مطالعه صفات فیزیولوژیک گیاه دارویی مرزنجوش یک ساله (Origanum majorana) در پاسخ به عنصر روی در شرایط تنش خشکی

نوع مقاله: مقاله پژوهشی


1 دانشجوی کارشناسی ارشد، گروه باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه هرمزگان، بندرعباس، ایران.

2 استادیار، گروه باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه هرمزگان.

3 استادیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه یاسوج.

4 مربی، گروه باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه هرمزگان.


به‌منظور بررسی واکنش فیزیولوژیکی گیاه دارویی مرزنجوش (Origanum majorana) به عنصر روی در شرایط تنش خشکی، آزمایشی به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با 3 تکرار در سال 1393 در گلخانه تحقیقاتی دانشگاه هرمزگان اجرا گردید. فاکتورهای آزمایش شامل سطوح مختلف تنش خشکی (رطوبت خاک معادل 50، 75 و 100% ظرفیت زراعی) محلول‌پاشی کود روی (غلظت­های 0، 1 و 3 در هزار) بودند.  نتایج تجزیه واریانس نشان داد که تنش خشکی اثر معنی­داری بر تمام صفات به‌غیراز درصد اسانس داشته و تیمار کود روی در تمام صفات اندازه­گیری شده به‌غیراز کلروفیل a و آنزیم کاتالاز اثر معنی­داری داشته است. اثر متقابل روی و تنش خشکی بر  میزان کلروفیل a، b، کل، میزان آنتوسیانین و درصد اسانس معنی‌دار بود. اعمال تنش معادل 75% ظرفیت زراعی موجب افزایش کلروفیل a، b و کل گردید درحالی‌که تنش 50% ظرفیت زراعی موجب افزایش میزان کاروتنوئید، قند محلول، آنتوسیانین و آنزیم کاتالاز گردید. محلول ­پاشی غلظت سه در هزار روی موجب افزایش میزان کلروفیل b و کل و محلول ­پاشی غلظت یک در هزار روی نیز موجب افزایش میزان قند محلول و درصد اسانس گردید. در شرایط تنش 75 و 100% ظرفیت زراعی، محلول­پاشی یک در هزار روی موجب افزایش میزان کلروفیل  a، b، میزان آنتوسیانین و درصد اسانس شد. هرچند در این آزمایش آنزیم کاتالاز تحت تأثیر تیمارهای روی قرار نگرفت اما محلول‌ پاشی با غلظت‌ پایین روی از طریق افزایش بیوسنتز کلروفیل، کارتنوئید، آنتوسیانین و قند محلول باعث افزایش تحمل گیاه مرزنجوش و درنتیجه بهبود شرایط رشدی این گیاه در شرایط تنش خشکی شد.


Baatour, O., Kaddour, R., Mahmoudi, H., Tarchoun, I, Bettaieb I., Nasri, N., Mrah, S., Hamdaoui, G., Lachaâl, M., Marzouk, B., 2011. Salt effects on Origanum majorana fatty acids and essential oils composition. Journal of the Science of Food and Agriculture. 10, 1002-4495.

Bahreininejad, B., Razmjoo, J., Mirza, M., 2013. Influence of drought stress on morpho-physiological traits in Thymus daenensis. International Journal of Plant Production. 7(1), 151-166.

Bohnert, K.H., Nelson, D. E., Jensen,R.G., 1995. Adaptations to environmentstresses. The Plant and Cell. 7, 1099-1111.

Cakmak, I., 2000. Possible roles of zinc in protecting plant cells from damage by reactive oxygen species. New Phytologist 146, 185-205.

Candan, N., Tarhan, L., 2003. Changes in chlorophyll-carotenoid contents, antioxidant enzyme activities and lipid peroxidation levels in Zn-stressed Mentha pulegium. Turkish Journal of Chemistry. 27, 21-30.

Charles, D.J., Simon, J. E., 1990. Comparison of extraction methods for the rapid determination of essential oil content and composition of basil. Journal of the American Society for Horticultural Science. 115(3), 458-462.

Choudhury, R.P., Kumar, A., Gary, A. N., 2006. Analysis of Indian mint (Mentha spicata) for essential, trace and toxic elements and its antioxidant behavior, pharmaceutical and biochemical analysis. Journal of Pharmaceutical and Biomedical Analysis. 41(3), 825-32.

Dat, J., Vandenabeele, S., Vranova, E., Van Montagu, M., Inze, D., Van Breusegem, F., 2000. Dual action of the active oxygen species during plant stress responses. Cellular and Molecular Life Sciences. 57, 779–795.

Dazy, M., Jung, V., Ferard, J., Masfaraud, J., 2008. Ecological recovery of vegetation on a coke-factory soil: Role of plant antioxidant enzymes and possible implication in site restoration. Chemosphere. 74, 57-63.

Delaney, A.J., Hu, C. A.A., Kishor, K.P.B., Verma, D.P.S., 1993. Cloning ornithine-aminotransferase cDNA from Vigna anconitifolia by trans-complementation in Escherichia coil and regulation of proline biosynthesis. Journal of Biological Chemistry. 268, 18673-18678.

