نقش تیمارهای اسید سالیسیلیک و پرولین بر القاء فعالیت آنزیم‌های آنتی‌اکسیدانی و پاسخ‌های تحمل به تنش شوری در سویا (.Glycine max L.)

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی گیاهان زراعی، دانشکده کشاورزی، دانشگاه شهرکرد

2 دانشیار گروه زراعت دانشکده کشاورزی، دانشگاه شهرکرد

3 استاد گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه صنعتی اصفهان

چکیده

شوری آب یا خاک یکی از مهم­ترین تنش­ ها در مناطق خشک و نیمه‌خشک است که به‌شدت رشد گیاهان را از طریق تأثیر بر فرآیندهای فیزیولوژیک محدود می­کند. آزمایش حاضر با هدف بررسی بهبود تحمل به شوری سویا با محلول­ پاشی پرولین و اسیدسالیسیلیک در سال 1395 در فضای باز گلخانه ­های دانشگاه شهرکرد در جعبه­ های کاشت با چهار تکرار به‌صورت اسپیلیت پلات در قالب طرح کاملاً تصادفی اجرا شد. عامل اصلی شامل شوری در سه سطح (0، 50 و 100 میلی­مولار) و عامل فرعی محلول ­پاشی پرولین 10 میلی ­مولار، اسید سالیسیلیک 3 میلی‌مولار، ترکیب اسید سالیسیلیک 3 میلی ­مولار با پرولین 10 میلی­مولار، و شاهد (محلول ­پاشی با آب) بود. صفات پرولین، فعالیت آنزیم­ های آنتی­ اکسیدانی، مالون­ دآلدئید، پراکسید هیدروژن، ارتفاع و وزن خشک اندام هوایی اندازه­ گیری شدند. نتایج نشان داد اثرات محلول­ پاشی پرولین و اسید سالیسیلیک به‌طور چشمگیری محتوای پرولین، فعالیت آنزیم ­های آنتی­ اکسیدانی، ارتفاع و وزن خشک اندام هوایی را در سویا تحت تنش شوری افزایش داد. به­ علاوه، تحت شوری 100 میلی­مولار، محلول­ پاشی پرولین و اسید سالیسیلیک باعث کاهش مالون ­دآلدئید و پراکسیدهیدروژن به ترتیب 23 و 25 درصد در مقایسه با تیمار محلول­ پاشی با آب شدند. همچنین ارتفاع و وزن خشک اندام هوایی در سطح شوری 100 میلی­ مولار با کاربرد پرولین و اسیدسالیسیلیک به میزان 32 و 38 درصد نسبت به محلول ­پاشی با آب افزایش یافتند. بنابراین نتایج این مطالعه نشان می­دهد کاربرد پرولین و اسیدسالیسیلیک همراه باهم، تحمل به تنش شوری در سویا را از طریق فعال کردن سیستم دفاعی آنتی ­اکسیدانی و کاهش پراکسیداسیون لیپیدهای غشا بهبود می­دهد.

کلیدواژه‌ها


Aebi, H. 1984. Catalase in vitro. Methods in Enzymology. 105, 121-126.

Ali, G., Srivastava, P. S., Iqbal, M., 1999. Proline accumulation, protein pattern and photosynthesis in regenereants grown under NaCl stress. Biology of Plants. 42, 89-95.

Ashraf, M., Harris, P.J., 2004. Potential biochemical indicators of salinity tolerance in plants. Plant Science. 166, 3-16.

Azari, A., Modares Sanavi, S.A.M., Askari, H., Ghanati, F., Naji, A.M., Alizadeh, B., 2012. Effect of salt stress on morphological and physiological traits of two species of rapeseed (Brassica napus and B. rapa). Journal Field Crop Science. 14(2), 121-135. [In Persian with English Summary].

Bakry, B.A., Taha, M.H., Abdelgawad, Z.A., Abdallah, M.M.S., 2014. The Role of humic acid and proline on growth, chemical constituents and yield quantity and quality of three Flax cultivars grown under saline soil conditions. Agricultural Sciences. 5, 1566-1575.

Bates, L.S., Walden, R.P., Teave I.D., 1973. Rapid determination of free proline for water stress studies. Plant and Soil. 39, 205-207.

Borzouei, A., Kafi, M., Akbari-Ghogdi, E., Mousavi-Shalmani, M.A., 2012. Long term salinity stress in relation to lipid peroxidation super oxide dismutase activity and proline content of salt sensitive and salt-tolerant wheat cultivars. Chili Journal of Agriculture Research. 72(4), 476-482.

Bowler, C., Van Montagu, M., Inze´, D., 1992. Superoxide dismutase and stress tolerance. Plant Physiology and Plant Molecular Biology. 43, 83-116.

