Document Type : Original Article

Authors

1 Ph.D. in Agriculture, Department of Agriculture, Zahedan Branch, Islamic Azad University, Zahedan, Iran

2 Assistant Professor, Department of Agriculture, Zahedan Branch, Islamic Azad University, Zahedan, Iran

3 Research Associate Professor, Horticultural crops sciences of department Sistan Agricultural and Natural Resources Research and Education Center, AREEO, Zabol. Iran

4 Assistant Professor, Agro-ecology, Department of Agriculture, Zahedan Branch, Islamic Azad University, Zahedan, Iran

Abstract

In order to study the effect of bio fertilizers and moisture regime on different moisture regimes on the morphophysiological and grain yield of Arg wheat cultivar, an experiment in the form of a split plot based on randomized complete block design with four replications was conducted at Zahak-Zabol Agricultural and Natural Resources Research Station during 2016-2017 crop year.
Irrigation regimes were carried out on three levels as the main plots: irrigation after 45% (control) (S1), 65% (S2) and 85% moisture evacuation from soil (S3); biofertilizer sources were applied in seven levels: control ( F1), application of the locally common fertilizer, application of Azotobacter fertilizer along with the locally common fertilizer (F2), Azotobacter fertilizer with 50% of the locally common fertilizer (F3), Phosphabacter frtilizer with the locally common fertilizer ( F4), phosphabacter fertilizer with 50% of the corresponding chemical control fertilizer (F5), Azotobacter phospha-bacter fertilizer along with the locally common fertilizer (F6), and Azotobacter and phosphabacter along with 50% of the locally common fertilizer (F7), with all these seven serving as the subplots.
The results showed the interactive effect of moisture regime and biofertilizer on the grain yield was significant at 1% level. This was such that in severe drought stress, biofertilizers with a mean of (6237 kg.ha-1) led to the grain yield increase of 38.8%, as compared to the control. Also, the improvement and increase in the 1000-seed weight, and biologic yield and harvest index were 27.6, 17.8 and 29.6 percent, respectively. Overall, the results of this study showed that in the irrigation under drought stress conditions at all stages of development, the use of Azotobacter and phosphabacter biofertilizers could have a positive effect on the grain yield, biologic yield, harvest index and leaf chlorophyll index, thereby improving the negative effects of drought stress.