El-Tayeb, M.A., Ahmed, N.L., 2010. Response of wheat cultivars to drought and salicylic acid. American-Eurasian Journal of Agronomy. 3(1), 01-07.

Ghanati, F., Bakhtiyarian, S., Abdolmaleki, P., 2010. Effects of methyl jasmonate on the secondary metabolites of Calendula officinalis L. Biotechnology (Tarbiat Modares University). 1(1), 20-30. [In Persian with English Summary].

Ghani dehkordi, F., Ghasemi Pirbaloti, A., Hamidi, B., Malekpoor, F., 2011. Effect of different level water and nitrogen on morphological and physiological traits of Matricaria aurea L. Journal of Herbal Drugs. 2, 101 -111.

Ghorbanli, M. Bakhshi Khaniki, G, Salimi Elizei, S., Hedayati, M., 2010. Effect of water deficit and its interaction with ascorbate on proline, soluble sugars, catalase and glutathione peroxidase amounts in Nigella sativa L. Iranian Journal of Medicinal and Aromatic Plants. 26(4), 466-476. [In Persian with English Summary].

Ghorbanli, M. Bakhshi Khaniki, G.,Zakeri, A., 2012. Investigation on the effects of drought stress on antioxidant compounds of Linum usitatissimum L. Iranian Journal of Medicinal and Aromatic Plants. 27(4), 647-658. [In Persian with English Summary].

Grejtovsky A, Markusova K., Eliasova, A., 2006. The response of chamomile (Matricaria chamomilla L.) plants to soil zinc supply. Plant, Soil and Environment. 52, 1–7.

Hasani, A., 2006. Effects of water dificit stress on growth, yield and essential oil content ofDracocephalum moldavica. Iranian. Journal of Medicinal and Aromatic Plants. 22(3), 256-261. [In Persian with English Summary].

Hasani, A., Omidbaigi, R., 2002. The effect of drought stress on morphological characteristics, physiological metabolites basil. Journal of Knowledge Agricultural. 12(3), 47-59. [In Persian with English Summary].

Inze, D., Montagu, M. V., 2000. Oxidative stress in plants. TJ International Ltd, Padstow, Cornawall. Great Britain. 321 pp.

Jafarzadeh L., Omidi H., Bostani A.A., 2014. The study of drought stress and bio fertilizer of nitrogen on some biochemical traits of Marigold medicinal plant (Calendula officinalis L.). Journal of Plant Research (Iranian Journal of Biology). 27 (2), 180-193. [In Persian with English Summary].

Jiang, Y., Huang, N., 2001. Drought and heatstress injury to two cool-season turfgrasses in relation to antioxidant metabolism and lipid peroxidation. Crop Science. 41, 436-442.

Kafi, M., Mahdavi Damghani, A., 2000. Mechanisms of environmental stress Resistance in Plants. University of Ferdowsi Mashhad Press. 472p. [In Persian]

Khalid, K.A., 2006. Influence of drought stress on growth, essential oil, and chemical compositionof herbs (Ocimum sp.). International Agrophysics. 20, 289-296.

Lotfi, M., Abbaszadeh, B., Mirza, M., 2014. The effect of drought stress on morphology, proline content and soluble carbohydrates of tarragon (Artemisia dracunculus L.). Iranian Journal of Medicinal and Aromatic Plants. 30(1), 19-29. [In Persian with English Summary].

Malakouti, M. J., Moshiri, F., Ghaibi, M. N., Molavi, S., 2005. Optimum Levels of Some Nutrients in Soils and Some Agronomic and Horticultural Crops. Sana Publication. Tehran. 22p. [In Persian].

Mihalovic, N., Lazarevic, M., Dzeletoric, Z., Vuckoric, M., Durde, Vic. M., 1997. Chlorophyllase activity in wheat (Triticum aestivum L.) leaves during drought and its dependence on the nitrogen ion from applied. Plant Science. 129, 141- 146.

Misra, A. 1992. Effect of zinc stress in Japanese mint as related to growth, photosynthesis, chlorophyll content and secondary plant products-the monoterpenes. Photosynthitica. 26, 2225-2234.

Movahhedi Dehnavi, M. 2004. Effect of foliar application of micronutrients (zinc and manganese) on the quantitative and qualitative yield of different autumn safflower cultivars under drought stress in Isfahan. PhD thesis. Faculty of Agriculture, Tarbiat Modarres University, Tehran. [In Persian with English Summary].

Munns, R., 1993. Physiological process limiting plant growth in saline soil: some dogmas andhypotheses. Plant, Cell and Environment. 16, 15-24.

Munns, R. 2002. Comparative Physiology of Salt and Drought stress. Plant Cell and Environment. 25, 239-250.

Omidbaigi, R., 2005. Production and Processing of Medicinal Plants. Astan Quds Publication, Mashhad, Iran. 347p. [In Persian].

