Environmental Stresses in Crop Sciences

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Effects of salicylic acid and cycocel on percentage and yield of essential oil and physiological characteristics on Moldavian balm (Dracocephalum moldavica L.) under drought stress conditions

Document Type : Original Article

Authors

1 Ph.D. Agriculture and Plant Breeding, Faculty of Agriculture, Zanjan University

2 Assistant Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Iran

3 Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Iran

4 Research Division of Natural Resources, Zanjan Agricultural and Natural Resources Research and Education Centre, AREEO, Zanjan, Iran

Abstract

Introduction
Drought is one of the most important environmental stresses that affects the morphology, physiology and biochemistry of the plant and it has a major impact on agricultural production. The Moldavian balm is herbaceous that originated in middle Asia and about 66 compound are identified in this plant. Application of growth regulator lead to increases of plant resistance against biotic and abiotic stress and is involved as a strategy to prevents adverse effects of environmental stress. According to semi-sensitivity of dragonhead plant to drought condition, its medicinal importance as well as, its industrial widespread use, this study was conducted to evaluate dragonhead essential oil yield and its compound under drought condition and also, to evaluate physiological aspect of drought resistance.
Material and methods
In order to investigate the effects of salicylic acid and cycocel on the gas exchange on Moldavian balm in drought stress condition research was conducted in the spring of 2014 and 2015 at research farm of agricultural college, Zanjan University. The experiment was laid out in split plot with two factors of moisture levels and growth regulators in a randomized complete block design with four replications. The main factor is the moisture levels at two levels without stress (Complete irrigation until the end of the growth period), drought stress (Complete irrigation before start flowering and discontinuation of irrigation until the end of the growth period). Sub-substrate of different levels of salicylic acid (800 and 1600 μm), cycocel (600 and 1200 μm), and control (dissolve with distilled water), which applied for a single application before start flowering.

Findings
The results of two-year combined analysis of variance showed that the interactions of the sampling stage in the year in moisture for leaf relative water content, canopy temperature, essential oil percent, essential oil yield, plant fresh weight and plant dry weight was significant at the 1% probability level. Mean interactions of growth regulator in during sampling stage showed the salicylic acid treatment (800 μM) at the end of flowering stage (H3B1) had the highest percentage of essential oil (0.37) and the lowest percentage of essential oil is related to the 600 micro molar (0.16) cycocel treatment at the flowering stage (H1B2).

Conclusion 
The percentage of essential oil in the start flowering stages, flowering and flowering ends is increasing, so that the highest percentage of essential oil is associated with the flowering ends stage. Under stress conditions, the essential oil yield is reduced. Cycocel treatments 1200 micro molar it was caused by increased root growth and increased water absorption from the depths of the soil the plant has more essential oil than other treatments. In drought stress conditions, essential oil yield decreased and essential oil percentage increased. Also, by applying drought stress, the water status of the plant changes significantly and the relative water content of the leaves decreases. According to the results of the experiment, it can be concluded that drought stress reduced the yield and essential oil percentage of the Moldavian balm.The use of salicylic acid in conditions of drought stress compared to cycocel increases the yield and essential oil percentage.

