اثر محلول پاشی اسید هیومیک بر برخی ویژگی های مورفوفیزیولوژیکی و بیوشیمیایی نعناع سبز (.Mentha spicata L) تحت تنش خشکی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

2 دانشیار گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

3 دانشجوی کارشناسی ارشد گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

چکیده

به منظور بررسی اثرات تعدیل‌کنندگی اسید هیومیک بر تنش خشکی در گیاه نعناع سبز، آزمایشی گلدانی به صورت فاکتوریل در قالب طرح کاملا تصادفی با سه تکرار اجرا شد. تیمارهای آزمایش شامل تنش خشکی در سه سطح (30، 70 و 100 درصد ظرفیت زراعی) به عنوان فاکتور اول و محلول‌پاشی اسید هیومیک در چهار سطح (صفر، 100، 500 و 1000 میلی‌گرم در لیتر) به عنوان فاکتور دوم بودند. نعناع سبز قادر نبود بیش از 2 هفته تنش 30 درصد ظرفیت زراعی را تحمل کند و به طور کامل گیاهان این سطح نابود شدند. صفات رویشی گیاه از قبیل ارتفاع بوته، تعداد شاخه فرعی، طول برگ، عرض برگ و وزن تر و خشک برگ و ساقه در تنش ملایم خشکی (70 درصد ظرفیت زراعی) کاهش معنی‌داری نسبت به تیمار شاهد نشان دادند. محلول‌پاشی اسید هیومیک به ویژه غلظت 1000 میلی‌گرم در لیتر تحت تنش خشکی سبب بهبود این صفات نسبت به گیاهان شاهد گردید. بیشترین مقادیر رنگیزه‌های فتوسنتزی در تنش خشکی 70 درصد ظرفیت زراعی و محلول‌پاشی 1000 میلی‌گرم در لیتر اسید هیومیک و کمترین آنها در تیمار عدم تنش خشکی و کاربرد 500 میلی‌گرم در لیتر اسید هیومیک مشاهده شد. بالاترین میزان فنل کل (0.62 میلی‌گرم/گرم وزن تر) و فعالیت آنتی‌اکسیدانی عصاره برگ (60.16 درصد) در گیاهان حاصل از محلول‌پاشی 1000 میلی‌گرم در لیتر‌ اسید هیومیک تحت تنش ملایم خشکی مشاهده شد. بیشترین محتوی رطوبت نسبی در شرایط عدم تنش و محلول‌پاشی 1000 میلی-گرم در لیتر اسید هیومیک حاصل شد. بنابراین می‌توان'گفت که نعناع سبز گیاهی بسیار حساس به تنش خشکی است و با توجه به کاهش ‌شاخص‌های رشد و فرایندهای فیزیولوژیکی ناشی از تنش خشکی کاربرد اسید هیومیک به‌ویژه در غلظت بالا بصورت محلول‌پاشی می‌تواند به عنوان کود آلی همراستا با کشاورزی پایدار در تنظیم پتانسیل اسمزی نعناع سبز تحت شرایط تنش خشکی ملایم تا حدودی موثر باشد.

کلیدواژه‌ها


Abbaszadeh, B., Sharifi Ashourabadi, E., Lebaschi, M.H., Naderi Hajibagher Kandy, M., Moghadami, F., 2007. The effect of drought stress on proline contents, soluble sugars, chlorophyll and relative water contents of balm (Melissa officinalis L.). Iranian Journal of Medicinal and Aromatic Plants. 23(4), 504-513. [In Persian with English summary].

Abedini, T., Moradi, P., Hani, A., 2015. Effect of organic fertilizer and foliar application of humic acid on some quantitative and qualitative yield of pot marigold. Journal of Novel Applied Sciences. 4(10), 1100-1103.

Ahmad, Y.M., Shahlaby, E.A., Shnan, N.T., 2011. The use of organic and inorganic cultures in improving vegetative growth, yield characters and antioxidant activity of roselle plants (Hibiscus sabdariffa L.). African Journal of Biotechnology 10(11), 1988-1996.

Andre, C.M., Schafleitner, R., Legay, S., Lefevre, I., Aliaga, C.A.A., Nomberto, G., Hoffmann, L., Hausman, J.F., Larondelle, Y., Evers. D., 2009. Gene expression changes related to the production of phenolic compounds in potato tubers grown under drought stress. Phytochemistry. 70(90), 1107-1116.

Ayas, H., Gulser, F., 2005. The effect of sulfur and humic acid on yield components and macronutrient contents of spinach. Journal of Biological Sciences. 5(6), 801-804.

Babaian, M., Heidari, M., Ghanbari, A., 2008. Effects of foliar micronutrient application on osmotic regulation, yield and yield components of sunflower seeds var. Alster during of three stages of drought. Science and Technology of Agriculture and Natural Resources. 12, 119-128. [In Persian with English summary].

