تأثیر سالیسیلیک اسید بر برخی خصوصیات مورفولوژیکی، رنگیزه‌های فتوسنتزی و فعالیت آنتی‌اکسیدانی گیاه ریحان (.Ocimum basilicum L) تحت سمیت آرسنیک

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشیار گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل

2 دانش‌آموخته کارشناسی ارشد اصلاح گیاهان باغی دانشگاه زابل

3 دانشجوی دکتری بیوتکنولوژی، دانشکده کشاورزی دانشگاه زابل

چکیده

تنش ناشی از فلزات سنگین یکی از مهمترین انواع تنش‌ها در اکوسیستم خاک است که باعث کاهش تولید محصول در واحد سطح می‌گردد. امروزه کاربرد ترکیبات فنولی و تنظیم‌کننده رشد گیاه مانند اسید سالیسیلیک به منظور کاهش آثار منفی ناشی تنش‌های مختلف مطرح است. هدف از مطالعه حاضر، ارزیابی تأثیر محلول‌پاشی اسید سالیسیلیک بر سمیت آرسنیک در گیاه ریحان پس از افزودن آرسنیک به خاک بود. این پژوهش در سال ۱۳۹۵ به صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار انجام شد. تیمارهای آزمایشی شامل تنش آرسنیک در چهار سطح صفر (شاهد)، 10، 45، 80 میلی‌گرم در کیلوگرم خاک و محلول‌پاشی اسید سالیسیلیک سه سطح صفر (شاهد)، 1 و 2 میلی‌مولار بود. نتایج نشان داد که افزودن آرسنیک به طور معنی-داری وزن تر و خشک، سطح برگ و رنگیزه‌های فتوسنتزی را کاهش و غلظت کربوهیدارت و آنزیم‌های آنتی اکسیدانی کاتالاز و پراکسیداز را افزایش داد. درحالی که محلول پاشی اسید سالیسیلیک بر تمام صفات تاثیر معنی‌داری داشت؛ به طوری که غلظت 2 میلی‌مولار باعث افزایش وزن تر و خشک، سطح برگ، کلروفیل a، b، کل و کارتنوئید و کاهش فعالیت آنزیم‌های کاتالاز و پراکسیداز شد. در این بررسی اثر متقابل بین اسید سالیسیلیک و آرسنیک بر رنگیزه‌های فتوسنتزی و آنزیم‌های آنتی اکسیدان معنی‌دار بود؛ و اسید سالیسیلیک نقش تعدیل کننده و کاهنده اثر منفی سمیت آرسنیک را بر این خصوصیات داشت. بنابراین با استناد به یافته‌های مطالعه حاضر و همچنین انجام تحقیقات تکمیلی می‌توان محلول‌پاشی اسید سالیسیلیک را جهت بهبود رشد و کاهش سمیت آرسنیک در گیاه دارویی ریحان تحت تنش آرسنیک پیشنهاد کرد.

کلیدواژه‌ها


Agarawal, S., Sairam, R.K., Srivasta, G.C., Meena, R.C., 2005. Changes in antioxidant enzymes activity and oxidative stress by abscisic acid and salicylic acid in wheat genotypes. Biologia Plantarum. 49(4), 541-550.

Akbarian, M.M., Heidari Sharifabad, H., Noormohammadi, G., Darvish Kojouri, F., 2012. The effect of potassium, zinc and iron foliar application on the production of saffron (Crocus sativa). Annals of Biological Research. 3 (12), 5651 - 5658. [In Persian with English Summary].

Ali, B.M., Vajpayee, P., Tripathi, R.D., Rai, U.N., Singht, S.N., Singhgh, S.P., 2003. Phytoremediation of  lead, nickel, and copper by Salix acmophylla boiss: Role of antioxidant enzymes and antixidant substances. Bull Environ Contam Toxicol. 70(3): 462-469.

Amirjani, M.R., Askary Mehrabadi, M., Aziz mohamadi, F., 2016. Effect of ZnO nanoparticles on vegetative factors, elements content and photosynthetic pigments of wheat (Triticum aestivum). Iranian Journal of Plant Biology. 8 (27), 33-48. [In Persian with English Summary].

Arnon, A.N., 1967. Method of extraction of chlorophyll in the plants. Agronomy Journal, 23, 112-121.

