اثر محلول پاشی اسید سالیسیلیک و اسید جاسمونیک بر قندهای محلول، پرولین و آنزیم های اسطوخودوس (Lavendula officinalis L.) تحت تنش شوری

نوع مقاله: مقاله پژوهشی

نویسندگان

گروه زراعت، واحد یادگار امام خمینی (ره) شهرری، دانشگاه آزاد اسلامی، تهران، ایران

چکیده

شوری خاک از عوامل محدود­کننده عملکرد محصولات کشاورزی ازجمله گیاهان دارویی است و به­ عنوان یکی از اساسی­ترین مشکلات کشاورزی محسوب می­شود. ازاین‌روی، به ­منظور بررسی اثر اسید سالیسیلیک و اسید جاسمونیک بر قند­های­ محلول، پرولین و فعالیت آنزیم­های آنتی ­­اکسیدان گیاه دارویی اسطوخودوس (Lavendula officinalis L.) در شرایط تنش شوری، آزمایشی گلدانی، در سال 1393 در دانشگاه آزاد اسلامی واحد یادگار امام خمینی (ره) شهرری و گلخانه واقع در منطقه 4 به مرحله اجرا در­آمد. آزمایش به ­صورت فاکتوریل در قالب طرح کاملاً تصادفی با چهار تکرار اجرا گردید که در آن تنش شوری از منبع نمک طعام (NaCl)، در چهار سطح (0، 25، 50 و 75 میلی­ مولار)، اسید سالیسیلیک در دو سطح (0 و 0.7 میلی­ مولار) و اسید جاسمونیک در دو سطح (0 و 100 میکرو­مولار) در نظر گرفته شد. نتایج حاصل از مقایسه میانگین اثرات ساده شوری نشان داد که این عامل سبب افزایش قندهای محلول(%59) و پرولین (%76)، فعالیت آنزیم­های آنتی ­­اکسیدان ازجمله؛ کاتالاز (%72)، سوپراکسید ­­دیسموتاز (%67)، پراکسیداز (%63)، آسکوربات ­پراکسیداز (%60) و گلوتاتیون ­پراکسیداز (%72) شد. همچنین نتایج حاصل از پژوهش نشان داد، تنها اثر متقابل سه­ گانه عوامل آزمایشی بر صفات پراکسیداز، آسکوربات ­­پراکسیداز معنی­ دار گردید. در این شرایط بیشترین میزان فعالیت پراکسیداز (50.27) میلی­گرم پروتئین بر دقیقه و آسکوربات­ پراکسیداز (24.12) میلی­ گرم پروتئین بر دقیقه، در عامل شوری 75 میلی­ مولار و عدم حضور دو ترکیب مذکور حاصل گردید. به‌طورکلی، به نظر می­رسد که استفاده از اسید سالیسیلیک و اسید جاسمونیک از طریق افزایش فعالیت قندهای محلول و پرولین باعث افزایش مقاومت گیاه در شرایط تنش شوری گردید.

کلیدواژه‌ها


Abdala, G., Miersch, O., Kramell, R., Vigliocco, A., Agostini, E., Forchetti, G., Alemano, S., 2003. Jasmonate and octadecanoid occurrence in tomato hairy roots Endogenous level changes in response to NaCl. Plant Growth Regulation. 40, 21-27.

Akhondi, M., Safarnejad, E., Lahooti, M., 2006. Effect of drought stress on proline and accumulation of ions. Journal of Agricultural Science. 10, 165-173. [In Persian with English Summary].

Ashraf, M., Ali, Q., 2008. Relative membrane permeability and activities of some antioxidant enzymes as the key determinants of salt tolerance in canola (Brassica napus L.). Environmental and Experimental Botany. 63, 266-273.

Berrington D., Lall N., 2012. Anticancer activity of certain herbs and spices on the cervical epithelial carcinoma (HeLa) cell line. Journal of Evidence-Based Complementary and Alternative Medicine. 564-927.

Cakmak, I., Horst W., 1991. Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase and peroxidase activities in root tip of soybean (Glysin max). Journal of Plant Phisiology. 83, 463-468.

