ارزیابی اثر قارچ میکوریزا Glomus fascollaria بر برخی خصوصیات مورفولوژیک، رنگیزه‌های نورساختی و فعالیت آنزیم های آنتی‌اکسیدانی کاسنی (.Cichorium intybus L) تحت تنش خشکی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل

2 استاد، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل

3 استادیار، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل

چکیده

به‌منظور بررسی اثر قارچ میکوریزا بر برخی خصوصیات مورفولوژیک، رنگیزه­ های نورساختی و ویژگی‌های آنتی‌اکسیدانی گیاه کاسنی تحت تنش خشکی آزمایشی به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار در گلخانه پژوهشکده کشاورزی دانشگاه زابل انجام شد. عوامل آزمایشی شامل تنش خشکی در چهار سطح 90، 70، 50 و 30 درصد ظرفیت زراعی به­ ترتیب به‌عنوان شاهد، تنش ملایم، تنش متوسط و تنش شدید و تیمار با قارچ میکوریزا در دو سطح تلقیح با قارچ میکوریزا و عدم تلقیح با قارچ میکوریزا بود. در این بررسی وزن تر و خشک اندام هوایی و ریشه، محتوای رنگیزه­ های فتوسنتزی و آنزیم­ کاتالاز، پلی فنل اکسیداز و گایاکول پراکسیداز مورد ارزیابی قرار گرفتند. نتایج نشان داد که بیشترین میزان وزن تر وخشک اندام هوایی متعلق به تنش 90 درصد و تلقیح با قارچ میکوریزا بود و بیشترین میزان کلروفیل a، b، کلروفیل کل و کارتنوئید متعلق به تنش 90 درصد و تلقیح با قارچ میکوریزا بود. حداکثر فعالیت آنزیم کاتالاز، پلی فنل اکسیداز و گایاکول پراکسیداز متعلق به تیمار تنش خشکی 30 درصد ظرفیت زراعی و تلقیح با قارچ میکوریزا و کمترین میزان آن در گیاه شاهد بود. از این آزمایش می‌توان نتیجه گرفت قارچ میکوریزا آربوسکولار به دلیل افزایش فعالیت آنزیم‌های آنتی­ اکسیدانی و افزایش رنگیزه‌های فتوسنتزی خطرات ناشی از تنش خشکی بر رشد و عملکرد گیاه کاسنی را کاهش می‌دهد.

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