Pagter M., Bragato C., Brix, H., 2005. Tolerance and physiological responses of Phragmites australis to waterdeficit. Aquatic Botany. 81, 285-299.

Parida, A.K., Das, A.B., 2005. Salt tolerance and salinity effects on plants: a review. Ecotoxicology and Environmental Safety. 60, 324-349.

Prasad, T.K., 2003. Mechanisms of chilling-induced oxidative stress injury and tolerance in developing maize seedlings: changes in antioxidant system, oxidation of proteins and lipids, and protease activities. The Plant Journal.10, 1017–1026.

Penka, M., 1978. Influence of irrigation on the contents of effective substances in officinal plants. Acta Horticulture. 73,181-198.

Rassam, G. Dadkhah, A., Khoshnood Yazdi, A., 2014. Evaluation of water deficit on morphological and physiological traits of hyssop (Hyssopus officinalis L.). Journal of Agronomy Sciences. 5(10), 1-12.

Ravi, S., Channal, H. T., Hebsur, N. S., Patil, B. N., Dharmatti, P. R., 2008. Effect of sulphur, zinc and iron nutrition on growth, yield, nutrient uptake and quality of safflower (Carthamus tinctorius L). Karnataka Journal of Agricultural Science. 32, 382-385.

Refaat, A. M., Saleh, M. M., 1997. The combined effect of irrigation intervals and foliar nutrition on sweet basil plants. Bulletin of Faculty of Agriculture University of Cairo. 48, 515-527.

Rezai, H., Ghorbanli, M., 2012. Effect of drought stress and its interaction with ascorbic acid on the photosynthetic pigments in plant Moldavian balm (Dracocephalum moldavica L). Abstracts of the NCNPMP. National Conference of Natural Products and Medicinal Plants. Birjand, Iran. 27-28 September. [In Persian].

Rion, B., Alloway, J., 2004. Fundamental aspectsof Zinc in soils and plants. International ZincAssociation Publisher, Paris, France, 128 p.

Safikhani, F., Heydarye Sharifabadi, H., Syadat, A., Sharifi ashorabadi, A., Syednedjad, M., Abbaszadeh, B., 2007. The effect of drought on yield and morphologic characteristics of Deracocephalum moldavica L. Iranian Journal of Medicinal and Aromatic Plants. 23(2), 183-194. [In Persian with English Summary].

Said-Al Ahl H.A.H., Hussein M.S., 2010. Effect of drought stress and potassium humate on the productivity oforegano plant using saline and fresh water irrigation. Ozean Journal of Applied Sciences. 3,125-141.

Schutz, H., Fangmier, E., 2001. Growth and yield responses of spring wheat (Triticum aestivum L. cv. Minaret) to elevated CO2 and water limitation. Environmental Pollution. 114,187-194.

Smirnoff, N., 1998. Ascorbate biosynthesis and function in photoprotection. Philosophical Transactions of the Royal Society of London Series B – Biological Sciences. 355, 1455–1464.

Song, W.Y., Zhang, Z.B., Shao, H.B., Guo, X.L., Cao, H.X., Zhao, H.B., Fu, Z.Y., and Hu, X.J., 2008. Relationship between calcium decoding elements and plant abiotic-stress resistance. International Journal of Biological Sciences. 4(2), 116-125.

Song, C.Z., Liu, M.Y., Meng, J.F., Chi, M., Xi, Z.M., Zhang, Z.W., 2015, Promoting effect of foliage sprayed zinc sulfate on accumulation of sugar and phenolics in berries of Vitis vinifera cv. Merlot growing on zinc deficient soil. Molecules. 20, 2536-2554.

Sperdouli, I., Moustakas, M., 2012. Interaction of proline, sugars, and anthocyanins during photosynthetic acclimation of Arabidopsis thaliana to drought stress. Journal of Plant Physiology. 169, 577-585.

Tahkorpi, M., 2010. Anthocyanins under drought and droughtrelanted stresses in Bilberry (Vaccinium myrtillus L.). Academic dissertation to be presented with the assent of the Faculty of Science of the University of Oulu.

Tewari, R.K., Kumar, P., Sharma, P.N., 2008. Morphology and physiology of zinc-stressed mulberry plants. Journal of Plant Nutrition and Soil Science. 171, 286-294.

Zare Dehabadi, S., Asrar, Z., Mehrabani, M., 2007. Effect of zinc on growth and some physiological and biochemical parameters of spearmint (Mentha spicata L.). Iranian Journal of Biology. 20(3), 230-241. [In Persian with English Summary].

Zare Dehabadi, S., Asrar, Z., 2009. Study on the effects of zinc stress on induction of oxidative stress and concentration of mineral element in spearmint (Mentha spicata L.). Iranian Journal of Biology. 22(2), 218-228. [In Persian with English Summary].

Zehtab- Salmasi, S., Javanshir, A., Omidbaigi, R., Alyari, H., Ghassemi-Golezani, K., 2001. Effects of water supply and sowing date on performance and essential oil production of anise (Pimpinella anisum L). Acta Agronomica Hungarica. 49, 75-81.