Keywords

References
Abbaspor, H., Rezaei. H., 2014. Effects of salicylic acid and jamonic acid on hill reaction and photosynthetic pigment (Dracocephalum moldavica L.) in different levels of drought stress. International Journal of Advanced Biological and Biomedical Research. 2, 2850-2859.
Aflatuni, A., 2005. Water stress effect on growth and yield of (Coriandrum sativum L.) and its alleviation by n-triacontanol. Acta Horticulture. 79, 19-24.
Aliabadi-Farahani, H., Valadabadi, S.A., Daneshian, J., Khalvati, M.A., 2009. Evaluation changing of essential oil of balm (Melissa officinalis L.) under water deficit stress conditions. Journal of Medicinal Plant Research. 3, 329-333.
Amiri, A., Ismail Zadeh Bahabadi, P., Yidulahi ten Cheshmeh, P., Sirus Mehr, AS.R., 2017. The role of soluble salicylic acid and chitosan in water stress conditions on some physiological traits and yield of safflower oil (Carthamus tinctorius L.). Ecophysiology of Crops. 1, 84-69. [In Persian with English summary].
Andalibi, B., 2009. Effect of Anetum graveolens on growth and development during limited irrigation. Ph.D. Thesis. Field of physiology of crops. School of Agriculture. Tabriz University. [In Persian].
Andlibi, B., Nori. F., 2014. The effect of cycocel on photosynthetic activity and essential oil of Foeniculum vulgare under drought stress. Plant Biology of Iran, 29, 91-104. [In Persian with English summary].
Babaei B., 2011. Effect of cycocel on quantitative and qualitative characteristics of Ocimum bosilicus L. under drought stress. MSc dissertation, Faculty of Agriculture, the University of Zabol, Iran. [In Persian].
Baher, Z. F., Mirza, M., Ghorbanli, M., Rezaii, M.B., 2002. The influence of water stress on plant height, herbal and essential oil yield and composition in Saturja hortensis L. Flavor and Fragrance Journal. 17, 275-277.
Baher, Z., Rezaei, M., Ghorbanli, M., Asgari, F. Iraqi, MK. 2004. Evaluation of metabolic changes due to drought stress in Sorghum. Investigating Iranian Medicinal and Aromatic Herbs. 20, 275-263. [In Persian with English summary].
Bahrenejad, B., Razmjoo, J., Mirza, M. 2013. Influence of water stress on morph physiological and phytochemical traits in Thymus daenensis. International Journal of Plant Production.7:155-166.
Cutt, J.R., Klessig D.F., 1992. Salicylic acid in plants. A changing perspective. Pharmaceutical Technology. 16, 25-34.
Dunford, N.T., Vazquez, R.S. 2005. Effect of water stress on plant growth and thymol and carvacol concentrations in Mexican oregano grown under controlled conditions. Journal of Applied Horticulture. 7, 20-22.
Eman, E., Aziz, S.T., Hendawi, Ezz El Din, A., Omer, E.A., 2008. Effect of soil type and irrigation intervals on plant growth, essential oil yield and constituents of Thymus vulgaris plant. American-Eurasian Journal of Agricultural and Environmental Science. 4, 443-450.
Heidari, N., Pouryousef, N., Tavakkoli, A., Saba, J., 2012. Effect of drought stress and harvesting date on yield and essential oil production of anise (Pimpinella anisum L.). Iranian Journal of Medicinal and Aromatic Plants. 28, 121-130. [In Persian with English summary].
Hassan zadeh, K., Hemmati, Kh., Alizadeh, M., 2016. Effect of Organic and Salicylic Acid on Performance and Some Secondary Metabolites of Melissa officinalis L. Plant Breeding Research. 20, 107-130.
Hussein, M.S., El-Sherbeny, S.E., Khalil, M.Y., Naguib, N.Y., Aly, S.M., 2006. Growth characters and chemical constituents of Dracocephalum moldavica L. plants in relation to compost fertilizer and planting distance. Scientia Horticulturae. 108, 322-331.
Jami, N., Mousavi Nik, S.M., Taghizadeh, M., 2015. The effect of drought stress and foliar application with salicylic acid on qualitative and quantitative yield of Black cumin under Kerman climatic conditions. Journal of Crops Improvement. 17(3), 827-840. [In Persian with English summary].
Khandelwal S.K., Guptanarendra K., Sahu M.P., 2002. Effect of plant growth regulators on growth, yield and essential oil production of henna (Lawsonia inermis L.). Horticultural Science and Biotechnology. 5, 71-77