Keywords

Abdoli, M., Saeidi, M., 2013. Evaluation of water deficiency at the post anthesis and source limitation during grain filling on grain yield, yield formation, some morphological and phonological traits and gas exchange of bread wheat cultivar. Albanian Journal of Agricultural Sciences. 12, 255-265.
Abid, M., Tian, Z., Ata-Ul-Karim, S.T., Liu, Y., Cui, Y., Zahoor, R., Dai, T., 2016. Improved tolerance to post-anthesis drought stress by pre-drought priming at vegetative stages in drought-tolerant and-sensitive wheat cultivars. Plant Physiology and Biochemistry. 106, 218-227.
Afzal, A., Ashraf, M., Asad, S.A., Farooq, M., 2005. Effect of phosphate solubilizing microorganisms on phosphorus uptake, yield and yield traits of Wheat (Triticum aestivum L.) in rainfed area. International Journal of Agriculture and Biology. 7(2), 207-209.
Ahmadi, A., Jodi, M., Tavakoli, A., Ranjbar, M., 2009. Investigation of Yield and Its Related Morphological Traits Responses in Wheat Genotypes under Drought Stress and Irrigation Conditions. Journal of Water and Soil Science. 12, 155-165
Ahmadi, A., Baker, D.A., 1999. Effects of abscisic acid (ABA) on grain filling processes in wheat. Jornal of Plant Growth Regulation. 28, 187-197.
Ahmadi, A., Baker, D.A., 2001. The effect of water stress on grain filling processes in wheat. Journal of Agriculture Siences. 136, 257-269. [In Persian with English Summary]
Ali, M., Jensen, C.R., Mogensen, V.O., Anderson, M.N., 2001. Root Signaling and osmotic adjustment during intetrmittient soil drying sustain grain yield of field grown wheat. Field Crop Research. 62,3 5-52.
Ashraf, M.Y., 1998. Yield and yield components response of wheat (Triticum aestivum L.) genotypes tinder different soil water deficit conditions. Acta Agronomy Hungary. 46, 45-51
Aynehband, A., Tehrani, M., Nabati, DA., 2010. Residue management and N-splitting methods effects on yield, biological and chemical characters of canola ecosystem. Journal of Food, Agriculture and Environment. 8, 317-324.
Baker. N., 2006. Nitrogen uptake characteristics of corn roots at low N concentration as influenced by plant age. Agronomy Journal. 132, 17-19.
Baral, B.R., Adhikari, P., 2013. Effect of Azotobacter on growth and yield of maize. SAARC Journal of Agriculture. 11, 141-147.
Barlow, E.W.R., Donovan, G.R., Lee, J.W., 1983. Water relation and composition of wheat ears grown in liquid culture effect of carbon and nitrogen. Australian Journal of Plant Physiology. 10, 99-108.
Bashan, Y., Holguin, G., de-Bashan, L., 2004. Azospirillum-plant relationships: physiological, molecular, agricultural and environmental advances. Canadian Journal of Microbiology. 50, 521-577.
Basu, M., Bhadoria, P.B.S., Mahapatra, S.C., 2008. Growth, nitrogen fixation yield and kernel quality of peanut in response to lime, organic and inorganic fertilizer levels. Bioresource Technology. 99, 4675-4683.
Biary, A., Golami, A., Asadi Rahmani, H., 2011. Study of the effects of plant growth promoting rhizobacteria of Azotobacter and Azosprillium on growth traits and yield of corn (Zea maize L.). Iranian Journal of Plant and Soil. 25, 1. 1-10. [In Persian with English Summary]
Blum, A., 1998. Improving wheat grain filling under stress by stem reserve mobilization. Euphytica. 100, 77-83.
Blum, A., Ebercon, A., 1976. Genotypic responses in sorghum to drought stress. III. Free proline accumulation and drought resistance. Crop Science. 16, 428-431.
Bostick, W.M.N., Bado, V.B., Bationo, A., Solar, C.T., Hoogenboom, G., Jones, J.W., 2007. Soil carbon dynamics and crop residue yields of cropping systems in the Northern Guinea Savanna of Burkina Faso. Soil and Tillage Research. 93, 138–151.
Brar, G.S., Kar, S., Singh, N.T., 1990. Photosynthetic response of wheat to soil water deficits in the tropics. Journal of Agronomy and Crop Sciences 164, 343-34.
Bredemeier, C., 2005. Laser-induced chlorophyII fluorescence sensing as a tool for site-specific nitrogen fertilizer evaluation under controlled environmental and field condition in wheat and maize. Ph. D. Thesis. Technical University of Munich Germany. pp219.
Bukhat, N.M., 2005. Studies in yield and yield associated traits of wheat (Triticum aestivum L.) genotypes under drought conditions. M.Sc Thesis Department of Agronomy. Sindh Agriculture University, Tandojam, Pakistan.
Chalab-Yani, S., Rashidi, V., 2012. Selection indices in the improvement of wheat grain yield on drought stress conditions. African Journal of Agriculture Research. 7, 1177-1183.
