نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجو دکتری، گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران
2 دانشیار، گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران
3 استاد، گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران
چکیده
تنش شوری یکی از مهمترین تنش‌های غیرزیستی می‌باشد که عملکرد کلزا را تحت تاثیر قرار می-دهد. به منظور مطالعه برخی از پاسخ های فیزیولوژیکی کلزا و تغییرات بیان ژن BnaCDPK14 در شرایط تنش شوری، دو رقم متحمل (Slm046 و زرفام) و دو رقم حساس (اکاپی و ساریگل) در اتاقک رشد کشت شدند و تنش شوری از طریق آبیاری با آب حاوی 100 و 200 میلی‌مولار نمک (NaCl) اعمال شد. محتوای نسبی آب برگ، نشت یونی، آنزیم گایاکول پراکسیداز، آنزیم کاتالاز و بیان ژن پروتئین کیناز وابسته به کلسیم 14 کلزا اندازه‌گیری شدند. در شرایط شوری بالاتر (200 میلی مولار نمک کلرید سدیم) محتوای نسبی آب کاهش و نشت یونی افزایش یافت. فعالیت آنزیم گایاکول پراکسیداز، آنزیم کاتالاز و بیان ژن BnaCDPK14 نیز تحت تنش افزایش یافت که مقدار این افزایش در ارقام متحمل بیشتر از ارقام حساس بود. همبستگی بالای منفی بین صفات محتوای نسبی آب برگ و نشت یونی مشاهده شد. همبستگی بالا مثبت بین صفات گایاکول پراکسیداز، آنزیم کاتالاز و بیان ژن BnaCDPK14 وجود داشت که نشان می دهد با افزایش گونه‌های فعال اکسیژن، گیاه برای مقابله با آن سیستم آنتی اکسیدان خود را بکار می‌گیرد.
کلیدواژه‌ها
Abedi, T., Pakniyat, H., 2010. Antioxidant enzyme changes in response to drought stress in ten cultivars of oilseed rape (Brassica napus L.). Czech Journal of Genetics and Plant Breeding. 46(1), 27-34.
Aebi, H., 1984. Catalase in vitro. Methods in Enzymology. 105, 121-126.
Asada, K., 1992. Ascorbate peroxidase- a hydrogen peroxide scavenging in plant. Plant Physiology. 85, 235–241.
Asano, T., Hayashi, N., Kobayashi, M., Aoki, N., Miyao, A., Mitsuhara, I., Ichikawa, H., Komatsu, S., Hirochika, H., Kikuchi, S., Ohsugi, R., 2012. A rice calcium-dependent protein kinase OsCPK12 oppositely modulates salt-stress tolerance and blast disease resistance. The Plant Journal. 69(1), 26–36.
Asano. T., Hakata. M., Nakamura, H., Aoki, N., Komatsu, S., Ichikawa, H., Hirochika, H., Ohsugi, R., 2011. Functional characterisation of OsCPK21, a calcium-dependent protein kinase that confers salt tolerance in rice. Plant Molecular Biology. 75, 179–191.
Ashraf, M., 2004. Salinity tolerance in Brassica Oilseeds. Critical Reviews in Plant Sciences. 23(2), 157–174.
Ashraf, M., Ali, Q., 2008. Relative membrane permeability and activities of some antioxidant enzymes as the key determinants of salt tolerance in canola (Brassica napus L.). Environmental and Experimental Botany. 63, 266-273.
Besma, B.D., Denden, M., 2012. Effect of salt stress on growth, anthocyanins, membrane permeability and chlorophyll fluorescence of okra (Abelmoschus esculentus L.) seedlings. American Journal of Plant Physiology. 7, 174- 183.
Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry. 72, 248-254.
Bybordi, A., Tabatabaei, J., 2009. Effect of salinity stress on germination and seedling properties in canola cultivars (Brassica napus L). Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 37(1), 71- 76.
Chinnusamy, V., Jagendorf, A., Zhu, J.K., 2005. Understanding and improving salt tolerance in plants. Crop Science. 45, 437- 448.
