اثر تنش خشکی بر شاخص سطح برگ، فتوسنتز، هدایت روزنه ای و میزان پرولین در دو رقم لوبیاچیتی (.Phaseolus vulgaris L)

نوع مقاله: مقاله پژوهشی

نویسندگان

گروه زراعت و اصلاح نباتات، واحد میاندوآب، دانشگاه آزاد اسلامی، میاندوآب، ایران

چکیده

جهت بررسی اثر تنش خشکی بر شاخص سطح برگ، سرعت فتوسنتز، میزان کلروفیل، هدایت روزنه‌ای و میزان پرولین در ارقام لوبیا چیتی، آزمایشی بصورت فاکتوریل در قالب طرح بلوک‌های کامل تصادفی با چهار تکرار اجرا شد. تیمارهای آزمایش شامل تنش خشکی در سه سطح، آبیاری کامل (شاهد)، قطع آبیاری در مرحله رویشی شامل ظهور سه برگچه اول (V3) و قطع آبیاری در مرحله گلدهی (R6) و دو رقم لوبیا چیتی (تلاش و خمین) بودند. نتایج نشان داد در هر دو رقم لوبیا چیتی شاخص سطح برگ، میزان کلروفیل a و b، سرعت فتوسنتز و هدایت روزنه‌ای در اثر تنش خشکی کاهش معنی دار یافت ولی بر میزان پرولین بطور معنی داری افزوده شد. تحت تنش، بیشترین مقدار کاهش در صفات به ترتیب به محتوای کلروفیل a، شاخص سطح برگ، هدایت روزنه‌ای، سرعت فتوسنتز و محتوای کلروفیل b اختصاص داشت. ارقام لوبیا از لحاظ شاخص سطح برگ و میزان کلروفیل b، تحت شرایط آبیاری کامل فاقد اختلاف معنی‌دار بودند ولی در شرایط اعمال تنش خشکی، رقم تلاش در مقایسه با رقم خمین به طور معنی داری از کاهش کمتر هدایت روزنه‌ای، میزان کلروفیل و سرعت فتوسنتز برخوردار بود. میزان کاهش هدایت روزنه‌ای در تنش خشکی در مراحل گلدهی و رویشی نسبت به شاهد (آبیاری کامل) در رقم تلاش به ترتیب 25.69 و 46.29 درصد و در رقم خمین به ترتیب 38.90 و 57.81 درصد بود و افزایش محتوی پرولین نیز در رقم تلاش به ترتیب 56.46 و 92.51 درصد و در رقم خمین به ترتیب 43.97 و 81.45 درصد بدست آمد. نتیجه گیری شد رقم تلاش با دوام فتوسنتزی بیشتر از طریق حفظ سطح برگ، هدایت روزنه‌ای، سرعت فتوسنتز و میزان کلروفیل و افزایش مقدار پرولین در شرایط تنش خشکی مقاوم‌تر از رقم خمین است.

کلیدواژه‌ها


Abaslou, L., Kazemini, S.A., Adalat, M., Dadkhodaei, A., 2015. Effect of drought stress and planting pattern on some physiological and biochemistry characteristics of two chickpea cultivars. Journal of Crop Agriculture. 16(4), 933-943. [In Persian with English summary].

Akhkha, A., Boutraa, T., Alhejely, A., 2011. The rates of photosynthesis, chlorophyll content, dark respiration, proline and abscicic acid (ABA) in wheat (Triticum durum) under water deficit conditions. International Journal of Agriculture and Biology. 13, 215–221.

Anjorin, F.B., Adejumo, S.A., Agboola, L., Samuel, Y.D., 2016. Proline, soluble sugar, leaf starch and relative water contents of four maize varieties in response to different watering regimes. Cercetari Agronomice in Moldova. 3(167), 51-62.

Anjum, S.A., Farooq, M., Wang, L.C., Xue, L.L., Wang, S.G., Wang, L., Zhang, S., Chen, M., 2011a. Gas exchange and chlorophyll synthesis of maize cultivars are enhanced by exogenously-applied glycinebetaine under drought conditions. Journal of Plant, Soil and Environment. 57(7), 326–331.

