نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجو دکتری، گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران

2 دانشیار، گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران

3 استاد، گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران

چکیده

تنش شوری یکی از مهمترین تنش‌های غیرزیستی می‌باشد که عملکرد کلزا را تحت تاثیر قرار می-دهد. به منظور مطالعه برخی از پاسخ های فیزیولوژیکی کلزا و تغییرات بیان ژن BnaCDPK14 در شرایط تنش شوری، دو رقم متحمل (Slm046 و زرفام) و دو رقم حساس (اکاپی و ساریگل) در اتاقک رشد کشت شدند و تنش شوری از طریق آبیاری با آب حاوی 100 و 200 میلی‌مولار نمک (NaCl) اعمال شد. محتوای نسبی آب برگ، نشت یونی، آنزیم گایاکول پراکسیداز، آنزیم کاتالاز و بیان ژن پروتئین کیناز وابسته به کلسیم 14 کلزا اندازه‌گیری شدند. در شرایط شوری بالاتر (200 میلی مولار نمک کلرید سدیم) محتوای نسبی آب کاهش و نشت یونی افزایش یافت. فعالیت آنزیم گایاکول پراکسیداز، آنزیم کاتالاز و بیان ژن BnaCDPK14 نیز تحت تنش افزایش یافت که مقدار این افزایش در ارقام متحمل بیشتر از ارقام حساس بود. همبستگی بالای منفی بین صفات محتوای نسبی آب برگ و نشت یونی مشاهده شد. همبستگی بالا مثبت بین صفات گایاکول پراکسیداز، آنزیم کاتالاز و بیان ژن BnaCDPK14 وجود داشت که نشان می دهد با افزایش گونه‌های فعال اکسیژن، گیاه برای مقابله با آن سیستم آنتی اکسیدان خود را بکار می‌گیرد.

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