Chrysargyris, A., Michailidi, E., Tzortzakis, N., 2018. Physiological and biochemical responses of Lavandula angustifolia to salinity under mineral foliar application. Frontiers in Plant Science. 9, 489.
Cicek, N., Cakirlar, H., 2002. The effect of salinity on some physiological parameters in two maize cultivars. Bulgarian Journal of Plant Physiology. 28, 66-74.
Dionisio-Sese, M.L. and S. Tobita, 1998. Antioxidant responses of rice seedlings to salinity stress. Plant Science. 135, 1-9.
FAO, 2012. FAO Statistical Year Book 2012, World Food and Agriculture. Food and Agriculture Organization of the United Nation, Rome, p. 366. http://www. fao.org/docrep/015/i2490e/i2490e00.htm.
FAO, 2017. FAOSTAT, Available online at: http://www.fao.org/faostat/en/#data/QC. Rapeseed production, 2014; Crops/Regions/World list/Production Quantity (pick lists) (Accessed December 22, 2017).
Farkhonded, R., Nabizadeh, E., Jalilnezhad, N., 2012. Effect of salinity stress on proline content, membrane stability and water relation in two sugar beet cultivars. International Journal of Agricultural Science. 2(5), 385-392.
Hasegawa, P.M., Bressan, R.A., Zhu, J.K., Bohnert, H.J., 2000. Plant cellular and molecular responses to high salinity. Annual Review Plant Physiology and Plant Molecular Biology. 51, 463–499.
Hepler, P.K., 2005. Calcium: a central regulator of plant growth and development. Plant and Cell. 17, 2142–2155.
Hniličková1, H., Hnilička, F., Orsák, M., Hejnák, V., 2019. Effect of salt stress on growth, electrolyte leakage, Na+ and K+ content in selected plant species. Plant, Soil and Environment. 65 (2), 90–96.
Homaee, M., Feddes, R.A., Dirksen, C., 2002. A macroscopic water extraction model for non-uniform transient salinity and water stress. American Journal of Soil Science Society. 66, 1764-1772.
Jiang, X., Qi, W., Xu, X., Li, Y., Liao, Y., Wang, B., 2014. Higher soil salinity causes more physiological stress in female of Populus cathayana cuttings. Acta Ecologica Sinica. 34(4), 225–231.
Kaya, C., Higges, D., Kirnak, H., 2001. The effects of high salinity (NaCl) and supplementary phosphorus and potassium on physiology and nutrition development of spinach. Bulgican. Journal of Plant Physiology. 27, 47-59.
Kukreja, S., Nandwal, A.S., Kumar, N., Sharma, S.K., Unvi, V., 2005. Plant water status, H2O2 scavenging enzymes, ethylene evolution and membrane integrity of Cicer arietinum roots as affected by salinity. Biologia Plantarum. 49(2), 305–308.
Miller, G., Suzuki, N., Ciftci-Yilmaz, S., Mittler, R., 2010. Reactive oxygen species homeostasis and signaling during drought and salinity stresses. Plant, Cell and Environment. 33, 453-467.
Moameni, A., 2011. Geographical distribution and salinity levels of soil resources of Iran. Iranian Journal of Soil Research. 24, 203- 215. [In Persian with English summary].
Moieni, A., Mirzaee, M., Ghanati, F., 2013. Effects of drought stress on the lipid peroxidation and antioxidant enzyme activities in two canola (Brassica napus L.) cultivars. Journal of Agricultural Science and Technology. 15, 593-602. [In Persian with English summary].
Ohsugi, R., Hirochika, H., Ichikawa, H., Komatsu, S., AoKi, N., Nakamura, H., Hakata, M., Asano, T., 2011. Functional characterization of OsCPK21 a calcium dependent protein kinase that confers salt tolerance in rice. Plant Molecular Biology. 75, 179-191.
Morales, J.M.L., Rodríguez-Monroy, M., Sepúlveda-Jiménez, G., 2012. Betacyanin accumulation and guaiacol peroxidase activity in Beta vulgaris L. leaves following copper stress. Acta Societatis Botanicorum Poloniae. 81(3).
