تأثیر محلول‌پاشی اسید آسکوربیک و سدیم نیتروپروساید بر عملکرد دانه، روغن و برخی صفات زراعی گلرنگ بهاره (Carthamus tinctorius L.) در شرایط تنش کم آبیاری

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد زراعت دانشگاه صنعتی شاهرود

2 عضو هیات علمی دانشکده کشاورزی دانشگاه صنعتی شاهرود

چکیده

به‌منظور ارزیابی تأثیر اسید آسکوربیک و سدیم نیتروپروساید بر عملکرد دانه، روغن و برخی صفات گلرنگ (Carthamus tinctorius)، پژوهشی در سال 1390 در دانشگاه شاهرود انجام شد. تیمارهای آزمایش شامل 2 سطح آبیاری هر 8 روز یک‌بار (تیمار عدم تنش) و هر 16 روز یک‌بار (تیمار تنش) به‌عنوان فاکتور اصلی و 3 سطح اسید آسکوربیک (صفر، 10 و 20 میلی مولار) و 3 سطح سدیم نیتروپروساید (صفر، 50 و 100 میکرو مولار) به‌صورت محلول پاشی برگی به‌عنوان فاکتورهای فرعی بودند که در قالب آزمایش اسپلیت پلات فاکتوریل بر پایه طرح بلوک­های کامل تصادفی در 3 تکرار قرار گرفتند. تیمار آبیاری بعد از استقرار کامل بوته‌ها اعمال گردید. در 63 و 65 روز پس از کاشت به ترتیب محلول‌پاشی با سدیم نیتروپروساید و اسید آسکوربیک انجام شد و یک هفته بعد تکرار گردید. نتایج نشان داد که تنش کم‌آبیاری موجب کاهش محتوای نسبی آب برگ، وزن مغز دانه و تعداد دانه در طبق شد. در مقابل نسبت پوسته به مغز دانه در شرایط تنش افزایش یافت. اگرچه در اثر محلول ­پاشی اسید آسکوربیک با غلظت پایین محتوای نسبی آب برگ کاهش نشان داد ولی در همین شرایط تعداد طبق در بوته به‌طور متوسط 2/5 طبق در بوته افزایش یافت. استفاده از سطح سوم سدیم نیتروپروساید (100 میکرو مولار) موجب افزایش معنی‌دار وزن هزار دانه، عملکرد دانه (13/2 درصد)، عملکرد روغن (17/2 درصد) و درصد روغن (4/6 درصد) گردید. لذا این­گونه استنباط می‌شود که کاربرد برگی سدیم نیتروپروساید با غلظت مناسب می‌تواند در کاهش شدت تنش کم‌آبی مفید باشد.

کلیدواژه‌ها


Abbasi Siahjani, E., 2008. Effect of drought stress on morphological and agronomical characteristics of sunflower. MSc. Thesis of Agronomy. Islamic Azad University of Tabriz. Iran. [In Persian with English summary].

Arasimowics, M., Wieczoorek, J.F., 2007. Nitric oxide as a bioactive signaling molecule in plant stress responses. Journal of Plant science. 172, 876- 887.

Ashraf, M., Foolad, M.R., 2007. Roles of glycine betaine in improving plant abiotic stress resistance. Journal of  Environmental and Experimental Botany. 59, 206-216.

Baghkhani, F. Farahbakhsh, H., 2008. Effects of drought stress on yield and some physiological characters of three spring safflower (Carthamus tinctorius) varieties. Journal of water. Soil and plant in Agricultural. 8(2), 45-57 . [In Persian].

Baji, M., Kinet. J.M., Lutts, S., 2001. The use of the electrolyte leakage method for assessing cell membrane stability as a water stress tolerance test in durum wheat. Journal of Plant Growth Regulation. 4(2): 1-10.

Behadani, M.A., Jamiolahmadi, M., 2009. Reaction of safflower in birjand to drought stress. Iranian Journal of Field Crops Research. 8(2), 315-323 . [In Persian].

Beligni, M.V., Lamatina, L. 1999. Nitric oxide protect against cellular damage produced by methyl viologen herbicides in potato plants. Nitric oxide. Journal of Biological Chemistry. 3, 199-208.

Colom, M.R., Vazzana, C. 2003. Photosynthesis and PSII functionality of drought resistant and drought- sensitive weeping lovegrass plants, Journal of Environmental and Experimental Botany. 49, 135- 144.

Del Rio, L.A., Corpas, F.J. Barroso, J.B., 2004. Nitric oxide and nitric oxide synthase activity in plants. Journal of Phytochemistry. 65, 783-792.

Dolatabadian, A., Modares Sanavy, A.M. Asilan, K. 2009. Effect of ascorbic acid foliar application on yield, yield component and several morphological traits of grain corn under water deficit stress conditions. Journal of  Notulae Scientia Biologicae. 2(3), 45-50. [In Persian with English summary].

Esfandiari, E.A., Shakiba, M.R., Mahboob, S.A., Alyari, H., Toorchi, M., 2007. Water stress, antioxidant enzyme activity and lipid peroxidation in wheat seedling. Journal. of Food, Agriculture and Environment. 5, 149-153.

Farrokhinia, M., Roshdi, M., Pasbaneslam, B., Sasandoost, R., 2011. Study of some physiological traits and yield in spring safflower under water deficit stress. Iranian Journal of Field Crops Science. 42(3), 545- 553. [In Persian with English summary].

Farooq, M., Basra, S.M.A. Wahid, A., Rehman, H., 2009. Exogenously applied nitric oxide enhances the drought tolerance in fine grain aromatic rice (Oryza sativa L.). Journal Agronomy and Crop Science. 195, 254-261.

