Genetic variation and effects of salinity stress on a number of physiological characteristics in bread wheat mutant lines

Rahmani-Kamrodi, Atefeh; Navabpour, Saeid; Yamchi, Ahad; Nasrolahnejad-Ghomi, Ali Asghar

doi:10.22077/escs.2019.2047.1507

Document Type : Original Article

Authors

¹ Postgraduate Plant Breeding and Biotechnology Department, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

² Associate Professor, Plant Breeding and Biotechnology Department, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

³ Assistant Professor, Plant Breeding and Biotechnology Department, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

https://doi.org/10.22077/escs.2019.2047.1507

Abstract

The purpose of this experiment was study on genetic variation of wheat mutant lines (T-67-60 and T-65-7-1) and its parent (Tabasi cultivar) using RAPD molecular marker and also compare some biochemical parameters of salinity tolerance between the mutant lines and the parent in response to salt stress in the seedling stage.In this study, two mutant lines of wheat (T-67-60 and T-65-7-1) with its parent (Tabasi cultivar), cultured in the Yoshida hydroponics at 30 °C and lighting condition (16 hours of light). Then salinity stress induced on wheat seedling at electrical conductivity 6 dS m^-1 and leaf samples were collected for DNA extraction and evaluation of biochemical parameters (proline, protein and chlorophyll content).The pattern of RAPD marker showed more similarity of line mutant T-67-60 with parent (Tabasi cultivar).The results of biochemical parameters showed that mutant lines (T-67-60 and T-65-7-1) were higher tolerance than parent (Tabasi cultivar) under salinity stress.Variability of mutations induced by gamma radiation in wheat revealed and verified with molecular RAPD primers. In addition, differences in biochemical characteristics under salinity condition in the mutant lines (T-67-60 and T-65-7-1) and the parent (Tabasi) implies genetic variation for these traits.

Keywords

References

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Environmental Stresses in Crop Sciences

Genetic variation and effects of salinity stress on a number of physiological characteristics in bread wheat mutant lines

References

References

Volume 13, Issue 2
Open Access Policy
July 2020
Pages 613-623

Genetic variation and effects of salinity stress on a number of physiological characteristics in bread wheat mutant lines

References

References

Volume 13, Issue 2 Open Access PolicyJuly 2020Pages 613-623

Volume 13, Issue 2
Open Access Policy
July 2020
Pages 613-623