Noctor, A., Mhamdi, A., Queval, G., Chaouch, S., Vanderauwera, S., Van Breusegem, F., 2010. Catalase function in plants: a focus on Arabidopsis mutants as stress-mimic models. Journal of Experimental Botany. 61(15), 4197–4220.
Omidi, H., 2010. Changes of proline content and activity of antioxidative enzymes in two canola genotype under drought stress. American Journal of Plant Physiology. 5(6), 338-349.
Pfaffl, M.W., 2001. A new mathematical model for relative quantification in real-time RT–PCR. Nucleic acids research. 29(9), 45.
Polle, A., Otter, T., Seifert, F., 1994. Apoplastic Peroxidases and Lignification in Needles of Norway Spruce (Picea abies L.). Plant Physiology. 106(1), 53-60.
Rezaei, H., Khosh, S.K., Malakouti, M.J., Pessarakli, M., 2006. Salt tolerance of canola in relation to accumulation and xylem transportation of cations. Journal of Plant Nutrition. 29, 1903-1917.
Sairam, R.K., Rao, K.V., Srivastava, G.C., 2002. Differential response of wheat genotypes to long-term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Science. 163, 1037–1046.
Sanders, D., Pelloux, J., Brownlee, C., Harper, J.F., 2002. Calcium at the crossroads of signaling. Plant Cell. 14, 401–417.
Shrivastava, P., Kumar, R., 2015. Soil salinity: A serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. Saudi Journal of Biological Sciences. 22, 123–131.
Tai, S.h., Liu, G.S., Sun, Y.h., Chen, J., 2009. Cloning and Expression of Calcium-Dependent Protein Kinase (CDPK) Gene Family in Common Tobacco (Nicotiana tabacum). Agricultural Sciences in China. 12, 1448-1457.
Wang, H.M., Xiao, X.R., Yang, M.Y., Gao, Z.L., Zang, J., Fu, X.M., Chen, Y.H., 2014. Effects of salt stress on Antioxidant Defense System in the Root of Kandelia candel. Botanical Studies. 55- 57.
Weatherley, P.E., 1950. Studies in the water relations of the cotton plant: I. The field measurement of water deficits in leaves. New Phytologist. 49, 81-97.
Yang, F., Xiao, X., Zhang, S., Korpelainen, H., Li, C., 2009. Salt stress responses in Populus cathayana Rehder. Plant Science. 176(5), 669–677.
You, J., Chan, Z., 2015. ROS Regulation during Abiotic Stress Responses in Crop Plants. Frontiers in Plant Science. 6(1092). doi: 10.3389/fpls.2015.01092
Zahedi, H., Tohidi Moghadam, H.R., 2011. Effect of drought stress on antioxidant enzymes activities with zeolite and selenium application in canola cultivars. Research on Crops. 12(2), 388-392.
Zare, S., Abili, J., 2014. Evaluation of antioxidant enzymes activity in canola under salt stress. International Journal of Farming and Allied Sciences. 3(7), 767-771.
Zhang, D.P., Zhang, X.Z., Xu, Y.H., Wu, F.Q., Wang, X.F., Du, S.Y., Shang, Y., Fan, R.C., Li, Y., Zho, R., Wang, X.J., Yu, X.C., Zhu, S.Y., 2007. Two calcium dependent protein kinase CPK4 and CPK11 regulate abscisic acid signal transduction in Arabidopsis. Plant Cell. 19, 3019-3036.
Zhang, H., Liu, W. Z., Zhang, Y., Deng, M., Niu, F., Yang, B., Wang, X., Wang, B., Liang, W., Deyholos, M.K., Jiang, Y.Q., 2014. Identification, expression and interaction analyses of calcium-dependent protein kinase (CPK) genes in canola (Brassica napus L.). BMC Genomics. 15, 211.
Zhou, Y., Xu, D., Jia, L., Huang, X., Ma, G., Wang, S., Zhu, M., Zhang, A., Guan, M., Lu, K. and Xu, X., 2017. Genome-wide identification and structural analysis of bZIP transcription factor genes in Brassica napus. Genes. 8(10), 288.
Zhu, J.K., 2001. Plant salt tolerance. Trends in Plant Science. 6(2), 66–72.
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