Asada, K., 2000. The water-water cycle as alternative photon and electron sinks. Philosophical Transactions of the Royal Society B: Biological Sciences. 355, 1419-1431

Asadi-Karam, A., Karamt, B., Mozafari, H., 2017. Effect of Interaction of Trichontonol and Arsenic on Growth and Some Biochemical and Physiological Characteristics in Soybean. Journal of Plant Research. 30(3), 506-517. [In Persian with English Summary].

Balochi, H. R., Amini, F., Movahedi-Dehnavi, M., Atar-Zadeh, M., 2016. Effect of Different Growing Organic Substrates on Growth and Yield Components of Pinto Bean (Phaseolus vulgaris L.) under Heavy Metals Stress. Journal of Agricultural Science  and Sustainable Production. 26(2), 57-72. [In Persian with English Summary].

Bayat, H., Alirezaie, M., Neamati, H., 2012. Impact of exogenous salicylic acid on growth and ornamental characteristics of calendula (Calendula officinalis L.) under salinity stress. Journal of Stress Physiology and Biochemistry. 8, 258-267.

Beers, G.R., Sizer, I.V., 1952. A spectrophotometric method for measuring the break down of hydrogen peroxide by catalase. Journal of Biological Chemistry. 195, 133-140.

Cao, H., Jiang, Y., Jianjiang, C., Zhang, H., Huang, W., Li, L., Zhang, W., 2009. Arsenic accumulation in ScutellariabaicalensisGeorgi and its effects on plant growth and pharmaceutical components. Journal of Hazardous Materials. 171, 508-513.

Chen, J., Cheng, Z., Zhong, S., 2007. Effect of exogenous salicylic acid on growth and H2O2- Metabolizing enzymes in rice seedlings lead stress. Journal of Environmental Science. 19, 44-49.

Choudhury, A., Lahiri Choudhury, D.K., Desai, A., Duckworth, J.W., Easa, P.S., Johnsingh, A.J.T., Fernando, P., Hedges, S., Gunawardena, M., Kurt, F., Karanth, U., Lister, A., Menon, V., Riddle, H., Rübel, A., Wikramanayake, E. 2008. Elephas maximus. In: IUCN 2008. 2008 IUCN Red List of Threatened Species. accessed Jan. 2009.

Chun-xi, L., Shu-li, F., Yan, S.H., Li-na, J., Xuyang, L., Xiao-li, H., 2007. Effects of arsenic on seed germination and physiological activities of wheat seedlings. Journal of Environmental Science. 19, 725-732.

Connell, S. L., AL-Hamdani, S. H., 2001. Selested physiological responses of Kudzu to different chromium concentration. Canadian Journal of Plant Science. 81, 33-58

Cozzolino, V., Pigna, M., Di Meo, V., Caporale, A.G., Violante, A., Meharg, A.A., 2010. Influence of phosphate addition on the arsenic uptake by wheat (Triticum durum) grown in arsenic polluted soils. Fresenius Environmental Bulletin. 19(5), 838-845.

Dolat-Abadian, A., Sanavi, M., Sharifi, M., 2009. Effect of dehydration and ascorbic acid spraying on the activity of antioxidant enzymes and some biochemical changes in corn seedlings. Journal of Plant Research. 22(3), 407-422. [In Persian with English Summary].

El-Tayeb, M. A. 2005. Response of barley grain to the interactive effect of salinity and salicylic acid. Plant Growth Regulation. 42, 215-224.

Eraslan, F., Inal, A., Pilbeam, DJ., Gunes, A., 2008. Interactive effects of salicylic acid and silicon on oxidative damage and antioxidant activity in spinach (Spinacia oleracea L. CV. Matador) grown under boron toxicity and salinity. Plant Growth Regulation. 55, 207-219.

Eshghi, S., Moharami, S., jamali, B., 2016. Effect of Salicylic Acid on Growth, Function and Quality of Strawberry Fruits in Parus Cultivar under Salinity Conditions. Journal of Crop Science and Technology of Greenhouse Culture. 7(28), 163-173. [In Persian with English Summary].

Esfandiari, E., Shakiba, M.R., Mahboob, S.A., Alyari, H., Shahabivand.,  2008. The Effect of Water Stress on the Antioxidant Content, Protective Enzyme Activities, Proline Content and Lipid Peroxidation in Wheat Seedling. Pakistan Journal of Biological Sciences. 11 (15),  1916-1922.