Anjum, S.A., Xie, X.Y., Wang, L.C., Saleem, M.F., Man, C., Lei, W., 2011b. Morphological, physiological and biochemical responses of plants to drought stress. African Journal of Agricultural Research. 6(9), 2026-2032.

 Anjum, S.A., Farooq, M., Xie, X.Y., Ijaz, M.F., 2012. Antioxidant defense system and proline accumulation enables hot pepper to perform better under drought. Journal of Scientia Horticulturae. 140, 66-73.

Ardabili, A. A., Sadeghipour, O., Rashidi Asl, A., 2013.The effect of proline application on drought tolerance of cowpea (Vigna unguiculata L.). Advances in Environmental Biology. 7(14), 4689-4696.

Ashraf, M., Harris, P.J.C., 2013. Photosynthesis under stressful environments: An overview. International Journal for Photosynthesis Research. 51(2), 163-190.

Ashraf, M.Y., Azmi, A.R., Khan, A.H., Ala, S.A., 1994. Effect of water stress on total phenols, peroxidase activity and chlorophyll content in wheat. Acta Physiologiae Plantarum. 16(3), 185-191.

Bates, L.S., Waldren, R.P., Teare, I.D., 1973. Rapid determination of free proline for water stress studies. Plant and Soil. 39, 205-208.

Cortes, A.J., Thiis, D., Chavarro, C., Madrinan, S., Blair M.W., 2012. Nucleotide diversity patterns at the drought-related DREB2 encoding genes in wild and cultivated common bean (Phaseolus vulgaris L.). Theoretical and Applied Genetics. 125, 1069-1085.

Fallah, A., Farahmanfar, E., Moradi, F., 2014. Effect of salt stress on some morphophysiological characters of two rice cultivars during different growth stages at greenhouse. Agronomy Journal. 107, 175-182. [In Persian with English summary].

Fallahi, g., Hatami, A., Naseri, R., 2013. Growth Analysis of Six Corn Hybrids Under Drought Conditions in Kermanshah Province, Iran. Journal of Crop Ecophysiology. 7(2), 181-196. [In Persian with English summary].

Gregersen, P.L., Holm, P.B., 2007. Transcriptome analysis of senescence in the flag leaf of wheat. Journal of Plant Biotechnology. 5, 192-206.

Grzesıak, M.T., Grzesıak, S., Skoczowsk, A., 2006. Changes of leaf water potential and gas exchange during and after drought in triticale and maize genotypes differing in drought tolerance. International Journal for Photosynthesis Research. 44(4), 561-568.

Han, J.M., Meng, H.F., Wang, S.Y., Jiang, C.D., Liu, F., Zhang, W.F., Zhang, Y.L., 2016. Variability of mesophyll conductance and its relationship with water use efficiency in cotton leaves under drought pretreatment. Journal of Plant Physiology. 194, 61-71.

Homayouni, H., Khazarian, V., 2014. Effect of deficit irrigation on soluble sugars, starch and proline in three corn hybrid. Indian. Journal of Scientific Research. 7(1), 910-917.

Hu, H., Xiong, L., 2014. Genetic engineering and breeding of drought resistant crops. Journal of Plant Biology. 65, 715-741.

Jabasingh, C., Babu, S., 2013. Proline Content of Oryza sativa L. under Water Stress. Journal of Academia and Industrial Research. 2(7), 442-445.

Jaleel, C.A., Manivannan, P., Wahid, A., Farooq, M., Al-juburi, H.J., Somasundaram, R., Panneerselva, A.M., 2009. Drought stress plants: a review on morphological characteristics and pigments composition. International Journal of Agriculture and Biology. 11, 100-105.

Jurekova, Z., Molnar, K.N., Paganova, V., 2011. Physiological responses of six tomato (Lycopersicon esculentum Mill.) cultivars to water stress. Journal of Horticulture and Forestry. 3(10), 294-300.

Kaur, G., Singh, H.P., Batish, D.R., Kohli, R.K., 2012. Growth, photosynthetic activity and oxidative stress in wheat (Triticum aestivum) after exposure of lead to soil. Journal of Environmental Biology. 33, 265-269.