Dogan, R., 2009. The correlation and path coefficient analysis for yield and some yield components of durum wheat (Triticum turgidum L.var. durum) in West Anatolia conditions. Pakistan Journal of Botany. 41, 1081-1089.
Dorostkar, S., Dadkhodaie, A., Heidari, B., 2015. Evaluation of grain yield indices in hexaploid wheat genotypes in response to droughtstress. Archives of Agronomy and Soil Science. 61, 397-413.
Emam, Y., Seghataleslam, M.J., 2005. Crop Yield, Physiology and Processes. Shiraz University Publication. 593. [In Persian]
Emam, Y., Salami, S., Shokofa, A., 2009. Effects of nitrogen levels on yield and yield components of wheat under irrigation and dry land conditions. Iranian Journal of Crop Sciences.7, 321-332. [In Persian with English Summary]
Fageria, N.K., Baligar, V.C., 2005. Enharcing nitrogen use efficincy in cropplants. Advances in Agronomy. 88, 97-185.
Fallah Qazaani, M., Habibi, D., Pazoki, A.R., Khavazi, K., 2012. Effect of some Azotobacter spices and humic acid on auxin hormone production, yield and yield components of wheat under different nitrogen levels. Iranian Journal of Agronomy and Plant Breeding. 8, 97-109. [In Persian with English Summary]
Fanaei, H.R., Galavi, M., Kafi, M., Ghanbari Bonjar, A., Shirani-rad, A.H., 2009. Effect of potassium fertilizer and irrigation on yield and water use efficiency of canola and Indian mustard species (B.napus L. and B. juncea L.). Iranian Journal of Crop Sciences. 11, 273-291 [In Persian with English Summary]
Fischer, R.A., Rees, D., Sayre, K.D., Lu, Z.M., Condon, A.G., Larque-Saavedra, A., 1998. Wheat yield progress associated with higher stomatal conductance and photosynthetic rate and cooler canopies. Crop Science. 38, 1467-1475.
Gastal, F., Nelson, C.J., 1994. Nitrogen use within the growing leaf blade of tall fescue. Plant Physiology. 105-119.
Gerami, F., Aynehband, A., Fateh, E.,, 2012. Effect of Green manures and Nitrogen Fertilizer Levels on Early Growth, Yield and Yield Components of Wheat (Triticum aestivum L.). Journal of Agricultural Science and Sustainable Production. 23, 1-17. [In Persian with English Summary]
Gerhardt, K.E., Greenberg, B.M., Glick B.R., 2006. The role of ACC deaminase in facilitating the phytoremediation of organic, metals and salt. Current Trends in Microbiology. 2, 61-73.
Ghanbari, S., Moosavi, S.Gh., 2016. Grain Yield of Maize Influenced by Plant Growth Promoting Rhizobacteria (PGPR) and Zinc under Water Deficit Stress. 62(3), 21-41.
Glick, B.R., Todorovic, B., Czarny, J., Cheng, Z., Duan, J., McConkey, B., 2007. Promotion of plant growth by bacterial ACC deaminase. Critical Reviews in Plant Sciences. 26 227–242.
Gooding, M.J., Ellis, R.H., Shewry, P.R., Schofield, J.D., 2003. Effects of restricted water availability and increased temperature on the grain filling, 925 drying and quality of winter wheat. Cereal Science. 37, 295–309.
Halim, G.H., Emam, Y., Shakeri, E., 2018. Evaluation of Yield, Yield Components and Stress Tolerance Indices in Bread Wheat Cultivars at Post-anthesis Irrigation Cut-Off. Journal of Crop Production and Processing. 7(4), 121-134. [In Persian with English Summary].
Hassanzadeh, E., Mazaheri, D., Chaichi, M.R., Khavazi, K., 2008. Efficiency of phosphorus solubilizing bacteria and phosphorus chemical fertilizer on yield and yield components of barley cultivar (Karoon Dar Kavir). Pajouhesh and Sazandegi. 77, 111-118 [In Persian with English Summary].
Hooper, S., Heaney, A., Gordon, S., 2000. Phosphorus nutrition in Australian agriculture. Australian Commodities. 72, 341-347.
Kamaei, H.H.R., Eisvand, F., Nazarian, K., 2018. Effects of Planting Date, Bio-fertilizer Containing P Solubilizing Bacteria and Elements Foliar Application of Zinc and Boron on Physiological and Agronomic Traits of Bread Wheat (Aflakcultivar). Iranian Journal of Field Crops Research. 16(1), 165-179. [In Persian with English Summary]
Kandi, A.A., Badawi, M.A., EL-Moursy, S.A., Abdou, M.A., 2004. Effect of planting dates, nitrogen levels and bio-fertilization treatments on 1: Growth attributes of sugar beet (Beta Vulgaris L.). Basic Applied Science. 5, 227-237.
Kanimozhi, K., Panneerselvam, A., 2010. Studies on isolation and nitrogen fixation ability of Azospirillum spp. isolated from Thanjavur district. Der Chemica Sinica. 1, 138-145.