Daneshmand, F., Arvin M.J., Keramat, B., Momeni, N., 2012. Interactive effects of salt stress and salicylic acid on germination and plant growth parameters of maize (Zea mays L.) under field conditions. Journal of Plant Process and Function. 1(1), 56-70. [In Persian with English Summary].

Deef, H.E., 2007. Influence of salicylic acid on stress tolerance during seed germination of Triticum aestivum and Hordeum vulgare. Advances in Biological Research.1, 40-48.

Ehsanzadeh, P., Sabagh Nekoonam, M., Nouri, J., Pourhadian, H., Shaydaee, S., 2009. Growth, chlorophyll and cation concentration of tetraploid genotypes. Journal of Plant Nutrition. 23(1), 58-70.

Eraslan, F., Inal, A., Gunes, A., Alpaslan, M. 2007. Impact of exogenous salicylic acid on growth, antioxidant activity and physiology of carrot plants subjected to combined salinity and boron toxicity. Scientia Horticulturae. 113, 120–128.

Eskandari Zanjani, K., Shirani Rad, A.H., Moradi Agdam, A., TaherKhani, T., 2013. Effect of Salicylic Acid Application under Salinity Conditions on Physiologic and Morphologic Characteristics of Artemisia (Artemisia annua L.). Journal of Crop Ecophysiology. 6(4), 415-428. [In Persian with English Summary].

Faraji, A., Reisi, S., Kiani, A.R., Yones Abadi, M., Sadeghnejad, H.R., Kia, Sh., Bagheri, M., Kazemi Talachi, M., Hezarjiribi, E., Mosa Khani, A., Sokht Sarai, N. 2015. Technical instruction of soybean production in Guilan state. Agricultural and Natural Resources Research Center of Guilan Province. Pp.30. [In Persian].

Fayez, K.A., Bazaid, S.A., 2014. Improving drought and salinity tolerance in barley by application of salicylic acid and potassium nitrate. Journal of the Saudi Society of Agricultural Science. 13, 45–55.

Halliwell, B. 1999. Antioxidant defense mechanism from the beginning to the end.Free Radical Research. 31, 261-272.

Harfouche, A.L., Rugini, E., Mencarelli, F., Botondi, R., Muleo, R., 2008. Salicylic acid induces H2O2 production and endochitinase gene expression but not ethylene biosynthesis in Castanea sativa in vitro model system. Journal of Plant Physiology. 165, 734-744.

Hasanuzzaman, M., Nahar, K., and Fujita, M., 2013. Plant response to salt stress and role of exogenous protectants to mitigate salt-Induced damages in ecophysiology and responses of plants under salt stress. Springer New York. Pp. 25-87.

Hayat, Sh., Hayat, Q., Alyemeni, M. N., Wani, A. Sh., Pichtel, J., Ahmad, A., 2012. Role of proline under changing environments. A review. Plant Signaling Behavior. 7(11), 1456-1466.

Hayat, Q., Hayat, S., Irfan, M., Ahmad, A., 2010. Effect of exogenous Salicylic acid under changing environment, A review. Environmental and Experimental Botany. 68, 14-25.

Heath, R.L., Packer L. 1968. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics. 125, 189–198.

Hong, Z., Lakkineni, K., Zhang, Z., Verma, D. P., 2000. Removal of feedback inhibition of delta (1)-pyrroline-5-carboxylate synthetase results in increased proline accumulation and protection of plants from osmotic stress. Plant Physiology. 122, 1129-36.

Hoque, M.A., Banu, M.N., Okuma, E., Amako, K., Nakamura, Y., Shimoishi, Y., 2007. Exogenous proline and glycinebetaine increase NaCl-induced ascorbate- glutathione cycle enzyme activities, and proline improves salt tolerance more than glycinebetaine in tobacco Bright Yellow-2 suspension-cultured cells. Journal of Plant Physiology. 164, 1457-68.

Islam, M.M., Hoque, M.A., Okuma, E., Banu, V., Shimoishi, Y., Nakamura, Y., 2009. Exogenous proline and glycinebetaine increase antioxidant enzyme activities and confer tolerance to cadmium stress in cultured tobacco cells. Journal of Plant Physiology. 166, 1587- 97.

Jasim, A.H., Abu Al- Timmen, W.M., Al- Alwani, B.A., 2012. Effect of salt stress, application of salicylic acid and proline on enzymes activity of sweet pepper (Capsicum annum L.). Protection of environment and water quality, the basis for agricultural production. Food Security sustainable development. 285- 297.

