ink and vinegar, a simple staining technique for Arbuscular-mycorrhiza fungi. Applied and Environmental Microbiology. 12, 5004-5007.
Yaghoubian, Y., Goltapeh, E.M., Pirdashti, H., Esfandiari, E., Feizias, V., Dolatabadi, H.K., Varma, A.M.H., 2014. Effect of Glomus mosseae and Piriformospora indica on growth and antioxidant defense responses of wheat plants under drought stress. Agricultural Research. 3, 239-245.
Yaghoubian, Y., Siadat, S.A., Telavat, M.R.M., Pirdashti, H., Yaghoubian, I., 2019. Bio-removal of cadmium from aqueous solutions by filamentous fungi: Trichoderma spp. and Piriformospora indica. Environmental Science and Pollution Research. 26, 7863–7872.
Yan-ping, Z., Hai-he, L., Shu-xing, S., Cheng-he, Z., Xin-e, H., 2010. Effect of polyamine priming on seed vigor and seedling chilling tolerance in eggplant. Acta Horticulturae Sinica. 37, 1783–1788.
Zhang, K., John, P.C.L., 2005. Raised level of cyclin dependent kinase after prolonged suspension culture of Nicotiana plumbaginifolia is associated with more rapid growth and division, diminished cytoskeleton and lost capacity for regeneration: implications for instability of cultured plant cells. Plant Cell Tissue and Organ Culture. 82, 295-308.
Zhu, J.K., 2001. Plant salt tolerance. Trends in Plant Science. 6, 66–71.
Environmental Stresses in Crop Sciences
Volume 14, Issue 1
Open Access Policy
April 2021
Pages 249-263
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History
  • Receive Date: 06 July 2019
  • Revise Date: 13 September 2019
  • Accept Date: 02 October 2019
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