Kauseri, R.H., Athar, U.R., Ashraf, M., 2006. Chlorophyll fluoresce: A Potential indicator for rapid assessment of water stress tolerance in Canola. Pakistan Journal of Botany. 38, 1501-1509.
Khalili-Rad, R., Mirseyed Hosseini, H., 2016. Assessing some root morphological properties and efficiency indexes in several phosphorus efficient and inefficient cultivars of wheat. Journal of Soil Management and Sustainable Production. 6(3), 65-82 [In Persian with English Summary].
Khosravi, H., Mahmoudi, H., 2013. Evaluation of effects of Azotobacter inoculation and manure on growth of rainfed wheat. Journal of Soil Management and Sustainable Production. 3, 205-219 [In Persian with English Summary].
Leylasi Marand, M., Sarikhani, M., 2018. Investigation of Nitrogen Fixation Efficiency of Some Azotobacter Isolates by Maize Inoculation. Journal Agricultural Science and Sustainable Production. 27, 51-64. [In Persian with English Summary].
Mac Adam, J.W., Volenec, J.J., Nelson, C.J., 1989. Effects of nitrogen on mesophyll cell division and epidermal cell elongation intall fescue leaf blades. Plant Physiology. 89, 549-556.
Maghsoudi, E., Ghalavand, A., Aghaalikhani, M., 2014. Effect Management Strategies Fertilizer Nitrogen and Biological on Morphological Traits, Yield and Quality Traits Corn (S.C. 704). Iranian Journal of Field Crops Research. 12(2), 273-282. [In Persian with English Summary].
Malakouti, M.J., Nafisi, M., 2004. Fertilization of Dryland and Irrigated Soil. Tarbiat Modares University. 342p. [In Persian].
Manske, G.B., Luttger, A., Behl, R.K., VleK, P.G., Cimmit, M., 2000. Enhancement of mycorrhiza (VAM) infection, Nutrient efficiency and plant growh by Azotobacter chroococcum in wheat. In: Narula, N. (ed.), Azotobacter in Sustainable Agriculture. CBS Publishers & Distributors, New Dehli, India.
Markarian, Sh., Najafi, N., Aliasgharzad, N., Oustan, Sh., 2015. Effects of Sinorhizobium meliloti bacterium and phosphorus on leaf chlorophyll index, nitrogen and phosphorus concentrations in alfalfa shoot and root under drought stress conditions. Water and Soil Science. 25, 27-45. [In Persian with English Summary].
Mc Donald, G.K., 2002. Effects of nitrogen fertilizer on the growth grain yield and grain protein concentration of wheat. Australian Journal of Agricultural Research. 43, 949-967.
Mihalovic, N., Lazarevic, M., 1977. Chlorophyllaz activity in wheat (Triticum aestivum L.) leaves during drought and its dependence on the nitrogen on applied. Field Crops Research. 9, 46-58.
Mirahmadi, M., Malakuti, M.J., Khavazi, K., 2011. Effect of PSB on P uptake by corn in alkaline soils. 12th Iranian Soil Science Congress. University of Tabriz. Tabriz, Iran. [In Persian].
Mohtadi, M., Mirhadi, M.J., Chraty, A., Bahadori, M., 2015. Effects of biofertilizer containing of free living nitrogen fixing bacteria and phosphate solubilizing bacteria (PSB) on quantitative and qualitative traits of wheat. Soil Management and Sustainablen Production. 5, 229-242. [In Persian with English Summary].
Mooleki, S.P., Schoenau, J.J., Charles, J.L., Gwen, G., 2004. Effect of rat, frequency and incorporation of feedlot cattele manure on soil nitrogen availability crop performance and nitrogen use efficiency in east-central Saskachwan. Canadian Journal of Soil Science. 84, 199-210.
Naderikharaji, R., pakniyat, H., Biabani, A.R., 2008. Effect of Drought strees on photosynthetic Rate of Four Rapeseed (Brassica napus) Cultivars. Journal of Applied Sciences. 23, 4460-4463.
Nanda, S.S., Swain, K.C., Panda, S.C., Mohanty, A.K., Alim, M.A., 1995. Effect of nitrogen and biofertilizers in fodder rainfed upland conditions of Orisa. Current Agriculture Research. 8, 45-47.
Naserian, B., Asadi, A.A., Rahimi, M., Ardakani, M.R., 2007. Evaluation of wheat cultivars and mutants for morphological and yield traits and comparing of yield components under irrigated and rain fed conditions. Asian Journal of Plant Science. 6, 214-224.
Nikmehr, S., Akhgar, A., 2015. Effect of combined application of phosphate solubilizing bacteria and phosphrous fertilizer on growth and yield of sesame. Journal of Water and Soil. 29 (4), 991-1003. [In Persian with English Summary].
Pakdel, M., Maleki, A., Noormohammadi, GH., Fazel, SH., 2011. The effect of azotobacter and pseudomonas growth stimulating bacteria on yield and yield components of bread wheat under normal conditions and Drought Stress. Journal of Agronomy Research. 3, 107-121 [In Persian with English Summary].