Barrs, H.D., Weaterley, P.E., 1962. A re-examination of the relative turgidity techniques for the estimating water deficit in leaves. Australian Journal of Biological Sciences. 15, 413-428.

Bates, I.S., Waldern, R.P., Terare, I.D., 1973. Rapid determination of free proline for water stress studies. Plant and Soil. 39, 205-207.

Bhatt, R.M., Srinvasa Roa, N.K., 2005. Influence of pod load response of okra to water stress. Indian Journal of Plant Physiology. 10, 54-59.

Chimenti. C., 2000. Activity and mechanism of action of natural antioxidants in lipids. Recent Research, Development Oil Chemistry. 2, 1-14.

Chamani, A., Bonyadi, M., Ghanbari, A., 2014. Effect of salicylic acid and humic acid on growth indices of ornamental plant (Catharanthus roseus L.). Journal of Horticultural Science. 4, 631-641.

Delfine, S., Togenetti, R., Desiderio, E., Alivino, A., 2005. Effect of foliar application of N and humic acids on growth and yield of durum wheat. Agronomy for Sustainable Development. 25, 183-191.

Ders, S., Gunes, T., Sivaci, R., 1998. Spectrophotometric determination of chlorophyll a, b and total carotenoids contents of some algae species using different solvents. Journal of Botany. 22, 13-17.

Du, Y.C., Nose, A., Wasano, K., Uchida, Y., 1998. Response to water stress of enzyme activities and metabolite levels in relation to sucrose and starch synthesis, the Calvin cycle and the C4 pathway in sugarcane (Saccharum sp.) leaves. Functional Plant Biology. 25, 253-260.

De Feo, V., Ricciardi, A.I., Biscardi, D., Senatore, F., 1998. Chemical composition and antimicrobial screening of the essential oil of Minthostahys verticillate (Griseb) Ep1 (Lamiceae). Journal of Essential Oil Research. 10(1), 61-65.

Fu, J., Haung, B., 2001. Involvement of antioxidants and lipid peroxidation in the adaptation two cool seasons to localized drought stress. Environmental and Experimental Botany. 45, 105-114.

Guvence, I., Dursun, A., Turan, M., Tuzel, Y., Burrage, S.W., Bailey, B.J., Gul, A., Smith, A.R., Tuncay, O., 1999. Effect of different foliar fertilizers on growth, yield and nutrient content of lettuce and crisp lettuce. Acta Horticulturae. 491, 247-252.

Ghorbanli, M., Bakhshi Khaniki, Gh., Zakeri, A., 2011. Investigation on the effects of water stress on antioxidant compounds of Linum usitatissimum L. Iranian Journal of Medicinal and Aromatic Plants. 27(4), 647-658. [In Persian with English summary].

Heidari, M., Reza Miri, H., Minaie, A., 2015. Activities of antioxidant enzymes and biochemical compounds of borage plant (Borago officinalis) in response to drought stress treatments and humic acid. Journal of Environmental Stresses in Crop Sciences. 2(6), 159-170. [In Persian with English summary].

Izadi, Z., Asnaashari, M., Ahmadvand, G.H., 2009. The effect of drought stress on yield, proline, soluble sugars, relative water content and the essential oil of peppermint (Mentha piperita). Iranian Horticultural Science and Technology. 10(3), 223-234. [In Persian with English summary].

Kumar, P., Mishra, S., Malik. A., Satya, S., 2011. Insecticidal properties of Mentha species: A review. Industrial Crops and Products. 34(1), 802-817.

Kim, B.J., Kim, J.H., Kim, H.P., Heo, M.Y., 1997. Biological screening of 100 plant extracts for cosmetic use (II): Antioxidative activity and free radical scavenging activity. International Journal of Cosmetic Science. 19(9), 299-307.

Lotfi, M., Abbaszadeh, B., Mirza. M., 2014. The effect of drought stress on morphology, proline content and soluble carbohydrates of tarragon (Artemisia dracunculus L.). Iranian Journal of Medicinal and Aromatic Plants. 1, 19-29 [In Persian with English summary].

Malcolm, R.E., Vaghuan, D.V., 1979. Humic substance and phosphates actives in plant tissues. Soil Biology and Biochemistry. 11, 253-259.

Monakhova, O.F., Chernyad’ev, I.I., 2002. Protective role of kartolin-4 in wheat plants exposed to soil drought. Prikladnaia Biochemiia Microbiologiia. 38, 373-380.

Marcum, K.B., 1998. Cell membrane the removability and whole-plant heat tolerance of Kentucky bluegrass. Crop Science. 38, 1214-1218.

Mirhajian, A., 2012. What is humic acids? News, Analysis, Training and Agricultural Engineering of Monthly. 33, 7-16.

Mokhtari, A., Baradaran, R., 2011. Effect of drought stress on some growth indices of Satureja hortensis. The First Regional Conference on Ecophysiology of Crop Plants. Islamic Azad University of Shushtar. Shushtar, Iran. 317-319. [In Persian].