Chen, Z., Ricigliano JR., Klessig D.F., 1993. Purification and characterization of a soluble salicylic acid binding protein from tobacco. Journal of Proceedings of the National Academy of Sciences of United State of America. 90, 9533-9537.

Comparot, S.M., Graham, C.M., Reid, D.M., 2002. Methyl jasmonate elicits a differential antioxidant response in light and dark grown canola (Brassica napus) roots and shoots. Journal Plant Growth Regulation. 38, 21-30.

Conrath, U., Pieterse, C.M.J., Mauch-Mani, B., 2002. Priming in plant–pathogen interactions. Trends in Plant Science. 7, 210-216.

Dadman, B., Omidbeygi, R., Sefidkan, F., 2007. Effect of nitrogen on essential oil of Mexican parsley. Journal of Iranian Aromatic and Medicinal Plants Research. 23(4).38, 484 - 91. . [In Persian with English Summary].

Dixit, V., Pandey, V., Shyam, R., 2001. Differential antioxidative responses to cadmium in roots and leaves of pea (Pisum sativum). Experimental Botany. 52, 1101-1109.

Doulatabadian, A., Modarres Sanavi, S.A.M., Etemadi, F., 2008. Effec of pretreatment of salicylic acid on wheat (Trittcum aestivum L.) seed germination under salt stress.Iranian Journal of Plant Biology. 692-702. [In Persian with English Summary].

Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F., 1956. Colorimetric method for determination of sugars and related substances. Annual Chemistry. 28, 350-356

Fakhari, A., Salehi, P., Heydari, R., Nejad Ebrahimi, S., Haddad, P., 2005. Hydrodistillation headspace solvent microextraction; a new method for analysis of the essential oil components of Lavandula angustifolia Mill. Journal of Chromatography A. 1098(1-2), 14-78.

Fedina, I.S., Benderliev, K.M., 2000. Response of secendesmus incrassatulus to salt stress as affected by methyl jasmonate. Biologica Plantarum. 43(4), 625-627.

Ferrarese, NG,P.L.L., M.L.L., Huber, D.A., Ravagnani, A.L.S., Ferraree –Filho, O., 2003. Conola (Brassica napus L.) seed effects on germination of Glycine max effects of cinnamic acid and benzoic acids and derivatives. Seed Science and technology. 31, 39-48.

Ghanati, F., Morita A., Yokota H., 2002. Induction of suberin and increase of liginin content by exess Boron in Tabacco cell. Soil Science and Plant Nutrition. 48(3), 357-364.

Giannopolitis, C., Ries, S., 1997. Superoxid desmutase. I. Occurence in higher plant. Plant Physiology. 59, 309-314.

Hernandez, J.A., Ferrer, M.A., Jimenez, A., Barcelo, A.R., Sevilla, F., 2001.
Antioxidant systems and O2-/ H2O2 production the apoplast of pea leaves. Its
relation with salt- induced necrotic lesions in minor veins. Plant Physiology.
127, 827-831.

Horváth, E., Janda, T., Szalai, G., Páldi, E., 2002. In vitrosalicylic acid inhibition of catalase activity in maize: differences between the isoenzymes and a possible role in the induction of chilling tolerance. Plant Science.163, 1129-1135.

Horvath, E., Szalai, G., Janda, T., 2007. Induction of abiotic stress tolerance by salicylic acid signaling. Plant Growth and Regulation. 26, 290-300.

Jung, S., 2004. Effect of chlorophyll reduction in Arabidopsis thaliana by methyl jasmonate or norflurazon on antioxidant systems. Plant Physiology and Biochemistry. 42, 231-255.

Katerji, N., Mastrorilli, M., van Horn, J.W., Lahmer, F.Z., Hamdy, A., Oweis, T., 2009. Durum wheat and barley productivity in saline -drought environments. European Journal of Agronomy. 31, 1-9.

Keles, Y., Oncel, I., 2004. Growth and solute composition in two wheat species experiencing combined influence of stress conditions. Russian Journal Plant Physiology. 51, 203-208.

Kerepesi, I., Galiba, G., 2000. Osmotic and salt stress Induced alteration in soluble carbohydrate content in wheat seedling. Crop Science. 40(2), 482-487.