Pandy, P.K., Maranville, J.W., Admou, A., 2001. Tropical wheat response to irrigation and nitrogen in a Sahelian environment. I. Grain yield, yield components and water use efficiency. European Journal of Agronomy. 15, 93-105.
Parentoni, S.N., de Souza Júnior, C.L., 2008. Phosphorus acquisition and internal utilization efficiency in tropical maize genotypes. Pesquisa. Pesquisa Agropecuaria Brasileira. 43, 893-901.
Patriquin, D.G., Dobereiner, J., 1978. Light microscopy observation of tetrazolium reducing in the endorhizosphere of maize and other grasses in Brazil. Canadian Journal of Microbioly. 24, 734-742.
Rafiq, M., Hussain, A., Ahmad, A., Basra, M.A., Wajid, A., Anwar, J., Ibrahim, M., Goheer, M.A., 2005. Effect of irrigation on agronomic traits of wheat (Triticum aestivum L.). International Journal of Biology and Biotechnology. 2, 751-759.
Sabir, S., Bakht, J., Shafi, M., Alishah, M., 2002. Effect of foliar application of different does nitrogen on wheat. Asian Journal of Plant Science and Research. 4, 300-303.
Sadat, A., 2007. Effects of vesicular arbuscular mycorhiza and plant growth promoting bacteria on nutrient uptake and yield of wheat under salinity condition. M.Sc. thesis. University of Tehran. [In Persian].
Sadeghi, S., Heidari, Gh., Sohrabi, Y., 2015. Effect of Biological Fertilizer and Fertilization Management on Some Growth Indices of Two Maize Varieties. Journal of Agricultural Science and Sustainable Production. 25, 43-60. [In Persian with English Summary].
Sani, B., Faezeh, R.Z., yaghati, H.L., ghoshchi, F., Karver, M., 2007. The role of biological fertilizers on the qualitative and quantitative indicators in corn crop ecosystem. Proceedings of the National Conference of Ecological Agriculture in Iran. 885-899. [In Persian].
Seyed Sharifi, R., Lotfollah, F., Kamari, H., 2016. Evaluation of effects of Azotobacter, Azospirillum and Psedomunas inoculation and spraying of nitrogen on fertilizer use efficiency and growth of Triticale. Journal of Soil Management and Sustainable Production. 5, 115-132. [In Persian with English Summary].
Shaharroona, B., Arshad, M., Zahir, Z.A., Khalid, A., 2006. Performance of Pseudomonas spp. Containing ACC-Deaminase for improving growth and yield of maize (Zea mays L.) in the presence of nitrogenous fertilizer. Soil Biology and Biochemistry. 38, 2971-2975.
Shen, J., Yuan, L., Zhang, J., Li, H., Bai, Zh., Chen, X., Zhang, W., Zhang, F., 2011. Phosphorus dynamics: From soil to plant. Plant Physiology. 156, 997-1005.
Singh, R., Behl, R.K., Singh, K.P., Jain, P., Narula, N., 2004. Performance and gene effects for wheat yield under inoculation of arbuscular mycorrhiza fungi and Azotobacter chroococcum. Haryana Agricultural University. Hisar, India. Plant, Soil and Enviroment. 50, 409-415.
Turan, M., Gullucea, M., Cakmakcie, R., Oztasa, T., 2010. The effect of PGPR strain on wheat, yield and quality parameters. In Proceedings of the World Congress of Soil Science, Soil19 Solutions for a Changing World 1 – 6 August Brisbane, Australia. 140-143.
Wu, S.C., Cao, Z.H., Chenng, K.C., Wong, M.H., 2005. Effect of biofertilizers containing N-fixer, P and K solubizers and AM fungi on maize growth: a greenhouse trial. Geoderma. 125, 155-166.
Zabihi, H.R., Savagebi, G., Khavazi, K., Ganjali, A., 2009. Response of wheat growth and yield to application of plant growth promoting rhizobacteria at various levels of phosphorus fertilization. Journal of Agronomy Research. 7, 41-51. [In Persian with English Summary].
Zahir, A.Z., Arshad, M., Frankenberger, W.F., 2004. Plant growth promoting rhizobacteria: applications and perspectives in agriculture. Journal Agronomy Research. 81, 97-168.
Zaidi, A., Mohammad, S., 2006. Co-inoculation effects of phosphate solubilizing microorganisms and glomus fasciculatum on green gram-bradyrhizobium symbiosis. Agriculture Science. 30, 223-230.
Zaied, K.A., Abd El-Hady, A.H., Afify Aida, H., and Nassef, M.A. 2003. Yield and nitrogen assimilation of winter wheat inoculated with new recombinant inoculants of rhizobacteria. Pakistan Journal of Biological Sciences. 6, 344-358.
Zemrany, H.El., Cortet, J., Lutz, M.P., Chabert, A., Baudoin, E.K., Haurat, J., Maughan, N., Fe´ Lixf, D., De´ fago, G., Bally, R., Moe¨ Nne-Loccoz, Y., 2006. Field survival of the phytostimulator Azospirillum lipoferum CRT1 and functional impact on maize crop, biodegradation of crop residues, and soil faunal indicators in a context of decreasing nitrogen fertilization. Soil Biology and Biochemistry. 38, 1712–1726.