Moon, J.H., Terao, J., 1998. Antioxidant activity of caffeic acid and dihydrocaffeic acid in lard and human low-density lipoprotein. Journal of Agriculture and Food Chemistry. 46, 5062-5065.

Munns, R., 2002. Comparative physiology of salt and water stress. Plant, Cell and Environment. 25, 239-250.

Mozafari, S., Khorasani Nejad., Ghorgin Shabankareh, H., 2017. The effect of irrigation regimes and the application of humic acid on some of the physiological and biochemical characteristics of purpura herb in greenhouse conditions. Journal of Crop Improvement. 19(2); 401-416. [In Persian with English summary].

Nassotti Miandab, R., Samawat, S., Tehrani, M.M., 2000. Properties of humic acid on plant and soil. Agriculture and Food. 101, 53-55.

Nardi, S., Pizzeghello, D., Muscolo, A., Vianello, A., 2002. Physiological effects of humic substances on higher plants. Soil Biology and Biochemistry. 34, 1527– 36.

Omidbigi, R., 2007. Production and Processing of Medicinal Plants. Second volume. Astan Quds Razavi Publishing. 438 pp. [In Persian].

Perez-Murcia, M.D., Moral, R., Moreno-Caselles, J., Perez-Espinosa, A., Paredes, C., 2006. Use of composted untreated waste municipal in growth media for broccoli. Journal of Bioresource and Technology. 97, 123-130.

Parvazi Shandi, S., Puzkhi, A., Asgharzadeh, A., Azadi, A., Pazhnezhad, F., 2013. The Effect of Humic Acid Irrigation and Growth Stimulating Bacteria on Physiological Characteristics of Wheat Cultivar in the City of Ray. Crops Physiology Journal. 17, 19-33. [In Persian with English summary].

Rajinder, S.D., 1987. Glutahione status and protein synthesis during drought and subsequent dehydration in Torula rulis. American Society of Plant Physiologists. 83, 816-819.

Rassam, G., Dadkhah, A., Khoshnod Yazdi, A., 2014. Estimation of the impact of water shortages on morphological and physiological traits of herb hyssop. Journal of Agriculture. 10, 1-12. [In Persian with English summary].

Salehi, M., Nassiri Mahallati, A., Koocheki, M., 2003. Leaf nitrogen and chlorophyll as indicators for salt stress. Iranian Journal of Field Crops Research. 1(2), 199-205. [In Persian with English summary].

Sanjari Mianjye, M., Cirrus Mehr, A., Fakhery, B., 2015. Effect of drought stress on some physiological characteristics of Hibiscus sabdarifa. Journal of Crop Improvement. 17(2), 403-414. [In Persian with English summary].

Sardashti, A., Alidoust, A., 2007. Identify the compounds of humic acid in forest soils in northern Iran. Fifteenth Congress of Crystallography and Mineralogy of Iran. Ferdowsi University of Mashhad, Mashhad. 361 pp.

Schutz, M., Fangmeier, A., 2001. Growth and yield responses of spring what (Triticum aestivum L. cv. Minaret) to elevated CO2 and water limitation. Environmental Pollution. 114, 187-194.

Salahvarzi, Y., Tehranifar, A., Ghazanchian, A., 2008. Investigation of physiomorphologic changes of native and foreign green foliage, in drought stress and irrigation again. Iranian Journal of Horticultural Engineering. 9, 193-204. [In Persian with English summary].

Sharif, M., Riaz, A., Khattak, M., Sarir, M., 2002. Effect of lignitic coal derived humic acid on growth of maize Plants. Communications in Soil Science and Plant Analysis. 33, 3567-3580.

Shrififar, F., Moshafi, M.H., Mansouri, S.H., 2007. In vitro evaluation of antibacterial and antioxidant of the essential oil and methanol extract of endemic Zataria multiflora Boiss. Food Control. 18, 800-805.

Singleton, V.L., Rossi, J.A., 1965. Colorimetry of total phenolics with phoshomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture. 16, 144-158.

Smirnoff. N., 1993. The role active oxygen in the response of plants to water deficit and desiccation. New Phytologist. 125, 27-28.

Sultana, N., Ikeda, T., Itoh, R., 1999. Effect of NaCl salinity on photosynthesis and dry matter accumulation in developing rice grains. Environmental and Experimental Botany. 42(3), 211-220.

Tian, X., Lei, Y., 2006. Nitric oxide treatment alleviates drought stress in wheat seedlings. Biologia Plantarum. 50(4), 775-778.

Tarahomy, GH., Lahooti, M., Abbasi, F., 2010. The effect of drought stress on soluble sugars, chlorophyll and potassium S. leriifolia Journal of Biological Sciences, Islamic Azad University of Zanjan. 3(2), 1-7. [In Persian with English summary].