Kordi, S., Saidi, M., Ghanbari, F., 2013. Induction of drought tolerance in sweet basil (Ocimum basilicum L.) by salicylic acid. International Journal of Agricultural and Food Research. 2, 18-26.
ZMayek-Perez, N., Garcia-Espinosa, R., Lopez-Castaneda, C., Acosta-Gallegos, J., A., & Simpson, J., 2002. Water relations, histopathology and growth of common bean (Phaseolus vulgaris L.) during pathogenesis of Macrophomina phaseolina under drought stress. Physiological and Molecular Plant Pathology. 60, 185-195.
Merah, O., 2001. Potential importance of water status traits for durum wheat improvement under Mediterranean conditions. Journal of Agricultural Research. 137, 139-145.
Mohamed, B.R., El-Sayad, A.A., Fawzi, A.F. A., 1983. Effect of gibberellic acid and chlormequat on yield and oil quality of geranium (Pelargonium graveolens). Acta Horticulturae. 132, 265-272.
Mohammadi Babazidi, H., Falkanaz, M., Heydari, P., Hemati, M., Farokhian, Sh., 2013. Effect of azospirilium and salicylic acid on physiological and morphological traits of basil (Ocimum basilicum L.) under water stress. Journal of Cellular-Molecular Biotechnology 3, 31-36
Mohammadpour, M. Negahban, M., Saeedfar, S., Salehi Shanjan, P., Javadi, H., 2015. Effect of drought stress on some of the biochemical characteristics of three Achillea population (Achillea vermicularis). Russian Journal of Biological Research. 4, 68-80.
Mohtashemi, M., Puriusf, M., Andalibi, B., Bakhtiari, F., 2015. Effect of spraying and pretreatment with salicylic acid on yield and essential oils of fennel (Feniculum vulgare L.) under drought stress conditions. Biomedical Journal of Iranian Herbs and Medicinal Herbs Research. 31,841-852.
Mozhafrian, V., 2003. Culture of the Iranian Plant Names. Contemporary Culture Publications. Tehran. 362p. [In Persian].
Naderi, N., Haha, H., Ahmadi, H., 2015. The effect of salicylic acid on some physiological characteristics of coriander (Coriandurum sativum L.). Scientific Journal of Ecophysiology of Agricultural Plants. 7(4), 287-305. [In Persian with English summary].
Nurhan, T.D., Vazquez, R.S., 2005. Effect of water stress on plant growth and thymol and carvacrol concentration in Mexican oregano grown under controlled conditions. Journal of Applied Horticulture. 7, 20-22.
O’Neill, P.M., Shanahan, J.F., Schepers, J.S., 2006. Use of chlorophyll fluorescence assessments to differentiate corn hybrid response to variable water conditions. Crop Science. 46, 681-687.
Pastenes, C., Pimentel, P., Lillo, J., 2005. Leaf movements and photo inhibition in relation to water stress in field-grown beans. Experimental Botany. 56, 425-433.
Pinter, P.J., Zipoli, G., Reginato, R.J., Jackson, R.D., Idso, S.B., Homan, J.P. 1990. Canopy temperature as an indicator of differential water use and yield performance among wheat cultivars. Agricultural Water Management. 18, 35-48.
Pirzad, A., Alyari, H., Shakiba, M.R., ZehtabSalmasi, S., Mohammadi, A., 2006. Essential oil content and composition of German chamomile (Matriciria chamomilla L.) at different irrigation regimes. Agronomy Journal. 5, 451-455.
Raghavan, B., Abraham, K.O., Shankaranarayan, M.L., Koller, W.D., 1994. Studies on flavor changes during drying of dill (Anethum sowa Roxb) leaves. Journal of Food Quality. 17,457-466.
Rahbarian, P., Salehi Sardoei A., 2014. Effects of drought stress and manure on dray herb yield and essential oil of dragonhead (Dracocephalum moldavica) in Jiroft area. International Journal of Bioscience. 9, 212-217.
Ramrudi, M., Khoram, AS., 2013. Interaction effects of salicylic acid spraying and irrigation treatments on some quantitative, qualitative and osmotic factors of Basil. Journal of Applied Ecophysiology of Plants. 1, 19-32. [In Persian with English summary].
Rashid, A., Stark, J.C., Tanveer, A., Mustefa, T., 1999. Use of canopy temperature measurement as a screening tool for drought tolerance in spring wheat. Journal of Agronomy and Crop Science. 182, 231-237.