Korir, P.C., Nyabundi, J.O., Kimurto, P.K., 2006. Genotypic responses of common bean (Phaseolus vulgaris L.) to moisture stress conditions in Kenya. Asian Journal of Plant Science. 5, 24-32.

Maazou, A.R.S., Tu, J., Qiu, J., Liu, Z., 2016. Breeding for Drought Tolerance in Maize (Zea mays L.). American Journal of Plant Sciences. 7, 1858-1870.

Kandil, A., Sharief, A.E., El-Mohandes, S.I., Keshta, M.M., 2017. Performance of canola (Brassica napus l.) genotypes under drought stress. International Journal of Environment, Agriculture and Biotechnology. 2(2), 653-661.

Khayatnezhad, M., Gholamin, R., 2012. The effect of drought stress on leaf chlorophyll content and stress resistance in maize cultivars (Zea mays). African Journal of Microbiology Research. 6(12), 2844-2848.

Kusvuran, S., 2012. Effects of drought and salt stresses on growth, stomatal conductance, leaf water and osmotic potentials of melon genotypes (Cucumis melo L.). African Journal of Agricultural Research. 7(5), 775-781.

Mahpara, S., Hussain, T., Farooq, J., 2014. Drought tolerance studies in wheat (Triticum aestivum L.). Cercetari Agronomice in Moldova. 4(160), 133-140.

Mafakheri, A., Siosemardeh, A., Bahramnejad1, B., Struik, P.C., Sohrabil, Y., 2010. Effect of drought stress on yield, proline and chlorophyll contents in three chickpea cultivars. Australian Journal of a Crop Science. 4(8), 580-585.

Magalhaes, I.D., Lyra, G.B., Souza, J.L., Teodora, I., Cavalcante, C.A., Ferreira, R.A., Souza, R.C., 2017.Physiology and Grain Yield of Common Beans under Evapotranspirated Water Reposition Levels. Irrigation and Drainage Systems Engineering. 6(1), 2-8.

Majidi, M.M., Rashidi, F., Sharafi, Y., 2015. Physiological traits related to drought tolerance in Brassica. International Journal of Plant Production. 9(4), 541-549.

Massacci, A., Nabiev, S.M., Pietrosanti, L., Nematov, S.K., Chernikova, T.N., Thor, K., Leipner, J., 2008. Response of the photosynthetic apparatus of cotton (Gossypium hirsutum) to the onset of drought stress under field conditions studied by gas-exchange analysis and chlorophyll fluorescence imaging. Journal of Plant Physiology and Biochemistry. 46, 189-195.

Mauad, M., Crusciol, C.A.C., Nascente,A.S., Filho, H.G., Lima, G.P., 2016. Effects of silicon and drought stress on biochemical characteristics of leaves of upland rice cultivars. Revista Ciencia Aagronomica Journal. 47(3), 532-539.

Nehbandani, A., Soltani, A., Darvishirad, P., 2015. Effect of terminal drought stress on water use, growth and yield of chickpea (Cicer arietinum L.). Journal of plant ecophysiology. 7(23), 18-27. [In Persian with English summary].

Nohong, B., Nompo, S., 2015. Effect of water stress on growth, yield, proline and soluble sugars contents of Signal grass and Napier grass species. American merican-eurasian journal of sustainable agriculture. 9(5), 14-21.

Oukarroum, A., Schansker, G., Strasser, R.J., 2009. Drought stress effects on photosystem I content and photosystem II thermotolerance analyzed using Chl a fluorescence kinetics in barley varieties differing in their drought tolerance. Physiologia Plantarum.137, 188-199.

Ouyang, W., Struik, P.C., Yin, X., Yang, J., 2017. Stomatal conductance, mesophyll conductance, and transpiration efficiency in relation to leaf anatomy in rice and wheat genotypes under drought. Experimental Botany. 68(18), 5191–5205.

