اثر پیش تیمار بذر لوبیا (.Phaseolus vulgaris L) با پرولین و گلایسین بتائین در تحمل به تنش سرب

نوع مقاله: مقاله پژوهشی

نویسنده

دانشیار گروه زراعت، واحد یادگار امام خمینی (ره) شهر ری، دانشگاه آزاد اسلامی، تهران ، ایران

چکیده

به‌منظور بررسی مقایسه‌ای اثر پیش‌تیمار بذر لوبیا چیتی با پرولین و گلایسین بتائین در تحمل به تنش سرب آزمایشی گلدانی در بهار و تابستان سال 1394 انجام شد. این تحقیق به صورت طرح کاملاً تصادفی با شش تیمار و چهار تکرار اجرا گردید. تیمارها عبارت بودند از: شاهد، تنش سرب (500 میلی‌گرم بر کیلوگرم خاک نیترات سرب)، تنش سرب + پیش‌تیمار بذر با محلول 25 میلی-مولار پرولین، تنش سرب + پیش‌تیمار بذر با محلول 50 میلی‌مولار پرولین، تنش سرب + پیش‌تیمار بذر با محلول 25 میلی‌مولار گلایسین بتائین و تنش سرب + پیش‌تیمار بذر با محلول 50 میلی‌مولار گلایسین بتائین. نتایج نشان داد که تنش سرب موجب افزایش غلظت سرب ریشه و خسارت اکسیداتیو به بوته‌های لوبیا گردید. سمیت سرب همچنین موجب کاهش شاخص سبزینگی، محتوی نسبی آب، ارتفاع بوته، سطح برگ و زیست توده ریشه و اندام هوایی شد. با این وجود، در شرایط تنش سرب، پیش‌تیمار بذر با پرولین یا گلایسین بتائین موجب کاهش جذب سرب، افزایش فعالیت آنزیم‌های آنتی اکسیدانت و کاهش خسارت اکسیداتیو گردید که باعث بهبود شاخص سبزینگی، محتوی نسبی آب، ارتفاع بوته، سطح برگ و زیست توده ریشه و اندام هوایی شد. بین تیمارهای مختلف کاربرد پرولین و گلایسین بتائین اختلاف معنی‌داری مشاهده نشد. براساس یافته‌های این پژوهش، پیش‌تیمار بذر با پرولین یا گلایسین بتائین می‌تواند از طریق کاهش جذب سرب، افزایش فعالیت آنزیم‌های آنتی اکسیدانت و بهبود شاخص سبزینگی و وضعیت آبی گیاه، به عنوان روشی مفید در بهبود تحمل به تنش سرب در لوبیا مورد استفاده قرار گیرد.

کلیدواژه‌ها


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