FechtChristoffers, M.M., Maier, P., Horst, W.J.,  2003. Apoplastic peroxidases and ascorbate are involved in manganese toxicity and tolerance of Vigna unguiculata. Journal of Plant Physiology. 117, 237- 244.

Garratt, L.C., Janagoudr, B.S., Lowe, K.C., Anthony, P., Power, J.B., Davey, M.R.,  2002. Salinity tolerance and antioxidant status in cotton cultures. Free Radical Biology and Medicine. 33(4), 502-511.

Gracia Mata, C., Lamattina, L., 2001. Nitric oxide induces stomatal closure and enhances the adaptative plant responses against drought stress. Journal of Plant Physiology. 126, 1196-1204.

Hamrouni, I., Ben Salah, H., Marzouk, B., 2001. Effect of water deficit on lipids of safflower aerial parts. Journal  of Photochemistry. 58, 277-280.

Kafi, M., Rostami, M., 2008. Effect of drought stress in reproductive growth stage on yield and yield  components and oil content of three safflower cultivars in irrigation with salty water conditions. Journal of Agronomy Research. 5(1), 121-131. [In Persian with English summary].

Khajehpoor, M., 2007. Industrial Plant. Publications of Esfahan University of technology. 580p. [In Persian].

Knowles, P.F., 1999. Safflower. In: Roebbelen, G. (ed.), Oil Crops of the World. Mc Graw Hill Book Company. pp: 363-373.

Koocheki, A., Sarmadnia, G.H., 2003. Crop Physiology. Jahad-e-Daneshgahi Mashhad Press. pp. 400. [In Persian].

Kumar, H., 2000. Development potential of safflower in comparision to sunflower. Sesame and safflower news letter. Annals of Botany.15, 86-89.

Kumari, A., Sheokand, A., Kumari, S. 2010. Nitric oxide induced alleviation of toxic effects of short term and long term Cd stress on growth, oxidative metabolism and Cd accumulation in Chickpea. Brazilian society of Plant physiology. 22, 271-284.

Laspina, N.V., Groppa, M.D., Tomaro, M.L. Benavides, M.P., 2005. Nitric oxide protects sunflower leaves against Cd induced oxidative stress. Plant Science. 169, 323-330.

Lei, Y., Yin, C., Ren, J., Li, C., 2007. Effect of osmotic stress and sodium nitroprusside pretreatment on proline metabolism of wheat seedlings. Biologia Plantarum Journal. 51, 386-390.

Misra, A.N., Biswal, A.K., Misra, M., 2002. Physiological, biochemical and molecular aspects of water stress responses in plants and the biotechnological application. Proceedings of the National Academy Science. 2, 115-134.

Naderi Darbaghshahi, M.R., Noormohammadi, G., Majidi, A., Darvish, F., Shirani Rad, A.H., 2004. Response of three safflower lines in different drought stress levels. Journal of Agricultural Science. 10(4), 3-15. [In Persian with English Summary].

Nabavi kelat, M., Karimi, M., Noormahammadi, G., Sadr Abadi, R., Azizi, M., 2005. Determiniation of suitable planting date and plant population in autumn cultivation of safflowers in Jovain–Sabzevar.  Journal of Agricultural Science. 11(4): 145- 158. [In Persian with English summary].

Nasibi, F., Manoochehri Kalantari, K., Khodashenas M., 2009. The effect of sodium nitroprusside (SNP) pretreatment on some biochemical parameters in tomato seedlings (Lycopersicun esculentum) under drought stress. Journal of Agricultural Science Natural Resources. 16(2), 121-135. [In Persian with English Summary].

Neill, S., Barros, R., Bright, J., Desikan, R., Hancock, J., Harrisan, J., Morris, P., Ribeiro, D. Wilson, I., 2008. Nitric oxide, stomatal closure, and abiotic stress. Journal of Experimental Botany. 59, 165-176.

Omidi, A.H., 2008. Effect of drought stress at different growth stages on seed yield and some agro-physiological traits of three spring safflower cultivars. Seed and Plant Prouduction Journal. 2(1), 15-31. [In Persian with English Summary].

Omidi, F., Sepehri, A., 2013. Effect of Sodium Nitroprusside on growth, yield and yield components of bean (Phaseolus vulgaris L.) under water deficit stress. Iranian Journal of Field Crops Science. 45(2), 243-254. [In Persian with English Summary].

Shau, M.P., Solanki, N.S., Dashora, L.N., 1993. Effects of thiourea, Thiamine and ascorbic acid on growth and yield of maize. Journal of Agronomy and Crop Science. 171, 65-69.

Shalata, A., Neumann, P.M., 2001. Exogenous ascorbic acid increase resistance to salt stress and reduces lipid peroxidation. Journal of Exoerimental Botany. 52, 2207- 2211.

Sheokand, S., Kumari, A., Sawhney, V., 2008. Effect of nitric oxide and putrescine on antioxidative responses under NaCl stress in chickpea plants. Journal of Physiology and Molecular Biology of Plants. 14(4), 355-362.

Sheokand, S., Bhankar, V., Sawhney, V., 2010. Ameliorative effect of exogenous nitric oxide on oxidative metabolism in NaCl treated chickpea plants. Brazilian society of plant Physiology. 22, 81-90.

Soha, E., Nahed, G., Bedour, H., 2010. Effect of water stress, ascorbic acid and spraying time on some morphological and biological composition of Ocimum basilicum plant. Journal of American Science. 6(12), 33-44.

Tavakolee Zeniali, A., 2002. Study of irrigation cutting during growth stages on seed and oil yield and its components in safflower. M.Sc. dissertation, University of Tehran, Iran. [In Persian].

Tian, X., Li, Y., 2006. Nitric oxide treatment alleviates drought stress in wheat seedlings, Journal of plant Biology. 50, 775-778.