Praba, M.L., Cairns, J.E., Babu, R.C., Lafitte, H.R., 2009. Identification of physiological traits underlying cultivar differences in drought tolerance in rice and wheat. Journal of Agronomy and Crop Science. 195, 30-46.

Rad, M.R.N., Kadir, M.A., Yusop, M.R., 2012. Genetic behaviour for plant capacity to produce chlorophyll in wheat (Triticum aestivum) under drought stress. Australian Journal of Crop Science. 6(3), 415-420.

Rasheed, M., Hussain, A., Mahnood, T., 2003. Growth analysis of hybrid maize as influenced by planting techniques and nutrient management. Agriculture Biology. 5(2), 168-171.

Sharifi, P., Mohammadkhani, N., 2016. Effects of drought stress on photosynthesis factors in wheat genotypes during anthesis. Journal of Cereal Research Communications. 44(2), 229-239.

Sorkhi, F. Effect of irrigation and nitrogen fertilizer levels on physiological characteristics of four varieties of barley (Hordeum vulgare L.). Journal of Crop Ecophysiology. 9(3), 401-416. [In Persian with English summary].

Sultan, M.A., Hui, L., Yang, L.J., Xian, Z.H., 2012. Assessment of drought tolerance of some Triticum L. species through physiological indices. Czech Journal of Genetics and Plant Breeding. 48(4), 178-184.

Siddique, Z., Jan, S., Imadi, S.R., Ahmad, P., 2016. Drought stress and photosynthesis in plants. Journal of Water Stress and Crop Plants. 32, 1-11.

Siddiqui, M.H., Khaishany, M.Y., Qutami, M.A., Whaibi, M.H., Grover, A., Ali, H.M., Wahibi, M.S., Bukhari, N.A., 2015. Response of different genotypes of faba bean plant to drought stress. International Journal of Molecular Sciences. 16, 10214-10227.

 Steiner, M., Toth, E.G., Juhasz, A., Dioszegi, M.S., Hrotko, K., 2014. Stomatal responses of drought and heat stressed linden (Tilia sp.) leaves. Horticulture and Landscape Engineering. 6, 7-10.

Tatrai, Z.A., Sanoubar, R., Pluhar, Z., Mancarella, S., Orsini, F., Gianquinto, G., 2016. Morphological and physiological plant responses to drought stress in Thymus citriodorus. International Journal of Agronomy. 10, 1-8.

Tosens, T., Niinemets, U., Vislap, V., Eichelmann, H., Castro, D.P., 2012. Developmental changes in mesophyll diffusion conductance and photosynthetic capacity under different light and water availabilities in Populus tremula: how structure constrains function. Journal of Plant, Cell and Environment. 35, 839-856.

Vanaja, M., Yadav, S.K., Archana, G., Lakshmi, N.J., Reddy, R.P., Vagheera, P., Razak, S.K., Maheswari, M., Venkateswarlu, B., 2011. Response of C4 (maize) and C3 (sunflower) crop plants to drought stress and enhanced carbon dioxide concentration. Journal of Plant, Soil and Environment. 57(5), 207–215.

Wondimu, W., Tana, T., 2017. Yield response of common bean (Phaseolus vulgaris L.) varieties to combined application of nitrogen and phosphorus fertilizers at Mechara, Eastern Ethiopia.Journal of Plant Biology and Soil Health. 4(2), 2-7.

Wu, F.Z., Bao, W.K., Li, F.L., Wu, N., 2008. Efects of water stress and nitrogen supply on leaf gas exchange and fluorescence parameters of Sophora davidii seedling. International Journal of Photosynthesis Research. 46(1), 40-48.

Zali, H., Hasanloo, T., Sofalian, O., Asghari, A., Zeinalabedini, M., 2016. Drought stress effect on physiological parameter and amino acids accumulations in canola. Journal of Crop Breeding. 8(18), 191-203. [In Persian with English summary].

Zegaoui, Z., Planchais, S., Cabassa, S., Djebbar, R., Belbachir, O.A., Carol, P., 2017. Variation in relative water content, proline accumulation and stress gene expression in two cowpea landraces under drought. Journal of Plant Physiology. 218, 26-34.