Fahmideh, L., Mahmoodi, N., 2017. Survey stress on enzymatic activation rate and secondary metabolites of Cuminum cyminum under manganese stress. Journal of Crop Science Research in Arid Regions. 1(2), 191-204.

Fariduddin, Q., Hayat, S., Ahmad, A., 2003. Salicylic acid influences net photosynthetic rate, carboxylation efficiency, nitrate reductase activity, and seed yield in Brassica juncea. Photosynthetica. 41, 281-284. [In Persian with English Summary].

Farzaneh, M., Ghanbari, M., Eftekharian, A.R., Javanmard, Sh.A., 2013. Effect of salicylic acid spraying on the amount of photosynthesis osmolites and photosynthetic pigments of an eggplant under cold stress. Iranian Journal of Plant Ecophysiology. 8(4), 75-83. [In Persian with English Summary].

Fazelian, N., Asrar, Z., 2011. Influence of interactions of arsenic and salicylic acid on growth and some physiological indices of chamomile. Plant Biology. 3(8), 1-12.

Ghai, N., Setia, R. C., Setia, N., 2002. Effect of paclobutrazol and salicylic acid on chlorophyll content, hill activity and yield components in Brassica napus L. Phytomorphol. 52, 83-87.

Gheysari, S., Saied-Nematpoor, F., Safipoor-Afshar, A., 2015. Effect of salicylic acid and ascorbic acid on the content of photosynthetic pigments and activity of some antioxidant enzymes in basil under stress. Journal of Plant Research. 28(4), 814-825. [In Persian with English Summary].

Hegedus, A., Erdei, S., Horvath, G., 2001. Comparative studies of H2O2 detoxifying enzymes in green and greening barley seedlings under cadmium stress. Plant Sciences. 160, 1085–1093.

Holy, M. C., 1972. Indole acetic acid oxidase: a dual catalytic enzyme. Journal of Plant Physiology. 50, 15-18.

Howladar, SM., 2014. A novel moringa oleifera leaf extract can mitigate the stress effects of salinity and cadmium in bean (Phaseoulus vulgaris L.) plants. Ecotoxicology and Environmental Safety. 100. 69-75.

Jamali, B., Eshghi, S., Tafazoli, E., 2011. Vegetative and reproductive growth of strawberry plants cv. ‘Pajaro’ affected by salicylic acid and nickel. Journal of Agricultural Science and Technology. 13, 895-904. [In Persian with English Summary].

Jamali, B., Eshghi, S., Tafazoli, E., 2013. Vegetative growth, yield, fruit quality and fruit and leaf composition of strawberry cv. ‘Pajaro’ as influenced by salicylic acid and nickel sprays. Journal of Plant Nutrition. 36, 1043-1055. [In Persian with English Summary].

Jing, CH., Cheng, Z., Li-ping, L., Zhong-yang, S., Xue-bo, P., 2007. Effects of exogenous salicylic acid on growth and H2O2- metabolizing enzymes in rice seedlings under lead stress. Journal of Environmental Science. 19, 44- 49.

Karimi, N., Souri, Z., 2015. Investigating the interaction between arsenic and phosphorus on chlorophyll content and accumulation of malondialdehyde in Isatis cappadocica. Journal of Plant Process and Function. 4(11), 1-12. [In Persian with English Summary].

Karlidag, H., Yildirim, E., Turan, M., 2009. Salicylic acid ameliorates the adverse effect of salt stress on strawberry. Scientia Agricola. 66, 180-187.

Kerepsi, I., Toth, M., Boross, L., 1996. Water-soluble carbohydrates in dried plant. Journal Agricultur Food Chemical. 10, 3235-3239.

Khodary, S. E. A., 2004. Effect of salicylic acid on the growth, photosynthesis and carbohydrate metabolism in salt stressed Maize Plants. International Journal of Agriculture and Biology. 6, 5-8.

Lichtenthaler, H. K., 1987. Chlorophylls and carotenoids; Pigments of photosynthetic biomembranes. Methods in Enzymology. 48, 350-382.

Lin, T., Zhu, X., Zhang, F., 2012. The Interaction effect of cadmium and Nitrogen on Populus yunnanensis. The Journal of Agricultural Science. 4 (2), 125-134.