Kang, G. 2003. Salicylic acid changes activities of H2o2 metabolizing enzymes and increases the chilling tolerance of banana seedlings. Environmental and Experimental Botany. 50, 9-15

Kaya, C., Tuna, A.L., Ashraf, M., Altunlu, H., 2007. Improved salt tolerance of melon (Cucumis melo L.) by the addition of proline and potassium nitrate. Environmental and Experimental Botany. 60(3), 397-403.

Kaul. S., Sharma, V., Mehta, V., 2008. Free radical scavenging potential of L-proline, evidence from in vitro assays. Amino Acids. 34, 315-20.

Khedr, A.H.A., Abbas, M.A., Wahid, A.A.A., Quick, W.P., Abogadallah, G.M., 2003. Proline induces the expression of salt-stress-responsive proteins and may improve the adaptation of Pancratium maritimum L. to salt-stress. Journal of Experimental Botany. 54, 2553–2562.

Kohler, J., Antonio Hernández, J., Caravaca, F., Roldán, A., 2009. Induction of antioxidant enzymes is involved in the greater effectiveness of a PGPR versus AM fungi with respect to increasing the tolerance of lettuce to severe salt stress. Environmental and Experimental Botany. 65, 245–252.

Läuchli, A., Luttge, U., 2002. Salinity, Environment-Plants-Molecules. The Netherlands, Kluwer Academic Publishers.

Matysik, J., Alia, B., and Mohanty, P., 2002. Molecular mechanisms of quenching of reactive oxygen species by proline under stress in plants. Current Science. 82(5), 525-532.

Munns, R., 2005. Genes and salt tolerance, bringing them together. New Phytologist.  167, 645-63.

Nakano, Y., Asada, K., 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiology. 22, 867-880.

Nasir Khan, M., Siddiqui, M.H., Mohammad, F., Masroor, M., Khan, A., Naeem, M., 2007. Salinity induced change in growth, enzyme activities, photosynthesis, proline accumulation and yield in linseed genotypes. Journal of Agriculture and Science. 3, 685-689.

Okuma, E., Soeda, K., Tada, M., Murata, Y., 2000. Exogenous proline mitigates the inhibition of growth of Nicotiana tabacum cultured cells under saline conditions. Soil Science and Plant Nutrition. 46, 257-63.

Plewa, M.J., Smith, S.R., Wagne, E.D., 1991. Diethyl dithiocarbamate suppresses the plant activation of aromatic amines into mutagens by inhibiting tobacco cell peroxidase. Mutation Res/Fund Molecular Mechanisms Mutagenesis. 247, 57-64.

Ramezannezhad, R., Lahouti, N., Ganjali, A., 2013. Effect of salicylic acid on physiological and biochemical parameters on resistant and sensitive chickpea (Cicer arietinum L.) genotypes under drought stress. Journal of plant Ecophysiology. 5(12), 24-36. [In Persian with English Summary].

Pashaee, Kh., Raiesi, S., Masoumi, A., Mostafavi, E., Shahkoomahalli, A., 2014. Effect of different level of salinity stress on some morphological and yield of different varieties of soybean. Journal of Oilseed Crops. 3, 75-88.

Rao, A., Ahmad, S.D., Sabir, S.M., Awan, S.I., Hussain Shah, A., Abbas, S.R., Shafique, S., Khan, F., Chaudhary, A., 2013. Potential antioxidant activities improve salt tolerance in ten varieties of Wheat (Triticum aestivum L.). American Journal of Plant Sciences. 4, 69-76.

Shahbazizadeh, E., Movahhedi Dehnavi, M., Balouchi, H.R., 2015. Effects of foliar application of salicylic and ascorbic acids on some physiological characteristics of soybean (cv. Williams) under salt stress. Journal of Plant Process and Function. 4 (11), 13-22. [In Persian with English Summary].

Shokrpour, M., Esfandiari, E., 2014. Grouping Different Wheat Varieties for Salt Tolerance using Some Biochemical and Physiological Indices. Journal of Crop Breeding. 6(14), 54-66.

Skopelitis, D.S., Paranychianakis, N.V., Paschalidis, K.A., Pliakonis, E.D., Delis, I. D., Yakoumakis, D.I., 2006. Abiotic stress generates ROS that signal expression of anionic glutamate dehydrogenases to form glutamate for proline synthesis in tobacco and grapevine. The Plant Cell. 18(10), 2767-2781.

Vafabakhsh, J., Nasiri Mahalati, M., Kochaki, A., 2008. Impact of water stress on yield and radiation use efficiency of canola (Berasica napus). Journal of Field Crops Research. 6, 193-208. [In Persian with English Summary].

Velikova, V., Yordanov, I., Edreva, A., 2000. Oxidative stress and some antioxidant systems in acid rain-treated bean plants, protective role of exogenous polyamines. Plant Science. 151, 59-66.