Raskin, I. 1992. Role of salicylic acid in plants. Annual Review of Plant Physiology and Plant Molecular Biology. 43, 439-463.
Rezaei Chineh, A. 2012. Effect of different irrigation treatments on the accumulation of essential oils, and some Ecophysiological traits in fennel (Foeniculum vulgare L.). Ph.D. Thesis. Department of Agriculture, Faculty of Agriculture, Tabriz University. [In Persian].
Rezaei Chineh, A. and Pirzad A., 2014. Effect of salicylic acid on yield, yield components and essential oil of Nigella sativa L. under low water stress conditions. Iranian Crop Research, 12(3), 437-427. [In Persian with English summary].
Safikhani, F., Heydarye Sharifabadi, H., Syadat, A., Sharifi Ashorabadi, A., Syednedjad M., Abbazadeh, B., 2007. The effect of drought on yield and morphologic characteristics of Deracoceohalum moldavica. Iranian Journal of Medicinal and Aromatic Plants. 23, 183- 194. [In Persian with English summary].
Saharkhiz, M.J., Omidbaigi, R., Sefidkon, F., 2007. The effect of different levels of phosphorus and irrigation on secondary metabolites of medicinal plant Matricaria chamomile. Iranian of Medicinal Plant Third Conference, Shahed University, 2-3 October.
Salarpur Ghraba, F., Frahbakhsh, H., 2016. Effect of salicylic acid on some physiological traits, functional functions of fennel (Foeniculum vulgare L.) under drought stress. Biomedical Journal of Iranian Herbs and Medicinal Herbs Research. 32(2), 216-230. [In Persian with English summary].
Sarmadnia, G., Koocheki, A., 1995. Importance of Environmental Stresses in Agronomy. Mashhad Jahad Daneshgahi Publications. [In Persian].
Shakari, F., Pak Mehr, A., Truth, M., Saba, J., Tasks, M. And Zangani, A. 2010. The effect of salicylic acid priming on some morphological traits Cowpea (Vigna unguiculata L.) under drought stress condition in potting stage. Modern Technologies in Agriculture. 4, 5-26. [In Persian with English summary].
Sibi, M., 2011. Effect of water stress, zeolite and foliar application of salicylic acid on some of agronomical and physiological traits in spring safflower. M.Sc. Thesis. Agronomy and Crop Development Department. College of agriculture. Islamic Azad University, Branch, Arak. 213. [In Persian].
Siddique, M.R.B., Hmid, A., Islam, M.S., 2000. Drought stress effects on water relations of wheat. Botanica Academic Sinica. 41, 35-39.
Singh, B., Usha, K., 2003. Salicylic acid induced physiological and biochemical changes in wheat seedlings under water stress. Plant Growth Regulation. 30, 137-141.
Soroori, S., Moghadam, M., Hasheme moghadam, H., 2013. Effect of nitrogen fertilizer and row spacing on yield and amount of essential oil Dracocephalum moldavica L. Journal of Agricultural Improvement. 4, 179-194.
Venskutionis, P.R., Dapkevicius, A., Baranuauskiene, M., 1995. Flavour composition of some lemon-like aroma herbs from Lithuania. Development in Food Science. 37, 833-847.
Wanjura, D.F., Maas, S.J., Winslow, J.C., Upchurch, D.R., 2004. Scanned and sport measured canopy temperature of cotton and corn. Computers and Electronics in Agriculture. 44, 33-48.
Yazdani, D., Shahnazi, S., Seifi, H., 2004. Cultivation of Medicinal Plants. Vol. 1. Shahid Beheshti Jahad University Press. . [In Persian].
Yousefzadeh, S., Modares Sanavi, S.A., Sefidkan, F., Asgharzadeh, A., Ghalavand, A., 2010. Effect of different stages of harvesting on the amount and composition of essential oils in Moldavian balm (Dracocephalum moldavica L.). Journal of Iranian Medicinal and Aromatic Plants. 21, 561-573.  . [In Persian with English summary].
Zho, H., Tan, J., Qi, C., 2009. Photosynthesis of Rehmannia glutinosa subjected to drought stress in enhanced by choline chloride through alleviating lipid peroxidation and increasing proline accumulation. Plant Growth Regulation. 51, 255-262.
Environmental Stresses in Crop Sciences
Volume 12, Issue 3
Open Access Policy
October 2019
Pages 865-876
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History
  • Receive Date: 15 April 2018
  • Revise Date: 29 May 2018
  • Accept Date: 30 May 2018
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