Loua, I.Q., Yec, Z.H., Wonga, M.H., 2009. A comparison of arsenic tolerance, uptake and accumulation between arsenic hyper accumulator Pteris vittata L and non-hyperaccumulator P. semipinnata L. A hydroponic study. Hazardous Materials. 171, 436-442

Mahdavian, K., Ghadrian, S. M., Tork-Zadeh Mahani, M., 2015. The Effect of Different Concentrations of Lead on Some Physiological Parameters in Two Populations of Harmal (Peganum harmala L.). Journal of Cell and Tissue. 6(4), 543-555.. [In Persian with English Summary].

Mazarie, A., Mousavi-nik, S. M., Ghanbari, A., Fahmideh, L., 2019. Effect of titanium dioxide spraying on physiological characteristics of sage (Salvia officinalis L.) under water stress. Environmental Stresses in Crop Sciences. 12(2): 539-559. [In Persian with English Summary].

Mazarie, A., Sirousmehr, A.R., Babaei, Z., 2017. Effect of mycorrhizal fungi on some morphological and physiological charactristics of Milk thistle (Silybum marianum (L.) Gaertn.) under drought stress. Iranian Journal of Medicinal and Aromatic Plants. 33 (4), 620-635. [In Persian with English Summary].

Metwally, A., Finkemeier, I., Georgi, M., Dietz, K. J., 2003. Salicylic acid alleviates the cadmium toxicity in Barley seedling. Plant Physiology. 132, 272-281.

Mishra, A., Choudhuri, M.A., 1999. Effect of salicylic acid on heavy metal induced membrane deterioration mediated by lipooxygenases in rice. Biologia Plantarum. 42, 409 – 415.

Mohammadi, S., Hidari, M., Dahmardeh, M., Asgharipoor, M. R., 2015. Effect of different concentrations of lead on some physiological parameters in two populations of spinach. Journal of Cell and Texture. 8(4), 105-120. [In Persian with English Summary].

Morello, J.R., Romero, M.P., Ramo, T., Motilva, M.J., 2005. Evaluation of L-phenylalanine ammonialyase activity and phenolic profile in olive drupe (Olea europaea L.) from fruit setting period to harvesting time. Plant Science. 168, 65-72.

Najafi, F., Khavari-Nezhad, R.A., Rashidi, M., 2016. Effect of sodium selenate on some antioxidant enzymes in sunflower plant under salt stress. Journal of Plant Process and Function. 6(19), 251-263. [In Persian with English Summary].

Norani-Azad, H., Kafil-Zadeh, F., 2011. Effect of cadmium toxicity on growth, soluble sugars, photosynthetic pigments and some enzymes in safflower. Journal of Biology, Iran. 24(6), 858-887. [In Persian with English Summary].

Padash, A., Ghanbari, A., Asgharipoor, M. R., 2016. The interaction of lead and salicylic acid on some quantitative and qualitative growth parameters and antioxidant enzymes of basil. Greenhouse Crop Science and Technology. 7(27), 181-191. [In Persian with English Summary].

Pigna, M., Cozzolino, V., Violanto, A., Meharg A., 2009. Influence of phosphate on the arsenic uptake by wheat (Triticum durum L.) irrigated with arsenic solutions at three different concentrations. Water, Air and Soil Pollution. 197, 371-380.

Pisani, T., Munzi, S., Paoli, A., Bockor, M., Loppi, S., 2010. Physiological effects of arsenic in the (Lichen xanthoriaParietina L.). Chemosphere. 40, 440-454.

Rai, V., Khatoon, S., Bisht, S. S., Mehrotra, S., 2005. Effect of cadmium ongrowth, ultramorphology of leaf and secondary metabolites of Phyllanthus amarus Schum and Thonn.Chemosphere. 61, 1644-1650.

Rashid Shomali, A., Khodaverdiloo, H., Samadi, A., 2012. Accumulation and tolerance to soil cadmium by Pennisetum glausum, Chnopodium album, Portulaca oleracea and Descurainia sophia. Iranian Journal of Soil Management and Sustainable Agriculture. 2(1), 45-62. [In Persian with English Summary].

Sairam, R.K., Rao, K.V., Srivastava. G.C., 2002. Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Science. 163, 1037-1046.

Shaibur, M.R., Kitajima, N., Sugawara, R., Kondo, T., Alam, Sh., Imamul-Huq, S.M., Kawai, Sh., 2008. Critical toxicity level of arsenic and elemental composition of arsenicInduced chlorosis in hydroponic sorghum. Water, Air and Soil Pollution. 191, 279-292.