Kshavrz, H., Modares Sanavi, S.A.M., Zarin Kamr, F., Dolatabadian, A., Panahi, M., Sadaj Asilan, K., 2012. Evolution effect salicylic acid foliar on same traits biochemical two (Brasica napus L.) under cool stress. Iranian Agricultural Science. 42, 723-734.

Matysik, J., Alia Balu, B., Mohanty, P., 2002. Molecular mechanisms of quenching of reactive oxygen species by proline under stress in plants. Current Science. 82, 525-531.

Mittler, R., 2002. Oxidative stress, antioxidant and stress tolerance. Annual Reviews on Plant Science. 7, 405-415.

Mohammdkhani, N., Heidari, R., 2007. Effects of drought stress on protective enzyme activities and lipid peroxidation in two maize cultivars. Pakistan Journal of Biological Sciences. 10 (21), 3835-3840.

Murgan, K., Harish, S.R., 2007. Antioxidant modulation in response to heavy metal induced Oxidative stress in Chodophora glomerata, Indian Journal of Experimental Biological. 45, 980-983.

Neelam, M., Saxena, P., 2009. Effect of salicylic acid on proline metabolism in lentil grown under salinity stress. Plant Science. 177, 181-189.

Omidbeigi, R., 2006. Production and Processing of Medicinal Plants. Beh Nashr Publication. [In Persian].

Paglia, D.E., Valentine, W.N., 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. The Journal ofLaboratory and Clinical Medicine. 70(1), 158-169.

Pan, Y., Wu, L.J., Yu, Z.L., 2006. Effect of salt and drought stress on antioxidant enzymes activities and SOD isoenzymes of liquorice (Glycyrrhiza uralensis Fisch). Plant Growth Regulation. 49, 157-165.

Parvaiz, A., Satyawati, S., 2008. Salt stress and phyto-blochemical responses of plants. Journal of Plant, Soil and Environmental. 54, 89-99.

Patakas, A., Nikolaou, N., Zioziou, E., Radoglou, K., Noitsakis, B., 2002. The role of organic solute and ion accumulation in osmotic adjustment in drought-stressed grapevines. Plant Science. 163, 361- 374.

Ranieri, A., Castagna, A., Baldan, B., Soldatini, G.F., 2001. Iron deficiency differently affects peroxidase isoforms in sunflower. Experimental Botany. 52(354), 25-35.

Sairam, R.K., Chandrasekhar, V., Srivastava, G.C., 2001. Comparison of hexaploid and tetraploid wheat cultivars in their response to water stress. Biologica Plantarum. 44, 89-94.

Sairam, R.K., Veerabhadra Rao, K., Srivastava, G.C., 2002. Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Science. 163, 1037-1046.

Senaranta T., Ouchell D., Bunn E., Dixon, K., 2000. Acetyl salicylic acid (aspirin) and salicylic acid induce multiple stress tolerance in bean and tomato plants. Plant Growth Regulator. 30, 157-161.

Shakirova, F.M., Sakhabutdinova, A.R., Bezrukova, M.V., Fatkhutdinova, R.A., Fatkhutdinova, D.R., 2003. Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity. Plant Science. 164, 317-322.

Tan, Y., Liang, Z.S., Hongbo, H.B., Du, F., 2006. Effect of water deficits on the activity of anti-oxidative enzymes and osmoregulation among three different genotypes of Radix Astragali at graining stage. Colloids and Surfaces Botany Biointerfaces. 49, 60-65.

Wang, X.S., Han, J.G., 2009. Changes of Proline Content, Activity, and Active Isoforms on Antioxidative Enzymes in Two Alfalfa Cultivars under Salt Stress. Journal of Agricultural Sciences in China. 8(4), 431-440.

Wanichan, P., Kirdmanee, C., Vutyano, C., 2003. Effect of salinity on biochemical and physiological characteristics in correlation to selection of salt tolerance in Aromatic rice (Oryza sativa L.). Sciences Asia. 29, 333-330.

Yazdanpanah, S., Abasi, F., Baghzadeh, A., 2010. Effect of salicylic acid and ascorbic acid on proline, sugar and protein content in Satureja hortensis L. under aridity stress. Proceeding of the First National Conference of Environmental Stress in Agricultural Science 28-29 The University of Birjand. [In Persian with English Summary].