Sharma, P., Dubey, R. S., 2004. Ascorbat peroxidase from rice seeding: properties of enzyme isoforms, effects of stresses and protective roles of osmolytes. Plant Science. 167(3), 541-550.

Shi, Q., Zhu, Z., 2008. Effects of exogenous salicylic acid on manganese toxicity, element contents and antioxidative system in cucumber. Environmental and Experimental Botany 63, 317-326.

Shri, M., Kumar, S., Chakrabarty, D., Trivedi, P. K., Mallick, S., Misra, P., Shukla, D., Mishra, S., Srivastava, S., Tripathi, R.D., Tuli, R., 2009. Effect of arsenic on growth, oxidative stress, and antioxidant system in rice seedlings. Ecotoxicology and Environmental Safety. 72, 1102-1110.

Singh, H. P., Kaur, S., Batish, D. R., Sharma, V. P., Sharma, N., 2009. Nitric oxide alleviates arsenic toxicity by reducing oxidative damage in the roots of Oryza sativa (rice). Nitric Oxide. 20, 289-297

Srivastava, S., Srivastava, A.K., Suprasanna, P., D’Souza, S.F., 2009. Comparative biochemical and transcriptional profiling of two contrasting varieties of Brassica juncea L. in response t arsenic exposure reveals mechanisms of stress perception and tolerance. Journal of Experimental Botany. 181, 1-13.

Stoeva, N., Bineva, T., 2003. Oxidative changes and photosynthesis in oat plants grown in As-contaminated soil. Bulg. Journal of Plant Physiology. 29 (1-2), 87-95

Szepesi, A., Csiszar, J., Gemes, K., Horvath, E., Horvath, F., Simon, M., Tari, I., 2009. Salicylic acid improves acclimation to salt stress by stimulating abscisic aldehyde oxidase activity and abscisic acid accumulation, and increases Na+ content in leaves without toxicity symptoms in Solanum lycopersicum L. Journal of Plant Physiology. 166, 914-925.

Tabatabaei, S.J. 2009. Mineral Nutrition of Plants. Author Publications, 388p.

Tari, I., Csiszar, J, Szalai, G., Horvath, F., Pecsvaradi, A., Kiss, G., Szepsi, A., Szabo, M., Erdei, L., 2002. Acclimation of tomato plants to salinity stress after a salicylic acid pre-treatment. Acta Biologica Szegediensis. 46 (3-4), 55-56.

Vitoria, A. P., Cunhab, M. D., Azevedo, R. A, 2006. Ultrastructural changes of radish leaf exposed to cadmium. Environmental and Experimental Botany. 58, 47-52.

Wang, H., Zhao, S. C., Liu, R. L., Zhou, W., Jin, J. Y., 2009. Changes of photosynthetic activities of maize (Zea mays L.) seedlings in response to cadmium stress. Photosynthetica. 47(2), 277-283.

Yadav, SK, 2010. Heavy metals toxicity in plants: An overview on the role of glutathione and phytochelatins in heavy metals stress tolerance of plants. South African Journal of Botany. 76, 167-179.

Yadgari, M., Karimi, S., 2014. Effect of heavy metals of cadmium and nickel on yield and traits of medicinal plant purple. Journal of Modern Science in Sustainable Agriculture. 10(1), 83-92. [In Persian with English Summary].

Yadallahi, P., Asgharipoor, M. R., Bagheri, A.A., Jabari, B., Shekhpoor, S., 2013. Effects of different levels of sodium nitroprusside and arsenic on Carla's quantitative characteristics (Momordica charantia L.). Journal of Crop Research. 5(3), 215-225. [In Persian with English Summary].

Yadallahi, P., Asgharipoor, M. R., Bagheri, A.A., Kheri, N.A., Amiri, A., Effect of ascorbic acid on some physiological and biochemical properties of basil under arsenic toxicity. Plant Production Technology. 2016. 16(1), 207-217. [In Persian with English Summary].

Zhou, Z. S., Guo, K., Abdou-Elbaz, A., Yang, Z. M., 2009. Salicylic acid alleviates mercury toxicity by preventing oxidative stress in roots of Medicago sativa.  Environmental and Experimental Botany. 65(1), 27-34